diff --git a/test/node-api/test_uv_threadpool_size/node-options.js b/test/node-api/test_uv_threadpool_size/node-options.js
new file mode 100644
index 00000000000000..7f56cea72816b7
--- /dev/null
+++ b/test/node-api/test_uv_threadpool_size/node-options.js
@@ -0,0 +1,33 @@
+'use strict';
+
+const common = require('../../common');
+const assert = require('node:assert');
+const path = require('node:path');
+const { describe, it } = require('node:test');
+
+if (process.config.variables.node_without_node_options) {
+  common.skip('missing NODE_OPTIONS support');
+}
+
+const uvThreadPoolPath = '../../fixtures/dotenv/uv-threadpool.env';
+
+describe('.env supports NODE_OPTIONS', () => {
+
+  it('should update UV_THREADPOOL_SIZE', async () => {
+    const filePath = path.join(__dirname, `./build/${common.buildType}/test_uv_threadpool_size`);
+    const code = `
+      const { test } = require('${filePath}');
+      const size = parseInt(process.env.UV_THREADPOOL_SIZE, 10);
+      require('assert').strictEqual(size, 5);
+      test(size);
+    `.trim();
+    const child = await common.spawnPromisified(
+      process.execPath,
+      [ `--env-file=${uvThreadPoolPath}`, '--eval', code ],
+      { cwd: __dirname },
+    );
+    assert.strictEqual(child.stderr, '');
+    assert.strictEqual(child.code, 0);
+  });
+
+});
diff --git a/test/parallel/test-dotenv-node-options.js b/test/parallel/test-dotenv-node-options.js
index 8a760c6a75b0c4..d0fe177327754d 100644
--- a/test/parallel/test-dotenv-node-options.js
+++ b/test/parallel/test-dotenv-node-options.js
@@ -9,7 +9,6 @@ if (process.config.variables.node_without_node_options) {
 }
 
 const nodeOptionsPath = '../fixtures/dotenv/node-options.env';
-const uvThreadPoolPath = '../fixtures/dotenv/uv-threadpool.env';
 
 describe('.env supports NODE_OPTIONS', () => {
 
@@ -58,19 +57,4 @@ describe('.env supports NODE_OPTIONS', () => {
     assert.strictEqual(child.code, 0);
   });
 
-  it('should update UV_THREADPOOL_SIZE', async () => {
-    const code = `
-      const { test } = require('../node-api/test_uv_threadpool_size/build/${common.buildType}/test_uv_threadpool_size');
-      const size = parseInt(process.env.UV_THREADPOOL_SIZE || 4, 10);
-      test(size);
-    `.trim();
-    const child = await common.spawnPromisified(
-      process.execPath,
-      [ `--env-file=${uvThreadPoolPath}`, '--eval', code ],
-      { cwd: __dirname },
-    );
-    assert.strictEqual(child.stderr, '');
-    assert.strictEqual(child.code, 0);
-  });
-
 });