diff --git a/confmap/confmap.go b/confmap/confmap.go
index d589d11aa67..c625b8b82fa 100644
--- a/confmap/confmap.go
+++ b/confmap/confmap.go
@@ -200,7 +200,6 @@ func decodeConfig(m *Conf, result any, errorUnused bool, skipTopLevelUnmarshaler
 			// we unmarshal the embedded structs if present to merge with the result:
 			unmarshalerEmbeddedStructsHookFunc(),
 			zeroSliceHookFunc(),
-			negativeUintHookFunc(),
 		),
 	}
 	decoder, err := mapstructure.NewDecoder(dc)
@@ -499,19 +498,6 @@ func zeroSliceHookFunc() mapstructure.DecodeHookFuncValue {
 	}
 }
 
-// This hook is used to solve the issue: https://github.com/open-telemetry/opentelemetry-collector/issues/9060
-// Decoding should fail when converting a negative integer to any type of unsigned integer. This prevents
-// negative values being decoded as large uint values.
-// TODO: This should be removed as a part of https://github.com/open-telemetry/opentelemetry-collector/issues/9532
-func negativeUintHookFunc() mapstructure.DecodeHookFuncValue {
-	return func(from reflect.Value, to reflect.Value) (interface{}, error) {
-		if from.CanInt() && from.Int() < 0 && to.CanUint() {
-			return nil, fmt.Errorf("cannot convert negative value %v to an unsigned integer", from.Int())
-		}
-		return from.Interface(), nil
-	}
-}
-
 type moduleFactory[T any, S any] interface {
 	Create(s S) T
 }
diff --git a/confmap/confmap_test.go b/confmap/confmap_test.go
index ac48d0359e6..32bf272d5d8 100644
--- a/confmap/confmap_test.go
+++ b/confmap/confmap_test.go
@@ -279,7 +279,7 @@ func TestUintUnmarshalerFailure(t *testing.T) {
 	err := conf.Unmarshal(cfg)
 
 	assert.Error(t, err)
-	assert.Contains(t, err.Error(), fmt.Sprintf("decoding failed due to the following error(s):\n\nerror decoding 'uint_test': cannot convert negative value %v to an unsigned integer", testValue))
+	assert.Contains(t, err.Error(), fmt.Sprintf("decoding failed due to the following error(s):\n\ncannot parse 'uint_test', %d overflows uint", testValue))
 }
 
 func TestMapKeyStringToMapKeyTextUnmarshalerHookFuncDuplicateID(t *testing.T) {
diff --git a/internal/memorylimiter/config_test.go b/internal/memorylimiter/config_test.go
index 032f4798988..2d5a08aebe3 100644
--- a/internal/memorylimiter/config_test.go
+++ b/internal/memorylimiter/config_test.go
@@ -99,6 +99,6 @@ func TestUnmarshalInvalidConfig(t *testing.T) {
 	cfg := &Config{}
 	err = cm.Unmarshal(&cfg)
 	require.Error(t, err)
-	require.Contains(t, err.Error(), "error decoding 'limit_mib': cannot convert negative value -2000 to an unsigned integer")
-	require.Contains(t, err.Error(), "error decoding 'spike_limit_mib': cannot convert negative value -2300 to an unsigned integer")
+	require.Contains(t, err.Error(), "cannot parse 'limit_mib', -2000 overflows uint")
+	require.Contains(t, err.Error(), "cannot parse 'spike_limit_mib', -2300 overflows uint")
 }