diff --git a/.github/workflows/publish-autoinstrumentation-e2e-images.yaml b/.github/workflows/publish-autoinstrumentation-e2e-images.yaml
new file mode 100644
index 0000000000..aeedb5a660
--- /dev/null
+++ b/.github/workflows/publish-autoinstrumentation-e2e-images.yaml
@@ -0,0 +1,61 @@
+name: "Publish instrumentation E2E images"
+
+on:
+  push:
+    paths:
+      - 'tests/instrumentation-e2e-apps/**'
+      - '.github/workflows/publish-autoinstrumentation-e2e-images.yaml'
+    branches:
+      - main
+  pull_request:
+    paths:
+      - 'tests/instrumentation-e2e-apps/**'
+      - '.github/workflows/publish-autoinstrumentation-e2e-images.yaml'
+  workflow_dispatch:
+
+concurrency:
+  group: ${{ github.workflow }}-${{ github.head_ref || github.run_id }}
+  cancel-in-progress: true
+
+jobs:
+  golang:
+    runs-on: ubuntu-20.04
+
+    steps:
+      - uses: actions/checkout@v3
+
+      - name: Docker meta
+        id: meta
+        uses: docker/metadata-action@v4
+        with:
+          images: ghcr.io/open-telemetry/opentelemetry-operator/e2e-test-app-golang
+
+      - name: Set up QEMU
+        uses: docker/setup-qemu-action@v2
+
+      - name: Set up Docker Buildx
+        uses: docker/setup-buildx-action@v2
+
+      - name: Cache Docker layers
+        uses: actions/cache@v3
+        with:
+          path: /tmp/.buildx-cache
+          key: ${{ runner.os }}-buildx-${{ github.sha }}
+          restore-keys: |
+            ${{ runner.os }}-buildx-
+
+      - name: Login to GitHub Package Registry
+        uses: docker/login-action@v2
+        with:
+          registry: ghcr.io
+          username: ${{ github.repository_owner }}
+          password: ${{ secrets.GITHUB_TOKEN }}
+
+      - name: Build and push
+        uses: docker/build-push-action@v4
+        with:
+          context: tests/instrumentation-e2e-apps/golang
+          platforms: linux/arm64,linux/amd64,linux/s390x,linux/ppc64le
+          push: ${{ github.event_name == 'push' }}
+          cache-from: type=local,src=/tmp/.buildx-cache
+          cache-to: type=local,dest=/tmp/.buildx-cache
diff --git a/tests/instrumentation-e2e-apps/golang/Dockerfile b/tests/instrumentation-e2e-apps/golang/Dockerfile
new file mode 100644
index 0000000000..fd80c06f1b
--- /dev/null
+++ b/tests/instrumentation-e2e-apps/golang/Dockerfile
@@ -0,0 +1,8 @@
+FROM golang:1.21-alpine as builder
+
+COPY main.go .
+RUN CGO_ENABLED=0 GOOS=linux GO111MODULE=on go build -o app main.go
+
+FROM scratch
+COPY --from=builder /go/app .
+ENTRYPOINT ["./app"]
diff --git a/tests/instrumentation-e2e-apps/golang/main.go b/tests/instrumentation-e2e-apps/golang/main.go
new file mode 100644
index 0000000000..ad36410372
--- /dev/null
+++ b/tests/instrumentation-e2e-apps/golang/main.go
@@ -0,0 +1,37 @@
+// Copyright The OpenTelemetry Authors
+//
+// Licensed under the Apache License, Version 2.0 (the "License");
+// you may not use this file except in compliance with the License.
+// You may obtain a copy of the License at
+//
+//     http://www.apache.org/licenses/LICENSE-2.0
+//
+// Unless required by applicable law or agreed to in writing, software
+// distributed under the License is distributed on an "AS IS" BASIS,
+// WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+// See the License for the specific language governing permissions and
+// limitations under the License.
+
+package main
+
+import (
+	"fmt"
+	"net/http"
+	"time"
+)
+
+func main() {
+	http.HandleFunc("/", func(w http.ResponseWriter, r *http.Request) {
+		fmt.Println("Hi")
+	})
+
+	server := &http.Server{
+		Addr:              ":8080",
+		ReadHeaderTimeout: 3 * time.Second,
+	}
+
+	err := server.ListenAndServe()
+	if err != nil {
+		panic(err)
+	}
+}