diff --git a/.chloggen/2726-non-root-nginx-autoinstrumentation.yaml b/.chloggen/2726-non-root-nginx-autoinstrumentation.yaml
new file mode 100755
index 0000000000..04310f0375
--- /dev/null
+++ b/.chloggen/2726-non-root-nginx-autoinstrumentation.yaml
@@ -0,0 +1,16 @@
+# One of 'breaking', 'deprecation', 'new_component', 'enhancement', 'bug_fix'
+change_type: 'bug_fix'
+
+# The name of the component, or a single word describing the area of concern, (e.g. collector, target allocator, auto-instrumentation, opamp, github action)
+component: auto-instrumentation
+
+# A brief description of the change. Surround your text with quotes ("") if it needs to start with a backtick (`).
+note: "Fix file copy for NGINX auto-instrumentation for non-root workloads."
+
+# One or more tracking issues related to the change
+issues: [2726]
+
+# (Optional) One or more lines of additional information to render under the primary note.
+# These lines will be padded with 2 spaces and then inserted directly into the document.
+# Use pipe (|) for multiline entries.
+subtext:
diff --git a/pkg/instrumentation/nginx.go b/pkg/instrumentation/nginx.go
index 80b53d8295..350a3533b9 100644
--- a/pkg/instrumentation/nginx.go
+++ b/pkg/instrumentation/nginx.go
@@ -186,7 +186,7 @@ NGINX_SID_VALUE=$5 \n
 echo "Input Parameters: $@" \n
 set -x \n
 \n
-cp -ar /opt/opentelemetry/* ${NGINX_AGENT_DIR_FULL} \n
+cp -r /opt/opentelemetry/* ${NGINX_AGENT_DIR_FULL} \n
 \n
 NGINX_VERSION=$(cat ${NGINX_AGENT_CONF_DIR_FULL}/version.txt) \n
 NGINX_AGENT_LOG_DIR=$(echo "${NGINX_AGENT_DIR_FULL}/logs" | sed 's,/,\\/,g') \n
diff --git a/pkg/instrumentation/nginx_test.go b/pkg/instrumentation/nginx_test.go
index 91d2a9d8c8..65a9d5b8a0 100644
--- a/pkg/instrumentation/nginx_test.go
+++ b/pkg/instrumentation/nginx_test.go
@@ -28,7 +28,7 @@ import (
 
 var nginxSdkInitContainerTestCommand = "echo -e $OTEL_NGINX_I13N_SCRIPT > /opt/opentelemetry-webserver/agent/nginx_instrumentation.sh && chmod +x /opt/opentelemetry-webserver/agent/nginx_instrumentation.sh && cat /opt/opentelemetry-webserver/agent/nginx_instrumentation.sh && /opt/opentelemetry-webserver/agent/nginx_instrumentation.sh \"/opt/opentelemetry-webserver/agent\" \"/opt/opentelemetry-webserver/source-conf\" \"nginx.conf\" \"<<SID-PLACEHOLDER>>\""
 var nginxSdkInitContainerTestCommandCustomFile = "echo -e $OTEL_NGINX_I13N_SCRIPT > /opt/opentelemetry-webserver/agent/nginx_instrumentation.sh && chmod +x /opt/opentelemetry-webserver/agent/nginx_instrumentation.sh && cat /opt/opentelemetry-webserver/agent/nginx_instrumentation.sh && /opt/opentelemetry-webserver/agent/nginx_instrumentation.sh \"/opt/opentelemetry-webserver/agent\" \"/opt/opentelemetry-webserver/source-conf\" \"custom-nginx.conf\" \"<<SID-PLACEHOLDER>>\""
-var nginxSdkInitContainerI13nScript = "\nNGINX_AGENT_DIR_FULL=$1\t\\n\nNGINX_AGENT_CONF_DIR_FULL=$2 \\n\nNGINX_CONFIG_FILE=$3 \\n\nNGINX_SID_PLACEHOLDER=$4 \\n\nNGINX_SID_VALUE=$5 \\n\necho \"Input Parameters: $@\" \\n\nset -x \\n\n\\n\ncp -ar /opt/opentelemetry/* ${NGINX_AGENT_DIR_FULL} \\n\n\\n\nNGINX_VERSION=$(cat ${NGINX_AGENT_CONF_DIR_FULL}/version.txt) \\n\nNGINX_AGENT_LOG_DIR=$(echo \"${NGINX_AGENT_DIR_FULL}/logs\" | sed 's,/,\\\\/,g') \\n\n\\n\ncat ${NGINX_AGENT_DIR_FULL}/conf/opentelemetry_sdk_log4cxx.xml.template | sed 's,__agent_log_dir__,'${NGINX_AGENT_LOG_DIR}',g'  > ${NGINX_AGENT_DIR_FULL}/conf/opentelemetry_sdk_log4cxx.xml \\n\necho -e $OTEL_NGINX_AGENT_CONF > ${NGINX_AGENT_CONF_DIR_FULL}/opentelemetry_agent.conf \\n\nsed -i \"s,${NGINX_SID_PLACEHOLDER},${OTEL_NGINX_SERVICE_INSTANCE_ID},g\" ${NGINX_AGENT_CONF_DIR_FULL}/opentelemetry_agent.conf \\n\nsed -i \"1s,^,load_module ${NGINX_AGENT_DIR_FULL}/WebServerModule/Nginx/${NGINX_VERSION}/ngx_http_opentelemetry_module.so;\\\\n,g\" ${NGINX_AGENT_CONF_DIR_FULL}/${NGINX_CONFIG_FILE} \\n\nsed -i \"1s,^,env OTEL_RESOURCE_ATTRIBUTES;\\\\n,g\" ${NGINX_AGENT_CONF_DIR_FULL}/${NGINX_CONFIG_FILE} \\n\nmv ${NGINX_AGENT_CONF_DIR_FULL}/opentelemetry_agent.conf  ${NGINX_AGENT_CONF_DIR_FULL}/conf.d \\n\n\t\t"
+var nginxSdkInitContainerI13nScript = "\nNGINX_AGENT_DIR_FULL=$1\t\\n\nNGINX_AGENT_CONF_DIR_FULL=$2 \\n\nNGINX_CONFIG_FILE=$3 \\n\nNGINX_SID_PLACEHOLDER=$4 \\n\nNGINX_SID_VALUE=$5 \\n\necho \"Input Parameters: $@\" \\n\nset -x \\n\n\\n\ncp -r /opt/opentelemetry/* ${NGINX_AGENT_DIR_FULL} \\n\n\\n\nNGINX_VERSION=$(cat ${NGINX_AGENT_CONF_DIR_FULL}/version.txt) \\n\nNGINX_AGENT_LOG_DIR=$(echo \"${NGINX_AGENT_DIR_FULL}/logs\" | sed 's,/,\\\\/,g') \\n\n\\n\ncat ${NGINX_AGENT_DIR_FULL}/conf/opentelemetry_sdk_log4cxx.xml.template | sed 's,__agent_log_dir__,'${NGINX_AGENT_LOG_DIR}',g'  > ${NGINX_AGENT_DIR_FULL}/conf/opentelemetry_sdk_log4cxx.xml \\n\necho -e $OTEL_NGINX_AGENT_CONF > ${NGINX_AGENT_CONF_DIR_FULL}/opentelemetry_agent.conf \\n\nsed -i \"s,${NGINX_SID_PLACEHOLDER},${OTEL_NGINX_SERVICE_INSTANCE_ID},g\" ${NGINX_AGENT_CONF_DIR_FULL}/opentelemetry_agent.conf \\n\nsed -i \"1s,^,load_module ${NGINX_AGENT_DIR_FULL}/WebServerModule/Nginx/${NGINX_VERSION}/ngx_http_opentelemetry_module.so;\\\\n,g\" ${NGINX_AGENT_CONF_DIR_FULL}/${NGINX_CONFIG_FILE} \\n\nsed -i \"1s,^,env OTEL_RESOURCE_ATTRIBUTES;\\\\n,g\" ${NGINX_AGENT_CONF_DIR_FULL}/${NGINX_CONFIG_FILE} \\n\nmv ${NGINX_AGENT_CONF_DIR_FULL}/opentelemetry_agent.conf  ${NGINX_AGENT_CONF_DIR_FULL}/conf.d \\n\n\t\t"
 
 func TestInjectNginxSDK(t *testing.T) {
 
diff --git a/tests/e2e-instrumentation/instrumentation-nginx/01-install-app.yaml b/tests/e2e-instrumentation/instrumentation-nginx/01-install-app.yaml
index 13304afb75..3ca305fa63 100644
--- a/tests/e2e-instrumentation/instrumentation-nginx/01-install-app.yaml
+++ b/tests/e2e-instrumentation/instrumentation-nginx/01-install-app.yaml
@@ -42,7 +42,6 @@ spec:
             mountPath: /etc/nginx/nginx.conf
             subPath: nginx.conf
             readOnly: true
-        imagePullPolicy: Always
         resources:
           limits:
             cpu: "1"