diff --git a/lib/internal/fs/promises.js b/lib/internal/fs/promises.js
index c1842fdb4640cd..a035a8b5a0a609 100644
--- a/lib/internal/fs/promises.js
+++ b/lib/internal/fs/promises.js
@@ -539,6 +539,7 @@ async function readFileHandle(filehandle, options) {
     throw new ERR_FS_FILE_TOO_LARGE(size);
 
   let totalRead = 0;
+  const noSize = size === 0;
   let buffer = Buffer.allocUnsafeSlow(length);
   let result = '';
   let offset = 0;
@@ -561,7 +562,7 @@ async function readFileHandle(filehandle, options) {
 
     if (bytesRead === 0 ||
         totalRead === size ||
-        (bytesRead !== buffer.length && !chunkedRead)) {
+        (bytesRead !== buffer.length && !chunkedRead && !noSize)) {
       const singleRead = bytesRead === totalRead;
 
       const bytesToCheck = chunkedRead ? totalRead : bytesRead;
@@ -571,7 +572,7 @@ async function readFileHandle(filehandle, options) {
       }
 
       if (!encoding) {
-        if (size === 0 && !singleRead) {
+        if (noSize && !singleRead) {
           ArrayPrototypePush(buffers, buffer);
           return Buffer.concat(buffers, totalRead);
         }
@@ -584,15 +585,17 @@ async function readFileHandle(filehandle, options) {
       result += decoder.end(buffer);
       return result;
     }
-
+    const readBuffer = bytesRead !== buffer.length ?
+      buffer.subarray(0, bytesRead) :
+      buffer;
     if (encoding) {
-      result += decoder.write(buffer);
+      result += decoder.write(readBuffer);
     } else if (size !== 0) {
       offset = totalRead;
     } else {
       buffers ??= [];
       // Unknown file size requires chunks.
-      ArrayPrototypePush(buffers, buffer);
+      ArrayPrototypePush(buffers, readBuffer);
       buffer = Buffer.allocUnsafeSlow(kReadFileUnknownBufferLength);
     }
   }
diff --git a/test/parallel/test-fs-readfile-eof.js b/test/parallel/test-fs-readfile-eof.js
new file mode 100644
index 00000000000000..94354b915b8dd5
--- /dev/null
+++ b/test/parallel/test-fs-readfile-eof.js
@@ -0,0 +1,46 @@
+'use strict';
+const common = require('../common');
+
+if (common.isWindows || common.isAIX || common.isIBMi)
+  common.skip(`No /dev/stdin on ${process.platform}.`);
+
+const assert = require('assert');
+const fs = require('fs/promises');
+const childType = ['child-encoding', 'child-non-encoding'];
+
+if (process.argv[2] === childType[0]) {
+  fs.readFile('/dev/stdin', 'utf8').then((data) => {
+    process.stdout.write(data);
+  });
+  return;
+} else if (process.argv[2] === childType[1]) {
+  fs.readFile('/dev/stdin').then((data) => {
+    process.stdout.write(data);
+  });
+  return;
+}
+
+const data1 = 'Hello';
+const data2 = 'World';
+const expected = `${data1}\n${data2}\n`;
+
+const exec = require('child_process').exec;
+const f = JSON.stringify(__filename);
+const node = JSON.stringify(process.execPath);
+
+function test(child) {
+  const cmd = `(echo ${data1}; sleep 0.5; echo ${data2}) | ${node} ${f} ${child}`;
+  exec(cmd, common.mustSucceed((stdout, stderr) => {
+    assert.strictEqual(
+      stdout,
+      expected,
+      `expected to read(${child === childType[0] ? 'with' : 'without'} encoding): '${expected}' but got: '${stdout}'`);
+    assert.strictEqual(
+      stderr,
+      '',
+      `expected not to read anything from stderr but got: '${stderr}'`);
+  }));
+}
+
+test(childType[0]);
+test(childType[1]);