From e11b3dee2f1ab0d23b1db95ff6098d65e32d88ca Mon Sep 17 00:00:00 2001 From: subwaystation Date: Wed, 21 Jun 2023 14:20:01 +0200 Subject: [PATCH 01/83] update samtools faidx moduels --- modules.json | 2 +- modules/local/extract_communities/main.nf | 6 +++--- modules/nf-core/samtools/faidx/main.nf | 16 +++++++++++----- modules/nf-core/samtools/faidx/meta.yml | 14 ++++++++++++-- 4 files changed, 27 insertions(+), 11 deletions(-) diff --git a/modules.json b/modules.json index 5ddcb646..c0c3a5d8 100644 --- a/modules.json +++ b/modules.json @@ -67,7 +67,7 @@ }, "samtools/faidx": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe", "installed_by": ["modules"] }, "seqwish/induce": { diff --git a/modules/local/extract_communities/main.nf b/modules/local/extract_communities/main.nf index 6bf6ce96..5096b1a9 100644 --- a/modules/local/extract_communities/main.nf +++ b/modules/local/extract_communities/main.nf @@ -2,10 +2,10 @@ process EXTRACT_COMMUNITIES { tag "$meta" label 'process_single' - conda "bioconda::samtools=1.16.1" + conda "bioconda::samtools=1.17" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' : - 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : + 'biocontainers/samtools:1.17--h00cdaf9_0' }" input: tuple val(meta), path(fasta), path(community) diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf index 4dd0e5b0..59ed3088 100644 --- a/modules/nf-core/samtools/faidx/main.nf +++ b/modules/nf-core/samtools/faidx/main.nf @@ -9,11 +9,13 @@ process SAMTOOLS_FAIDX { input: tuple val(meta), path(fasta) + tuple val(meta2), path(fai) output: - tuple val(meta), path ("*.fai"), emit: fai - tuple val(meta), path ("*.gzi"), emit: gzi, optional: true - path "versions.yml" , emit: versions + tuple val(meta), path ("*.{fa,fasta}") , emit: fa , optional: true + tuple val(meta), path ("*.fai") , emit: fai, optional: true + tuple val(meta), path ("*.gzi") , emit: gzi, optional: true + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -23,8 +25,8 @@ process SAMTOOLS_FAIDX { """ samtools \\ faidx \\ - $args \\ - $fasta + $fasta \\ + $args cat <<-END_VERSIONS > versions.yml "${task.process}": @@ -33,8 +35,12 @@ process SAMTOOLS_FAIDX { """ stub: + def match = (task.ext.args =~ /-o(?:utput)?\s(.*)\s?/).findAll() + def fastacmd = match[0] ? "touch ${match[0][1]}" : '' """ + ${fastacmd} touch ${fasta}.fai + cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/samtools/faidx/meta.yml b/modules/nf-core/samtools/faidx/meta.yml index fe2fe9a1..957b25e5 100644 --- a/modules/nf-core/samtools/faidx/meta.yml +++ b/modules/nf-core/samtools/faidx/meta.yml @@ -3,6 +3,7 @@ description: Index FASTA file keywords: - index - fasta + - faidx tools: - samtools: description: | @@ -17,12 +18,21 @@ input: - meta: type: map description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] + Groovy Map containing reference information + e.g. [ id:'test' ] - fasta: type: file description: FASTA file pattern: "*.{fa,fasta}" + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test' ] + - fai: + type: file + description: FASTA index file + pattern: "*.{fai}" output: - meta: type: map From 89f0b140e51e7c1f59fbf0717b890228fb5490ce Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 1 Aug 2023 11:00:58 +0200 Subject: [PATCH 02/83] bump to 1.1.0dev --- CHANGELOG.md | 2 ++ nextflow.config | 2 +- 2 files changed, 3 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ddfef77a..04673be8 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -2,6 +2,8 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). +## 1.1.0dev - Schmuddlweddr + ## 1.0.0 - Ratzupaltuff Initial release of nf-core/pangenome, created with the [nf-core](https://nf-co.re/) template. diff --git a/nextflow.config b/nextflow.config index 17f52e24..6477a877 100644 --- a/nextflow.config +++ b/nextflow.config @@ -270,7 +270,7 @@ manifest { description = """The pangenome graph construction pipeline renders a collection of sequences into a pangenome graph. Its goal is to build a graph that is locally directed and acyclic while preserving large-scale variation. Maintaining local linearity is important for interpretation, visualization, mapping, comparative genomics, and reuse of pangenome graphs""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.0.0' + version = '1.1.0dev' doi = '' } From 48d6a3a569aecc13c765afa42b24b03f6381b3f0 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 1 Aug 2023 11:34:24 +0200 Subject: [PATCH 03/83] try manual fix of multiqc_config --- assets/multiqc_config.yml | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 92379cb9..ea74319d 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/pangenome + This report has been generated by the nf-core/pangenome analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-pangenome-methods-description": order: -1000 From 5375a6b5539937651801e4681a15daafb662749d Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 21 Aug 2023 14:03:04 +0200 Subject: [PATCH 04/83] update gfaffix, multiqc, samtools/faidx --- modules.json | 6 +++--- modules/nf-core/gfaffix/main.nf | 6 +++--- modules/nf-core/multiqc/main.nf | 6 +++--- modules/nf-core/samtools/faidx/main.nf | 16 +++++++++++----- modules/nf-core/samtools/faidx/meta.yml | 14 ++++++++++++-- 5 files changed, 32 insertions(+), 16 deletions(-) diff --git a/modules.json b/modules.json index 9fa43519..82b24aee 100644 --- a/modules.json +++ b/modules.json @@ -12,12 +12,12 @@ }, "gfaffix": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "f1839b597eab2cdcdf05dd8a9419581d76aca197", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "a6e11ac655e744f7ebc724be669dd568ffdc0e80", "installed_by": ["modules"] }, "odgi/build": { @@ -67,7 +67,7 @@ }, "samtools/faidx": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe", "installed_by": ["modules"] }, "seqwish/induce": { diff --git a/modules/nf-core/gfaffix/main.nf b/modules/nf-core/gfaffix/main.nf index 14308f24..64f85bc8 100644 --- a/modules/nf-core/gfaffix/main.nf +++ b/modules/nf-core/gfaffix/main.nf @@ -2,11 +2,11 @@ process GFAFFIX { tag "$meta.id" label 'process_single' - conda "bioconda::gfaffix=0.1.4" + conda "bioconda::gfaffix=0.1.5" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gfaffix:0.1.4--hec16e2b_0' : - 'biocontainers/gfaffix:0.1.4--hec16e2b_0' }" + 'https://depot.galaxyproject.org/singularity/gfaffix:0.1.5--h031d066_0 ' : + 'biocontainers/gfaffix:0.1.5--h031d066_0' }" input: tuple val(meta), path(gfa) diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1fc387be..65d7dd0d 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -1,10 +1,10 @@ process MULTIQC { label 'process_single' - conda "bioconda::multiqc=1.14" + conda "bioconda::multiqc=1.15" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.15--pyhdfd78af_0' : + 'biocontainers/multiqc:1.15--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf index 4dd0e5b0..59ed3088 100644 --- a/modules/nf-core/samtools/faidx/main.nf +++ b/modules/nf-core/samtools/faidx/main.nf @@ -9,11 +9,13 @@ process SAMTOOLS_FAIDX { input: tuple val(meta), path(fasta) + tuple val(meta2), path(fai) output: - tuple val(meta), path ("*.fai"), emit: fai - tuple val(meta), path ("*.gzi"), emit: gzi, optional: true - path "versions.yml" , emit: versions + tuple val(meta), path ("*.{fa,fasta}") , emit: fa , optional: true + tuple val(meta), path ("*.fai") , emit: fai, optional: true + tuple val(meta), path ("*.gzi") , emit: gzi, optional: true + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -23,8 +25,8 @@ process SAMTOOLS_FAIDX { """ samtools \\ faidx \\ - $args \\ - $fasta + $fasta \\ + $args cat <<-END_VERSIONS > versions.yml "${task.process}": @@ -33,8 +35,12 @@ process SAMTOOLS_FAIDX { """ stub: + def match = (task.ext.args =~ /-o(?:utput)?\s(.*)\s?/).findAll() + def fastacmd = match[0] ? "touch ${match[0][1]}" : '' """ + ${fastacmd} touch ${fasta}.fai + cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/samtools/faidx/meta.yml b/modules/nf-core/samtools/faidx/meta.yml index fe2fe9a1..957b25e5 100644 --- a/modules/nf-core/samtools/faidx/meta.yml +++ b/modules/nf-core/samtools/faidx/meta.yml @@ -3,6 +3,7 @@ description: Index FASTA file keywords: - index - fasta + - faidx tools: - samtools: description: | @@ -17,12 +18,21 @@ input: - meta: type: map description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] + Groovy Map containing reference information + e.g. [ id:'test' ] - fasta: type: file description: FASTA file pattern: "*.{fa,fasta}" + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test' ] + - fai: + type: file + description: FASTA index file + pattern: "*.{fai}" output: - meta: type: map From c28f08dba890f1002b88eeddd31e8eb0c5513604 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 21 Aug 2023 14:10:37 +0200 Subject: [PATCH 05/83] try to fix samtools/faidx --- subworkflows/local/community.nf | 2 +- subworkflows/local/input_check.nf | 4 ++-- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/subworkflows/local/community.nf b/subworkflows/local/community.nf index a843a1b9..e292e110 100644 --- a/subworkflows/local/community.nf +++ b/subworkflows/local/community.nf @@ -45,7 +45,7 @@ workflow COMMUNITY { TABIX_BGZIP(EXTRACT_COMMUNITIES.out.community_fasta) ch_versions = ch_versions.mix(TABIX_BGZIP.out.versions) - SAMTOOLS_FAIDX(TABIX_BGZIP.out.output) + SAMTOOLS_FAIDX(TABIX_BGZIP.out.output, [[],[]]) ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions) emit: diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 6be6c7a1..a0cfeb56 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -28,7 +28,7 @@ workflow INPUT_CHECK { // TODO We want to check, if the input file was actually compressed with bgzip with the upcoming grabix module. // For now we assume it was bgzip. If not WFMASH will complain instantly anyhow. if (!fai_path.exists() || !gzi_path.exists()) { // the assumption is that none of these files exist if only one does not exist - SAMTOOLS_FAIDX(meta_fasta) + SAMTOOLS_FAIDX(meta_fasta, [[],[]]) fai = SAMTOOLS_FAIDX.out.fai gzi = SAMTOOLS_FAIDX.out.gzi ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions) @@ -50,7 +50,7 @@ workflow INPUT_CHECK { meta_fasta = tuple([ id:fasta_file_name ], fasta) TABIX_BGZIP(meta_fasta) ch_fasta = TABIX_BGZIP.out.output - SAMTOOLS_FAIDX(ch_fasta) + SAMTOOLS_FAIDX(ch_fasta, [[],[]]) gzi = SAMTOOLS_FAIDX.out.gzi fai = SAMTOOLS_FAIDX.out.fai ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions) From 165121007396e436f388dbced291b0cedee01ecc Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 21 Aug 2023 15:32:31 +0200 Subject: [PATCH 06/83] vg deconstruct 1.50.1 --- modules/local/multiqc_community/main.nf | 6 +++--- modules/local/paf2net/main.nf | 6 +++--- modules/local/vg_deconstruct/main.nf | 2 +- 3 files changed, 7 insertions(+), 7 deletions(-) diff --git a/modules/local/multiqc_community/main.nf b/modules/local/multiqc_community/main.nf index efa169fe..80447816 100644 --- a/modules/local/multiqc_community/main.nf +++ b/modules/local/multiqc_community/main.nf @@ -2,10 +2,10 @@ process MULTIQC_COMMUNITY { tag "$meta.id" label 'process_single' - conda "bioconda::multiqc=1.14" + conda "bioconda::multiqc=1.15" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.15--pyhdfd78af_0' : + 'biocontainers/multiqc:1.15--pyhdfd78af_0' }" input: tuple val(meta), path(multiqc_files, stageAs: "?/*") diff --git a/modules/local/paf2net/main.nf b/modules/local/paf2net/main.nf index 97644008..5e744e88 100644 --- a/modules/local/paf2net/main.nf +++ b/modules/local/paf2net/main.nf @@ -2,10 +2,10 @@ process PAF2NET { tag "$meta.id" label 'process_single' - conda "bioconda::pggb=0.5.3" + conda "bioconda::pggb=0.5.4" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/pggb:0.5.3--hdfd78af_2': - 'biocontainers/pggb:0.5.3--hdfd78af_2' }" + 'https://depot.galaxyproject.org/singularity/pggb:0.5.4--hdfd78af_0': + 'biocontainers/pggb:0.5.4--hdfd78af_0' }" input: tuple val(meta), path(paf) diff --git a/modules/local/vg_deconstruct/main.nf b/modules/local/vg_deconstruct/main.nf index c727cdc8..ece466eb 100644 --- a/modules/local/vg_deconstruct/main.nf +++ b/modules/local/vg_deconstruct/main.nf @@ -29,7 +29,7 @@ process VG_DECONSTRUCT { 'https://depot.galaxyproject.org/singularity/pggb:0.5.3--hdfd78af_2': 'quay.io/biocontainers/pggb:0.5.3--hdfd78af_2' }" */ - container "ghcr.io/pangenome/pggb:2023062221192542ea28" + container "ghcr.io/pangenome/pggb:20230819064109936a2c" input: tuple val(meta), path(graph), val(vcf_spec) From 63707cb5be21bad29a8d4ab67397fbdda466581d Mon Sep 17 00:00:00 2001 From: subwaystation Date: Wed, 23 Aug 2023 09:28:43 +0200 Subject: [PATCH 07/83] update smoothxg to v0.7.1 --- modules.json | 4 ++-- modules/nf-core/custom/dumpsoftwareversions/main.nf | 6 +++--- modules/nf-core/smoothxg/main.nf | 6 +++--- 3 files changed, 8 insertions(+), 8 deletions(-) diff --git a/modules.json b/modules.json index 82b24aee..ca18543b 100644 --- a/modules.json +++ b/modules.json @@ -7,7 +7,7 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "05c280924b6c768d484c7c443dad5e605c4ff4b4", "installed_by": ["modules"] }, "gfaffix": { @@ -77,7 +77,7 @@ }, "smoothxg": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "d7c40cb04c94c408608ceabfdc14a443915a87fe", "installed_by": ["modules"] }, "tabix/bgzip": { diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index ebc87273..c9d014b1 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "bioconda::multiqc=1.14" + conda "bioconda::multiqc=1.15" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.15--pyhdfd78af_0' : + 'biocontainers/multiqc:1.15--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/smoothxg/main.nf b/modules/nf-core/smoothxg/main.nf index 8e95bd1d..0d10a4a4 100644 --- a/modules/nf-core/smoothxg/main.nf +++ b/modules/nf-core/smoothxg/main.nf @@ -2,10 +2,10 @@ process SMOOTHXG { tag "$meta.id" label 'process_high' - conda "bioconda::smoothxg=0.7.0" + conda "bioconda::smoothxg=0.7.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/smoothxg:0.7.0--hfb1f815_0' : - 'biocontainers/smoothxg:0.7.0--hfb1f815_0' }" + 'https://depot.galaxyproject.org/singularity/smoothxg:0.7.1--h40c17d1_0' : + 'biocontainers/smoothxg:0.7.1--h40c17d1_0' }" input: tuple val(meta), path(gfa) From d1f0eecc57db9e79ab91ceef8421616984df181f Mon Sep 17 00:00:00 2001 From: subwaystation Date: Wed, 23 Aug 2023 10:08:08 +0200 Subject: [PATCH 08/83] also put DRAW into MultiQC report --- assets/multiqc_config.yml | 2 -- workflows/pangenome.nf | 2 +- 2 files changed, 1 insertion(+), 3 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index ea74319d..bb584caa 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -52,8 +52,6 @@ sp: fn: "*viz_depth_multiqc.png" odgi_viz_uncalled: fn: "*viz_uncalled_multiqc.png" - testing_name: - fn: "*draw.png" ignore_images: false # Make the custom content stuff come after the ODGI module output diff --git a/workflows/pangenome.nf b/workflows/pangenome.nf index 2d61542f..df87b3c4 100644 --- a/workflows/pangenome.nf +++ b/workflows/pangenome.nf @@ -159,7 +159,7 @@ workflow PANGENOME { ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) if (!params.communities) { if (!params.wfmash_only) { - ch_multiqc_files = ch_multiqc_files.mix(PGGB.out.qc.map{return it[1..8]}) + ch_multiqc_files = ch_multiqc_files.mix(PGGB.out.qc.map{return it[1..9]}) } } else { ch_multiqc_files = ch_multiqc_files.mix(ODGI_QC.out.qc.map{return it[1..8]}) From f69a7f97794ff5958e65fdb958968df8c3a81275 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Wed, 23 Aug 2023 16:06:52 +0200 Subject: [PATCH 09/83] another MultiQC fix --- subworkflows/local/odgi_qc.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/odgi_qc.nf b/subworkflows/local/odgi_qc.nf index 8b7a8b84..967f5111 100644 --- a/subworkflows/local/odgi_qc.nf +++ b/subworkflows/local/odgi_qc.nf @@ -63,7 +63,7 @@ workflow ODGI_QC { ch_graph_qc = ch_graph_qc.join(ODGI_VIZ_UNCALLED.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".gfaffix.*", "") ], png ]}) ch_graph_qc = ch_graph_qc.join(ODGI_DRAW_MULTIQC.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".gfaffix.*", "") ], png ]}) } else { - ch_graph_qc = ODGI_STATS.out.yaml.map{meta, yaml -> [ [ id: meta.id.replaceFirst(".seqwish", ".gfaffix") ], yaml ]}.groupTuple(by:0, size:2).map{meta, yamls -> [ meta, yamls[0], yamls[1] ]} + ch_graph_qc = ODGI_STATS.out.yaml ch_graph_qc = ch_graph_qc.join(ODGI_VIZ_COLOR.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".viz", "") ], png ]}) ch_graph_qc = ch_graph_qc.join(ODGI_VIZ_POS.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".viz_pos", "") ], png ]}) ch_graph_qc = ch_graph_qc.join(ODGI_VIZ_DEPTH.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".viz_depth", "") ], png ]}) From 9eafdf30e97ba81738e0c3f5ecee444ef87ec15a Mon Sep 17 00:00:00 2001 From: subwaystation Date: Wed, 23 Aug 2023 16:13:56 +0200 Subject: [PATCH 10/83] life is strange --- workflows/pangenome.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/pangenome.nf b/workflows/pangenome.nf index df87b3c4..2d61542f 100644 --- a/workflows/pangenome.nf +++ b/workflows/pangenome.nf @@ -159,7 +159,7 @@ workflow PANGENOME { ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) if (!params.communities) { if (!params.wfmash_only) { - ch_multiqc_files = ch_multiqc_files.mix(PGGB.out.qc.map{return it[1..9]}) + ch_multiqc_files = ch_multiqc_files.mix(PGGB.out.qc.map{return it[1..8]}) } } else { ch_multiqc_files = ch_multiqc_files.mix(ODGI_QC.out.qc.map{return it[1..8]}) From 1b545e9e545e562f2d87544ff0931f6692cbcf8e Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 4 Sep 2023 13:21:39 +0200 Subject: [PATCH 11/83] update odgi and wfmash --- modules.json | 20 ++++++++++---------- modules/nf-core/odgi/build/main.nf | 6 +++--- modules/nf-core/odgi/draw/main.nf | 6 +++--- modules/nf-core/odgi/layout/main.nf | 6 +++--- modules/nf-core/odgi/sort/main.nf | 6 +++--- modules/nf-core/odgi/squeeze/main.nf | 6 +++--- modules/nf-core/odgi/squeeze/meta.yml | 2 +- modules/nf-core/odgi/stats/main.nf | 6 +++--- modules/nf-core/odgi/unchop/main.nf | 6 +++--- modules/nf-core/odgi/view/main.nf | 6 +++--- modules/nf-core/odgi/viz/main.nf | 6 +++--- modules/nf-core/wfmash/main.nf | 6 +++--- 12 files changed, 41 insertions(+), 41 deletions(-) diff --git a/modules.json b/modules.json index ca18543b..4a3b6248 100644 --- a/modules.json +++ b/modules.json @@ -22,47 +22,47 @@ }, "odgi/build": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", "installed_by": ["modules"] }, "odgi/draw": { "branch": "master", - "git_sha": "b986764032c2bb042a71b4f503f8fafe3695bd39", + "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", "installed_by": ["modules"] }, "odgi/layout": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", "installed_by": ["modules"] }, "odgi/sort": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", "installed_by": ["modules"] }, "odgi/squeeze": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", "installed_by": ["modules"] }, "odgi/stats": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", "installed_by": ["modules"] }, "odgi/unchop": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", "installed_by": ["modules"] }, "odgi/view": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", "installed_by": ["modules"] }, "odgi/viz": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", "installed_by": ["modules"] }, "samtools/faidx": { @@ -87,7 +87,7 @@ }, "wfmash": { "branch": "master", - "git_sha": "77c0c76b7eb7ec564617e635953addc9fb40b61e", + "git_sha": "96fadda088c6e79777ba8eef939e36bb03a69c65", "installed_by": ["modules"] } } diff --git a/modules/nf-core/odgi/build/main.nf b/modules/nf-core/odgi/build/main.nf index 6e7f20e7..938c16bb 100644 --- a/modules/nf-core/odgi/build/main.nf +++ b/modules/nf-core/odgi/build/main.nf @@ -2,10 +2,10 @@ process ODGI_BUILD { tag "$meta.id" label 'process_medium' - conda "bioconda::odgi=0.8.2" + conda "bioconda::odgi=0.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.2--py310hc8f18ef_0': - 'biocontainers/odgi:0.8.2--py310hc8f18ef_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': + 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" input: tuple val(meta), path(graph) diff --git a/modules/nf-core/odgi/draw/main.nf b/modules/nf-core/odgi/draw/main.nf index 61ad0826..426c258e 100644 --- a/modules/nf-core/odgi/draw/main.nf +++ b/modules/nf-core/odgi/draw/main.nf @@ -2,10 +2,10 @@ process ODGI_DRAW { tag "$meta.id" label 'process_single' - conda "bioconda::odgi=0.8.2" + conda "bioconda::odgi=0.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.2--py310hc8f18ef_0': - 'biocontainers/odgi:0.8.2--py310hc8f18ef_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': + 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" input: tuple val(meta), path(graph), path(lay) diff --git a/modules/nf-core/odgi/layout/main.nf b/modules/nf-core/odgi/layout/main.nf index 2edfda2d..22e7c438 100644 --- a/modules/nf-core/odgi/layout/main.nf +++ b/modules/nf-core/odgi/layout/main.nf @@ -2,10 +2,10 @@ process ODGI_LAYOUT { tag "$meta.id" label 'process_medium' - conda "bioconda::odgi=0.8.2" + conda "bioconda::odgi=0.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.2--py310hc8f18ef_0': - 'biocontainers/odgi:0.8.2--py310hc8f18ef_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': + 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" input: tuple val(meta), path(graph) diff --git a/modules/nf-core/odgi/sort/main.nf b/modules/nf-core/odgi/sort/main.nf index 9b6ac30f..d57cc04a 100644 --- a/modules/nf-core/odgi/sort/main.nf +++ b/modules/nf-core/odgi/sort/main.nf @@ -2,10 +2,10 @@ process ODGI_SORT { tag "$meta.id" label 'process_high' - conda "bioconda::odgi=0.8.2" + conda "bioconda::odgi=0.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.2--py310hc8f18ef_0': - 'biocontainers/odgi:0.8.2--py310hc8f18ef_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': + 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" input: tuple val(meta), path(graph) diff --git a/modules/nf-core/odgi/squeeze/main.nf b/modules/nf-core/odgi/squeeze/main.nf index 3b31df88..a2e187fe 100644 --- a/modules/nf-core/odgi/squeeze/main.nf +++ b/modules/nf-core/odgi/squeeze/main.nf @@ -2,10 +2,10 @@ process ODGI_SQUEEZE { tag "$meta.id" label 'process_medium' - conda "bioconda::odgi=0.8.2" + conda "bioconda::odgi=0.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.2--py310hc8f18ef_0': - 'biocontainers/odgi:0.8.2--py310hc8f18ef_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': + 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" input: tuple val(meta), path(graphs) diff --git a/modules/nf-core/odgi/squeeze/meta.yml b/modules/nf-core/odgi/squeeze/meta.yml index 89acd892..4b6181d3 100644 --- a/modules/nf-core/odgi/squeeze/meta.yml +++ b/modules/nf-core/odgi/squeeze/meta.yml @@ -21,7 +21,7 @@ input: Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - graphs: - type: files + type: file description: Pangenome graph files in ODGI format. pattern: "*.{og}" diff --git a/modules/nf-core/odgi/stats/main.nf b/modules/nf-core/odgi/stats/main.nf index 7600ae1a..99409f65 100644 --- a/modules/nf-core/odgi/stats/main.nf +++ b/modules/nf-core/odgi/stats/main.nf @@ -2,10 +2,10 @@ process ODGI_STATS { tag "$meta.id" label 'process_single' - conda "bioconda::odgi=0.8.2" + conda "bioconda::odgi=0.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.2--py310hc8f18ef_0': - 'biocontainers/odgi:0.8.2--py310hc8f18ef_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': + 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" input: tuple val(meta), path(graph) diff --git a/modules/nf-core/odgi/unchop/main.nf b/modules/nf-core/odgi/unchop/main.nf index a55d99d7..50cbfde2 100644 --- a/modules/nf-core/odgi/unchop/main.nf +++ b/modules/nf-core/odgi/unchop/main.nf @@ -2,10 +2,10 @@ process ODGI_UNCHOP { tag "$meta.id" label 'process_medium' - conda "bioconda::odgi=0.8.2" + conda "bioconda::odgi=0.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.2--py310hc8f18ef_0': - 'biocontainers/odgi:0.8.2--py310hc8f18ef_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': + 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" input: tuple val(meta), path(graph) diff --git a/modules/nf-core/odgi/view/main.nf b/modules/nf-core/odgi/view/main.nf index 4c1de756..cecfe454 100644 --- a/modules/nf-core/odgi/view/main.nf +++ b/modules/nf-core/odgi/view/main.nf @@ -2,10 +2,10 @@ process ODGI_VIEW { tag "$meta.id" label 'process_single' - conda "bioconda::odgi=0.8.2" + conda "bioconda::odgi=0.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.2--py310hc8f18ef_0': - 'biocontainers/odgi:0.8.2--py310hc8f18ef_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': + 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" input: tuple val(meta), path(graph) diff --git a/modules/nf-core/odgi/viz/main.nf b/modules/nf-core/odgi/viz/main.nf index 81da80f1..feef5be2 100644 --- a/modules/nf-core/odgi/viz/main.nf +++ b/modules/nf-core/odgi/viz/main.nf @@ -2,10 +2,10 @@ process ODGI_VIZ { tag "$meta.id" label 'process_single' - conda "bioconda::odgi=0.8.2" + conda "bioconda::odgi=0.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.2--py39h2add14b_0': - 'biocontainers/odgi:0.8.2--py310hc8f18ef_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': + 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" input: tuple val(meta), path(graph) diff --git a/modules/nf-core/wfmash/main.nf b/modules/nf-core/wfmash/main.nf index 33c556ad..0f9c5818 100644 --- a/modules/nf-core/wfmash/main.nf +++ b/modules/nf-core/wfmash/main.nf @@ -2,10 +2,10 @@ process WFMASH { tag "$meta.id" label 'process_medium' - conda "bioconda::wfmash=0.10.4" + conda "bioconda::wfmash=0.10.5" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/wfmash:0.10.4--hea8008d_0': - 'biocontainers/wfmash:0.10.4--hea8008d_0' }" + 'https://depot.galaxyproject.org/singularity/wfmash:0.10.5--h94f6cfe_0': + 'biocontainers/wfmash:0.10.5--h94f6cfe_0' }" input: tuple val(meta), path(fasta_gz), path(paf), path(gzi), path(fai) From a1f80720a66b201f7bcf5f78bad4826120a336cd Mon Sep 17 00:00:00 2001 From: subwaystation Date: Thu, 21 Sep 2023 09:33:46 +0200 Subject: [PATCH 12/83] fix wfmash_sparse_map parameter --- nextflow_schema.json | 21 ++++++++++----------- 1 file changed, 10 insertions(+), 11 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 44f6c3fc..af86f805 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -81,9 +81,16 @@ "description": "Ignore the top % most-frequent kmers." }, "wfmash_sparse_map": { - "type": "number", - "default": "1.0", - "description": "Keep this fraction of mappings (\"auto\" for giant component heuristic)." + "default": 1, + "description": "Keep this fraction of mappings (\"auto\" for giant component heuristic).", + "anyOf": [ + { + "type": "string" + }, + { + "type": "number" + } + ] }, "wfmash_merge_segments": { "type": "boolean", @@ -360,14 +367,12 @@ "type": "boolean", "description": "Display help text.", "fa_icon": "fas fa-question-circle", - "default": false, "hidden": true }, "version": { "type": "boolean", "description": "Display version and exit.", "fa_icon": "fas fa-question-circle", - "default": false, "hidden": true }, "publish_dir_mode": { @@ -391,7 +396,6 @@ "type": "boolean", "description": "Send plain-text email instead of HTML.", "fa_icon": "fas fa-remove-format", - "default": false, "hidden": true }, "max_multiqc_email_size": { @@ -406,7 +410,6 @@ "type": "boolean", "description": "Do not use coloured log outputs.", "fa_icon": "fas fa-palette", - "default": false, "hidden": true }, "hook_url": { @@ -445,7 +448,6 @@ "type": "boolean", "fa_icon": "far fa-eye-slash", "description": "Show all params when using `--help`", - "default": false, "hidden": true, "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." }, @@ -453,7 +455,6 @@ "type": "boolean", "fa_icon": "far fa-check-circle", "description": "Validation of parameters fails when an unrecognised parameter is found.", - "default": false, "hidden": true, "help_text": "By default, when an unrecognised parameter is found, it returns a warinig." }, @@ -461,7 +462,6 @@ "type": "boolean", "fa_icon": "far fa-check-circle", "description": "Validation of parameters in lenient more.", - "default": false, "hidden": true, "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." }, @@ -469,7 +469,6 @@ "type": "boolean", "fa_icon": "far fa-check-circle", "description": "Do we want to display hidden parameters?", - "default": false, "hidden": true, "help_text": "Do we want to display hidden parameters?" }, From 16bcbcc34ef5305eca4525997146ced0fc91e93f Mon Sep 17 00:00:00 2001 From: subwaystation Date: Thu, 21 Sep 2023 09:38:12 +0200 Subject: [PATCH 13/83] add wfmash_sparse_map test --- .github/workflows/ci.yml | 23 +++++++++++++++++++++++ 1 file changed, 23 insertions(+) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index efc70c10..ab887118 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -128,6 +128,29 @@ jobs: run: | nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results ${{ matrix.parameters }} + wfmash_sparse_map: + name: Run pipeline with wfmash_sparse_map parameters + # Only run on push if this is the nf-core dev branch (merged PRs) + if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/pangenome') }}" + runs-on: ubuntu-latest + strategy: + matrix: + parameters: + - "--wfmash_sparse_map 'auto'" + - "--wfmash_sparse_map 0.5" + steps: + - name: Check out pipeline code + uses: actions/checkout@v3 + + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + with: + version: "23.04.0" + + - name: Run pipeline with test data and wfmash_only parameters + run: | + nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results ${{ matrix.parameters }} + communities: name: Run pipeline with community parameters # Only run on push if this is the nf-core dev branch (merged PRs) From 6538720992a57590593a7da84eb68475cc4b26b3 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Thu, 21 Sep 2023 09:45:01 +0200 Subject: [PATCH 14/83] try linter fix --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index af86f805..6fae220e 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -81,7 +81,7 @@ "description": "Ignore the top % most-frequent kmers." }, "wfmash_sparse_map": { - "default": 1, + "default": 1.0, "description": "Keep this fraction of mappings (\"auto\" for giant component heuristic).", "anyOf": [ { From dc5495a5fcb05c0826582b39382c0c411e8e1ca9 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Thu, 21 Sep 2023 09:59:19 +0200 Subject: [PATCH 15/83] try fix --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index a36f5e9c..d6cd51c7 100644 --- a/nextflow.config +++ b/nextflow.config @@ -101,7 +101,7 @@ params { // Schema validation default options validationFailUnrecognisedParams = false - validationLenientMode = false + validationLenientMode = true validationSchemaIgnoreParams = 'genomes' validationShowHiddenParams = false validate_params = true From 290438539759bfcac3015903eacb73b2e4bcc972 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Thu, 21 Sep 2023 10:01:42 +0200 Subject: [PATCH 16/83] add wfmash_sparse_map to ignored params --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index d6cd51c7..f510fc13 100644 --- a/nextflow.config +++ b/nextflow.config @@ -102,7 +102,7 @@ params { // Schema validation default options validationFailUnrecognisedParams = false validationLenientMode = true - validationSchemaIgnoreParams = 'genomes' + validationSchemaIgnoreParams = 'genomes, wfmash_sparse_map' validationShowHiddenParams = false validate_params = true From 2570fd5777eb90dc5415c66b02f84dec0838fc8a Mon Sep 17 00:00:00 2001 From: subwaystation Date: Thu, 21 Sep 2023 10:05:18 +0200 Subject: [PATCH 17/83] revert to string theory --- nextflow_schema.json | 15 ++++----------- 1 file changed, 4 insertions(+), 11 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 6fae220e..58469fb5 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -54,7 +54,7 @@ "properties": { "wfmash_map_pct_id": { "type": "number", - "default": 90, + "default": 90.0, "description": "Percent identity in the wfmash mashmap step.", "help_text": "Use `mash dist` or `mash triangle` to explore the typical level of divergence between the sequences in your input (see https://pggb.readthedocs.io/en/latest/rst/tutorials/divergence_estimation.html#divergence-estimation for more information). Convert this to an approximate percent identity and provide it as --wfmash_map_pct_id . A list of examples can be found at https://github.com/pangenome/pggb#example-builds-for-diverse-species." }, @@ -81,16 +81,9 @@ "description": "Ignore the top % most-frequent kmers." }, "wfmash_sparse_map": { - "default": 1.0, + "default": "1", "description": "Keep this fraction of mappings (\"auto\" for giant component heuristic).", - "anyOf": [ - { - "type": "string" - }, - { - "type": "number" - } - ] + "type": "string" }, "wfmash_merge_segments": { "type": "boolean", @@ -143,7 +136,7 @@ }, "seqwish_sparse_factor": { "type": "number", - "default": 0, + "default": 0.0, "description": "Keep this randomly selected fraction of input matches." }, "seqwish_temp_dir": { From a2a4fe4fb79a3b83ead4779a2b8388dd857c714d Mon Sep 17 00:00:00 2001 From: subwaystation Date: Thu, 21 Sep 2023 10:05:56 +0200 Subject: [PATCH 18/83] remove ignore parameter --wfmash_sparse_map --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index f510fc13..d6cd51c7 100644 --- a/nextflow.config +++ b/nextflow.config @@ -102,7 +102,7 @@ params { // Schema validation default options validationFailUnrecognisedParams = false validationLenientMode = true - validationSchemaIgnoreParams = 'genomes, wfmash_sparse_map' + validationSchemaIgnoreParams = 'genomes' validationShowHiddenParams = false validate_params = true From 7575ba1957791fed4e9cc7268b2571a1517b6c89 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Thu, 21 Sep 2023 20:55:16 +0200 Subject: [PATCH 19/83] update tests, add pattern --- .github/workflows/ci.yml | 2 +- nextflow_schema.json | 3 ++- 2 files changed, 3 insertions(+), 2 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index ab887118..fa3a2c2a 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -136,7 +136,7 @@ jobs: strategy: matrix: parameters: - - "--wfmash_sparse_map 'auto'" + - "--wfmash_sparse_map auto" - "--wfmash_sparse_map 0.5" steps: - name: Check out pipeline code diff --git a/nextflow_schema.json b/nextflow_schema.json index 58469fb5..92b6d53d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -83,7 +83,8 @@ "wfmash_sparse_map": { "default": "1", "description": "Keep this fraction of mappings (\"auto\" for giant component heuristic).", - "type": "string" + "type": "string", + "pattern": "(auto|[01]\\.\\d+)" }, "wfmash_merge_segments": { "type": "boolean", From 90ebc5d8bf3f9e066316e8ecf1e4b5e017fa5d94 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Thu, 21 Sep 2023 21:07:32 +0200 Subject: [PATCH 20/83] update CI, fix linting error --- conf/modules.config | 8 ++++---- nextflow_schema.json | 2 +- 2 files changed, 5 insertions(+), 5 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index f2846ae3..9cb71e57 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -15,10 +15,10 @@ def generate_wfmash_sparse_map_cmd() { def wfmash_sparse_map_cmd = "" if (params.wfmash_sparse_map == "auto") { def n = params.n_haplotypes - def x = Math.log(n)/n * 10 - wfmash_sparse_map_frac = 1 - if (x < 1) { - def wfmash_sparse_map_frac = x + def x = Math.log(n)/n * 10.0 + wfmash_sparse_map_frac = 1.0 + if (x < 1.0) { + wfmash_sparse_map_frac = x } wfmash_sparse_map_cmd = "-x ${wfmash_sparse_map_frac}" } else { diff --git a/nextflow_schema.json b/nextflow_schema.json index 92b6d53d..ce320e94 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -81,7 +81,7 @@ "description": "Ignore the top % most-frequent kmers." }, "wfmash_sparse_map": { - "default": "1", + "default": 1.0, "description": "Keep this fraction of mappings (\"auto\" for giant component heuristic).", "type": "string", "pattern": "(auto|[01]\\.\\d+)" From b271144b1be60578aeb47623b255ea008562a837 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 25 Sep 2023 15:16:17 +0000 Subject: [PATCH 21/83] Template update for nf-core/tools version 2.10 --- .devcontainer/devcontainer.json | 1 + .github/CONTRIBUTING.md | 4 +- .github/workflows/linting.yml | 2 +- .github/workflows/release-announcments.yml | 68 +++++++++ CHANGELOG.md | 2 +- CITATIONS.md | 2 +- CODE_OF_CONDUCT.md | 133 ++++++++++++++---- README.md | 21 +-- assets/multiqc_config.yml | 4 +- conf/modules.config | 9 ++ docs/output.md | 5 +- docs/usage.md | 16 ++- lib/NfcoreTemplate.groovy | 16 +++ lib/WorkflowPangenome.groovy | 2 +- main.nf | 3 + modules.json | 6 +- .../custom/dumpsoftwareversions/main.nf | 2 +- modules/nf-core/fastqc/main.nf | 8 +- modules/nf-core/multiqc/main.nf | 2 +- nextflow.config | 9 +- nextflow_schema.json | 15 -- workflows/pangenome.nf | 1 + 22 files changed, 253 insertions(+), 78 deletions(-) create mode 100644 .github/workflows/release-announcments.yml diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index ea27a584..4ecfbfe3 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -2,6 +2,7 @@ "name": "nfcore", "image": "nfcore/gitpod:latest", "remoteUser": "gitpod", + "runArgs": ["--privileged"], // Configure tool-specific properties. "customizations": { diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 205daa91..2dfb02e4 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,7 +9,9 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -> If you need help using or modifying nf-core/pangenome then the best place to ask is on the nf-core Slack [#pangenome](https://nfcore.slack.com/channels/pangenome) channel ([join our Slack here](https://nf-co.re/join/slack)). +:::info +If you need help using or modifying nf-core/pangenome then the best place to ask is on the nf-core Slack [#pangenome](https://nfcore.slack.com/channels/pangenome) channel ([join our Slack here](https://nf-co.re/join/slack)). +::: ## Contribution workflow diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 888cb4bc..b8bdd214 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -78,7 +78,7 @@ jobs: - uses: actions/setup-python@v4 with: - python-version: "3.8" + python-version: "3.11" architecture: "x64" - name: Install dependencies diff --git a/.github/workflows/release-announcments.yml b/.github/workflows/release-announcments.yml new file mode 100644 index 00000000..6ad33927 --- /dev/null +++ b/.github/workflows/release-announcments.yml @@ -0,0 +1,68 @@ +name: release-announcements +# Automatic release toot and tweet anouncements +on: + release: + types: [published] + workflow_dispatch: + +jobs: + toot: + runs-on: ubuntu-latest + steps: + - uses: rzr/fediverse-action@master + with: + access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} + host: "mstdn.science" # custom host if not "mastodon.social" (default) + # GitHub event payload + # https://docs.github.com/en/developers/webhooks-and-events/webhooks/webhook-events-and-payloads#release + message: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + + send-tweet: + runs-on: ubuntu-latest + + steps: + - uses: actions/setup-python@v4 + with: + python-version: "3.10" + - name: Install dependencies + run: pip install tweepy==4.14.0 + - name: Send tweet + shell: python + run: | + import os + import tweepy + + client = tweepy.Client( + access_token=os.getenv("TWITTER_ACCESS_TOKEN"), + access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"), + consumer_key=os.getenv("TWITTER_CONSUMER_KEY"), + consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"), + ) + tweet = os.getenv("TWEET") + client.create_tweet(text=tweet) + env: + TWEET: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }} + TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }} + TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }} + TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }} + + bsky-post: + runs-on: ubuntu-latest + steps: + - uses: zentered/bluesky-post-action@v0.0.2 + with: + post: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + env: + BSKY_IDENTIFIER: ${{ secrets.BSKY_IDENTIFIER }} + BSKY_PASSWORD: ${{ secrets.BSKY_PASSWORD }} + # diff --git a/CHANGELOG.md b/CHANGELOG.md index 214d9729..77849c44 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.0.0 - [date] +## v1.1.0dev - [date] Initial release of nf-core/pangenome, created with the [nf-core](https://nf-co.re/) template. diff --git a/CITATIONS.md b/CITATIONS.md index 1b818be2..7abae2d6 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -12,7 +12,7 @@ - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) - > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. Available online https://www.bioinformatics.babraham.ac.uk/projects/fastqc/. + > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md index f4fd052f..c089ec78 100644 --- a/CODE_OF_CONDUCT.md +++ b/CODE_OF_CONDUCT.md @@ -1,18 +1,20 @@ -# Code of Conduct at nf-core (v1.0) +# Code of Conduct at nf-core (v1.4) ## Our Pledge -In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: +In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: - Age +- Ability - Body size +- Caste - Familial status - Gender identity and expression - Geographical location - Level of experience - Nationality and national origins - Native language -- Physical and neurological ability +- Neurodiversity - Race or ethnicity - Religion - Sexual identity and orientation @@ -22,80 +24,133 @@ Please note that the list above is alphabetised and is therefore not ranked in a ## Preamble -> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply. +:::note +This Code of Conduct (CoC) has been drafted by Renuka Kudva, Cris Tuñí, and Michael Heuer, with input from the nf-core Core Team and Susanna Marquez from the nf-core community. "We", in this document, refers to the Safety Officers and members of the nf-core Core Team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will be amended periodically to keep it up-to-date. In case of any dispute, the most current version will apply. +::: -An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva. +An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). + +Our Safety Officers are Saba Nafees, Cris Tuñí, and Michael Heuer. nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals. -We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc. +We have therefore adopted this CoC, which we require all members of our community and attendees of nf-core events to adhere to in all our workspaces at all times. Workspaces include, but are not limited to, Slack, meetings on Zoom, gather.town, YouTube live etc. -Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. +Our CoC will be strictly enforced and the nf-core team reserves the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. -We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. +We ask all members of our community to help maintain supportive and productive workspaces and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. -Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re +Questions, concerns, or ideas on what we can include? Contact members of the Safety Team on Slack or email safety [at] nf-co [dot] re. ## Our Responsibilities -The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. +Members of the Safety Team (the Safety Officers) are responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. -The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. +The Safety Team, in consultation with the nf-core core team, have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this CoC, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. -Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC. +Members of the core team or the Safety Team who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and will be subject to the same actions as others in violation of the CoC. -## When are where does this Code of Conduct apply? +## When and where does this Code of Conduct apply? -Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference: +Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events, such as hackathons, workshops, bytesize, and collaborative workspaces on gather.town. These guidelines include, but are not limited to, the following (listed alphabetically and therefore in no order of preference): - Communicating with an official project email address. - Communicating with community members within the nf-core Slack channel. - Participating in hackathons organised by nf-core (both online and in-person events). -- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence. -- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc. +- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence, and on the nf-core gather.town workspace. +- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, gather.town, Jitsi, YouTube live etc. - Representing nf-core on social media. This includes both official and personal accounts. ## nf-core cares 😊 -nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order): +nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include, but are not limited to, the following (listed in alphabetical order): - Ask for consent before sharing another community member’s personal information (including photographs) on social media. - Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity. -- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) +- Celebrate your accomplishments! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) - Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.) - Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can) - Focus on what is best for the team and the community. (When in doubt, ask) -- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn. +- Accept feedback, yet be unafraid to question, deliberate, and learn. - Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!) -- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**) +- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communication to be kind.**) - Take breaks when you feel like you need them. -- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.) +- Use welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack) ## nf-core frowns on 😕 -The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces. +The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this CoC. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces: - Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom. - “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online. - Spamming or trolling of individuals on social media. -- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention. -- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience. +- Use of sexual or discriminatory imagery, comments, jokes, or unwelcome sexual attention. +- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion, or work experience. ### Online Trolling -The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately. +The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the risk of online trolling. This is unacceptable — reports of such behaviour will be taken very seriously and perpetrators will be excluded from activities immediately. -All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls. +All community members are **required** to ask members of the group they are working with for explicit consent prior to taking screenshots of individuals during video calls. -## Procedures for Reporting CoC violations +## Procedures for reporting CoC violations If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible. -You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s). +You can reach out to members of the Safety Team (Saba Nafees, Cris Tuñí, and Michael Heuer) on Slack. Alternatively, contact a member of the nf-core core team [nf-core core team](https://nf-co.re/about), and they will forward your concerns to the Safety Team. + +Issues directly concerning members of the Core Team or the Safety Team will be dealt with by other members of the core team and the safety manager — possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson and details will be shared in due course. + +All reports will be handled with the utmost discretion and confidentiality. + +You can also report any CoC violations to safety [at] nf-co [dot] re. In your email report, please do your best to include: + +- Your contact information. +- Identifying information (e.g. names, nicknames, pseudonyms) of the participant who has violated the Code of Conduct. +- The behaviour that was in violation and the circumstances surrounding the incident. +- The approximate time of the behaviour (if different than the time the report was made). +- Other people involved in the incident, if applicable. +- If you believe the incident is ongoing. +- If there is a publicly available record (e.g. mailing list record, a screenshot). +- Any additional information. + +After you file a report, one or more members of our Safety Team will contact you to follow up on your report. + +## Who will read and handle reports + +All reports will be read and handled by the members of the Safety Team at nf-core. + +If members of the Safety Team are deemed to have a conflict of interest with a report, they will be required to recuse themselves as per our Code of Conduct and will not have access to any follow-ups. + +To keep this first report confidential from any of the Safety Team members, please submit your first report by direct messaging on Slack/direct email to any of the nf-core members you are comfortable disclosing the information to, and be explicit about which member(s) you do not consent to sharing the information with. + +## Reviewing reports + +After receiving the report, members of the Safety Team will review the incident report to determine whether immediate action is required, for example, whether there is immediate threat to participants’ safety. + +The Safety Team, in consultation with members of the nf-core core team, will assess the information to determine whether the report constitutes a Code of Conduct violation, for them to decide on a course of action. + +In the case of insufficient information, one or more members of the Safety Team may contact the reporter, the reportee, or any other attendees to obtain more information. -Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course. +Once additional information is gathered, the Safety Team will collectively review and decide on the best course of action to take, if any. The Safety Team reserves the right to not act on a report. -All reports will be handled with utmost discretion and confidentially. +## Confidentiality + +All reports, and any additional information included, are only shared with the team of safety officers (and possibly members of the core team, in case the safety officer is in violation of the CoC). We will respect confidentiality requests for the purpose of protecting victims of abuse. + +We will not name harassment victims, beyond discussions between the safety officer and members of the nf-core team, without the explicit consent of the individuals involved. + +## Enforcement + +Actions taken by the nf-core’s Safety Team may include, but are not limited to: + +- Asking anyone to stop a behaviour. +- Asking anyone to leave the event and online spaces either temporarily, for the remainder of the event, or permanently. +- Removing access to the gather.town and Slack, either temporarily or permanently. +- Communicating to all participants to reinforce our expectations for conduct and remind what is unacceptable behaviour; this may be public for practical reasons. +- Communicating to all participants that an incident has taken place and how we will act or have acted — this may be for the purpose of letting event participants know we are aware of and dealing with the incident. +- Banning anyone from participating in nf-core-managed spaces, future events, and activities, either temporarily or permanently. +- No action. ## Attribution and Acknowledgements @@ -106,6 +161,22 @@ All reports will be handled with utmost discretion and confidentially. ## Changelog -### v1.0 - March 12th, 2021 +### v1.4 - February 8th, 2022 + +- Included a new member of the Safety Team. Corrected a typographical error in the text. + +### v1.3 - December 10th, 2021 + +- Added a statement that the CoC applies to nf-core gather.town workspaces. Corrected typographical errors in the text. + +### v1.2 - November 12th, 2021 + +- Removed information specific to reporting CoC violations at the Hackathon in October 2021. + +### v1.1 - October 14th, 2021 + +- Updated with names of new Safety Officers and specific information for the hackathon in October 2021. + +### v1.0 - March 15th, 2021 - Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC. diff --git a/README.md b/README.md index 26c53833..b821a3fb 100644 --- a/README.md +++ b/README.md @@ -1,6 +1,7 @@ # ![nf-core/pangenome](docs/images/nf-core-pangenome_logo_light.png#gh-light-mode-only) ![nf-core/pangenome](docs/images/nf-core-pangenome_logo_dark.png#gh-dark-mode-only) -[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/pangenome/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![GitHub Actions CI Status](https://github.com/nf-core/pangenome/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/pangenome/actions?query=workflow%3A%22nf-core+CI%22) +[![GitHub Actions Linting Status](https://github.com/nf-core/pangenome/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/pangenome/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/pangenome/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) @@ -29,10 +30,11 @@ ## Usage -> **Note** -> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how -> to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) -> with `-profile test` before running the workflow on actual data. +:::note +If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how +to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) +with `-profile test` before running the workflow on actual data. +::: - + +If you use nf-core/pangenome for your analysis, please cite it using the following doi: [10.5281/zenodo.8202636](https://doi.org/10.5281/zenodo.8202636) An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. From 9d4675d08fadcc86dfe5c8709e36deef5611a3bc Mon Sep 17 00:00:00 2001 From: FriederikeHanssen Date: Thu, 12 Oct 2023 12:58:29 +0000 Subject: [PATCH 25/83] more missing citations --- lib/WorkflowMain.groovy | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index d125f409..c5f7930d 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -11,9 +11,8 @@ class WorkflowMain { // public static String citation(workflow) { return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + + "* The pipeline\n" + + " https://doi.org/10.5281/zenodo.8202636\n\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + From 8b3b1d42341a18847a7644ff8030c0b1234b1dff Mon Sep 17 00:00:00 2001 From: Peter Heringer Date: Tue, 17 Oct 2023 11:05:51 +0200 Subject: [PATCH 26/83] Add metric endings for larger numbers See Issue #120 --- conf/modules.config | 61 +++++++++++++++++++++++++++++++++++++------- nextflow_schema.json | 28 ++++++++------------ 2 files changed, 63 insertions(+), 26 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index f2846ae3..abf84190 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -47,6 +47,49 @@ def generate_smoothxg_poa_params_cmd() { def smoothxg_poa_params_cmd = generate_smoothxg_poa_params_cmd() +def parse_int(String s) { + def p = /\d+[KkMmGgTt]?$/ + def out = 890 + if (s ==~ p) { + if (s.isInteger()) { + out = s.toInteger() + } else { + def intti = s.substring(0, s.length() - 1).toInteger() + def suffixi = s.substring(s.length() - 1, s.length()) + //Evaluating the expression value + switch(suffixi) { + //There is case statement defined for 4 cases + // Each case statement section has a break condition to exit the loop + + case "k": + case "K": + out = intti * 1000 + break; + case "m": + case "M": + out = intti * 1000000 + break; + case "g": + case "G": + out = intti * 1000000000 + break; + case "t": + case "T": + out = intti * 1000000000000 + break; + default: + //println("The value is unknown"); + // we will never hit this case + break; + } + } + } else { + // errorrrrr + return 0 + } + return out +} + process { publishDir = [ @@ -67,12 +110,12 @@ process { ext.args = { [ "-n ${params.n_haplotypes - 1}", - "-s ${params.wfmash_segment_length}", + "-s ${parse_int(params.wfmash_segment_length)}", "-p ${params.wfmash_map_pct_id}", params.wfmash_merge_segments ? "-M" : "", params.wfmash_exclude_delim ? "-Y ${params.wfmash_exclude_delim}" : "-X", params.wfmash_no_splits ? "-N" : "", - params.wfmash_block_length ? "-l ${params.wfmash_block_length}" : "-l ${params.wfmash_segment_length * 5}", + params.wfmash_block_length ? "-l ${parse_int(params.wfmash_block_length)}" : "-l ${parse_int(params.wfmash_segment_length) * 5}", "-k ${params.wfmash_mash_kmer}", "-H ${params.wfmash_mash_kmer_thres}", "${wfmash_sparse_map_cmd}", @@ -90,12 +133,12 @@ process { ext.args = { [ "-n ${params.n_haplotypes - 1}", - "-s ${params.wfmash_segment_length}", + "-s ${parse_int(params.wfmash_segment_length)}", "-p ${params.wfmash_map_pct_id}", params.wfmash_merge_segments ? "-M" : "", params.wfmash_exclude_delim ? "-Y ${params.wfmash_exclude_delim}" : "-X", params.wfmash_no_splits ? "-N" : "", - params.wfmash_block_length ? "-l ${params.wfmash_block_length}" : "-l ${params.wfmash_segment_length * 5}", + params.wfmash_block_length ? "-l ${parse_int(params.wfmash_block_length)}" : "-l ${parse_int(params.wfmash_segment_length) * 5}", "-k ${params.wfmash_mash_kmer}", "-H ${params.wfmash_mash_kmer_thres}", "${wfmash_sparse_map_cmd}", @@ -114,12 +157,12 @@ process { ext.args = { [ "-n ${params.n_haplotypes - 1}", - "-s ${params.wfmash_segment_length}", + "-s ${parse_int(params.wfmash_segment_length)}", "-p ${params.wfmash_map_pct_id}", params.wfmash_merge_segments ? "-M" : "", params.wfmash_exclude_delim ? "-Y ${params.wfmash_exclude_delim}" : "-X", params.wfmash_no_splits ? "-N" : "", - params.wfmash_block_length ? "-l ${params.wfmash_block_length}" : "-l ${params.wfmash_segment_length * 5}", + params.wfmash_block_length ? "-l ${parse_int(params.wfmash_block_length)}" : "-l ${parse_int(params.wfmash_segment_length) * 5}", "-k ${params.wfmash_mash_kmer}", "-H ${params.wfmash_mash_kmer_thres}", "${wfmash_sparse_map_cmd}", @@ -147,12 +190,12 @@ process { ext.args = { [ "-n ${params.n_haplotypes - 1}", - "-s ${params.wfmash_segment_length}", + "-s ${parse_int(params.wfmash_segment_length)}", "-p ${params.wfmash_map_pct_id}", params.wfmash_merge_segments ? "-M" : "", params.wfmash_exclude_delim ? "-Y ${params.wfmash_exclude_delim}" : "-X", params.wfmash_no_splits ? "-N" : "", - params.wfmash_block_length ? "-l ${params.wfmash_block_length}" : "-l ${params.wfmash_segment_length * 5}", + params.wfmash_block_length ? "-l ${parse_int(params.wfmash_block_length)}" : "-l ${parse_int(params.wfmash_segment_length) * 5}", "-k ${params.wfmash_mash_kmer}", "-H ${params.wfmash_mash_kmer_thres}", "${wfmash_sparse_map_cmd}", @@ -173,7 +216,7 @@ process { [ "-k ${params.seqwish_min_match_length}", "-f ${params.seqwish_sparse_factor}", - "-B ${params.seqwish_transclose_batch}", + "-B ${parse_int(params.seqwish_transclose_batch)}", "-P", params.seqwish_temp_dir ? "--temp-dir ${params.seqwish_temp_dir}" : "" ].join(" ").trim() diff --git a/nextflow_schema.json b/nextflow_schema.json index 44f6c3fc..b3df45f2 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -59,16 +59,17 @@ "help_text": "Use `mash dist` or `mash triangle` to explore the typical level of divergence between the sequences in your input (see https://pggb.readthedocs.io/en/latest/rst/tutorials/divergence_estimation.html#divergence-estimation for more information). Convert this to an approximate percent identity and provide it as --wfmash_map_pct_id . A list of examples can be found at https://github.com/pangenome/pggb#example-builds-for-diverse-species." }, "wfmash_segment_length": { - "type": "integer", - "default": 5000, + "type": "string", + "default": "5K", "description": "Segment length for mapping.", - "help_text": "Crucially, --wfmash_segment_length provides a kind of minimum alignment length filter. The `mashmap3` step in `wfmash` will only consider segments of this size. For small pangenome graphs, or where there are few repeats, --wfmash_segment_length can be set low (for example 500 when building a MHC pangenome graph). However, for larger contexts, with repeats, it can be very important to set this high (for instance 50k in the case of human genomes). A long segment length ensures that we represent long collinear regions of the input sequences in the structure of the graph. In general, this should at least be larger than transposons and other common repeats in your pangenome. A list of examples can be found at https://github.com/pangenome/pggb#example-builds-for-diverse-species." + "help_text": "Crucially, --wfmash_segment_length provides a kind of minimum alignment length filter. The `mashmap3` step in `wfmash` will only consider segments of this size. For small pangenome graphs, or where there are few repeats, --wfmash_segment_length can be set low (for example 500 when building a MHC pangenome graph). However, for larger contexts, with repeats, it can be very important to set this high (for instance 50k in the case of human genomes). A long segment length ensures that we represent long collinear regions of the input sequences in the structure of the graph. In general, this should at least be larger than transposons and other common repeats in your pangenome. A list of examples can be found at https://github.com/pangenome/pggb#example-builds-for-diverse-species.", + "pattern": "^([1-9]\\d*[kKmMgGtT]?|0)$" }, "wfmash_block_length": { "type": "string", - "default": "wfmash_segment_length * 5", "description": "Minimum block length filter for mapping.", - "help_text": "By default, wfmash only keeps mappings with at least 5 times the size of a segment. This can be adjusted with --wfmash_block_length ." + "help_text": "By default, wfmash only keeps mappings with at least 5 times the size of a segment. This can be adjusted with --wfmash_block_length .", + "pattern": "^([1-9]\\d*[kKmMgGtT]?|0)$" }, "wfmash_mash_kmer": { "type": "integer", @@ -82,7 +83,7 @@ }, "wfmash_sparse_map": { "type": "number", - "default": "1.0", + "default": 1, "description": "Keep this fraction of mappings (\"auto\" for giant component heuristic)." }, "wfmash_merge_segments": { @@ -129,10 +130,11 @@ "help_text": "Graph induction with seqwish often works better when we filter very short matches out of the input alignments. In practice, these often occur in regions of low alignment quality, which are typical of areas with large INDELs and structural variations in the wfmash alignments. This underalignment is then resolved in the smoothxg step. Removing short matches can simplify the graph and remove spurious relationships caused by short repeated homologies.\nA setting of --seqwish_min_match_length 47 is optimal for around 5% divergence, and we suggest lowering it for higher divergence and increasing it for lower divergence. Values up to --seqwish_min_match_length 311 work well for human haplotypes. In effect, setting --seqwish_min_match_length to N means that we can tolerate a local pairwise difference rate of no more than 1/N. Thus, INDELs which may be represented by complex series of edit operations will be opened into bubbles in the induced graph, and alignment regions with very low identity will be ignored. Using affine-gapped alignment (such as with minimap2) may reduce the impact of this step by representing large indels more precisely in the input alignments. However, it remains important due to local inconsistency in alignments in low-complexity sequence." }, "seqwish_transclose_batch": { - "type": "integer", - "default": 10000000, + "type": "string", + "default": "10M", "description": "Number of base pairs to use for transitive closure batch.", - "help_text": "If you run out of memory during the seqwish step, you can lower this value. It will take longer, but it will use less memory." + "help_text": "If you run out of memory during the seqwish step, you can lower this value. It will take longer, but it will use less memory.", + "pattern": "^([1-9]\\d*[kKmMgGtT]?|0)$" }, "seqwish_sparse_factor": { "type": "number", @@ -360,14 +362,12 @@ "type": "boolean", "description": "Display help text.", "fa_icon": "fas fa-question-circle", - "default": false, "hidden": true }, "version": { "type": "boolean", "description": "Display version and exit.", "fa_icon": "fas fa-question-circle", - "default": false, "hidden": true }, "publish_dir_mode": { @@ -391,7 +391,6 @@ "type": "boolean", "description": "Send plain-text email instead of HTML.", "fa_icon": "fas fa-remove-format", - "default": false, "hidden": true }, "max_multiqc_email_size": { @@ -406,7 +405,6 @@ "type": "boolean", "description": "Do not use coloured log outputs.", "fa_icon": "fas fa-palette", - "default": false, "hidden": true }, "hook_url": { @@ -445,7 +443,6 @@ "type": "boolean", "fa_icon": "far fa-eye-slash", "description": "Show all params when using `--help`", - "default": false, "hidden": true, "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." }, @@ -453,7 +450,6 @@ "type": "boolean", "fa_icon": "far fa-check-circle", "description": "Validation of parameters fails when an unrecognised parameter is found.", - "default": false, "hidden": true, "help_text": "By default, when an unrecognised parameter is found, it returns a warinig." }, @@ -461,7 +457,6 @@ "type": "boolean", "fa_icon": "far fa-check-circle", "description": "Validation of parameters in lenient more.", - "default": false, "hidden": true, "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." }, @@ -469,7 +464,6 @@ "type": "boolean", "fa_icon": "far fa-check-circle", "description": "Do we want to display hidden parameters?", - "default": false, "hidden": true, "help_text": "Do we want to display hidden parameters?" }, From e197934253b05b48de4972e4ad69fd60203c82c7 Mon Sep 17 00:00:00 2001 From: Peter Heringer Date: Tue, 17 Oct 2023 11:18:18 +0200 Subject: [PATCH 27/83] Add test to ensure metric parameter suffix handling See Issue #120 --- .github/workflows/ci.yml | 22 ++++++++++++++++++++++ 1 file changed, 22 insertions(+) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index efc70c10..d5e47181 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -151,3 +151,25 @@ jobs: - name: Run pipeline with test data and wfmash_only parameters run: | nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results ${{ matrix.parameters }} + + metric_parameter_suffixes: + name: Run pipeline with metric parameter suffixes + # Only run on push if this is the nf-core dev branch (merged PRs) + if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/pangenome') }}" + runs-on: ubuntu-latest + strategy: + matrix: + parameters: + - "--wfmash_sequence_length 8k --wfmash_block_length 38K --seqwish_transclose_batch 9M" + steps: + - name: Check out pipeline code + uses: actions/checkout@v3 + + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + with: + version: "23.04.0" + + - name: Run pipeline with test data and wfmash_only parameters + run: | + nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results ${{ matrix.parameters }} From b4593b67c20819945c97e8b1293303dc60c0a580 Mon Sep 17 00:00:00 2001 From: Peter Heringer Date: Tue, 17 Oct 2023 15:00:18 +0200 Subject: [PATCH 28/83] Fix parsing of default integers from the config --- conf/modules.config | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/conf/modules.config b/conf/modules.config index 3d57ca34..aa848b13 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -47,6 +47,10 @@ def generate_smoothxg_poa_params_cmd() { def smoothxg_poa_params_cmd = generate_smoothxg_poa_params_cmd() +def parse_int(int s) { + return s +} + def parse_int(String s) { def p = /\d+[KkMmGgTt]?$/ def out = 890 From dc1c16274f2bf66fa5b04de5ab0613adac682a00 Mon Sep 17 00:00:00 2001 From: Peter Heringer Date: Tue, 17 Oct 2023 16:28:26 +0200 Subject: [PATCH 29/83] Fix variable namings and regex pattern --- conf/modules.config | 40 ++++++++++++++++------------------------ nextflow_schema.json | 10 +++++----- 2 files changed, 21 insertions(+), 29 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index aa848b13..c0e57131 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -47,51 +47,43 @@ def generate_smoothxg_poa_params_cmd() { def smoothxg_poa_params_cmd = generate_smoothxg_poa_params_cmd() -def parse_int(int s) { - return s +def parse_int(int plain_integer) { + return plain_integer } -def parse_int(String s) { - def p = /\d+[KkMmGgTt]?$/ - def out = 890 - if (s ==~ p) { - if (s.isInteger()) { - out = s.toInteger() +def parse_int(String integer_with_suffix) { + def pattern = /^([1-9]\d*[kKmMgGtT]?|0)$/ + def parsed_integer + if (integer_with_suffix ==~ pattern) { + if (integer_with_suffix.isInteger()) { + parsed_integer = integer_with_suffix.toInteger() } else { - def intti = s.substring(0, s.length() - 1).toInteger() - def suffixi = s.substring(s.length() - 1, s.length()) - //Evaluating the expression value - switch(suffixi) { - //There is case statement defined for 4 cases - // Each case statement section has a break condition to exit the loop + def integer_part = integer_with_suffix.substring(0, integer_with_suffix.length() - 1).toInteger() + def suffix_part = integer_with_suffix.substring(integer_with_suffix.length() - 1, integer_with_suffix.length()) + switch(suffix_part) { case "k": case "K": - out = intti * 1000 + parsed_integer = integer_part * 1000 break; case "m": case "M": - out = intti * 1000000 + parsed_integer = integer_part * 1000000 break; case "g": case "G": - out = intti * 1000000000 + parsed_integer = integer_part * 1000000000 break; case "t": case "T": - out = intti * 1000000000000 + parsed_integer = integer_part * 1000000000000 break; default: - //println("The value is unknown"); - // we will never hit this case break; } } - } else { - // errorrrrr - return 0 } - return out + return parsed_integer } process { diff --git a/nextflow_schema.json b/nextflow_schema.json index 8b9b6395..c4fa3c4f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -54,13 +54,13 @@ "properties": { "wfmash_map_pct_id": { "type": "number", - "default": 90.0, + "default": 90, "description": "Percent identity in the wfmash mashmap step.", "help_text": "Use `mash dist` or `mash triangle` to explore the typical level of divergence between the sequences in your input (see https://pggb.readthedocs.io/en/latest/rst/tutorials/divergence_estimation.html#divergence-estimation for more information). Convert this to an approximate percent identity and provide it as --wfmash_map_pct_id . A list of examples can be found at https://github.com/pangenome/pggb#example-builds-for-diverse-species." }, "wfmash_segment_length": { "type": "string", - "default": "5K", + "default": "5000", "description": "Segment length for mapping.", "help_text": "Crucially, --wfmash_segment_length provides a kind of minimum alignment length filter. The `mashmap3` step in `wfmash` will only consider segments of this size. For small pangenome graphs, or where there are few repeats, --wfmash_segment_length can be set low (for example 500 when building a MHC pangenome graph). However, for larger contexts, with repeats, it can be very important to set this high (for instance 50k in the case of human genomes). A long segment length ensures that we represent long collinear regions of the input sequences in the structure of the graph. In general, this should at least be larger than transposons and other common repeats in your pangenome. A list of examples can be found at https://github.com/pangenome/pggb#example-builds-for-diverse-species.", "pattern": "^([1-9]\\d*[kKmMgGtT]?|0)$" @@ -82,7 +82,7 @@ "description": "Ignore the top % most-frequent kmers." }, "wfmash_sparse_map": { - "default": 1.0, + "default": "1.0", "description": "Keep this fraction of mappings (\"auto\" for giant component heuristic).", "type": "string", "pattern": "(auto|[01]\\.\\d+)" @@ -132,14 +132,14 @@ }, "seqwish_transclose_batch": { "type": "string", - "default": "10M", + "default": "10000000", "description": "Number of base pairs to use for transitive closure batch.", "help_text": "If you run out of memory during the seqwish step, you can lower this value. It will take longer, but it will use less memory.", "pattern": "^([1-9]\\d*[kKmMgGtT]?|0)$" }, "seqwish_sparse_factor": { "type": "number", - "default": 0.0, + "default": 0, "description": "Keep this randomly selected fraction of input matches." }, "seqwish_temp_dir": { From 49dce5b5ab5481eaec48cc3055b034c2aee42a4c Mon Sep 17 00:00:00 2001 From: Peter Heringer Date: Tue, 17 Oct 2023 16:40:54 +0200 Subject: [PATCH 30/83] Remove unnecessary if --- conf/modules.config | 53 +++++++++++++++++++++----------------------- nextflow_schema.json | 2 +- 2 files changed, 26 insertions(+), 29 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index c0e57131..99aa9b10 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -52,35 +52,32 @@ def parse_int(int plain_integer) { } def parse_int(String integer_with_suffix) { - def pattern = /^([1-9]\d*[kKmMgGtT]?|0)$/ def parsed_integer - if (integer_with_suffix ==~ pattern) { - if (integer_with_suffix.isInteger()) { - parsed_integer = integer_with_suffix.toInteger() - } else { - def integer_part = integer_with_suffix.substring(0, integer_with_suffix.length() - 1).toInteger() - def suffix_part = integer_with_suffix.substring(integer_with_suffix.length() - 1, integer_with_suffix.length()) - - switch(suffix_part) { - case "k": - case "K": - parsed_integer = integer_part * 1000 - break; - case "m": - case "M": - parsed_integer = integer_part * 1000000 - break; - case "g": - case "G": - parsed_integer = integer_part * 1000000000 - break; - case "t": - case "T": - parsed_integer = integer_part * 1000000000000 - break; - default: - break; - } + if (integer_with_suffix.isInteger()) { + parsed_integer = integer_with_suffix.toInteger() + } else { + def integer_part = integer_with_suffix.substring(0, integer_with_suffix.length() - 1).toInteger() + def suffix_part = integer_with_suffix.substring(integer_with_suffix.length() - 1, integer_with_suffix.length()) + + switch(suffix_part) { + case "k": + case "K": + parsed_integer = integer_part * 1000 + break; + case "m": + case "M": + parsed_integer = integer_part * 1000000 + break; + case "g": + case "G": + parsed_integer = integer_part * 1000000000 + break; + case "t": + case "T": + parsed_integer = integer_part * 1000000000000 + break; + default: + break; } } return parsed_integer diff --git a/nextflow_schema.json b/nextflow_schema.json index c4fa3c4f..95e9cd2b 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -139,7 +139,7 @@ }, "seqwish_sparse_factor": { "type": "number", - "default": 0, + "default": 0.0, "description": "Keep this randomly selected fraction of input matches." }, "seqwish_temp_dir": { From e441ca5aa58d3f091275f952c0de3b6c5a2fed5a Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 31 Oct 2023 09:44:08 +0100 Subject: [PATCH 31/83] make the use of chunks explicit --- nextflow.config | 2 +- subworkflows/local/pggb.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow.config b/nextflow.config index 92cca884..dcbbd8c1 100644 --- a/nextflow.config +++ b/nextflow.config @@ -23,7 +23,7 @@ params { wfmash_merge_segments = false wfmash_no_splits = false wfmash_exclude_delim = null - wfmash_chunks = 1 + wfmash_chunks = null wfmash_only = false wfmash_temp_dir = null diff --git a/subworkflows/local/pggb.nf b/subworkflows/local/pggb.nf index 4ba4b932..bb4f35d7 100644 --- a/subworkflows/local/pggb.nf +++ b/subworkflows/local/pggb.nf @@ -62,7 +62,7 @@ workflow PGGB { SEQWISH(ch_combined) ch_versions = ch_versions.mix(SEQWISH.out.versions) } else { - if (params.wfmash_chunks == 1) { + if (params.wfmash_chunks == null) { ch_wfmash_map_align = fasta.map{meta, fasta -> [ meta, fasta, [] ]} ch_wfmash_map_align = ch_wfmash_map_align.join(gzi).join(fai) WFMASH_MAP_ALIGN(ch_wfmash_map_align, From baf2d769d4d57be97bd16bb321f7a60cbce977a7 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 31 Oct 2023 09:55:05 +0100 Subject: [PATCH 32/83] bug fix --- subworkflows/local/pggb.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/pggb.nf b/subworkflows/local/pggb.nf index bb4f35d7..1be30566 100644 --- a/subworkflows/local/pggb.nf +++ b/subworkflows/local/pggb.nf @@ -33,7 +33,7 @@ workflow PGGB { def query_self = true if (params.wfmash_only) { - if (params.wfmash_chunks == 1) { + if (params.wfmash_chunks == null) { ch_wfmash_map_align = fasta.map{meta, fasta -> [ meta, fasta, []]} ch_wfmash_map_align = ch_wfmash_map_align.join(gzi).join(fai) WFMASH_MAP_ALIGN(ch_wfmash_map_align, From 03af9b1586841fc733f24a757d14643aa13944e8 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 19 Dec 2023 19:05:40 +0000 Subject: [PATCH 33/83] Template update for nf-core/tools version 2.11 --- .github/CONTRIBUTING.md | 3 + .github/PULL_REQUEST_TEMPLATE.md | 1 + .github/workflows/ci.yml | 2 +- .github/workflows/fix-linting.yml | 4 +- .github/workflows/linting.yml | 12 +- .gitpod.yml | 4 +- README.md | 17 +-- assets/multiqc_config.yml | 2 +- assets/slackreport.json | 2 +- conf/modules.config | 2 +- docs/usage.md | 4 +- lib/NfcoreTemplate.groovy | 32 ++--- modules.json | 6 +- .../dumpsoftwareversions/environment.yml | 7 ++ .../custom/dumpsoftwareversions/main.nf | 6 +- .../custom/dumpsoftwareversions/meta.yml | 7 +- .../dumpsoftwareversions/tests/main.nf.test | 38 ++++++ .../tests/main.nf.test.snap | 27 +++++ .../dumpsoftwareversions/tests/tags.yml | 2 + modules/nf-core/fastqc/environment.yml | 7 ++ modules/nf-core/fastqc/main.nf | 10 +- modules/nf-core/fastqc/meta.yml | 5 + modules/nf-core/fastqc/tests/main.nf.test | 109 ++++++++++++++++++ .../nf-core/fastqc/tests/main.nf.test.snap | 10 ++ modules/nf-core/fastqc/tests/tags.yml | 2 + modules/nf-core/multiqc/environment.yml | 7 ++ modules/nf-core/multiqc/main.nf | 8 +- modules/nf-core/multiqc/meta.yml | 11 +- modules/nf-core/multiqc/tests/main.nf.test | 63 ++++++++++ modules/nf-core/multiqc/tests/tags.yml | 2 + nextflow.config | 10 +- 31 files changed, 358 insertions(+), 64 deletions(-) create mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml create mode 100644 modules/nf-core/fastqc/environment.yml create mode 100644 modules/nf-core/fastqc/tests/main.nf.test create mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap create mode 100644 modules/nf-core/fastqc/tests/tags.yml create mode 100644 modules/nf-core/multiqc/environment.yml create mode 100644 modules/nf-core/multiqc/tests/main.nf.test create mode 100644 modules/nf-core/multiqc/tests/tags.yml diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 2dfb02e4..5f77d235 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -27,6 +27,9 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests +You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to +receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. + When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 441337eb..5658d1a1 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -19,6 +19,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/pang - [ ] If necessary, also make a PR on the nf-core/pangenome _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. - [ ] `CHANGELOG.md` is updated. diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index caff145d..be09b5d8 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,7 +28,7 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index eaf890cd..0e86e35a 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -13,7 +13,7 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 with: token: ${{ secrets.nf_core_bot_auth_token }} @@ -24,7 +24,7 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install Prettier run: npm install -g prettier @prettier/plugin-php diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index b8bdd214..905c58e4 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,9 +14,9 @@ jobs: EditorConfig: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install editorconfig-checker run: npm install -g editorconfig-checker @@ -27,9 +27,9 @@ jobs: Prettier: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install Prettier run: npm install -g prettier @@ -40,7 +40,7 @@ jobs: PythonBlack: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - name: Check code lints with Black uses: psf/black@stable @@ -71,7 +71,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 diff --git a/.gitpod.yml b/.gitpod.yml index 25488dcc..acf72695 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -4,7 +4,9 @@ tasks: command: | pre-commit install --install-hooks nextflow self-update - + - name: unset JAVA_TOOL_OPTIONS + command: | + unset JAVA_TOOL_OPTIONS vscode: extensions: # based on nf-core.nf-core-extensionpack - codezombiech.gitignore # Language support for .gitignore files diff --git a/README.md b/README.md index b821a3fb..9ac11b88 100644 --- a/README.md +++ b/README.md @@ -30,11 +30,8 @@ ## Usage -:::note -If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how -to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) -with `-profile test` before running the workflow on actual data. -::: +> [!NOTE] +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. - + diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 6aff6438..8173f6d7 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,5 +1,5 @@ report_comment: > - This report has been generated by the nf-core/pangenome + This report has been generated by the nf-core/pangenome analysis pipeline. For information about how to interpret these results, please see the documentation. report_section_order: diff --git a/assets/slackreport.json b/assets/slackreport.json index 0255145f..4ddd75b0 100644 --- a/assets/slackreport.json +++ b/assets/slackreport.json @@ -3,7 +3,7 @@ { "fallback": "Plain-text summary of the attachment.", "color": "<% if (success) { %>good<% } else { %>danger<%} %>", - "author_name": "nf-core/pangenome v${version} - ${runName}", + "author_name": "nf-core/pangenome ${version} - ${runName}", "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", "fields": [ diff --git a/conf/modules.config b/conf/modules.config index 39e81386..d91c6aba 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -39,7 +39,7 @@ process { } withName: 'MULTIQC' { - ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' + ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ path: { "${params.outdir}/multiqc" }, mode: params.publish_dir_mode, diff --git a/docs/usage.md b/docs/usage.md index 4bb5b988..ac41ff5e 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -20,7 +20,7 @@ You will need to create a samplesheet with information about the samples you wou The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz @@ -33,7 +33,7 @@ The pipeline will auto-detect whether a sample is single- or paired-end using th A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index 01b8653d..e248e4c3 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -4,6 +4,7 @@ import org.yaml.snakeyaml.Yaml import groovy.json.JsonOutput +import nextflow.extension.FilesEx class NfcoreTemplate { @@ -141,12 +142,14 @@ class NfcoreTemplate { try { if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } [ 'sendmail', '-t' ].execute() << sendmail_html log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" } catch (all) { // Catch failures and try with plaintext def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { mail_cmd += [ '-A', mqc_report ] } mail_cmd.execute() << email_html @@ -155,14 +158,16 @@ class NfcoreTemplate { } // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() } // @@ -227,15 +232,14 @@ class NfcoreTemplate { // Dump pipeline parameters in a json file // public static void dump_parameters(workflow, params) { - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def output_pf = new File(output_d, "params_${timestamp}.json") + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") def jsonStr = JsonOutput.toJson(params) - output_pf.text = JsonOutput.prettyPrint(jsonStr) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() } // diff --git a/modules.json b/modules.json index 4796a706..02bdd9da 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "bd8092b67b5103bdd52e300f75889442275c3117", + "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "4ab13872435962dadc239979554d13709e20bf29", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml new file mode 100644 index 00000000..f0c63f69 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -0,0 +1,7 @@ +name: custom_dumpsoftwareversions +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.17 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index ebc87273..7685b33c 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : + 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml index c32657de..5f15a5fd 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -1,4 +1,4 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: custom_dumpsoftwareversions description: Custom module used to dump software versions within the nf-core pipeline template keywords: @@ -16,7 +16,6 @@ input: type: file description: YML file containing software versions pattern: "*.yml" - output: - yml: type: file @@ -30,7 +29,9 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@drpatelh" - "@grst" +maintainers: + - "@drpatelh" + - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test new file mode 100644 index 00000000..eec1db10 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -0,0 +1,38 @@ +nextflow_process { + + name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" + script "../main.nf" + process "CUSTOM_DUMPSOFTWAREVERSIONS" + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "dumpsoftwareversions" + tag "custom/dumpsoftwareversions" + + test("Should run without failures") { + when { + process { + """ + def tool1_version = ''' + TOOL1: + tool1: 0.11.9 + '''.stripIndent() + + def tool2_version = ''' + TOOL2: + tool2: 1.9 + '''.stripIndent() + + input[0] = Channel.of(tool1_version, tool2_version).collectFile() + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap new file mode 100644 index 00000000..4274ed57 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -0,0 +1,27 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ], + "1": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "2": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "mqc_yml": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "versions": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "yml": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ] + } + ], + "timestamp": "2023-11-03T14:43:22.157011" + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml new file mode 100644 index 00000000..405aa24a --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml @@ -0,0 +1,2 @@ +custom/dumpsoftwareversions: + - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml new file mode 100644 index 00000000..1787b38a --- /dev/null +++ b/modules/nf-core/fastqc/environment.yml @@ -0,0 +1,7 @@ +name: fastqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 249f9064..9e19a74c 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -2,10 +2,10 @@ process FASTQC { tag "$meta.id" label 'process_medium' - conda "bioconda::fastqc=0.11.9" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' : - 'biocontainers/fastqc:0.11.9--0' }" + 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : + 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" input: tuple val(meta), path(reads) @@ -37,7 +37,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ @@ -49,7 +49,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ } diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml index 4da5bb5a..ee5507e0 100644 --- a/modules/nf-core/fastqc/meta.yml +++ b/modules/nf-core/fastqc/meta.yml @@ -50,3 +50,8 @@ authors: - "@grst" - "@ewels" - "@FelixKrueger" +maintainers: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test new file mode 100644 index 00000000..b9e8f926 --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -0,0 +1,109 @@ +nextflow_process { + + name "Test Process FASTQC" + script "../main.nf" + process "FASTQC" + tag "modules" + tag "modules_nfcore" + tag "fastqc" + + test("Single-Read") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id: 'test', single_end:true ], + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. + // looks like this:
Mon 2 Oct 2023
test.gz
+ // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 + { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, + { assert path(process.out.html.get(0).get(1)).getText().contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + ) + } + } +// TODO +// // +// // Test with paired-end data +// // +// workflow test_fastqc_paired_end { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with interleaved data +// // +// workflow test_fastqc_interleaved { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with bam data +// // +// workflow test_fastqc_bam { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with multiple samples +// // +// workflow test_fastqc_multiple { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with custom prefix +// // +// workflow test_fastqc_custom_prefix { +// input = [ +// [ id:'mysample', single_end:true ], // meta map +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } +} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap new file mode 100644 index 00000000..636a32ce --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -0,0 +1,10 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2023-10-09T23:40:54+0000" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml new file mode 100644 index 00000000..7834294b --- /dev/null +++ b/modules/nf-core/fastqc/tests/tags.yml @@ -0,0 +1,2 @@ +fastqc: + - modules/nf-core/fastqc/** diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml new file mode 100644 index 00000000..bc0bdb5b --- /dev/null +++ b/modules/nf-core/multiqc/environment.yml @@ -0,0 +1,7 @@ +name: multiqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.18 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1fc387be..00cc48d2 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -1,10 +1,10 @@ process MULTIQC { label 'process_single' - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : + 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -25,12 +25,14 @@ process MULTIQC { def args = task.ext.args ?: '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' + def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' """ multiqc \\ --force \\ $args \\ $config \\ $extra_config \\ + $logo \\ . cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f93b5ee5..f1aa660e 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,5 +1,5 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json -name: MultiQC +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: - QC @@ -13,7 +13,6 @@ tools: homepage: https://multiqc.info/ documentation: https://multiqc.info/docs/ licence: ["GPL-3.0-or-later"] - input: - multiqc_files: type: file @@ -31,7 +30,6 @@ input: type: file description: Optional logo file for MultiQC pattern: "*.{png}" - output: - report: type: file @@ -54,3 +52,8 @@ authors: - "@bunop" - "@drpatelh" - "@jfy133" +maintainers: + - "@abhi18av" + - "@bunop" + - "@drpatelh" + - "@jfy133" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test new file mode 100644 index 00000000..c2dad217 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -0,0 +1,63 @@ +nextflow_process { + + name "Test Process MULTIQC" + script "../main.nf" + process "MULTIQC" + tag "modules" + tag "modules_nfcore" + tag "multiqc" + + test("MULTIQC: FASTQC") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } + + test("MULTIQC: FASTQC and a config file") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } +} diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml new file mode 100644 index 00000000..bea6c0d3 --- /dev/null +++ b/modules/nf-core/multiqc/tests/tags.yml @@ -0,0 +1,2 @@ +multiqc: + - modules/nf-core/multiqc/** diff --git a/nextflow.config b/nextflow.config index 9a421ca2..cd03f3c4 100644 --- a/nextflow.config +++ b/nextflow.config @@ -14,7 +14,7 @@ params { input = null // References genome = null - igenomes_base = 's3://ngi-igenomes/igenomes' + igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false @@ -82,6 +82,7 @@ profiles { dumpHashes = true process.beforeScript = 'echo $HOSTNAME' cleanup = false + nextflow.enable.configProcessNamesValidation = true } conda { conda.enabled = true @@ -104,13 +105,13 @@ profiles { } docker { docker.enabled = true - docker.userEmulation = true conda.enabled = false singularity.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false + runOptions = '-u $(id -u):$(id -g)' } arm { docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' @@ -181,7 +182,7 @@ singularity.registry = 'quay.io' // Nextflow plugins plugins { - id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet } // Load igenomes.config if required @@ -204,6 +205,9 @@ env { // Capture exit codes from upstream processes when piping process.shell = ['/bin/bash', '-euo', 'pipefail'] +// Disable process selector warnings by default. Use debug profile to enable warnings. +nextflow.enable.configProcessNamesValidation = false + def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { enabled = true From 49207d8c0cd55f72929586d5ce89936969b980b4 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 20 Dec 2023 15:57:18 +0000 Subject: [PATCH 34/83] Template update for nf-core/tools version 2.11.1 --- .../{release-announcments.yml => release-announcements.yml} | 0 nextflow.config | 4 ++-- 2 files changed, 2 insertions(+), 2 deletions(-) rename .github/workflows/{release-announcments.yml => release-announcements.yml} (100%) diff --git a/.github/workflows/release-announcments.yml b/.github/workflows/release-announcements.yml similarity index 100% rename from .github/workflows/release-announcments.yml rename to .github/workflows/release-announcements.yml diff --git a/nextflow.config b/nextflow.config index cd03f3c4..698963e9 100644 --- a/nextflow.config +++ b/nextflow.config @@ -111,10 +111,10 @@ profiles { shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false - runOptions = '-u $(id -u):$(id -g)' + docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { singularity.enabled = true From 3f309cc41190c79399c160575b4fd3191db4f99a Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 29 Jan 2024 15:12:23 +0000 Subject: [PATCH 35/83] Template update for nf-core/tools version 2.12 --- .editorconfig | 8 + .github/workflows/awsfulltest.yml | 2 +- .github/workflows/awstest.yml | 2 +- .github/workflows/branch.yml | 2 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 67 +++++ .github/workflows/fix-linting.yml | 72 +++-- .github/workflows/linting.yml | 61 +--- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 4 +- .gitpod.yml | 1 + .pre-commit-config.yaml | 7 +- README.md | 8 +- assets/email_template.html | 2 +- assets/email_template.txt | 2 +- assets/nf-core-pangenome_logo_light.png | Bin 80051 -> 120169 bytes docs/images/nf-core-pangenome_logo_dark.png | Bin 80171 -> 30331 bytes docs/images/nf-core-pangenome_logo_light.png | Bin 80051 -> 25699 bytes lib/WorkflowMain.groovy | 16 +- lib/nfcore_external_java_deps.jar | Bin 2291171 -> 0 bytes main.nf | 2 +- modules.json | 6 +- .../dumpsoftwareversions/environment.yml | 2 +- .../custom/dumpsoftwareversions/main.nf | 4 +- .../dumpsoftwareversions/tests/main.nf.test | 7 +- .../tests/main.nf.test.snap | 50 ++-- modules/nf-core/fastqc/tests/main.nf.test | 265 ++++++++++++------ .../nf-core/fastqc/tests/main.nf.test.snap | 12 +- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 6 +- modules/nf-core/multiqc/meta.yml | 1 - modules/nf-core/multiqc/tests/main.nf.test | 48 +++- .../nf-core/multiqc/tests/main.nf.test.snap | 21 ++ nextflow.config | 3 +- pyproject.toml | 17 +- workflows/pangenome.nf | 7 + 36 files changed, 490 insertions(+), 221 deletions(-) create mode 100644 .github/workflows/download_pipeline.yml delete mode 100644 lib/nfcore_external_java_deps.jar create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap diff --git a/.editorconfig b/.editorconfig index b6b31907..9b990088 100644 --- a/.editorconfig +++ b/.editorconfig @@ -22,3 +22,11 @@ indent_size = unset [/assets/email*] indent_size = unset + +# ignore Readme +[README.md] +indent_style = unset + +# ignore python +[*.{py}] +indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index fa3cc15a..9695be11 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 13bfcd1e..8ee95d63 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index fd417a18..64f6a2d1 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v1 + uses: mshick/add-pr-comment@v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index 694e90ec..e37cfda5 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v7 + - uses: actions/stale@v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml new file mode 100644 index 00000000..8611458a --- /dev/null +++ b/.github/workflows/download_pipeline.yml @@ -0,0 +1,67 @@ +name: Test successful pipeline download with 'nf-core download' + +# Run the workflow when: +# - dispatched manually +# - when a PR is opened or reopened to master branch +# - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. +on: + workflow_dispatch: + pull_request: + types: + - opened + branches: + - master + pull_request_target: + branches: + - master + +env: + NXF_ANSI_LOG: false + +jobs: + download: + runs-on: ubuntu-latest + steps: + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + + - uses: actions/setup-python@v5 + with: + python-version: "3.11" + architecture: "x64" + - uses: eWaterCycle/setup-singularity@v7 + with: + singularity-version: 3.8.3 + + - name: Install dependencies + run: | + python -m pip install --upgrade pip + pip install git+https://github.com/nf-core/tools.git@dev + + - name: Get the repository name and current branch set as environment variable + run: | + echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} + echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + + - name: Download the pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + run: | + nf-core download ${{ env.REPO_LOWERCASE }} \ + --revision ${{ env.REPO_BRANCH }} \ + --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ + --compress "none" \ + --container-system 'singularity' \ + --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-cache-utilisation 'amend' \ + --download-configuration + + - name: Inspect download + run: tree ./${{ env.REPOTITLE_LOWERCASE }} + + - name: Run the downloaded pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 0e86e35a..10225054 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -4,7 +4,7 @@ on: types: [created] jobs: - deploy: + fix-linting: # Only run if comment is on a PR with the main repo, and if it contains the magic keywords if: > contains(github.event.comment.html_url, '/pull/') && @@ -13,10 +13,17 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} + # indication that the linting is being fixed + - name: React on comment + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: eyes + # Action runs on the issue comment, so we don't get the PR by default # Use the gh cli to check out the PR - name: Checkout Pull Request @@ -24,32 +31,59 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v4 + # Install and run pre-commit + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + with: + python-version: 3.11 - - name: Install Prettier - run: npm install -g prettier @prettier/plugin-php + - name: Install pre-commit + run: pip install pre-commit - # Check that we actually need to fix something - - name: Run 'prettier --check' - id: prettier_status - run: | - if prettier --check ${GITHUB_WORKSPACE}; then - echo "result=pass" >> $GITHUB_OUTPUT - else - echo "result=fail" >> $GITHUB_OUTPUT - fi + - name: Run pre-commit + id: pre-commit + run: pre-commit run --all-files + continue-on-error: true - - name: Run 'prettier --write' - if: steps.prettier_status.outputs.result == 'fail' - run: prettier --write ${GITHUB_WORKSPACE} + # indication that the linting has finished + - name: react if linting finished succesfully + if: steps.pre-commit.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: "+1" - name: Commit & push changes - if: steps.prettier_status.outputs.result == 'fail' + id: commit-and-push + if: steps.pre-commit.outcome == 'failure' run: | git config user.email "core@nf-co.re" git config user.name "nf-core-bot" git config push.default upstream git add . git status - git commit -m "[automated] Fix linting with Prettier" + git commit -m "[automated] Fix code linting" git push + + - name: react if linting errors were fixed + id: react-if-fixed + if: steps.commit-and-push.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: hooray + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: confused + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + issue-number: ${{ github.event.issue.number }} + body: | + @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually. + See [CI log](https://github.com/nf-core/pangenome/actions/runs/${{ github.run_id }}) for more details. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 905c58e4..81cd098e 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,61 +11,22 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - uses: actions/checkout@v4 - - uses: actions/setup-node@v4 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - uses: actions/setup-node@v4 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.11 + uses: actions/setup-python@v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: 3.11 + cache: "pip" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest @@ -76,7 +37,7 @@ jobs: - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.11" architecture: "x64" @@ -99,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 0bbcd30f..147bcd10 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v2 + uses: dawidd6/action-download-artifact@v3 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 6ad33927..21ac3f06 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -24,7 +24,7 @@ jobs: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +56,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.0.2 + - uses: zentered/bluesky-post-action@v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/.gitpod.yml b/.gitpod.yml index acf72695..363d5b1d 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -7,6 +7,7 @@ tasks: - name: unset JAVA_TOOL_OPTIONS command: | unset JAVA_TOOL_OPTIONS + vscode: extensions: # based on nf-core.nf-core-extensionpack - codezombiech.gitignore # Language support for .gitignore files diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 0c31cdb9..af57081f 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -1,5 +1,10 @@ repos: - repo: https://github.com/pre-commit/mirrors-prettier - rev: "v2.7.1" + rev: "v3.1.0" hooks: - id: prettier + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python + rev: "2.7.3" + hooks: + - id: editorconfig-checker + alias: ec diff --git a/README.md b/README.md index 9ac11b88..a51b5b53 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,9 @@ -# ![nf-core/pangenome](docs/images/nf-core-pangenome_logo_light.png#gh-light-mode-only) ![nf-core/pangenome](docs/images/nf-core-pangenome_logo_dark.png#gh-dark-mode-only) - +

+ + + nf-core/pangenome + +

[![GitHub Actions CI Status](https://github.com/nf-core/pangenome/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/pangenome/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/nf-core/pangenome/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/pangenome/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/pangenome/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) diff --git a/assets/email_template.html b/assets/email_template.html index c346ecbd..67e284ec 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -12,7 +12,7 @@ -

nf-core/pangenome v${version}

+

nf-core/pangenome ${version}

Run Name: $runName

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(workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { @@ -60,4 +62,16 @@ class WorkflowMain { } return null } + + // + // Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } } diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar deleted file mode 100644 index 805c8bb5e4fd43a12a5891eea5a68788309629b0..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 2291171 zcma%i1CS<7mu}m(-Ea4_?dhK8v~AnAZQHhO+qP}n-E-&Lf8*Yb{bM(7MpZ;s){{|L znGpxiIY(9k6buar3JMCSNkdW|=)W{*ATS^)VMTsgF=-L{?{Od?P#{?eNT`1hApZ%I z{U0Zz{YCuW$x{5%Vj{u{igZ#U4^op8k`lCZbMO+hRMV3)4e|_&%)3YSbmCGpVp4NX zni_mKLGakt&P12{r^KCD0GPa9sIuy`fu+agt?8O-hY9i{WloGe}y@kSsDEY_Fud9 z-?2ab4J)MQXe4E3Xl-U<>PRMQZ)0m@@90KnV5#Te5Tz`pvZ0Lj4F<9%Flh(`Qb;Tn zMV!iFmH{S^fF*j#MCKRUd`7m>9||*;WE32I_6pg4-Q1C#ke$$aVmP-TtfJ+j+N9;Q z>bxN=bCVQkg$Jzty71(+~A9}ZfANuYIVnTdW!??Sj)Ti-Qpk8XgPDab8pk2QD&dwUl=iFhd1c$C2uMVagr zWc@z9s$#g__{3?VGb#)^fSNp^lZh zU>MI!D?+L)nyth{@@L0+B&_M8iR08L)G6#PZQLFmvz-9DlZkwRWigRIyI4xYr7>tRJ-N6MlBB`nJQ!!8D)mI2lB3PP;dC{<{Td>^ 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b/modules.json index 02bdd9da..1ff04d69 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", + "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "4ab13872435962dadc239979554d13709e20bf29", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml index f0c63f69..9b3272bc 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.17 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 7685b33c..f2187611 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : - 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test index eec1db10..b1e1630b 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -31,7 +31,12 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + process.out.versions, + file(process.out.mqc_yml[0]).readLines()[0..10], + file(process.out.yml[0]).readLines()[0..7] + ).match() + } ) } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap index 4274ed57..5f59a936 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -1,27 +1,33 @@ { "Should run without failures": { "content": [ - { - "0": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ], - "1": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "2": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "mqc_yml": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "versions": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "yml": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ] - } + [ + "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" + ], + [ + "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", + " \\n\\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n \\n \\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n\\n\\n \\n\\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\" + ], + [ + "CUSTOM_DUMPSOFTWAREVERSIONS:", + " python: 3.11.7", + " yaml: 5.4.1", + "TOOL1:", + " tool1: 0.11.9", + "TOOL2:", + " tool2: '1.9'", + "Workflow:" + ] ], - "timestamp": "2023-11-03T14:43:22.157011" + "timestamp": "2024-01-09T23:01:18.710682" } -} +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index b9e8f926..1f21c664 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -3,24 +3,20 @@ nextflow_process { name "Test Process FASTQC" script "../main.nf" process "FASTQC" + tag "modules" tag "modules_nfcore" tag "fastqc" - test("Single-Read") { + test("sarscov2 single-end [fastq]") { when { - params { - outdir = "$outputDir" - } process { """ - input[0] = [ + input[0] = Channel.of([ [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) """ } } @@ -28,82 +24,189 @@ nextflow_process { then { assertAll ( { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. // looks like this:
Mon 2 Oct 2023
test.gz
// https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, - { assert path(process.out.html.get(0).get(1)).getText().contains("") }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 paired-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 interleaved [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } ) } } -// TODO -// // -// // Test with paired-end data -// // -// workflow test_fastqc_paired_end { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with interleaved data -// // -// workflow test_fastqc_interleaved { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with bam data -// // -// workflow test_fastqc_bam { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with multiple samples -// // -// workflow test_fastqc_multiple { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with custom prefix -// // -// workflow test_fastqc_custom_prefix { -// input = [ -// [ id:'mysample', single_end:true ], // meta map -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } + + test("sarscov2 paired-end [bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 multiple [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + { assert path(process.out.html[0][1][2]).text.contains("") }, + { assert path(process.out.html[0][1][3]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 custom_prefix") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastq] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.html.collect { file(it[1]).getName() } + + process.out.zip.collect { file(it[1]).getName() } + + process.out.versions ).match() } + ) + } + } + } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 636a32ce..5d624bb8 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,10 +1,20 @@ { + "sarscov2 single-end [fastq] - stub": { + "content": [ + [ + "test.html", + "test.zip", + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2024-01-17T18:40:57.254299" + }, "versions": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2023-10-09T23:40:54+0000" + "timestamp": "2024-01-17T18:36:50.033627" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index bc0bdb5b..7625b752 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.18 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 00cc48d2..1b9f7c43 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : - 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -43,7 +43,7 @@ process MULTIQC { stub: """ - touch multiqc_data + mkdir multiqc_data touch multiqc_plots touch multiqc_report.html diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f1aa660e..45a9bc35 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,4 +1,3 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index c2dad217..d0438eda 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -7,12 +7,9 @@ nextflow_process { tag "modules_nfcore" tag "multiqc" - test("MULTIQC: FASTQC") { + test("sarscov2 single-end [fastqc]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -26,20 +23,17 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } ) } } - test("MULTIQC: FASTQC and a config file") { + test("sarscov2 single-end [fastqc] [config]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -53,9 +47,35 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastqc] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.report.collect { file(it).getName() } + + process.out.data.collect { file(it).getName() } + + process.out.plots.collect { file(it).getName() } + + process.out.versions ).match() } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap new file mode 100644 index 00000000..d37e7304 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -0,0 +1,21 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:02:49.911994" + }, + "sarscov2 single-end [fastqc] - stub": { + "content": [ + [ + "multiqc_report.html", + "multiqc_data", + "multiqc_plots", + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:03:14.524346" + } +} \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 698963e9..1b18be51 100644 --- a/nextflow.config +++ b/nextflow.config @@ -71,7 +71,7 @@ try { } // Load nf-core/pangenome custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs! +// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! // try { // includeConfig "${params.custom_config_base}/pipeline/pangenome.config" // } catch (Exception e) { @@ -91,6 +91,7 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false + channels = ['conda-forge', 'bioconda', 'defaults'] apptainer.enabled = false } mamba { diff --git a/pyproject.toml b/pyproject.toml index 0d62beb6..7d08e1c8 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,10 +1,13 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.black] +[tool.ruff] line-length = 120 -target_version = ["py37", "py38", "py39", "py310"] +target-version = "py38" +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] +cache-dir = "~/.cache/ruff" -[tool.isort] -profile = "black" -known_first_party = ["nf_core"] -multi_line_output = 3 +[tool.ruff.isort] +known-first-party = ["nf_core"] + +[tool.ruff.per-file-ignores] +"__init__.py" = ["E402", "F401"] diff --git a/workflows/pangenome.nf b/workflows/pangenome.nf index af77f21e..41ceb16e 100644 --- a/workflows/pangenome.nf +++ b/workflows/pangenome.nf @@ -127,6 +127,13 @@ workflow.onComplete { } } +workflow.onError { + if (workflow.errorReport.contains("Process requirement exceeds available memory")) { + println("🛑 Default resources exceed availability 🛑 ") + println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") + } +} + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END From 87abe94ece841b8447997b30a43429609511f708 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 29 Jan 2024 16:34:33 +0100 Subject: [PATCH 36/83] prettier, but not pretty enough --- .devcontainer/devcontainer.json | 8 ++++---- .github/workflows/download_pipeline.yml | 2 +- 2 files changed, 5 insertions(+), 5 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe3..4a9bc5c7 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -18,11 +18,11 @@ "python.linting.flake8Path": "/opt/conda/bin/flake8", "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.linting.pylintPath": "/opt/conda/bin/pylint", }, // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] - } - } + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"], + }, + }, } diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458a..8a330045 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -64,4 +64,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results From 86cdd25422f345751a9a525abc031fc708c24f0c Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 29 Jan 2024 16:40:23 +0100 Subject: [PATCH 37/83] maybe happy --- lib/WorkflowMain.groovy | 12 ++++++++++++ 1 file changed, 12 insertions(+) diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 5ee69d2d..d9afc927 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -49,4 +49,16 @@ class WorkflowMain { error("Please provide an input FASTA to the pipeline e.g. '--input sequences.fa.gz'") } } + + // + // Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } } From fee6eb043d26b840ca759cb1c022d661a581cc15 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 29 Jan 2024 16:41:32 +0100 Subject: [PATCH 38/83] fix linter --- workflows/pangenome.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/workflows/pangenome.nf b/workflows/pangenome.nf index c8e9d759..5ebc16fa 100644 --- a/workflows/pangenome.nf +++ b/workflows/pangenome.nf @@ -115,9 +115,9 @@ workflow PANGENOME { ch_versions = ch_versions.mix(ODGI_VIEW.out.versions) ch_multiqc_in = PGGB.out.qc.map{meta, seqwish, gfaffix, viz, viz_pos, viz_depth, viz_inv, viz_O, viz_uncalled, draw -> [ meta, [ seqwish, gfaffix, viz, viz_pos, viz_depth, viz_inv, viz_O, viz_uncalled, draw ] ]} MULTIQC_COMMUNITY(ch_multiqc_in, - ch_multiqc_config.toList(), - ch_multiqc_custom_config.toList(), - ch_multiqc_logo.toList()) + ch_multiqc_config.toList(), + ch_multiqc_custom_config.toList(), + ch_multiqc_logo.toList()) } else { PGGB ( INPUT_CHECK.out.fasta, From 905e176c8772699aa1f8476edf5f10bf1444da5f Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 29 Jan 2024 16:47:36 +0100 Subject: [PATCH 39/83] DE(A)VD container --- .devcontainer/devcontainer.json | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4a9bc5c7..68466251 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -22,7 +22,7 @@ }, // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"], - }, - }, + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] + } + } } From 0191e1856b4cecdcc595563e84ec50580e3db606 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 29 Jan 2024 16:54:20 +0100 Subject: [PATCH 40/83] fix most linting errors --- conf/modules.config | 2 +- nextflow.config | 8 ++++---- subworkflows/local/pggb.nf | 4 ++-- 3 files changed, 7 insertions(+), 7 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index f66d9ff0..24d01283 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -22,7 +22,7 @@ def generate_wfmash_sparse_map_cmd() { } wfmash_sparse_map_cmd = "-x ${wfmash_sparse_map_frac}" } else { - if (params.wfmash_sparse_map != null) { + if (params.wfmash_sparse_map != null && params.wfmash_sparse_map != 1.0) { wfmash_sparse_map_cmd = "-x ${params.wfmash_sparse_map}" } } diff --git a/nextflow.config b/nextflow.config index 6c0c375f..8c034fd2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -14,16 +14,16 @@ params { n_haplotypes = 0 // Wfmash options - wfmash_map_pct_id = 90 + wfmash_map_pct_id = 90.0 wfmash_segment_length = 5000 wfmash_block_length = null wfmash_mash_kmer = 19 wfmash_mash_kmer_thres = 0.001 - wfmash_sparse_map = null + wfmash_sparse_map = 1.0 wfmash_merge_segments = false wfmash_no_splits = false wfmash_exclude_delim = null - wfmash_chunks = null + wfmash_chunks = 1 wfmash_only = false wfmash_temp_dir = null @@ -31,7 +31,7 @@ params { seqwish_paf = null seqwish_min_match_length = 19 seqwish_transclose_batch = 10000000 - seqwish_sparse_factor = 0 + seqwish_sparse_factor = 0.0 seqwish_temp_dir = null // Smoothxg options diff --git a/subworkflows/local/pggb.nf b/subworkflows/local/pggb.nf index 1be30566..4ba4b932 100644 --- a/subworkflows/local/pggb.nf +++ b/subworkflows/local/pggb.nf @@ -33,7 +33,7 @@ workflow PGGB { def query_self = true if (params.wfmash_only) { - if (params.wfmash_chunks == null) { + if (params.wfmash_chunks == 1) { ch_wfmash_map_align = fasta.map{meta, fasta -> [ meta, fasta, []]} ch_wfmash_map_align = ch_wfmash_map_align.join(gzi).join(fai) WFMASH_MAP_ALIGN(ch_wfmash_map_align, @@ -62,7 +62,7 @@ workflow PGGB { SEQWISH(ch_combined) ch_versions = ch_versions.mix(SEQWISH.out.versions) } else { - if (params.wfmash_chunks == null) { + if (params.wfmash_chunks == 1) { ch_wfmash_map_align = fasta.map{meta, fasta -> [ meta, fasta, [] ]} ch_wfmash_map_align = ch_wfmash_map_align.join(gzi).join(fai) WFMASH_MAP_ALIGN(ch_wfmash_map_align, From 564c039b43ec68668e07f83554cd8212a2ac9042 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 29 Jan 2024 16:57:47 +0100 Subject: [PATCH 41/83] oh be pretty, oh be smart: don't start --- .devcontainer/devcontainer.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 68466251..4ecfbfe3 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -18,7 +18,7 @@ "python.linting.flake8Path": "/opt/conda/bin/flake8", "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint", + "python.linting.pylintPath": "/opt/conda/bin/pylint" }, // Add the IDs of extensions you want installed when the container is created. From a8f4b2bacd5a25f6b7d85c998c99bfce32f30f1a Mon Sep 17 00:00:00 2001 From: subwaystation Date: Thu, 1 Feb 2024 15:12:03 +0100 Subject: [PATCH 42/83] update modules --- modules.json | 30 +-- modules/nf-core/fastqc/main.nf | 55 ----- modules/nf-core/fastqc/meta.yml | 57 ----- modules/nf-core/fastqc/tests/main.nf.test | 212 ------------------ .../nf-core/fastqc/tests/main.nf.test.snap | 20 -- modules/nf-core/fastqc/tests/tags.yml | 2 - modules/nf-core/gfaffix/environment.yml | 7 + modules/nf-core/gfaffix/main.nf | 2 +- modules/nf-core/gfaffix/meta.yml | 3 +- modules/nf-core/odgi/build/environment.yml | 7 + modules/nf-core/odgi/build/main.nf | 6 +- modules/nf-core/odgi/build/meta.yml | 7 +- modules/nf-core/odgi/build/tests/main.nf.test | 34 +++ .../odgi/build/tests/main.nf.test.snap | 33 +++ .../{fastqc => odgi/draw}/environment.yml | 4 +- modules/nf-core/odgi/draw/main.nf | 6 +- modules/nf-core/odgi/draw/meta.yml | 7 +- modules/nf-core/odgi/draw/test/main.nf.test | 35 +++ .../nf-core/odgi/draw/test/main.nf.test.snap | 33 +++ modules/nf-core/odgi/layout/environment.yml | 7 + modules/nf-core/odgi/layout/main.nf | 6 +- modules/nf-core/odgi/layout/meta.yml | 7 +- .../nf-core/odgi/layout/tests/main.nf.test | 35 +++ .../odgi/layout/tests/main.nf.test.snap | 51 +++++ .../nf-core/odgi/layout/tests/nextflow.config | 7 + modules/nf-core/odgi/sort/environment.yml | 7 + modules/nf-core/odgi/sort/main.nf | 6 +- modules/nf-core/odgi/sort/meta.yml | 7 +- modules/nf-core/odgi/sort/tests/main.nf.test | 34 +++ .../nf-core/odgi/sort/tests/main.nf.test.snap | 33 +++ modules/nf-core/odgi/squeeze/environment.yml | 7 + modules/nf-core/odgi/squeeze/main.nf | 6 +- modules/nf-core/odgi/squeeze/meta.yml | 7 +- .../nf-core/odgi/squeeze/tests/main.nf.test | 34 +++ .../odgi/squeeze/tests/main.nf.test.snap | 33 +++ modules/nf-core/odgi/stats/environment.yml | 7 + modules/nf-core/odgi/stats/main.nf | 6 +- modules/nf-core/odgi/stats/meta.yml | 7 +- modules/nf-core/odgi/stats/tests/main.nf.test | 34 +++ .../odgi/stats/tests/main.nf.test.snap | 39 ++++ modules/nf-core/odgi/unchop/environment.yml | 7 + modules/nf-core/odgi/unchop/main.nf | 6 +- modules/nf-core/odgi/unchop/meta.yml | 7 +- .../nf-core/odgi/unchop/tests/main.nf.test | 34 +++ .../odgi/unchop/tests/main.nf.test.snap | 33 +++ modules/nf-core/odgi/view/environment.yml | 7 + modules/nf-core/odgi/view/main.nf | 6 +- modules/nf-core/odgi/view/meta.yml | 7 +- modules/nf-core/odgi/view/tests/main.nf.test | 34 +++ .../nf-core/odgi/view/tests/main.nf.test.snap | 33 +++ modules/nf-core/odgi/viz/environment.yml | 7 + modules/nf-core/odgi/viz/main.nf | 6 +- modules/nf-core/odgi/viz/meta.yml | 7 +- modules/nf-core/odgi/viz/tests/main.nf.test | 34 +++ .../nf-core/odgi/viz/tests/main.nf.test.snap | 33 +++ .../nf-core/samtools/faidx/environment.yml | 8 + modules/nf-core/samtools/faidx/main.nf | 6 +- modules/nf-core/samtools/faidx/meta.yml | 4 + .../nf-core/seqwish/induce/environment.yml | 7 + modules/nf-core/seqwish/induce/main.nf | 2 +- modules/nf-core/seqwish/induce/meta.yml | 3 +- modules/nf-core/smoothxg/environment.yml | 7 + modules/nf-core/smoothxg/main.nf | 6 +- modules/nf-core/smoothxg/meta.yml | 3 +- modules/nf-core/smoothxg/tests/main.nf.test | 56 +++++ .../nf-core/smoothxg/tests/main.nf.test.snap | 39 ++++ .../nf-core/smoothxg/tests/nextflow.config | 11 + modules/nf-core/smoothxg/tests/tags.yml | 2 + modules/nf-core/tabix/bgzip/environment.yml | 7 + modules/nf-core/tabix/bgzip/main.nf | 2 +- modules/nf-core/tabix/bgzip/meta.yml | 5 + modules/nf-core/wfmash/environment.yml | 7 + modules/nf-core/wfmash/main.nf | 6 +- modules/nf-core/wfmash/meta.yml | 7 +- modules/nf-core/wfmash/tests/main.nf.test | 39 ++++ .../nf-core/wfmash/tests/main.nf.test.snap | 33 +++ modules/nf-core/wfmash/tests/nextflow.config | 12 + modules/nf-core/wfmash/tests/tags.yml | 2 + 78 files changed, 1030 insertions(+), 445 deletions(-) delete mode 100644 modules/nf-core/fastqc/main.nf delete mode 100644 modules/nf-core/fastqc/meta.yml delete mode 100644 modules/nf-core/fastqc/tests/main.nf.test delete mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap delete mode 100644 modules/nf-core/fastqc/tests/tags.yml create mode 100644 modules/nf-core/gfaffix/environment.yml create mode 100644 modules/nf-core/odgi/build/environment.yml create mode 100644 modules/nf-core/odgi/build/tests/main.nf.test create mode 100644 modules/nf-core/odgi/build/tests/main.nf.test.snap rename modules/nf-core/{fastqc => odgi/draw}/environment.yml (61%) create mode 100644 modules/nf-core/odgi/draw/test/main.nf.test create mode 100644 modules/nf-core/odgi/draw/test/main.nf.test.snap create mode 100644 modules/nf-core/odgi/layout/environment.yml create mode 100644 modules/nf-core/odgi/layout/tests/main.nf.test create mode 100644 modules/nf-core/odgi/layout/tests/main.nf.test.snap create mode 100644 modules/nf-core/odgi/layout/tests/nextflow.config create mode 100644 modules/nf-core/odgi/sort/environment.yml create mode 100644 modules/nf-core/odgi/sort/tests/main.nf.test create mode 100644 modules/nf-core/odgi/sort/tests/main.nf.test.snap create mode 100644 modules/nf-core/odgi/squeeze/environment.yml create mode 100644 modules/nf-core/odgi/squeeze/tests/main.nf.test create mode 100644 modules/nf-core/odgi/squeeze/tests/main.nf.test.snap create mode 100644 modules/nf-core/odgi/stats/environment.yml create mode 100644 modules/nf-core/odgi/stats/tests/main.nf.test create mode 100644 modules/nf-core/odgi/stats/tests/main.nf.test.snap create mode 100644 modules/nf-core/odgi/unchop/environment.yml create mode 100644 modules/nf-core/odgi/unchop/tests/main.nf.test create mode 100644 modules/nf-core/odgi/unchop/tests/main.nf.test.snap create mode 100644 modules/nf-core/odgi/view/environment.yml create mode 100644 modules/nf-core/odgi/view/tests/main.nf.test create mode 100644 modules/nf-core/odgi/view/tests/main.nf.test.snap create mode 100644 modules/nf-core/odgi/viz/environment.yml create mode 100644 modules/nf-core/odgi/viz/tests/main.nf.test create mode 100644 modules/nf-core/odgi/viz/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/faidx/environment.yml create mode 100644 modules/nf-core/seqwish/induce/environment.yml create mode 100644 modules/nf-core/smoothxg/environment.yml create mode 100644 modules/nf-core/smoothxg/tests/main.nf.test create mode 100644 modules/nf-core/smoothxg/tests/main.nf.test.snap create mode 100644 modules/nf-core/smoothxg/tests/nextflow.config create mode 100644 modules/nf-core/smoothxg/tests/tags.yml create mode 100644 modules/nf-core/tabix/bgzip/environment.yml create mode 100644 modules/nf-core/wfmash/environment.yml create mode 100644 modules/nf-core/wfmash/tests/main.nf.test create mode 100644 modules/nf-core/wfmash/tests/main.nf.test.snap create mode 100644 modules/nf-core/wfmash/tests/nextflow.config create mode 100644 modules/nf-core/wfmash/tests/tags.yml diff --git a/modules.json b/modules.json index 3fed4959..7b0774ba 100644 --- a/modules.json +++ b/modules.json @@ -12,7 +12,7 @@ }, "gfaffix": { "branch": "master", - "git_sha": "f1839b597eab2cdcdf05dd8a9419581d76aca197", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "multiqc": { @@ -22,72 +22,72 @@ }, "odgi/build": { "branch": "master", - "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", + "git_sha": "a474115d309422f44bdd66e04a0926f3ac6aff47", "installed_by": ["modules"] }, "odgi/draw": { "branch": "master", - "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", + "git_sha": "a474115d309422f44bdd66e04a0926f3ac6aff47", "installed_by": ["modules"] }, "odgi/layout": { "branch": "master", - "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", + "git_sha": "a474115d309422f44bdd66e04a0926f3ac6aff47", "installed_by": ["modules"] }, "odgi/sort": { "branch": "master", - "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", + "git_sha": "a474115d309422f44bdd66e04a0926f3ac6aff47", "installed_by": ["modules"] }, "odgi/squeeze": { "branch": "master", - "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", + "git_sha": "a474115d309422f44bdd66e04a0926f3ac6aff47", "installed_by": ["modules"] }, "odgi/stats": { "branch": "master", - "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", + "git_sha": "a474115d309422f44bdd66e04a0926f3ac6aff47", "installed_by": ["modules"] }, "odgi/unchop": { "branch": "master", - "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", + "git_sha": "a474115d309422f44bdd66e04a0926f3ac6aff47", "installed_by": ["modules"] }, "odgi/view": { "branch": "master", - "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", + "git_sha": "a474115d309422f44bdd66e04a0926f3ac6aff47", "installed_by": ["modules"] }, "odgi/viz": { "branch": "master", - "git_sha": "2d8f8fd533fc945358ce11eede8c5e2c86c36e8d", + "git_sha": "a474115d309422f44bdd66e04a0926f3ac6aff47", "installed_by": ["modules"] }, "samtools/faidx": { "branch": "master", - "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe", + "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", "installed_by": ["modules"] }, "seqwish/induce": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "smoothxg": { "branch": "master", - "git_sha": "d7c40cb04c94c408608ceabfdc14a443915a87fe", + "git_sha": "bd863b09cc7ef834dd62d5fd687e941cac2daf64", "installed_by": ["modules"] }, "tabix/bgzip": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "wfmash": { "branch": "master", - "git_sha": "96fadda088c6e79777ba8eef939e36bb03a69c65", + "git_sha": "8cbeaa5063f08952682517597d4e8d6e35bbcd1a", "installed_by": ["modules"] } } diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf deleted file mode 100644 index 9e19a74c..00000000 --- a/modules/nf-core/fastqc/main.nf +++ /dev/null @@ -1,55 +0,0 @@ -process FASTQC { - tag "$meta.id" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : - 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" - - input: - tuple val(meta), path(reads) - - output: - tuple val(meta), path("*.html"), emit: html - tuple val(meta), path("*.zip") , emit: zip - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - // Make list of old name and new name pairs to use for renaming in the bash while loop - def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } - def rename_to = old_new_pairs*.join(' ').join(' ') - def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') - """ - printf "%s %s\\n" $rename_to | while read old_name new_name; do - [ -f "\${new_name}" ] || ln -s \$old_name \$new_name - done - - fastqc \\ - $args \\ - --threads $task.cpus \\ - $renamed_files - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.html - touch ${prefix}.zip - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml deleted file mode 100644 index ee5507e0..00000000 --- a/modules/nf-core/fastqc/meta.yml +++ /dev/null @@ -1,57 +0,0 @@ -name: fastqc -description: Run FastQC on sequenced reads -keywords: - - quality control - - qc - - adapters - - fastq -tools: - - fastqc: - description: | - FastQC gives general quality metrics about your reads. - It provides information about the quality score distribution - across your reads, the per base sequence content (%A/C/G/T). - You get information about adapter contamination and other - overrepresented sequences. - homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ - documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ - licence: ["GPL-2.0-only"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - html: - type: file - description: FastQC report - pattern: "*_{fastqc.html}" - - zip: - type: file - description: FastQC report archive - pattern: "*_{fastqc.zip}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" -maintainers: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test deleted file mode 100644 index 1f21c664..00000000 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ /dev/null @@ -1,212 +0,0 @@ -nextflow_process { - - name "Test Process FASTQC" - script "../main.nf" - process "FASTQC" - - tag "modules" - tag "modules_nfcore" - tag "fastqc" - - test("sarscov2 single-end [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [ id: 'test', single_end:true ], - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. - // looks like this:
Mon 2 Oct 2023
test.gz
- // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("versions") } - ) - } - } - - test("sarscov2 paired-end [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("") }, - { assert path(process.out.html[0][1][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("versions") } - ) - } - } - - test("sarscov2 interleaved [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("versions") } - ) - } - } - - test("sarscov2 paired-end [bam]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("versions") } - ) - } - } - - test("sarscov2 multiple [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, - { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, - { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("") }, - { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert path(process.out.html[0][1][2]).text.contains("") }, - { assert path(process.out.html[0][1][3]).text.contains("") }, - - { assert snapshot(process.out.versions).match("versions") } - ) - } - } - - test("sarscov2 custom_prefix") { - - when { - process { - """ - input[0] = Channel.of([ - [ id:'mysample', single_end:true ], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("versions") } - ) - } - } - - test("sarscov2 single-end [fastq] - stub") { - - options "-stub" - - when { - process { - """ - input[0] = Channel.of([ - [ id: 'test', single_end:true ], - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert snapshot(process.out.html.collect { file(it[1]).getName() } + - process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match() } - ) - } - } - -} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap deleted file mode 100644 index 5d624bb8..00000000 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ /dev/null @@ -1,20 +0,0 @@ -{ - "sarscov2 single-end [fastq] - stub": { - "content": [ - [ - "test.html", - "test.zip", - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "timestamp": "2024-01-17T18:40:57.254299" - }, - "versions": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "timestamp": "2024-01-17T18:36:50.033627" - } -} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml deleted file mode 100644 index 7834294b..00000000 --- a/modules/nf-core/fastqc/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -fastqc: - - modules/nf-core/fastqc/** diff --git a/modules/nf-core/gfaffix/environment.yml b/modules/nf-core/gfaffix/environment.yml new file mode 100644 index 00000000..09e63d24 --- /dev/null +++ b/modules/nf-core/gfaffix/environment.yml @@ -0,0 +1,7 @@ +name: gfaffix +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gfaffix=0.1.5 diff --git a/modules/nf-core/gfaffix/main.nf b/modules/nf-core/gfaffix/main.nf index 64f85bc8..561e02d8 100644 --- a/modules/nf-core/gfaffix/main.nf +++ b/modules/nf-core/gfaffix/main.nf @@ -2,7 +2,7 @@ process GFAFFIX { tag "$meta.id" label 'process_single' - conda "bioconda::gfaffix=0.1.5" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gfaffix:0.1.5--h031d066_0 ' : diff --git a/modules/nf-core/gfaffix/meta.yml b/modules/nf-core/gfaffix/meta.yml index 0c3924a3..384696cf 100644 --- a/modules/nf-core/gfaffix/meta.yml +++ b/modules/nf-core/gfaffix/meta.yml @@ -23,7 +23,6 @@ input: type: file description: Variation graph in GFA format pattern: "*.{gfa}" - output: - meta: type: map @@ -44,3 +43,5 @@ output: pattern: "versions.yml" authors: - "@heuermh" +maintainers: + - "@heuermh" diff --git a/modules/nf-core/odgi/build/environment.yml b/modules/nf-core/odgi/build/environment.yml new file mode 100644 index 00000000..4ae56d26 --- /dev/null +++ b/modules/nf-core/odgi/build/environment.yml @@ -0,0 +1,7 @@ +name: odgi_build +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::odgi=0.8.4 diff --git a/modules/nf-core/odgi/build/main.nf b/modules/nf-core/odgi/build/main.nf index 938c16bb..c1bf3402 100644 --- a/modules/nf-core/odgi/build/main.nf +++ b/modules/nf-core/odgi/build/main.nf @@ -2,10 +2,10 @@ process ODGI_BUILD { tag "$meta.id" label 'process_medium' - conda "bioconda::odgi=0.8.3" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': - 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.4--py310h6cc9453_0': + 'biocontainers/odgi:0.8.4--py310h6cc9453_0' }" input: tuple val(meta), path(graph) diff --git a/modules/nf-core/odgi/build/meta.yml b/modules/nf-core/odgi/build/meta.yml index f37a95e2..8ec55074 100644 --- a/modules/nf-core/odgi/build/meta.yml +++ b/modules/nf-core/odgi/build/meta.yml @@ -12,8 +12,7 @@ tools: documentation: "https://odgi.readthedocs.io/en/latest/" tool_dev_url: "https://github.com/pangenome/odgi" doi: "10.1093/bioinformatics/btac308" - licence: "['MIT']" - + licence: ["MIT"] input: - meta: type: map @@ -24,7 +23,6 @@ input: type: file description: Pangenome graph in GFA v1.0 format pattern: "*.{gfa}" - output: - meta: type: map @@ -39,6 +37,7 @@ output: type: file description: File containing a pangenome graph in ODGI binary format. Usually ends with '.og' pattern: "*.{og}" - authors: - "@subwaystation" +maintainers: + - "@subwaystation" diff --git a/modules/nf-core/odgi/build/tests/main.nf.test b/modules/nf-core/odgi/build/tests/main.nf.test new file mode 100644 index 00000000..5d185c37 --- /dev/null +++ b/modules/nf-core/odgi/build/tests/main.nf.test @@ -0,0 +1,34 @@ +nextflow_process { + + name "Test Process ODGI_BUILD" + script "../main.nf" + process "ODGI_BUILD" + + tag "modules" + tag "modules_nfcore" + tag "odgi" + tag "odgi/build" + + test("homo_sapiens - pangenome - pangenome_smoothxg_gfa") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['pangenome']['pangenome_smoothxg_gfa'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, +// { assert snapshot(process.out).match() } // the odgi file on disk may not be deterministic, but the graph itself is + ) + } + + } + +} diff --git a/modules/nf-core/odgi/build/tests/main.nf.test.snap b/modules/nf-core/odgi/build/tests/main.nf.test.snap new file mode 100644 index 00000000..f2254ad0 --- /dev/null +++ b/modules/nf-core/odgi/build/tests/main.nf.test.snap @@ -0,0 +1,33 @@ +{ + "homo_sapiens - pangenome - pangenome_smoothxg_gfa": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.og:md5,8660c0f724a54d7346575ac38ff0b329" + ] + ], + "1": [ + "versions.yml:md5,57e730c18f2ab7d51bd8ca113e39d553" + ], + "og": [ + [ + { + "id": "test", + "single_end": false + }, + "test.og:md5,8660c0f724a54d7346575ac38ff0b329" + ] + ], + "versions": [ + "versions.yml:md5,57e730c18f2ab7d51bd8ca113e39d553" + ] + } + ], + "timestamp": "2024-01-30T11:07:44.462811986" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/odgi/draw/environment.yml similarity index 61% rename from modules/nf-core/fastqc/environment.yml rename to modules/nf-core/odgi/draw/environment.yml index 1787b38a..2424dff6 100644 --- a/modules/nf-core/fastqc/environment.yml +++ b/modules/nf-core/odgi/draw/environment.yml @@ -1,7 +1,7 @@ -name: fastqc +name: odgi_draw channels: - conda-forge - bioconda - defaults dependencies: - - bioconda::fastqc=0.12.1 + - bioconda::odgi=0.8.4 diff --git a/modules/nf-core/odgi/draw/main.nf b/modules/nf-core/odgi/draw/main.nf index 426c258e..fa1de741 100644 --- a/modules/nf-core/odgi/draw/main.nf +++ b/modules/nf-core/odgi/draw/main.nf @@ -2,10 +2,10 @@ process ODGI_DRAW { tag "$meta.id" label 'process_single' - conda "bioconda::odgi=0.8.3" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': - 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.4--py310h6cc9453_0': + 'biocontainers/odgi:0.8.4--py310h6cc9453_0' }" input: tuple val(meta), path(graph), path(lay) diff --git a/modules/nf-core/odgi/draw/meta.yml b/modules/nf-core/odgi/draw/meta.yml index 5c32b36b..807463ac 100644 --- a/modules/nf-core/odgi/draw/meta.yml +++ b/modules/nf-core/odgi/draw/meta.yml @@ -12,8 +12,7 @@ tools: documentation: "https://odgi.readthedocs.io/en/latest/" tool_dev_url: "https://github.com/pangenome/odgi" doi: "10.1093/bioinformatics/btac308" - licence: "['MIT']" - + licence: ["MIT"] input: - meta: type: map @@ -28,7 +27,6 @@ input: type: file description: 2D layout from 'odgi layout' in LAY binary format pattern: "*.{lay}" - output: - meta: type: map @@ -43,6 +41,7 @@ output: type: file description: File in PNG format containing a 2D drawing of a pangenome graph pattern: "*.{png}" - authors: - "@subwaystation" +maintainers: + - "@subwaystation" diff --git a/modules/nf-core/odgi/draw/test/main.nf.test b/modules/nf-core/odgi/draw/test/main.nf.test new file mode 100644 index 00000000..0306ccb8 --- /dev/null +++ b/modules/nf-core/odgi/draw/test/main.nf.test @@ -0,0 +1,35 @@ +nextflow_process { + + name "Test Process ODGI_DRAW" + script "../main.nf" + process "ODGI_DRAW" + + tag "modules" + tag "modules_nfcore" + tag "odgi" + tag "odgi/draw" + + test("homo_sapiens - pangenome - odgi - pangenome_og - pangenome_lay") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['pangenome']['odgi']['pangenome_og'], checkIfExists: true), + file(params.test_data['homo_sapiens']['pangenome']['odgi']['pangenome_lay'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/odgi/draw/test/main.nf.test.snap b/modules/nf-core/odgi/draw/test/main.nf.test.snap new file mode 100644 index 00000000..f81580f1 --- /dev/null +++ b/modules/nf-core/odgi/draw/test/main.nf.test.snap @@ -0,0 +1,33 @@ +{ + "homo_sapiens - pangenome - odgi - pangenome_og - pangenome_lay": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.png:md5,2024bc28ff028163094fa1070da028db" + ] + ], + "1": [ + "versions.yml:md5,d1ce3da3324df7e6c4531385ad24dbb3" + ], + "png": [ + [ + { + "id": "test", + "single_end": false + }, + "test.png:md5,2024bc28ff028163094fa1070da028db" + ] + ], + "versions": [ + "versions.yml:md5,d1ce3da3324df7e6c4531385ad24dbb3" + ] + } + ], + "timestamp": "2024-01-30T11:03:39.412763234" + } +} \ No newline at end of file diff --git a/modules/nf-core/odgi/layout/environment.yml b/modules/nf-core/odgi/layout/environment.yml new file mode 100644 index 00000000..82212fc8 --- /dev/null +++ b/modules/nf-core/odgi/layout/environment.yml @@ -0,0 +1,7 @@ +name: odgi_layout +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::odgi=0.8.4 diff --git a/modules/nf-core/odgi/layout/main.nf b/modules/nf-core/odgi/layout/main.nf index 22e7c438..27ae4ae0 100644 --- a/modules/nf-core/odgi/layout/main.nf +++ b/modules/nf-core/odgi/layout/main.nf @@ -2,10 +2,10 @@ process ODGI_LAYOUT { tag "$meta.id" label 'process_medium' - conda "bioconda::odgi=0.8.3" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': - 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.4--py310h6cc9453_0': + 'biocontainers/odgi:0.8.4--py310h6cc9453_0' }" input: tuple val(meta), path(graph) diff --git a/modules/nf-core/odgi/layout/meta.yml b/modules/nf-core/odgi/layout/meta.yml index 5c65794a..5d7694a3 100644 --- a/modules/nf-core/odgi/layout/meta.yml +++ b/modules/nf-core/odgi/layout/meta.yml @@ -12,8 +12,7 @@ tools: documentation: "https://odgi.readthedocs.io/en/latest/" tool_dev_url: "https://github.com/pangenome/odgi" doi: "10.1093/bioinformatics/btac308" - licence: "['MIT']" - + licence: ["MIT"] input: - meta: type: map @@ -24,7 +23,6 @@ input: type: file description: Pangenome graph in GFA v1.0 format or in ODGI binary format pattern: "*.{gfa,og}" - output: - meta: type: map @@ -43,6 +41,7 @@ output: type: file description: File containing a 2D layout of a pangenome graph in TSV format. Optional output specified by the `--tsv FILE` argument. Either this or the binary layout output must be specified. pattern: "*.{tsv}" - authors: - "@subwaystation" +maintainers: + - "@subwaystation" diff --git a/modules/nf-core/odgi/layout/tests/main.nf.test b/modules/nf-core/odgi/layout/tests/main.nf.test new file mode 100644 index 00000000..67f915a3 --- /dev/null +++ b/modules/nf-core/odgi/layout/tests/main.nf.test @@ -0,0 +1,35 @@ +nextflow_process { + + name "Test Process ODGI_LAYOUT" + script "../main.nf" + process "ODGI_LAYOUT" + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "odgi" + tag "odgi/layout" + + test("homo_sapiens - pangenome - pangenome_smoothxg_gfa") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['pangenome']['pangenome_smoothxg_gfa'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, +// { assert snapshot(process.out).match() } // odgi layout is not deterministic + ) + } + + } + +} diff --git a/modules/nf-core/odgi/layout/tests/main.nf.test.snap b/modules/nf-core/odgi/layout/tests/main.nf.test.snap new file mode 100644 index 00000000..df8dcf35 --- /dev/null +++ b/modules/nf-core/odgi/layout/tests/main.nf.test.snap @@ -0,0 +1,51 @@ +{ + "homo_sapiens - pangenome - pangenome_smoothxg_gfa": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.lay:md5,ce4ee2f434717077f5c765a5a82fd978" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.tsv:md5,7bf353889ac6dfcce8b38618d760f144" + ] + ], + "2": [ + "versions.yml:md5,c0207add403de5e7011f368a4cfd3b2b" + ], + "lay": [ + [ + { + "id": "test", + "single_end": false + }, + "test.lay:md5,ce4ee2f434717077f5c765a5a82fd978" + ] + ], + "tsv": [ + [ + { + "id": "test", + "single_end": false + }, + "test.tsv:md5,7bf353889ac6dfcce8b38618d760f144" + ] + ], + "versions": [ + "versions.yml:md5,c0207add403de5e7011f368a4cfd3b2b" + ] + } + ], + "timestamp": "2024-01-30T10:58:20.958731752" + } +} \ No newline at end of file diff --git a/modules/nf-core/odgi/layout/tests/nextflow.config b/modules/nf-core/odgi/layout/tests/nextflow.config new file mode 100644 index 00000000..d1ba5054 --- /dev/null +++ b/modules/nf-core/odgi/layout/tests/nextflow.config @@ -0,0 +1,7 @@ +process { + + withName: ODGI_LAYOUT { + ext.args = { "--out ${meta.id}.lay --tsv ${meta.id}.tsv" } + } + +} diff --git a/modules/nf-core/odgi/sort/environment.yml b/modules/nf-core/odgi/sort/environment.yml new file mode 100644 index 00000000..1c1abb89 --- /dev/null +++ b/modules/nf-core/odgi/sort/environment.yml @@ -0,0 +1,7 @@ +name: odgi_sort +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::odgi=0.8.4 diff --git a/modules/nf-core/odgi/sort/main.nf b/modules/nf-core/odgi/sort/main.nf index d57cc04a..06462495 100644 --- a/modules/nf-core/odgi/sort/main.nf +++ b/modules/nf-core/odgi/sort/main.nf @@ -2,10 +2,10 @@ process ODGI_SORT { tag "$meta.id" label 'process_high' - conda "bioconda::odgi=0.8.3" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': - 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.4--py310h6cc9453_0': + 'biocontainers/odgi:0.8.4--py310h6cc9453_0' }" input: tuple val(meta), path(graph) diff --git a/modules/nf-core/odgi/sort/meta.yml b/modules/nf-core/odgi/sort/meta.yml index c29dd91c..792dc381 100644 --- a/modules/nf-core/odgi/sort/meta.yml +++ b/modules/nf-core/odgi/sort/meta.yml @@ -12,8 +12,7 @@ tools: documentation: "https://odgi.readthedocs.io/en/latest/" tool_dev_url: "https://github.com/pangenome/odgi" doi: "10.1093/bioinformatics/btac308" - licence: "['MIT']" - + licence: ["MIT"] input: - meta: type: map @@ -24,7 +23,6 @@ input: type: file description: Pangenome graph in GFA v1.0 format or in ODGI binary format pattern: "*.{gfa,og}" - output: - meta: type: map @@ -39,6 +37,7 @@ output: type: file description: 1D sorted pangenome graph in ODGI binary format pattern: "*.{og}" - authors: - "@subwaystation" +maintainers: + - "@subwaystation" diff --git a/modules/nf-core/odgi/sort/tests/main.nf.test b/modules/nf-core/odgi/sort/tests/main.nf.test new file mode 100644 index 00000000..8245c9e5 --- /dev/null +++ b/modules/nf-core/odgi/sort/tests/main.nf.test @@ -0,0 +1,34 @@ +nextflow_process { + + name "Test Process ODGI_SORT" + script "../main.nf" + process "ODGI_SORT" + + tag "modules" + tag "modules_nfcore" + tag "odgi" + tag "odgi/sort" + + test("homo_sapiens - pangenome - pangenome_smoothxg_gfa") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['pangenome']['pangenome_smoothxg_gfa'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/odgi/sort/tests/main.nf.test.snap b/modules/nf-core/odgi/sort/tests/main.nf.test.snap new file mode 100644 index 00000000..88bcc958 --- /dev/null +++ b/modules/nf-core/odgi/sort/tests/main.nf.test.snap @@ -0,0 +1,33 @@ +{ + "homo_sapiens - pangenome - pangenome_smoothxg_gfa": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.og:md5,91a02c7320969d66bc862cdea317e92e" + ] + ], + "1": [ + "versions.yml:md5,6defcba99b79fde2042de15eec929bf4" + ], + "sorted_graph": [ + [ + { + "id": "test", + "single_end": false + }, + "test.og:md5,91a02c7320969d66bc862cdea317e92e" + ] + ], + "versions": [ + "versions.yml:md5,6defcba99b79fde2042de15eec929bf4" + ] + } + ], + "timestamp": "2024-01-30T10:52:27.598708358" + } +} \ No newline at end of file diff --git a/modules/nf-core/odgi/squeeze/environment.yml b/modules/nf-core/odgi/squeeze/environment.yml new file mode 100644 index 00000000..298458f0 --- /dev/null +++ b/modules/nf-core/odgi/squeeze/environment.yml @@ -0,0 +1,7 @@ +name: odgi_squeeze +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::odgi=0.8.4 diff --git a/modules/nf-core/odgi/squeeze/main.nf b/modules/nf-core/odgi/squeeze/main.nf index a2e187fe..6872fb65 100644 --- a/modules/nf-core/odgi/squeeze/main.nf +++ b/modules/nf-core/odgi/squeeze/main.nf @@ -2,10 +2,10 @@ process ODGI_SQUEEZE { tag "$meta.id" label 'process_medium' - conda "bioconda::odgi=0.8.3" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': - 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.4--py310h6cc9453_0': + 'biocontainers/odgi:0.8.4--py310h6cc9453_0' }" input: tuple val(meta), path(graphs) diff --git a/modules/nf-core/odgi/squeeze/meta.yml b/modules/nf-core/odgi/squeeze/meta.yml index 4b6181d3..5ffaa618 100644 --- a/modules/nf-core/odgi/squeeze/meta.yml +++ b/modules/nf-core/odgi/squeeze/meta.yml @@ -12,8 +12,7 @@ tools: documentation: "https://odgi.readthedocs.io/en/latest/" tool_dev_url: "https://github.com/pangenome/odgi" doi: "10.1093/bioinformatics/btac308" - licence: "['MIT']" - + licence: ["MIT"] input: - meta: type: map @@ -24,7 +23,6 @@ input: type: file description: Pangenome graph files in ODGI format. pattern: "*.{og}" - output: - meta: type: map @@ -39,6 +37,7 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@subwaystation" +maintainers: + - "@subwaystation" diff --git a/modules/nf-core/odgi/squeeze/tests/main.nf.test b/modules/nf-core/odgi/squeeze/tests/main.nf.test new file mode 100644 index 00000000..05fb5a8d --- /dev/null +++ b/modules/nf-core/odgi/squeeze/tests/main.nf.test @@ -0,0 +1,34 @@ +nextflow_process { + + name "Test Process ODGI_SQUEEZE" + script "../main.nf" + process "ODGI_SQUEEZE" + + tag "modules" + tag "modules_nfcore" + tag "odgi" + tag "odgi/squeeze" + + test("homo_sapiens - pangenome - odgi - pangenome_og") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['pangenome']['odgi']['pangenome_og'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/odgi/squeeze/tests/main.nf.test.snap b/modules/nf-core/odgi/squeeze/tests/main.nf.test.snap new file mode 100644 index 00000000..2601eb12 --- /dev/null +++ b/modules/nf-core/odgi/squeeze/tests/main.nf.test.snap @@ -0,0 +1,33 @@ +{ + "homo_sapiens - pangenome - odgi - pangenome_og": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.og:md5,91a02c7320969d66bc862cdea317e92e" + ] + ], + "1": [ + "versions.yml:md5,ee7ed77636f98e98a3e40a915c22ed64" + ], + "graph": [ + [ + { + "id": "test", + "single_end": false + }, + "test.og:md5,91a02c7320969d66bc862cdea317e92e" + ] + ], + "versions": [ + "versions.yml:md5,ee7ed77636f98e98a3e40a915c22ed64" + ] + } + ], + "timestamp": "2024-01-30T10:48:02.189347916" + } +} \ No newline at end of file diff --git a/modules/nf-core/odgi/stats/environment.yml b/modules/nf-core/odgi/stats/environment.yml new file mode 100644 index 00000000..e404f09f --- /dev/null +++ b/modules/nf-core/odgi/stats/environment.yml @@ -0,0 +1,7 @@ +name: odgi_stats +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::odgi=0.8.4 diff --git a/modules/nf-core/odgi/stats/main.nf b/modules/nf-core/odgi/stats/main.nf index 99409f65..7848cfc3 100644 --- a/modules/nf-core/odgi/stats/main.nf +++ b/modules/nf-core/odgi/stats/main.nf @@ -2,10 +2,10 @@ process ODGI_STATS { tag "$meta.id" label 'process_single' - conda "bioconda::odgi=0.8.3" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': - 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.4--py310h6cc9453_0': + 'biocontainers/odgi:0.8.4--py310h6cc9453_0' }" input: tuple val(meta), path(graph) diff --git a/modules/nf-core/odgi/stats/meta.yml b/modules/nf-core/odgi/stats/meta.yml index b80fb195..e8e26e02 100644 --- a/modules/nf-core/odgi/stats/meta.yml +++ b/modules/nf-core/odgi/stats/meta.yml @@ -12,8 +12,7 @@ tools: documentation: "https://odgi.readthedocs.io/en/latest/" tool_dev_url: "https://github.com/pangenome/odgi" doi: "10.1093/bioinformatics/btac308" - licence: "['MIT']" - + licence: ["MIT"] input: - meta: type: map @@ -24,7 +23,6 @@ input: type: file description: Pangenome graph in binary ODGI or in GFA v1.0 format pattern: "*.{og,gfa}" - output: - meta: type: map @@ -43,6 +41,7 @@ output: type: file description: Optional output file that contains graph statistics in YAML format. pattern: "*.{yaml}" - authors: - "@subwaystation" +maintainers: + - "@subwaystation" diff --git a/modules/nf-core/odgi/stats/tests/main.nf.test b/modules/nf-core/odgi/stats/tests/main.nf.test new file mode 100644 index 00000000..72e08774 --- /dev/null +++ b/modules/nf-core/odgi/stats/tests/main.nf.test @@ -0,0 +1,34 @@ +nextflow_process { + + name "Test Process ODGI_STATS" + script "../main.nf" + process "ODGI_STATS" + + tag "modules" + tag "modules_nfcore" + tag "odgi" + tag "odgi/stats" + + test("homo_sapiens - pangenome - pangenome_smoothxg_gfa") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['pangenome']['pangenome_smoothxg_gfa'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/odgi/stats/tests/main.nf.test.snap b/modules/nf-core/odgi/stats/tests/main.nf.test.snap new file mode 100644 index 00000000..c7221489 --- /dev/null +++ b/modules/nf-core/odgi/stats/tests/main.nf.test.snap @@ -0,0 +1,39 @@ +{ + "homo_sapiens - pangenome - pangenome_smoothxg_gfa": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test..og.stats.tsv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,0f4d685d1c0d3562081073bfbf85d95d" + ], + "tsv": [ + [ + { + "id": "test", + "single_end": false + }, + "test..og.stats.tsv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,0f4d685d1c0d3562081073bfbf85d95d" + ], + "yaml": [ + + ] + } + ], + "timestamp": "2024-01-30T10:45:11.453785835" + } +} \ No newline at end of file diff --git a/modules/nf-core/odgi/unchop/environment.yml b/modules/nf-core/odgi/unchop/environment.yml new file mode 100644 index 00000000..1dadcdc9 --- /dev/null +++ b/modules/nf-core/odgi/unchop/environment.yml @@ -0,0 +1,7 @@ +name: odgi_unchop +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::odgi=0.8.4 diff --git a/modules/nf-core/odgi/unchop/main.nf b/modules/nf-core/odgi/unchop/main.nf index 50cbfde2..033011c4 100644 --- a/modules/nf-core/odgi/unchop/main.nf +++ b/modules/nf-core/odgi/unchop/main.nf @@ -2,10 +2,10 @@ process ODGI_UNCHOP { tag "$meta.id" label 'process_medium' - conda "bioconda::odgi=0.8.3" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': - 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.4--py310h6cc9453_0': + 'biocontainers/odgi:0.8.4--py310h6cc9453_0' }" input: tuple val(meta), path(graph) diff --git a/modules/nf-core/odgi/unchop/meta.yml b/modules/nf-core/odgi/unchop/meta.yml index c704f863..5dc31f06 100644 --- a/modules/nf-core/odgi/unchop/meta.yml +++ b/modules/nf-core/odgi/unchop/meta.yml @@ -12,8 +12,7 @@ tools: documentation: "https://odgi.readthedocs.io/en/latest/" tool_dev_url: "https://github.com/pangenome/odgi" doi: "10.1093/bioinformatics/btac308" - licence: "['MIT']" - + licence: ["MIT"] input: - meta: type: map @@ -24,7 +23,6 @@ input: type: file description: Pangenome graph in GFA v1.0 format or in ODGI binary format pattern: "*.{gfa,og}" - output: - meta: type: map @@ -39,6 +37,7 @@ output: type: file description: Unchopped pangenome graph in ODGI binary format pattern: "*.{og}" - authors: - "@subwaystation" +maintainers: + - "@subwaystation" diff --git a/modules/nf-core/odgi/unchop/tests/main.nf.test b/modules/nf-core/odgi/unchop/tests/main.nf.test new file mode 100644 index 00000000..bc940498 --- /dev/null +++ b/modules/nf-core/odgi/unchop/tests/main.nf.test @@ -0,0 +1,34 @@ +nextflow_process { + + name "Test Process ODGI_UNCHOP" + script "../main.nf" + process "ODGI_UNCHOP" + + tag "modules" + tag "modules_nfcore" + tag "odgi" + tag "odgi/unchop" + + test("homo_sapiens - pangenome - pangenome_smoothxg_gfa") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['pangenome']['pangenome_smoothxg_gfa'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/odgi/unchop/tests/main.nf.test.snap b/modules/nf-core/odgi/unchop/tests/main.nf.test.snap new file mode 100644 index 00000000..3f5082b2 --- /dev/null +++ b/modules/nf-core/odgi/unchop/tests/main.nf.test.snap @@ -0,0 +1,33 @@ +{ + "homo_sapiens - pangenome - pangenome_smoothxg_gfa": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.og:md5,91a02c7320969d66bc862cdea317e92e" + ] + ], + "1": [ + "versions.yml:md5,721a9406e96a94ccb0ec44dc4f76ed09" + ], + "unchopped_graph": [ + [ + { + "id": "test", + "single_end": false + }, + "test.og:md5,91a02c7320969d66bc862cdea317e92e" + ] + ], + "versions": [ + "versions.yml:md5,721a9406e96a94ccb0ec44dc4f76ed09" + ] + } + ], + "timestamp": "2024-01-30T10:40:05.942092101" + } +} \ No newline at end of file diff --git a/modules/nf-core/odgi/view/environment.yml b/modules/nf-core/odgi/view/environment.yml new file mode 100644 index 00000000..94bf48f5 --- /dev/null +++ b/modules/nf-core/odgi/view/environment.yml @@ -0,0 +1,7 @@ +name: odgi_view +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::odgi=0.8.4 diff --git a/modules/nf-core/odgi/view/main.nf b/modules/nf-core/odgi/view/main.nf index cecfe454..83672e4e 100644 --- a/modules/nf-core/odgi/view/main.nf +++ b/modules/nf-core/odgi/view/main.nf @@ -2,10 +2,10 @@ process ODGI_VIEW { tag "$meta.id" label 'process_single' - conda "bioconda::odgi=0.8.3" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': - 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.4--py310h6cc9453_0': + 'biocontainers/odgi:0.8.4--py310h6cc9453_0' }" input: tuple val(meta), path(graph) diff --git a/modules/nf-core/odgi/view/meta.yml b/modules/nf-core/odgi/view/meta.yml index 68b1f17a..2b8b0d4d 100644 --- a/modules/nf-core/odgi/view/meta.yml +++ b/modules/nf-core/odgi/view/meta.yml @@ -12,8 +12,7 @@ tools: documentation: "https://odgi.readthedocs.io/en/latest/" tool_dev_url: "https://github.com/pangenome/odgi" doi: "10.1093/bioinformatics/btac308" - licence: "['MIT']" - + licence: ["MIT"] input: - meta: type: map @@ -24,7 +23,6 @@ input: type: file description: Pangenome graph in GFA v1.0 format or in ODGI binary format pattern: "*.{gfa,og}" - output: - meta: type: map @@ -39,6 +37,7 @@ output: type: file description: File containing a pangenome graph in GFA v1.0 format. pattern: "*.{gfa}" - authors: - "@subwaystation" +maintainers: + - "@subwaystation" diff --git a/modules/nf-core/odgi/view/tests/main.nf.test b/modules/nf-core/odgi/view/tests/main.nf.test new file mode 100644 index 00000000..534608aa --- /dev/null +++ b/modules/nf-core/odgi/view/tests/main.nf.test @@ -0,0 +1,34 @@ +nextflow_process { + + name "Test Process ODGI_VIEW" + script "../main.nf" + process "ODGI_VIEW" + + tag "modules" + tag "modules_nfcore" + tag "odgi" + tag "odgi/view" + + test("homo_sapiens - pangenome - pangenome_smoothxg_gfa") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['pangenome']['pangenome_smoothxg_gfa'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/odgi/view/tests/main.nf.test.snap b/modules/nf-core/odgi/view/tests/main.nf.test.snap new file mode 100644 index 00000000..1d9fc967 --- /dev/null +++ b/modules/nf-core/odgi/view/tests/main.nf.test.snap @@ -0,0 +1,33 @@ +{ + "homo_sapiens - pangenome - pangenome_smoothxg_gfa": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.gfa:md5,f6c3da9f9317b4546cbb74ad35bf8726" + ] + ], + "1": [ + "versions.yml:md5,cda909193ec196138daed6da151a8e83" + ], + "gfa": [ + [ + { + "id": "test", + "single_end": false + }, + "test.gfa:md5,f6c3da9f9317b4546cbb74ad35bf8726" + ] + ], + "versions": [ + "versions.yml:md5,cda909193ec196138daed6da151a8e83" + ] + } + ], + "timestamp": "2024-01-30T10:35:28.605315319" + } +} \ No newline at end of file diff --git a/modules/nf-core/odgi/viz/environment.yml b/modules/nf-core/odgi/viz/environment.yml new file mode 100644 index 00000000..8931e0ec --- /dev/null +++ b/modules/nf-core/odgi/viz/environment.yml @@ -0,0 +1,7 @@ +name: odgi_viz +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::odgi=0.8.4 diff --git a/modules/nf-core/odgi/viz/main.nf b/modules/nf-core/odgi/viz/main.nf index feef5be2..562300eb 100644 --- a/modules/nf-core/odgi/viz/main.nf +++ b/modules/nf-core/odgi/viz/main.nf @@ -2,10 +2,10 @@ process ODGI_VIZ { tag "$meta.id" label 'process_single' - conda "bioconda::odgi=0.8.3" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/odgi:0.8.3--py310h6cc9453_0': - 'biocontainers/odgi:0.8.3--py310h6cc9453_0' }" + 'https://depot.galaxyproject.org/singularity/odgi:0.8.4--py310h6cc9453_0': + 'biocontainers/odgi:0.8.4--py310h6cc9453_0' }" input: tuple val(meta), path(graph) diff --git a/modules/nf-core/odgi/viz/meta.yml b/modules/nf-core/odgi/viz/meta.yml index 66fa8798..8f8a0370 100644 --- a/modules/nf-core/odgi/viz/meta.yml +++ b/modules/nf-core/odgi/viz/meta.yml @@ -12,8 +12,7 @@ tools: documentation: "https://odgi.readthedocs.io/en/latest/" tool_dev_url: "https://github.com/pangenome/odgi" doi: "10.1093/bioinformatics/btac308" - licence: "['MIT']" - + licence: ["MIT"] input: - meta: type: map @@ -24,7 +23,6 @@ input: type: file description: Pangenome graph in binary ODGI or in GFA v1.0 format pattern: "*.{og,gfa}" - output: - meta: type: map @@ -39,6 +37,7 @@ output: type: file description: A 1D visualization of a pangenome graph. pattern: "*.{png}" - authors: - "@subwaystation" +maintainers: + - "@subwaystation" diff --git a/modules/nf-core/odgi/viz/tests/main.nf.test b/modules/nf-core/odgi/viz/tests/main.nf.test new file mode 100644 index 00000000..e6a2d058 --- /dev/null +++ b/modules/nf-core/odgi/viz/tests/main.nf.test @@ -0,0 +1,34 @@ +nextflow_process { + + name "Test Process ODGI_VIZ" + script "../main.nf" + process "ODGI_VIZ" + + tag "modules" + tag "modules_nfcore" + tag "odgi" + tag "odgi/viz" + + test("homo_sapiens - pangenome - pangenome_smoothxg_gfa") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['pangenome']['pangenome_smoothxg_gfa'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/odgi/viz/tests/main.nf.test.snap b/modules/nf-core/odgi/viz/tests/main.nf.test.snap new file mode 100644 index 00000000..49e909ba --- /dev/null +++ b/modules/nf-core/odgi/viz/tests/main.nf.test.snap @@ -0,0 +1,33 @@ +{ + "homo_sapiens - pangenome - pangenome_smoothxg_gfa": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.png:md5,45aad95d5e11a87c2f8c03ca703438bf" + ] + ], + "1": [ + "versions.yml:md5,95c0ee8015cf342c2724fb64d4cba67e" + ], + "png": [ + [ + { + "id": "test", + "single_end": false + }, + "test.png:md5,45aad95d5e11a87c2f8c03ca703438bf" + ] + ], + "versions": [ + "versions.yml:md5,95c0ee8015cf342c2724fb64d4cba67e" + ] + } + ], + "timestamp": "2024-01-30T09:53:51.373368285" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/faidx/environment.yml b/modules/nf-core/samtools/faidx/environment.yml new file mode 100644 index 00000000..4807ba5f --- /dev/null +++ b/modules/nf-core/samtools/faidx/environment.yml @@ -0,0 +1,8 @@ +name: samtools_faidx +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.18 + - bioconda::htslib=1.18 diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf index 59ed3088..d3461627 100644 --- a/modules/nf-core/samtools/faidx/main.nf +++ b/modules/nf-core/samtools/faidx/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_FAIDX { tag "$fasta" label 'process_single' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : + 'biocontainers/samtools:1.18--h50ea8bc_1' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/samtools/faidx/meta.yml b/modules/nf-core/samtools/faidx/meta.yml index 957b25e5..e189af28 100644 --- a/modules/nf-core/samtools/faidx/meta.yml +++ b/modules/nf-core/samtools/faidx/meta.yml @@ -55,3 +55,7 @@ authors: - "@drpatelh" - "@ewels" - "@phue" +maintainers: + - "@drpatelh" + - "@ewels" + - "@phue" diff --git a/modules/nf-core/seqwish/induce/environment.yml b/modules/nf-core/seqwish/induce/environment.yml new file mode 100644 index 00000000..63711c0b --- /dev/null +++ b/modules/nf-core/seqwish/induce/environment.yml @@ -0,0 +1,7 @@ +name: seqwish_induce +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::seqwish=0.7.9 diff --git a/modules/nf-core/seqwish/induce/main.nf b/modules/nf-core/seqwish/induce/main.nf index 2a6a8ba5..f4517f95 100644 --- a/modules/nf-core/seqwish/induce/main.nf +++ b/modules/nf-core/seqwish/induce/main.nf @@ -2,7 +2,7 @@ process SEQWISH_INDUCE { tag "$meta.id" label 'process_medium' - conda "bioconda::seqwish=0.7.9" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/seqwish:0.7.9--h5b5514e_0' : 'biocontainers/seqwish:0.7.9--h5b5514e_0' }" diff --git a/modules/nf-core/seqwish/induce/meta.yml b/modules/nf-core/seqwish/induce/meta.yml index f6b92236..562f4014 100644 --- a/modules/nf-core/seqwish/induce/meta.yml +++ b/modules/nf-core/seqwish/induce/meta.yml @@ -28,7 +28,6 @@ input: type: file description: FASTA file used to generate alignments pattern: "*.{fa,fa.gz,fasta,fasta.gz}" - output: - meta: type: map @@ -45,3 +44,5 @@ output: pattern: "versions.yml" authors: - "@heuermh" +maintainers: + - "@heuermh" diff --git a/modules/nf-core/smoothxg/environment.yml b/modules/nf-core/smoothxg/environment.yml new file mode 100644 index 00000000..76ca4b86 --- /dev/null +++ b/modules/nf-core/smoothxg/environment.yml @@ -0,0 +1,7 @@ +name: smoothxg +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::smoothxg=0.7.2 diff --git a/modules/nf-core/smoothxg/main.nf b/modules/nf-core/smoothxg/main.nf index 0d10a4a4..191e14cd 100644 --- a/modules/nf-core/smoothxg/main.nf +++ b/modules/nf-core/smoothxg/main.nf @@ -2,10 +2,10 @@ process SMOOTHXG { tag "$meta.id" label 'process_high' - conda "bioconda::smoothxg=0.7.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/smoothxg:0.7.1--h40c17d1_0' : - 'biocontainers/smoothxg:0.7.1--h40c17d1_0' }" + 'https://depot.galaxyproject.org/singularity/smoothxg:0.7.2--h40c17d1_0' : + 'biocontainers/smoothxg:0.7.2--h40c17d1_0' }" input: tuple val(meta), path(gfa) diff --git a/modules/nf-core/smoothxg/meta.yml b/modules/nf-core/smoothxg/meta.yml index d6a744f0..2ba721f8 100644 --- a/modules/nf-core/smoothxg/meta.yml +++ b/modules/nf-core/smoothxg/meta.yml @@ -24,7 +24,6 @@ input: type: file description: Variation graph in GFA 1.0 format pattern: "*.{gfa}" - output: - meta: type: map @@ -45,3 +44,5 @@ output: pattern: "*.{maf}" authors: - "@heuermh, @subwaystation" +maintainers: + - "@heuermh, @subwaystation" diff --git a/modules/nf-core/smoothxg/tests/main.nf.test b/modules/nf-core/smoothxg/tests/main.nf.test new file mode 100644 index 00000000..ed19da0b --- /dev/null +++ b/modules/nf-core/smoothxg/tests/main.nf.test @@ -0,0 +1,56 @@ +nextflow_process { + + name "Test Process SMOOTHXG" + script "../main.nf" + process "SMOOTHXG" + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "smoothxg" + + test("sarscov2 - illumina - assembly_gfa") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['assembly_gfa'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, +// { assert snapshot(process.out).match() } // SMOOTHXG is never deterministic + ) + } + + } + + test("homo_sapiens - pangenome - pangenome_seqwish_gfa") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['pangenome']['pangenome_seqwish_gfa'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, +// { assert snapshot(process.out).match() } // SMOOTHXG is never deterministic + ) + } + + } + +} diff --git a/modules/nf-core/smoothxg/tests/main.nf.test.snap b/modules/nf-core/smoothxg/tests/main.nf.test.snap new file mode 100644 index 00000000..34123620 --- /dev/null +++ b/modules/nf-core/smoothxg/tests/main.nf.test.snap @@ -0,0 +1,39 @@ +{ + "sarscov2 - illumina - assembly_gfa": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.smoothxg.gfa:md5,c85140490fb255f8361499c01d0dd15b" + ] + ], + "1": [ + "test.maf:md5,a6d82f572a5358926b87c69683d7825e" + ], + "2": [ + "versions.yml:md5,14c3d1a28b189a6db45facabbb5dc274" + ], + "gfa": [ + [ + { + "id": "test", + "single_end": false + }, + "test.smoothxg.gfa:md5,c85140490fb255f8361499c01d0dd15b" + ] + ], + "maf": [ + "test.maf:md5,a6d82f572a5358926b87c69683d7825e" + ], + "versions": [ + "versions.yml:md5,14c3d1a28b189a6db45facabbb5dc274" + ] + } + ], + "timestamp": "2024-01-25T16:06:35.857843316" + } +} \ No newline at end of file diff --git a/modules/nf-core/smoothxg/tests/nextflow.config b/modules/nf-core/smoothxg/tests/nextflow.config new file mode 100644 index 00000000..2d2b27d9 --- /dev/null +++ b/modules/nf-core/smoothxg/tests/nextflow.config @@ -0,0 +1,11 @@ +process { + + withName: SMOOTHXG { + ext.args = { + [ + "--write-msa-in-maf-format test.maf --n-haps 10" + ].join(" ").trim() + } + } + +} diff --git a/modules/nf-core/smoothxg/tests/tags.yml b/modules/nf-core/smoothxg/tests/tags.yml new file mode 100644 index 00000000..2089a657 --- /dev/null +++ b/modules/nf-core/smoothxg/tests/tags.yml @@ -0,0 +1,2 @@ +smoothxg: + - "modules/nf-core/smoothxg/**" diff --git a/modules/nf-core/tabix/bgzip/environment.yml b/modules/nf-core/tabix/bgzip/environment.yml new file mode 100644 index 00000000..4fe40c56 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/environment.yml @@ -0,0 +1,7 @@ +name: tabix_bgzip +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::tabix=1.11 diff --git a/modules/nf-core/tabix/bgzip/main.nf b/modules/nf-core/tabix/bgzip/main.nf index 8c47d9e2..7772e9ad 100644 --- a/modules/nf-core/tabix/bgzip/main.nf +++ b/modules/nf-core/tabix/bgzip/main.nf @@ -2,7 +2,7 @@ process TABIX_BGZIP { tag "$meta.id" label 'process_single' - conda "bioconda::tabix=1.11" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : 'biocontainers/tabix:1.11--hdfd78af_0' }" diff --git a/modules/nf-core/tabix/bgzip/meta.yml b/modules/nf-core/tabix/bgzip/meta.yml index c3ea2107..621d49ea 100644 --- a/modules/nf-core/tabix/bgzip/meta.yml +++ b/modules/nf-core/tabix/bgzip/meta.yml @@ -45,3 +45,8 @@ authors: - "@drpatelh" - "@maxulysse" - "@nvnieuwk" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@maxulysse" + - "@nvnieuwk" diff --git a/modules/nf-core/wfmash/environment.yml b/modules/nf-core/wfmash/environment.yml new file mode 100644 index 00000000..4e7e66c3 --- /dev/null +++ b/modules/nf-core/wfmash/environment.yml @@ -0,0 +1,7 @@ +name: wfmash +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::wfmash=0.12.5 diff --git a/modules/nf-core/wfmash/main.nf b/modules/nf-core/wfmash/main.nf index 0f9c5818..3e1592f4 100644 --- a/modules/nf-core/wfmash/main.nf +++ b/modules/nf-core/wfmash/main.nf @@ -2,10 +2,10 @@ process WFMASH { tag "$meta.id" label 'process_medium' - conda "bioconda::wfmash=0.10.5" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/wfmash:0.10.5--h94f6cfe_0': - 'biocontainers/wfmash:0.10.5--h94f6cfe_0' }" + 'https://depot.galaxyproject.org/singularity/wfmash:0.12.5--h11f254b_0': + 'biocontainers/wfmash:0.12.5--h11f254b_0' }" input: tuple val(meta), path(fasta_gz), path(paf), path(gzi), path(fai) diff --git a/modules/nf-core/wfmash/meta.yml b/modules/nf-core/wfmash/meta.yml index 80e630cc..dc5cd5a8 100644 --- a/modules/nf-core/wfmash/meta.yml +++ b/modules/nf-core/wfmash/meta.yml @@ -13,8 +13,7 @@ tools: documentation: https://github.com/waveygang/wfmash tool_dev_url: https://github.com/waveygang/wfmash doi: 10.5281/zenodo.6949373 - licence: "['MIT']" - + licence: ["MIT"] input: - meta: type: map @@ -44,7 +43,6 @@ input: type: file description: Optional inpute file in FASTA format specifying the query sequences as a list. pattern: "*.{fa,fna,fasta}" - output: - meta: type: map @@ -59,6 +57,7 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@subwaystation" +maintainers: + - "@subwaystation" diff --git a/modules/nf-core/wfmash/tests/main.nf.test b/modules/nf-core/wfmash/tests/main.nf.test new file mode 100644 index 00000000..41d8c23c --- /dev/null +++ b/modules/nf-core/wfmash/tests/main.nf.test @@ -0,0 +1,39 @@ +nextflow_process { + + name "Test Process WFMASH" + script "../main.nf" + process "WFMASH" + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "wfmash" + + test("homo_sapiens - pangenome - pangenome_fa_bgzip") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['pangenome']['pangenome_fa_bgzip'], checkIfExists: true), + [], // empty paf input + file(params.test_data['homo_sapiens']['pangenome']['pangenome_fa_bgzip_gzi'], checkIfExists: true), // gzi + file(params.test_data['homo_sapiens']['pangenome']['pangenome_fa_bgzip_fai'], checkIfExists: true) // fai + ] + input[1] = true // empty paf input + input[2] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, +// { assert snapshot(process.out).exists() } // if run on more than 1 thread the lines in the resulting PAF will appear in a different order + ) + } + + } + +} diff --git a/modules/nf-core/wfmash/tests/main.nf.test.snap b/modules/nf-core/wfmash/tests/main.nf.test.snap new file mode 100644 index 00000000..97a51e44 --- /dev/null +++ b/modules/nf-core/wfmash/tests/main.nf.test.snap @@ -0,0 +1,33 @@ +{ + "homo_sapiens - pangenome - pangenome_fa_bgzip": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.paf:md5,aa6629548e32b2d036c0767c9086a6dd" + ] + ], + "1": [ + "versions.yml:md5,0da45b7a6424a967e2f39225fb020140" + ], + "paf": [ + [ + { + "id": "test", + "single_end": false + }, + "test.paf:md5,aa6629548e32b2d036c0767c9086a6dd" + ] + ], + "versions": [ + "versions.yml:md5,0da45b7a6424a967e2f39225fb020140" + ] + } + ], + "timestamp": "2024-01-25T15:12:21.284828212" + } +} \ No newline at end of file diff --git a/modules/nf-core/wfmash/tests/nextflow.config b/modules/nf-core/wfmash/tests/nextflow.config new file mode 100644 index 00000000..b7e9c989 --- /dev/null +++ b/modules/nf-core/wfmash/tests/nextflow.config @@ -0,0 +1,12 @@ +process { + + withName: WFMASH { + ext.args = { + [ + "-s 500", // necessary because of https://github.com/waveygang/wfmash/issues/218 + "-X" + ].join(" ").trim() + } + } + +} diff --git a/modules/nf-core/wfmash/tests/tags.yml b/modules/nf-core/wfmash/tests/tags.yml new file mode 100644 index 00000000..7001b087 --- /dev/null +++ b/modules/nf-core/wfmash/tests/tags.yml @@ -0,0 +1,2 @@ +wfmash: + - "modules/nf-core/wfmash/**" From ef4996583e8179c8abe357296206a830f8339191 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Thu, 1 Feb 2024 15:39:56 +0100 Subject: [PATCH 43/83] revert wfmash to 0.10.5 --- modules.json | 2 +- modules/nf-core/wfmash/main.nf | 6 +++--- modules/nf-core/wfmash/meta.yml | 4 +--- 3 files changed, 5 insertions(+), 7 deletions(-) diff --git a/modules.json b/modules.json index 7b0774ba..fb1833c0 100644 --- a/modules.json +++ b/modules.json @@ -87,7 +87,7 @@ }, "wfmash": { "branch": "master", - "git_sha": "8cbeaa5063f08952682517597d4e8d6e35bbcd1a", + "git_sha": "96fadda088c6e79777ba8eef939e36bb03a69c65", "installed_by": ["modules"] } } diff --git a/modules/nf-core/wfmash/main.nf b/modules/nf-core/wfmash/main.nf index 3e1592f4..0f9c5818 100644 --- a/modules/nf-core/wfmash/main.nf +++ b/modules/nf-core/wfmash/main.nf @@ -2,10 +2,10 @@ process WFMASH { tag "$meta.id" label 'process_medium' - conda "${moduleDir}/environment.yml" + conda "bioconda::wfmash=0.10.5" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/wfmash:0.12.5--h11f254b_0': - 'biocontainers/wfmash:0.12.5--h11f254b_0' }" + 'https://depot.galaxyproject.org/singularity/wfmash:0.10.5--h94f6cfe_0': + 'biocontainers/wfmash:0.10.5--h94f6cfe_0' }" input: tuple val(meta), path(fasta_gz), path(paf), path(gzi), path(fai) diff --git a/modules/nf-core/wfmash/meta.yml b/modules/nf-core/wfmash/meta.yml index dc5cd5a8..1063f8b5 100644 --- a/modules/nf-core/wfmash/meta.yml +++ b/modules/nf-core/wfmash/meta.yml @@ -13,7 +13,7 @@ tools: documentation: https://github.com/waveygang/wfmash tool_dev_url: https://github.com/waveygang/wfmash doi: 10.5281/zenodo.6949373 - licence: ["MIT"] + licence: "['MIT']" input: - meta: type: map @@ -59,5 +59,3 @@ output: pattern: "versions.yml" authors: - "@subwaystation" -maintainers: - - "@subwaystation" From a78db057ee409865b358eacbecff5da398134a39 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Thu, 1 Feb 2024 15:57:53 +0100 Subject: [PATCH 44/83] now --- modules/nf-core/wfmash/environment.yml | 7 ---- modules/nf-core/wfmash/meta.yml | 3 ++ modules/nf-core/wfmash/tests/main.nf.test | 39 ------------------- .../nf-core/wfmash/tests/main.nf.test.snap | 33 ---------------- modules/nf-core/wfmash/tests/nextflow.config | 12 ------ modules/nf-core/wfmash/tests/tags.yml | 2 - 6 files changed, 3 insertions(+), 93 deletions(-) delete mode 100644 modules/nf-core/wfmash/environment.yml delete mode 100644 modules/nf-core/wfmash/tests/main.nf.test delete mode 100644 modules/nf-core/wfmash/tests/main.nf.test.snap delete mode 100644 modules/nf-core/wfmash/tests/nextflow.config delete mode 100644 modules/nf-core/wfmash/tests/tags.yml diff --git a/modules/nf-core/wfmash/environment.yml b/modules/nf-core/wfmash/environment.yml deleted file mode 100644 index 4e7e66c3..00000000 --- a/modules/nf-core/wfmash/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: wfmash -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::wfmash=0.12.5 diff --git a/modules/nf-core/wfmash/meta.yml b/modules/nf-core/wfmash/meta.yml index 1063f8b5..80e630cc 100644 --- a/modules/nf-core/wfmash/meta.yml +++ b/modules/nf-core/wfmash/meta.yml @@ -14,6 +14,7 @@ tools: tool_dev_url: https://github.com/waveygang/wfmash doi: 10.5281/zenodo.6949373 licence: "['MIT']" + input: - meta: type: map @@ -43,6 +44,7 @@ input: type: file description: Optional inpute file in FASTA format specifying the query sequences as a list. pattern: "*.{fa,fna,fasta}" + output: - meta: type: map @@ -57,5 +59,6 @@ output: type: file description: File containing software versions pattern: "versions.yml" + authors: - "@subwaystation" diff --git a/modules/nf-core/wfmash/tests/main.nf.test b/modules/nf-core/wfmash/tests/main.nf.test deleted file mode 100644 index 41d8c23c..00000000 --- a/modules/nf-core/wfmash/tests/main.nf.test +++ /dev/null @@ -1,39 +0,0 @@ -nextflow_process { - - name "Test Process WFMASH" - script "../main.nf" - process "WFMASH" - config "./nextflow.config" - - tag "modules" - tag "modules_nfcore" - tag "wfmash" - - test("homo_sapiens - pangenome - pangenome_fa_bgzip") { - - when { - process { - """ - input[0] = [ - [ id:'test', single_end:false ], // meta map - file(params.test_data['homo_sapiens']['pangenome']['pangenome_fa_bgzip'], checkIfExists: true), - [], // empty paf input - file(params.test_data['homo_sapiens']['pangenome']['pangenome_fa_bgzip_gzi'], checkIfExists: true), // gzi - file(params.test_data['homo_sapiens']['pangenome']['pangenome_fa_bgzip_fai'], checkIfExists: true) // fai - ] - input[1] = true // empty paf input - input[2] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, -// { assert snapshot(process.out).exists() } // if run on more than 1 thread the lines in the resulting PAF will appear in a different order - ) - } - - } - -} diff --git a/modules/nf-core/wfmash/tests/main.nf.test.snap b/modules/nf-core/wfmash/tests/main.nf.test.snap deleted file mode 100644 index 97a51e44..00000000 --- a/modules/nf-core/wfmash/tests/main.nf.test.snap +++ /dev/null @@ -1,33 +0,0 @@ -{ - "homo_sapiens - pangenome - pangenome_fa_bgzip": { - "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": false - }, - "test.paf:md5,aa6629548e32b2d036c0767c9086a6dd" - ] - ], - "1": [ - "versions.yml:md5,0da45b7a6424a967e2f39225fb020140" - ], - "paf": [ - [ - { - "id": "test", - "single_end": false - }, - "test.paf:md5,aa6629548e32b2d036c0767c9086a6dd" - ] - ], - "versions": [ - "versions.yml:md5,0da45b7a6424a967e2f39225fb020140" - ] - } - ], - "timestamp": "2024-01-25T15:12:21.284828212" - } -} \ No newline at end of file diff --git a/modules/nf-core/wfmash/tests/nextflow.config b/modules/nf-core/wfmash/tests/nextflow.config deleted file mode 100644 index b7e9c989..00000000 --- a/modules/nf-core/wfmash/tests/nextflow.config +++ /dev/null @@ -1,12 +0,0 @@ -process { - - withName: WFMASH { - ext.args = { - [ - "-s 500", // necessary because of https://github.com/waveygang/wfmash/issues/218 - "-X" - ].join(" ").trim() - } - } - -} diff --git a/modules/nf-core/wfmash/tests/tags.yml b/modules/nf-core/wfmash/tests/tags.yml deleted file mode 100644 index 7001b087..00000000 --- a/modules/nf-core/wfmash/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -wfmash: - - "modules/nf-core/wfmash/**" From c621412d6c70e9407f718cba30bc4db7af47f9f2 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Wed, 7 Feb 2024 15:43:33 +0100 Subject: [PATCH 45/83] update wfmash, multiqc --- modules.json | 4 +- modules/nf-core/multiqc/tests/main.nf.test | 13 ++++--- .../nf-core/multiqc/tests/main.nf.test.snap | 28 +++++++++++-- modules/nf-core/wfmash/environment.yml | 7 ++++ modules/nf-core/wfmash/main.nf | 6 +-- modules/nf-core/wfmash/meta.yml | 7 ++-- modules/nf-core/wfmash/tests/main.nf.test | 39 +++++++++++++++++++ .../nf-core/wfmash/tests/main.nf.test.snap | 33 ++++++++++++++++ modules/nf-core/wfmash/tests/nextflow.config | 12 ++++++ modules/nf-core/wfmash/tests/tags.yml | 2 + 10 files changed, 132 insertions(+), 19 deletions(-) create mode 100644 modules/nf-core/wfmash/environment.yml create mode 100644 modules/nf-core/wfmash/tests/main.nf.test create mode 100644 modules/nf-core/wfmash/tests/main.nf.test.snap create mode 100644 modules/nf-core/wfmash/tests/nextflow.config create mode 100644 modules/nf-core/wfmash/tests/tags.yml diff --git a/modules.json b/modules.json index fb1833c0..07b78a01 100644 --- a/modules.json +++ b/modules.json @@ -17,7 +17,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", + "git_sha": "9e71d8519dfbfc328c078bba14d4bd4c99e39a94", "installed_by": ["modules"] }, "odgi/build": { @@ -87,7 +87,7 @@ }, "wfmash": { "branch": "master", - "git_sha": "96fadda088c6e79777ba8eef939e36bb03a69c65", + "git_sha": "0c49f318ec4701b891a87f25fb07b34713c90203", "installed_by": ["modules"] } } diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index d0438eda..f1c4242e 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -3,6 +3,7 @@ nextflow_process { name "Test Process MULTIQC" script "../main.nf" process "MULTIQC" + tag "modules" tag "modules_nfcore" tag "multiqc" @@ -12,7 +13,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -25,7 +26,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_single") } ) } @@ -36,7 +37,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] @@ -49,7 +50,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_config") } ) } } @@ -61,7 +62,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -75,7 +76,7 @@ nextflow_process { { assert snapshot(process.out.report.collect { file(it).getName() } + process.out.data.collect { file(it).getName() } + process.out.plots.collect { file(it).getName() } + - process.out.versions ).match() } + process.out.versions ).match("multiqc_stub") } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index d37e7304..549ba79c 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -1,13 +1,17 @@ { - "versions": { + "multiqc_versions_single": { "content": [ [ "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" ] ], - "timestamp": "2024-01-09T23:02:49.911994" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:43:40.529579" }, - "sarscov2 single-end [fastqc] - stub": { + "multiqc_stub": { "content": [ [ "multiqc_report.html", @@ -16,6 +20,22 @@ "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" ] ], - "timestamp": "2024-01-09T23:03:14.524346" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:45:09.605359" + }, + "multiqc_versions_config": { + "content": [ + [ + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:44:53.535994" } } \ No newline at end of file diff --git a/modules/nf-core/wfmash/environment.yml b/modules/nf-core/wfmash/environment.yml new file mode 100644 index 00000000..071aa5a7 --- /dev/null +++ b/modules/nf-core/wfmash/environment.yml @@ -0,0 +1,7 @@ +name: wfmash +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::wfmash=0.12.6 diff --git a/modules/nf-core/wfmash/main.nf b/modules/nf-core/wfmash/main.nf index 0f9c5818..7c64148b 100644 --- a/modules/nf-core/wfmash/main.nf +++ b/modules/nf-core/wfmash/main.nf @@ -2,10 +2,10 @@ process WFMASH { tag "$meta.id" label 'process_medium' - conda "bioconda::wfmash=0.10.5" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/wfmash:0.10.5--h94f6cfe_0': - 'biocontainers/wfmash:0.10.5--h94f6cfe_0' }" + 'https://depot.galaxyproject.org/singularity/wfmash:0.12.6--h11f254b_0': + 'biocontainers/wfmash:0.12.6--h11f254b_0' }" input: tuple val(meta), path(fasta_gz), path(paf), path(gzi), path(fai) diff --git a/modules/nf-core/wfmash/meta.yml b/modules/nf-core/wfmash/meta.yml index 80e630cc..dc5cd5a8 100644 --- a/modules/nf-core/wfmash/meta.yml +++ b/modules/nf-core/wfmash/meta.yml @@ -13,8 +13,7 @@ tools: documentation: https://github.com/waveygang/wfmash tool_dev_url: https://github.com/waveygang/wfmash doi: 10.5281/zenodo.6949373 - licence: "['MIT']" - + licence: ["MIT"] input: - meta: type: map @@ -44,7 +43,6 @@ input: type: file description: Optional inpute file in FASTA format specifying the query sequences as a list. pattern: "*.{fa,fna,fasta}" - output: - meta: type: map @@ -59,6 +57,7 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@subwaystation" +maintainers: + - "@subwaystation" diff --git a/modules/nf-core/wfmash/tests/main.nf.test b/modules/nf-core/wfmash/tests/main.nf.test new file mode 100644 index 00000000..41d8c23c --- /dev/null +++ b/modules/nf-core/wfmash/tests/main.nf.test @@ -0,0 +1,39 @@ +nextflow_process { + + name "Test Process WFMASH" + script "../main.nf" + process "WFMASH" + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "wfmash" + + test("homo_sapiens - pangenome - pangenome_fa_bgzip") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['pangenome']['pangenome_fa_bgzip'], checkIfExists: true), + [], // empty paf input + file(params.test_data['homo_sapiens']['pangenome']['pangenome_fa_bgzip_gzi'], checkIfExists: true), // gzi + file(params.test_data['homo_sapiens']['pangenome']['pangenome_fa_bgzip_fai'], checkIfExists: true) // fai + ] + input[1] = true // empty paf input + input[2] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, +// { assert snapshot(process.out).exists() } // if run on more than 1 thread the lines in the resulting PAF will appear in a different order + ) + } + + } + +} diff --git a/modules/nf-core/wfmash/tests/main.nf.test.snap b/modules/nf-core/wfmash/tests/main.nf.test.snap new file mode 100644 index 00000000..97a51e44 --- /dev/null +++ b/modules/nf-core/wfmash/tests/main.nf.test.snap @@ -0,0 +1,33 @@ +{ + "homo_sapiens - pangenome - pangenome_fa_bgzip": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.paf:md5,aa6629548e32b2d036c0767c9086a6dd" + ] + ], + "1": [ + "versions.yml:md5,0da45b7a6424a967e2f39225fb020140" + ], + "paf": [ + [ + { + "id": "test", + "single_end": false + }, + "test.paf:md5,aa6629548e32b2d036c0767c9086a6dd" + ] + ], + "versions": [ + "versions.yml:md5,0da45b7a6424a967e2f39225fb020140" + ] + } + ], + "timestamp": "2024-01-25T15:12:21.284828212" + } +} \ No newline at end of file diff --git a/modules/nf-core/wfmash/tests/nextflow.config b/modules/nf-core/wfmash/tests/nextflow.config new file mode 100644 index 00000000..b7e9c989 --- /dev/null +++ b/modules/nf-core/wfmash/tests/nextflow.config @@ -0,0 +1,12 @@ +process { + + withName: WFMASH { + ext.args = { + [ + "-s 500", // necessary because of https://github.com/waveygang/wfmash/issues/218 + "-X" + ].join(" ").trim() + } + } + +} diff --git a/modules/nf-core/wfmash/tests/tags.yml b/modules/nf-core/wfmash/tests/tags.yml new file mode 100644 index 00000000..7001b087 --- /dev/null +++ b/modules/nf-core/wfmash/tests/tags.yml @@ -0,0 +1,2 @@ +wfmash: + - "modules/nf-core/wfmash/**" From 59b0113f5517ab7f71541a45e8fdd652f5e9f423 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Wed, 7 Feb 2024 15:51:31 +0100 Subject: [PATCH 46/83] bug fix --- modules/local/split_approx_mappings_in_chunks/main.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/modules/local/split_approx_mappings_in_chunks/main.nf b/modules/local/split_approx_mappings_in_chunks/main.nf index 3d61ab5a..2cd3c765 100644 --- a/modules/local/split_approx_mappings_in_chunks/main.nf +++ b/modules/local/split_approx_mappings_in_chunks/main.nf @@ -2,10 +2,10 @@ process SPLIT_APPROX_MAPPINGS_IN_CHUNKS { tag "$meta.id" label 'process_single' - conda "bioconda::pggb=0.5.4" + conda "bioconda::wfmash=0.12.6" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/pggb:0.5.4--hdfd78af_0': - 'biocontainers/pggb:0.5.4--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/wfmash:0.12.6--h11f254b_0': + 'biocontainers/wfmash:0.12.6--h11f254b_0' }" input: tuple val(meta), path(paf) From e41a5256afc3601c505e8bf973066c520e9a8a7d Mon Sep 17 00:00:00 2001 From: subwaystation Date: Wed, 7 Feb 2024 16:00:45 +0100 Subject: [PATCH 47/83] another try --- modules/local/split_approx_mappings_in_chunks/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/split_approx_mappings_in_chunks/main.nf b/modules/local/split_approx_mappings_in_chunks/main.nf index 2cd3c765..ad573b02 100644 --- a/modules/local/split_approx_mappings_in_chunks/main.nf +++ b/modules/local/split_approx_mappings_in_chunks/main.nf @@ -21,7 +21,7 @@ process SPLIT_APPROX_MAPPINGS_IN_CHUNKS { def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" """ - split_approx_mappings_in_chunks.py $paf \\ + python split_approx_mappings_in_chunks.py $paf \\ $args cat <<-END_VERSIONS > versions.yml From c873b108abb782f2752b56567f4a166c56113b00 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Thu, 8 Feb 2024 11:45:51 +0100 Subject: [PATCH 48/83] another wfmash update --- modules/local/split_approx_mappings_in_chunks/main.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/split_approx_mappings_in_chunks/main.nf b/modules/local/split_approx_mappings_in_chunks/main.nf index ad573b02..878afe76 100644 --- a/modules/local/split_approx_mappings_in_chunks/main.nf +++ b/modules/local/split_approx_mappings_in_chunks/main.nf @@ -4,8 +4,8 @@ process SPLIT_APPROX_MAPPINGS_IN_CHUNKS { conda "bioconda::wfmash=0.12.6" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/wfmash:0.12.6--h11f254b_0': - 'biocontainers/wfmash:0.12.6--h11f254b_0' }" + 'https://depot.galaxyproject.org/singularity/wfmash:0.12.6--h11f254b_1': + 'biocontainers/wfmash:0.12.6--h11f254b_1' }" input: tuple val(meta), path(paf) From e3bd04c02a2b111fc90ca7aa72d4291d8c64e40b Mon Sep 17 00:00:00 2001 From: subwaystation Date: Thu, 8 Feb 2024 11:54:23 +0100 Subject: [PATCH 49/83] another wfmash update --- modules/local/split_approx_mappings_in_chunks/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/split_approx_mappings_in_chunks/main.nf b/modules/local/split_approx_mappings_in_chunks/main.nf index 878afe76..66f7e1ff 100644 --- a/modules/local/split_approx_mappings_in_chunks/main.nf +++ b/modules/local/split_approx_mappings_in_chunks/main.nf @@ -21,7 +21,7 @@ process SPLIT_APPROX_MAPPINGS_IN_CHUNKS { def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" """ - python split_approx_mappings_in_chunks.py $paf \\ + split_approx_mappings_in_chunks.py $paf \\ $args cat <<-END_VERSIONS > versions.yml From 28e73b153282c5f9467bc158af517a9b98c20a85 Mon Sep 17 00:00:00 2001 From: Adam Talbot <12817534+adamrtalbot@users.noreply.github.com> Date: Thu, 8 Feb 2024 12:05:42 +0000 Subject: [PATCH 50/83] Put functions within closure to evaluate them properly. --- conf/modules.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 24d01283..f83fd983 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -29,7 +29,7 @@ def generate_wfmash_sparse_map_cmd() { return wfmash_sparse_map_cmd } -def wfmash_sparse_map_cmd = generate_wfmash_sparse_map_cmd() +def wfmash_sparse_map_cmd = { generate_wfmash_sparse_map_cmd() } def generate_smoothxg_poa_params_cmd() { def smoothxg_poa_params_cmd = "-P ${params.smoothxg_poa_params}" @@ -45,7 +45,7 @@ def generate_smoothxg_poa_params_cmd() { return smoothxg_poa_params_cmd } -def smoothxg_poa_params_cmd = generate_smoothxg_poa_params_cmd() +def smoothxg_poa_params_cmd = { generate_smoothxg_poa_params_cmd() } def parse_int(int plain_integer) { return plain_integer From fe5c1c996a273cf59a98dfab3704c40b10e9cd05 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 20 Feb 2024 16:09:06 +0100 Subject: [PATCH 51/83] fix delim bug --- conf/modules.config | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index f83fd983..f9bd2857 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -106,7 +106,7 @@ process { "-s ${parse_int(params.wfmash_segment_length)}", "-p ${params.wfmash_map_pct_id}", params.wfmash_merge_segments ? "-M" : "", - params.wfmash_exclude_delim ? "-Y ${params.wfmash_exclude_delim}" : "-X", + params.wfmash_exclude_delim ? "-Y \"${params.wfmash_exclude_delim}\"" : "-X", params.wfmash_no_splits ? "-N" : "", params.wfmash_block_length ? "-l ${parse_int(params.wfmash_block_length)}" : "-l ${parse_int(params.wfmash_segment_length) * 5}", "-k ${params.wfmash_mash_kmer}", @@ -129,7 +129,7 @@ process { "-s ${parse_int(params.wfmash_segment_length)}", "-p ${params.wfmash_map_pct_id}", params.wfmash_merge_segments ? "-M" : "", - params.wfmash_exclude_delim ? "-Y ${params.wfmash_exclude_delim}" : "-X", + params.wfmash_exclude_delim ? "-Y \"${params.wfmash_exclude_delim}\"" : "-X", params.wfmash_no_splits ? "-N" : "", params.wfmash_block_length ? "-l ${parse_int(params.wfmash_block_length)}" : "-l ${parse_int(params.wfmash_segment_length) * 5}", "-k ${params.wfmash_mash_kmer}", @@ -153,7 +153,7 @@ process { "-s ${parse_int(params.wfmash_segment_length)}", "-p ${params.wfmash_map_pct_id}", params.wfmash_merge_segments ? "-M" : "", - params.wfmash_exclude_delim ? "-Y ${params.wfmash_exclude_delim}" : "-X", + params.wfmash_exclude_delim ? "-Y \"${params.wfmash_exclude_delim}\"" : "-X", params.wfmash_no_splits ? "-N" : "", params.wfmash_block_length ? "-l ${parse_int(params.wfmash_block_length)}" : "-l ${parse_int(params.wfmash_segment_length) * 5}", "-k ${params.wfmash_mash_kmer}", @@ -186,7 +186,7 @@ process { "-s ${parse_int(params.wfmash_segment_length)}", "-p ${params.wfmash_map_pct_id}", params.wfmash_merge_segments ? "-M" : "", - params.wfmash_exclude_delim ? "-Y ${params.wfmash_exclude_delim}" : "-X", + params.wfmash_exclude_delim ? "-Y \"${params.wfmash_exclude_delim}\"" : "-X", params.wfmash_no_splits ? "-N" : "", params.wfmash_block_length ? "-l ${parse_int(params.wfmash_block_length)}" : "-l ${parse_int(params.wfmash_segment_length) * 5}", "-k ${params.wfmash_mash_kmer}", From ccb1668e2d1da17858b1a001befb1a86666d008e Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 20 Feb 2024 15:29:03 +0000 Subject: [PATCH 52/83] Template update for nf-core/tools version 2.13 --- .editorconfig | 9 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/branch.yml | 2 +- .github/workflows/ci.yml | 7 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 17 +- .github/workflows/linting.yml | 12 +- .github/workflows/linting_comment.yml | 4 +- .github/workflows/release-announcements.yml | 11 +- README.md | 5 +- assets/multiqc_config.yml | 2 + assets/schema_input.json | 21 +- bin/check_samplesheet.py | 259 ----------- conf/modules.config | 8 - lib/NfcoreTemplate.groovy | 356 -------------- lib/Utils.groovy | 47 -- lib/WorkflowMain.groovy | 77 --- lib/WorkflowPangenome.groovy | 122 ----- main.nf | 100 ++-- modules.json | 28 +- modules/local/samplesheet_check.nf | 31 -- .../dumpsoftwareversions/environment.yml | 7 - .../custom/dumpsoftwareversions/main.nf | 24 - .../custom/dumpsoftwareversions/meta.yml | 37 -- .../templates/dumpsoftwareversions.py | 102 ---- .../dumpsoftwareversions/tests/main.nf.test | 43 -- .../tests/main.nf.test.snap | 33 -- .../dumpsoftwareversions/tests/tags.yml | 2 - modules/nf-core/fastqc/tests/main.nf.test | 14 +- .../nf-core/fastqc/tests/main.nf.test.snap | 76 ++- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- modules/nf-core/multiqc/tests/main.nf.test | 13 +- .../nf-core/multiqc/tests/main.nf.test.snap | 32 +- nextflow.config | 5 +- nextflow_schema.json | 1 + pyproject.toml | 8 +- subworkflows/local/input_check.nf | 44 -- .../utils_nfcore_pangenome_pipeline/main.nf | 247 ++++++++++ .../nf-core/utils_nextflow_pipeline/main.nf | 126 +++++ .../nf-core/utils_nextflow_pipeline/meta.yml | 38 ++ .../tests/main.function.nf.test | 54 +++ .../tests/main.function.nf.test.snap | 12 + .../tests/main.workflow.nf.test | 123 +++++ .../tests/nextflow.config | 9 + .../utils_nextflow_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfcore_pipeline/main.nf | 440 ++++++++++++++++++ .../nf-core/utils_nfcore_pipeline/meta.yml | 24 + .../tests/main.function.nf.test | 134 ++++++ .../tests/main.function.nf.test.snap | 138 ++++++ .../tests/main.workflow.nf.test | 29 ++ .../tests/main.workflow.nf.test.snap | 15 + .../tests/nextflow.config | 9 + .../utils_nfcore_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfvalidation_plugin/main.nf | 62 +++ .../utils_nfvalidation_plugin/meta.yml | 44 ++ .../tests/main.nf.test | 200 ++++++++ .../tests/nextflow_schema.json | 96 ++++ .../utils_nfvalidation_plugin/tests/tags.yml | 2 + workflows/pangenome.nf | 133 ++---- 61 files changed, 2108 insertions(+), 1406 deletions(-) delete mode 100755 bin/check_samplesheet.py delete mode 100755 lib/NfcoreTemplate.groovy delete mode 100644 lib/Utils.groovy delete mode 100755 lib/WorkflowMain.groovy delete mode 100755 lib/WorkflowPangenome.groovy delete mode 100644 modules/local/samplesheet_check.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml delete mode 100644 subworkflows/local/input_check.nf create mode 100644 subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/.editorconfig b/.editorconfig index 9b990088..dd9ffa53 100644 --- a/.editorconfig +++ b/.editorconfig @@ -18,7 +18,12 @@ end_of_line = unset insert_final_newline = unset trim_trailing_whitespace = unset indent_style = unset -indent_size = unset +[/subworkflows/nf-core/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset [/assets/email*] indent_size = unset @@ -28,5 +33,5 @@ indent_size = unset indent_style = unset # ignore python -[*.{py}] +[*.{py,md}] indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 9695be11..7f891f43 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 8ee95d63..bd5d1bc8 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 64f6a2d1..bf6df0cd 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v2 + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index be09b5d8..8fec3877 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,13 +28,16 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 with: version: "${{ matrix.NXF_VER }}" + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - name: Run pipeline with test data # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index e37cfda5..0b6b1f27 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v9 + - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458a..f823210d 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -6,6 +6,11 @@ name: Test successful pipeline download with 'nf-core download' # - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. on: workflow_dispatch: + inputs: + testbranch: + description: "The specific branch you wish to utilize for the test execution of nf-core download." + required: true + default: "dev" pull_request: types: - opened @@ -23,13 +28,13 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" - - uses: eWaterCycle/setup-singularity@v7 + - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: singularity-version: 3.8.3 @@ -42,13 +47,13 @@ jobs: run: | echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} - echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} - name: Download the pipeline env: NXF_SINGULARITY_CACHEDIR: ./ run: | - nf-core download ${{ env.REPO_LOWERCASE }} \ + nf-core download ${{ env.REPO_LOWERCASE }} \ --revision ${{ env.REPO_BRANCH }} \ --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ --compress "none" \ @@ -64,4 +69,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 81cd098e..748b4311 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,10 +14,10 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Set up Python 3.11 - uses: actions/setup-python@v5 + uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: 3.11 cache: "pip" @@ -32,12 +32,12 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" @@ -60,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v4 + uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 147bcd10..b706875f 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v3 + uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 with: workflow: linting.yml workflow_conclusion: completed @@ -21,7 +21,7 @@ jobs: run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@v2 + uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 21ac3f06..c3674af2 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -9,6 +9,11 @@ jobs: toot: runs-on: ubuntu-latest steps: + - name: get topics and convert to hashtags + id: get_topics + run: | + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + - uses: rzr/fediverse-action@master with: access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} @@ -20,11 +25,13 @@ jobs: Please see the changelog: ${{ github.event.release.html_url }} + ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics + send-tweet: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +63,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.1.0 + - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/README.md b/README.md index a51b5b53..3fa46acc 100644 --- a/README.md +++ b/README.md @@ -4,8 +4,9 @@ nf-core/pangenome -[![GitHub Actions CI Status](https://github.com/nf-core/pangenome/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/pangenome/actions?query=workflow%3A%22nf-core+CI%22) -[![GitHub Actions Linting Status](https://github.com/nf-core/pangenome/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/pangenome/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/pangenome/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) + +[![GitHub Actions CI Status](https://github.com/nf-core/pangenome/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/pangenome/actions/workflows/ci.yml) +[![GitHub Actions Linting Status](https://github.com/nf-core/pangenome/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/pangenome/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/pangenome/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 8173f6d7..c44a0180 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -11,3 +11,5 @@ report_section_order: order: -1002 export_plots: true + +disable_version_detection: true diff --git a/assets/schema_input.json b/assets/schema_input.json index 89256bdf..596a8b62 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -10,25 +10,22 @@ "sample": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] }, "fastq_1": { "type": "string", + "format": "file-path", + "exists": true, "pattern": "^\\S+\\.f(ast)?q\\.gz$", "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" }, "fastq_2": { - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", - "anyOf": [ - { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$" - }, - { - "type": "string", - "maxLength": 0 - } - ] + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.f(ast)?q\\.gz$", + "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" } }, "required": ["sample", "fastq_1"] diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index 4a758fe0..00000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,259 +0,0 @@ -#!/usr/bin/env python - - -"""Provide a command line tool to validate and transform tabular samplesheets.""" - - -import argparse -import csv -import logging -import sys -from collections import Counter -from pathlib import Path - -logger = logging.getLogger() - - -class RowChecker: - """ - Define a service that can validate and transform each given row. - - Attributes: - modified (list): A list of dicts, where each dict corresponds to a previously - validated and transformed row. The order of rows is maintained. - - """ - - VALID_FORMATS = ( - ".fq.gz", - ".fastq.gz", - ) - - def __init__( - self, - sample_col="sample", - first_col="fastq_1", - second_col="fastq_2", - single_col="single_end", - **kwargs, - ): - """ - Initialize the row checker with the expected column names. - - Args: - sample_col (str): The name of the column that contains the sample name - (default "sample"). - first_col (str): The name of the column that contains the first (or only) - FASTQ file path (default "fastq_1"). - second_col (str): The name of the column that contains the second (if any) - FASTQ file path (default "fastq_2"). - single_col (str): The name of the new column that will be inserted and - records whether the sample contains single- or paired-end sequencing - reads (default "single_end"). - - """ - super().__init__(**kwargs) - self._sample_col = sample_col - self._first_col = first_col - self._second_col = second_col - self._single_col = single_col - self._seen = set() - self.modified = [] - - def validate_and_transform(self, row): - """ - Perform all validations on the given row and insert the read pairing status. - - Args: - row (dict): A mapping from column headers (keys) to elements of that row - (values). - - """ - self._validate_sample(row) - self._validate_first(row) - self._validate_second(row) - self._validate_pair(row) - self._seen.add((row[self._sample_col], row[self._first_col])) - self.modified.append(row) - - def _validate_sample(self, row): - """Assert that the sample name exists and convert spaces to underscores.""" - if len(row[self._sample_col]) <= 0: - raise AssertionError("Sample input is required.") - # Sanitize samples slightly. - row[self._sample_col] = row[self._sample_col].replace(" ", "_") - - def _validate_first(self, row): - """Assert that the first FASTQ entry is non-empty and has the right format.""" - if len(row[self._first_col]) <= 0: - raise AssertionError("At least the first FASTQ file is required.") - self._validate_fastq_format(row[self._first_col]) - - def _validate_second(self, row): - """Assert that the second FASTQ entry has the right format if it exists.""" - if len(row[self._second_col]) > 0: - self._validate_fastq_format(row[self._second_col]) - - def _validate_pair(self, row): - """Assert that read pairs have the same file extension. Report pair status.""" - if row[self._first_col] and row[self._second_col]: - row[self._single_col] = False - first_col_suffix = Path(row[self._first_col]).suffixes[-2:] - second_col_suffix = Path(row[self._second_col]).suffixes[-2:] - if first_col_suffix != second_col_suffix: - raise AssertionError("FASTQ pairs must have the same file extensions.") - else: - row[self._single_col] = True - - def _validate_fastq_format(self, filename): - """Assert that a given filename has one of the expected FASTQ extensions.""" - if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): - raise AssertionError( - f"The FASTQ file has an unrecognized extension: {filename}\n" - f"It should be one of: {', '.join(self.VALID_FORMATS)}" - ) - - def validate_unique_samples(self): - """ - Assert that the combination of sample name and FASTQ filename is unique. - - In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. - - """ - if len(self._seen) != len(self.modified): - raise AssertionError("The pair of sample name and FASTQ must be unique.") - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - row[self._sample_col] = f"{sample}_T{seen[sample]}" - - -def read_head(handle, num_lines=10): - """Read the specified number of lines from the current position in the file.""" - lines = [] - for idx, line in enumerate(handle): - if idx == num_lines: - break - lines.append(line) - return "".join(lines) - - -def sniff_format(handle): - """ - Detect the tabular format. - - Args: - handle (text file): A handle to a `text file`_ object. The read position is - expected to be at the beginning (index 0). - - Returns: - csv.Dialect: The detected tabular format. - - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - dialect = sniffer.sniff(peek) - return dialect - - -def check_samplesheet(file_in, file_out): - """ - Check that the tabular samplesheet has the structure expected by nf-core pipelines. - - Validate the general shape of the table, expected columns, and each row. Also add - an additional column which records whether one or two FASTQ reads were found. - - Args: - file_in (pathlib.Path): The given tabular samplesheet. The format can be either - CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. - file_out (pathlib.Path): Where the validated and transformed samplesheet should - be created; always in CSV format. - - Example: - This function checks that the samplesheet follows the following structure, - see also the `viral recon samplesheet`_:: - - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, - - .. _viral recon samplesheet: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv - - """ - required_columns = {"sample", "fastq_1", "fastq_2"} - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_in.open(newline="") as in_handle: - reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) - # Validate the existence of the expected header columns. - if not required_columns.issubset(reader.fieldnames): - req_cols = ", ".join(required_columns) - logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") - sys.exit(1) - # Validate each row. - checker = RowChecker() - for i, row in enumerate(reader): - try: - checker.validate_and_transform(row) - except AssertionError as error: - logger.critical(f"{str(error)} On line {i + 2}.") - sys.exit(1) - checker.validate_unique_samples() - header = list(reader.fieldnames) - header.insert(1, "single_end") - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_out.open(mode="w", newline="") as out_handle: - writer = csv.DictWriter(out_handle, header, delimiter=",") - writer.writeheader() - for row in checker.modified: - writer.writerow(row) - - -def parse_args(argv=None): - """Define and immediately parse command line arguments.""" - parser = argparse.ArgumentParser( - description="Validate and transform a tabular samplesheet.", - epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", - ) - parser.add_argument( - "file_in", - metavar="FILE_IN", - type=Path, - help="Tabular input samplesheet in CSV or TSV format.", - ) - parser.add_argument( - "file_out", - metavar="FILE_OUT", - type=Path, - help="Transformed output samplesheet in CSV format.", - ) - parser.add_argument( - "-l", - "--log-level", - help="The desired log level (default WARNING).", - choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), - default="WARNING", - ) - return parser.parse_args(argv) - - -def main(argv=None): - """Coordinate argument parsing and program execution.""" - args = parse_args(argv) - logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") - if not args.file_in.is_file(): - logger.error(f"The given input file {args.file_in} was not found!") - sys.exit(2) - args.file_out.parent.mkdir(parents=True, exist_ok=True) - check_samplesheet(args.file_in, args.file_out) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/conf/modules.config b/conf/modules.config index d91c6aba..e3ea8fa6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,14 +18,6 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: SAMPLESHEET_CHECK { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: FASTQC { ext.args = '--quiet' } diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index e248e4c3..00000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,356 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Warn if a -profile or Nextflow config has not been provided to run the pipeline - // - public static void checkConfigProvided(workflow, log) { - if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " - } - } - - // - // Generate version string - // - public static String version(workflow) { - String version_string = "" - - if (workflow.manifest.version) { - def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' - version_string += "${prefix_v}${workflow.manifest.version}" - } - - if (workflow.commitId) { - def git_shortsha = workflow.commitId.substring(0, 7) - version_string += "-g${git_shortsha}" - } - - return version_string - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = NfcoreTemplate.version(workflow) - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" - } - mqc_report = mqc_report[0] - } - } - } catch (all) { - if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" - } - } - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") - sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); - output_hf.delete() - - // Write summary e-mail TXT to a file - def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); - output_tf.delete() - } - - // - // Construct and send a notification to a web server as JSON - // e.g. Microsoft Teams and Slack - // - public static void IM_notification(workflow, params, summary_params, projectDir, log) { - def hook_url = params.hook_url - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp - - def msg_fields = [:] - msg_fields['version'] = NfcoreTemplate.version(workflow) - msg_fields['runName'] = workflow.runName - msg_fields['success'] = workflow.success - msg_fields['dateComplete'] = workflow.complete - msg_fields['duration'] = workflow.duration - msg_fields['exitStatus'] = workflow.exitStatus - msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") - msg_fields['projectDir'] = workflow.projectDir - msg_fields['summary'] = summary << misc_fields - - // Render the JSON template - def engine = new groovy.text.GStringTemplateEngine() - // Different JSON depending on the service provider - // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format - def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" - def hf = new File("$projectDir/assets/${json_path}") - def json_template = engine.createTemplate(hf).make(msg_fields) - def json_message = json_template.toString() - - // POST - def post = new URL(hook_url).openConnection(); - post.setRequestMethod("POST") - post.setDoOutput(true) - post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); - } - } - - // - // Dump pipeline parameters in a json file - // - public static void dump_parameters(workflow, params) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) - - FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") - temp_pf.delete() - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // nf-core logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String workflow_version = NfcoreTemplate.version(workflow) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100644 index 8d030f4e..00000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] - def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean - - // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } - - if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - } - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index bdcfe027..00000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,77 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the nf-core/pangenome pipeline -// - -import nextflow.Nextflow - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log, args) { - - // Print workflow version and exit on --version - if (params.version) { - String workflow_version = NfcoreTemplate.version(workflow) - log.info "${workflow.manifest.name} ${workflow_version}" - System.exit(0) - } - - // Check that a -profile or Nextflow config has been provided to run the pipeline - NfcoreTemplate.checkConfigProvided(workflow, log) - // Check that the profile doesn't contain spaces and doesn't end with a trailing comma - checkProfile(workflow.profile, args, log) - - // Check that conda channels are set-up correctly - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - - // Check input has been provided - if (!params.input) { - Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") - } - } - // - // Get attribute from genome config file e.g. fasta - // - public static Object getGenomeAttribute(params, attribute) { - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - return params.genomes[ params.genome ][ attribute ] - } - } - return null - } - - // - // Exit pipeline if --profile contains spaces - // - private static void checkProfile(profile, args, log) { - if (profile.endsWith(',')) { - Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - if (args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - } -} diff --git a/lib/WorkflowPangenome.groovy b/lib/WorkflowPangenome.groovy deleted file mode 100755 index 09dee956..00000000 --- a/lib/WorkflowPangenome.groovy +++ /dev/null @@ -1,122 +0,0 @@ -// -// This file holds several functions specific to the workflow/pangenome.nf in the nf-core/pangenome pipeline -// - -import nextflow.Nextflow -import groovy.text.SimpleTemplateEngine - -class WorkflowPangenome { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - - genomeExistsError(params, log) - - - if (!params.fasta) { - Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - } - } - - // - // Get workflow summary for MultiQC - // - public static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

$group

\n" - summary_section += "
\n" - for (param in group_params.keySet()) { - summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" - } - summary_section += "
\n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } - - // - // Generate methods description for MultiQC - // - - public static String toolCitationText(params) { - - // TODO nf-core: Optionally add in-text citation tools to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def citation_text = [ - "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." - ].join(' ').trim() - - return citation_text - } - - public static String toolBibliographyText(params) { - - // TODO Optionally add bibliographic entries to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " - ].join(' ').trim() - - return reference_text - } - - public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { - // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file - def meta = [:] - meta.workflow = run_workflow.toMap() - meta["manifest_map"] = run_workflow.manifest.toMap() - - // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " - - // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - //meta["tool_bibliography"] = toolBibliographyText(params) - - - def methods_text = mqc_methods_yaml.text - - def engine = new SimpleTemplateEngine() - def description_html = engine.createTemplate(methods_text).make(meta) - - return description_html - } - - // - // Exit pipeline if incorrect --genome key provided - // - private static void genomeExistsError(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - Nextflow.error(error_string) - } - } -} diff --git a/main.nf b/main.nf index ee2f7f3a..b3ab1e36 100644 --- a/main.nf +++ b/main.nf @@ -13,66 +13,96 @@ nextflow.enable.dsl = 2 /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// TODO nf-core: Remove this line if you don't need a FASTA file -// This is an example of how to use getGenomeAttribute() to fetch parameters -// from igenomes.config using `--genome` -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +include { PANGENOME } from './workflows/pangenome' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_pangenome_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_pangenome_pipeline' + +include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_pangenome_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE & PRINT PARAMETER SUMMARY + GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { validateParameters; paramsHelp } from 'plugin/nf-validation' - -// Print help message if needed -if (params.help) { - def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) - def citation = '\n' + WorkflowMain.citation(workflow) + '\n' - def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" - log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs) - System.exit(0) -} - -// Validate input parameters -if (params.validate_params) { - validateParameters() -} - -WorkflowMain.initialise(workflow, params, log, args) +// TODO nf-core: Remove this line if you don't need a FASTA file +// This is an example of how to use getGenomeAttribute() to fetch parameters +// from igenomes.config using `--genome` +params.fasta = getGenomeAttribute('fasta') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - NAMED WORKFLOW FOR PIPELINE + NAMED WORKFLOWS FOR PIPELINE ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { PANGENOME } from './workflows/pangenome' - // -// WORKFLOW: Run main nf-core/pangenome analysis pipeline +// WORKFLOW: Run main analysis pipeline depending on type of input // workflow NFCORE_PANGENOME { - PANGENOME () -} + take: + samplesheet // channel: samplesheet read in from --input + + main: + + // + // WORKFLOW: Run pipeline + // + PANGENOME ( + samplesheet + ) + + emit: + multiqc_report = PANGENOME.out.multiqc_report // channel: /path/to/multiqc_report.html + +} /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - RUN ALL WORKFLOWS + RUN MAIN WORKFLOW ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// -// WORKFLOW: Execute a single named workflow for the pipeline -// See: https://github.com/nf-core/rnaseq/issues/619 -// workflow { - NFCORE_PANGENOME () + + main: + + // + // SUBWORKFLOW: Run initialisation tasks + // + PIPELINE_INITIALISATION ( + params.version, + params.help, + params.validate_params, + params.monochrome_logs, + args, + params.outdir, + params.input + ) + + // + // WORKFLOW: Run main workflow + // + NFCORE_PANGENOME ( + PIPELINE_INITIALISATION.out.samplesheet + ) + + // + // SUBWORKFLOW: Run completion tasks + // + PIPELINE_COMPLETION ( + params.email, + params.email_on_fail, + params.plaintext_email, + params.outdir, + params.monochrome_logs, + params.hook_url, + NFCORE_PANGENOME.out.multiqc_report + ) } /* diff --git a/modules.json b/modules.json index 1ff04d69..2914d0d4 100644 --- a/modules.json +++ b/modules.json @@ -5,22 +5,36 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - }, "fastqc": { "branch": "master", - "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", + "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", "installed_by": ["modules"] } } + }, + "subworkflows": { + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + } + } } } } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index d17dedff..00000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,31 +0,0 @@ -process SAMPLESHEET_CHECK { - tag "$samplesheet" - label 'process_single' - - conda "conda-forge::python=3.8.3" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" - - input: - path samplesheet - - output: - path '*.csv' , emit: csv - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: // This script is bundled with the pipeline, in nf-core/pangenome/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml deleted file mode 100644 index 9b3272bc..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: custom_dumpsoftwareversions -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf deleted file mode 100644 index f2187611..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ /dev/null @@ -1,24 +0,0 @@ -process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_single' - - // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" - - input: - path versions - - output: - path "software_versions.yml" , emit: yml - path "software_versions_mqc.yml", emit: mqc_yml - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - template 'dumpsoftwareversions.py' -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml deleted file mode 100644 index 5f15a5fd..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ /dev/null @@ -1,37 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: custom_dumpsoftwareversions -description: Custom module used to dump software versions within the nf-core pipeline template -keywords: - - custom - - dump - - version -tools: - - custom: - description: Custom module used to dump software versions within the nf-core pipeline template - homepage: https://github.com/nf-core/tools - documentation: https://github.com/nf-core/tools - licence: ["MIT"] -input: - - versions: - type: file - description: YML file containing software versions - pattern: "*.yml" -output: - - yml: - type: file - description: Standard YML file containing software versions - pattern: "software_versions.yml" - - mqc_yml: - type: file - description: MultiQC custom content YML file containing software versions - pattern: "software_versions_mqc.yml" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" -maintainers: - - "@drpatelh" - - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py deleted file mode 100755 index e55b8d43..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ /dev/null @@ -1,102 +0,0 @@ -#!/usr/bin/env python - - -"""Provide functions to merge multiple versions.yml files.""" - - -import platform -from textwrap import dedent - -import yaml - - -def _make_versions_html(versions): - """Generate a tabular HTML output of all versions for MultiQC.""" - html = [ - dedent( - """\\ - -
    Process Name \\", + " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    - - - - - - - - """ - ) - ] - for process, tmp_versions in sorted(versions.items()): - html.append("") - for i, (tool, version) in enumerate(sorted(tmp_versions.items())): - html.append( - dedent( - f"""\\ - - - - - - """ - ) - ) - html.append("") - html.append("
    Process Name Software Version
    {process if (i == 0) else ''}{tool}{version}
    ") - return "\\n".join(html) - - -def main(): - """Load all version files and generate merged output.""" - versions_this_module = {} - versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, - } - - with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - - # aggregate versions by the module name (derived from fully-qualified process name) - versions_by_module = {} - for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - - versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", - } - - versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), - } - - with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) - with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - - with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) - - -if __name__ == "__main__": - main() diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test deleted file mode 100644 index b1e1630b..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ /dev/null @@ -1,43 +0,0 @@ -nextflow_process { - - name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" - script "../main.nf" - process "CUSTOM_DUMPSOFTWAREVERSIONS" - tag "modules" - tag "modules_nfcore" - tag "custom" - tag "dumpsoftwareversions" - tag "custom/dumpsoftwareversions" - - test("Should run without failures") { - when { - process { - """ - def tool1_version = ''' - TOOL1: - tool1: 0.11.9 - '''.stripIndent() - - def tool2_version = ''' - TOOL2: - tool2: 1.9 - '''.stripIndent() - - input[0] = Channel.of(tool1_version, tool2_version).collectFile() - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.versions, - file(process.out.mqc_yml[0]).readLines()[0..10], - file(process.out.yml[0]).readLines()[0..7] - ).match() - } - ) - } - } -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap deleted file mode 100644 index 5f59a936..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ /dev/null @@ -1,33 +0,0 @@ -{ - "Should run without failures": { - "content": [ - [ - "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" - ], - [ - "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", - " \\n\\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n \\n \\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n\\n\\n \\n\\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\" - ], - [ - "CUSTOM_DUMPSOFTWAREVERSIONS:", - " python: 3.11.7", - " yaml: 5.4.1", - "TOOL1:", - " tool1: 0.11.9", - "TOOL2:", - " tool2: '1.9'", - "Workflow:" - ] - ], - "timestamp": "2024-01-09T23:01:18.710682" - } -} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml deleted file mode 100644 index 405aa24a..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -custom/dumpsoftwareversions: - - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 1f21c664..70edae4d 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -33,7 +33,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_single") } ) } } @@ -63,7 +63,7 @@ nextflow_process { { assert path(process.out.html[0][1][0]).text.contains("") }, { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } ) } } @@ -89,7 +89,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } ) } } @@ -115,7 +115,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } ) } } @@ -153,7 +153,7 @@ nextflow_process { { assert path(process.out.html[0][1][2]).text.contains("") }, { assert path(process.out.html[0][1][3]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } ) } } @@ -179,7 +179,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } ) } } @@ -204,7 +204,7 @@ nextflow_process { { assert process.success }, { assert snapshot(process.out.html.collect { file(it[1]).getName() } + process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match() } + process.out.versions ).match("fastqc_stub") } ) } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 5d624bb8..86f7c311 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,5 +1,17 @@ { - "sarscov2 single-end [fastq] - stub": { + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { "content": [ [ "test.html", @@ -7,14 +19,70 @@ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:40:57.254299" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" }, - "versions": { + "fastqc_versions_custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:36:50.033627" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 7625b752..2212096a 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.19 + - bioconda::multiqc=1.20 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1b9f7c43..354f4430 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : + 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index d0438eda..f1c4242e 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -3,6 +3,7 @@ nextflow_process { name "Test Process MULTIQC" script "../main.nf" process "MULTIQC" + tag "modules" tag "modules_nfcore" tag "multiqc" @@ -12,7 +13,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -25,7 +26,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_single") } ) } @@ -36,7 +37,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] @@ -49,7 +50,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_config") } ) } } @@ -61,7 +62,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -75,7 +76,7 @@ nextflow_process { { assert snapshot(process.out.report.collect { file(it).getName() } + process.out.data.collect { file(it).getName() } + process.out.plots.collect { file(it).getName() } + - process.out.versions ).match() } + process.out.versions ).match("multiqc_stub") } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index d37e7304..c204b488 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -1,21 +1,41 @@ { - "versions": { + "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:02:49.911994" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:28:51.744211298" }, - "sarscov2 single-end [fastqc] - stub": { + "multiqc_stub": { "content": [ [ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:03:14.524346" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:28.847433492" + }, + "multiqc_versions_config": { + "content": [ + [ + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:13.223621555" } } \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 1b18be51..96493a24 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,9 +16,7 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - - - // MultiQC options + fasta = null// MultiQC options multiqc_config = null multiqc_title = null multiqc_logo = null @@ -43,7 +41,6 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - // Max resource options // Defaults only, expecting to be overwritten diff --git a/nextflow_schema.json b/nextflow_schema.json index 46b71dae..94aa0bd3 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -16,6 +16,7 @@ "type": "string", "format": "file-path", "exists": true, + "schema": "assets/schema_input.json", "mimetype": "text/csv", "pattern": "^\\S+\\.csv$", "description": "Path to comma-separated file containing information about the samples in the experiment.", diff --git a/pyproject.toml b/pyproject.toml index 7d08e1c8..56110621 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -3,11 +3,13 @@ [tool.ruff] line-length = 120 target-version = "py38" -select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] cache-dir = "~/.cache/ruff" -[tool.ruff.isort] +[tool.ruff.lint] +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] + +[tool.ruff.lint.isort] known-first-party = ["nf_core"] -[tool.ruff.per-file-ignores] +[tool.ruff.lint.per-file-ignores] "__init__.py" = ["E402", "F401"] diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf deleted file mode 100644 index 0aecf87f..00000000 --- a/subworkflows/local/input_check.nf +++ /dev/null @@ -1,44 +0,0 @@ -// -// Check input samplesheet and get read channels -// - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' - -workflow INPUT_CHECK { - take: - samplesheet // file: /path/to/samplesheet.csv - - main: - SAMPLESHEET_CHECK ( samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } - - emit: - reads // channel: [ val(meta), [ reads ] ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return fastq_meta -} diff --git a/subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf b/subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf new file mode 100644 index 00000000..dc90ad90 --- /dev/null +++ b/subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf @@ -0,0 +1,247 @@ +// +// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { fromSamplesheet } from 'plugin/nf-validation' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' +include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' +include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' +include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' +include { imNotification } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' +include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE +======================================================================================== +*/ + +workflow PIPELINE_INITIALISATION { + + take: + version // boolean: Display version and exit + help // boolean: Display help text + validate_params // boolean: Boolean whether to validate parameters against the schema at runtime + monochrome_logs // boolean: Do not use coloured log outputs + nextflow_cli_args // array: List of positional nextflow CLI args + outdir // string: The output directory where the results will be saved + input // string: Path to input samplesheet + + main: + + ch_versions = Channel.empty() + + // + // Print version and exit if required and dump pipeline parameters to JSON file + // + UTILS_NEXTFLOW_PIPELINE ( + version, + true, + outdir, + workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 + ) + + // + // Validate parameters and generate parameter summary to stdout + // + pre_help_text = nfCoreLogo(monochrome_logs) + post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) + def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --outdir " + UTILS_NFVALIDATION_PLUGIN ( + help, + workflow_command, + pre_help_text, + post_help_text, + validate_params, + "nextflow_schema.json" + ) + + // + // Check config provided to the pipeline + // + UTILS_NFCORE_PIPELINE ( + nextflow_cli_args + ) + // + // Custom validation for pipeline parameters + // + validateInputParameters() + + // + // Create channel from input file provided through params.input + // + Channel + .fromSamplesheet("input") + .map { + meta, fastq_1, fastq_2 -> + if (!fastq_2) { + return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] + } else { + return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] + } + } + .groupTuple() + .map { + validateInputSamplesheet(it) + } + .map { + meta, fastqs -> + return [ meta, fastqs.flatten() ] + } + .set { ch_samplesheet } + + emit: + samplesheet = ch_samplesheet + versions = ch_versions +} + +/* +======================================================================================== + SUBWORKFLOW FOR PIPELINE COMPLETION +======================================================================================== +*/ + +workflow PIPELINE_COMPLETION { + + take: + email // string: email address + email_on_fail // string: email address sent on pipeline failure + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output + hook_url // string: hook URL for notifications + multiqc_report // string: Path to MultiQC report + + main: + + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + + // + // Completion email and summary + // + workflow.onComplete { + if (email || email_on_fail) { + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + } + + completionSummary(monochrome_logs) + + if (hook_url) { + imNotification(summary_params, hook_url) + } + } +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ +// +// Check and validate pipeline parameters +// +def validateInputParameters() { + genomeExistsError() +}// +// Validate channels from input samplesheet +// +def validateInputSamplesheet(input) { + def (metas, fastqs) = input[1..2] + + // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + if (!endedness_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") + } + + return [ metas[0], fastqs ] +} +// +// Get attribute from genome config file e.g. fasta +// +def getGenomeAttribute(attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null +} + +// +// Exit pipeline if incorrect --genome key provided +// +def genomeExistsError() { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } +}// +// Generate methods description for MultiQC +// +def toolCitationText() { + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text +} + +def toolBibliographyText() { + // TODO nf-core: Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text +} + +def methodsDescriptionText(mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = workflow.toMap() + meta["manifest_map"] = workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + // meta["tool_bibliography"] = toolBibliographyText() + + + def methods_text = mqc_methods_yaml.text + + def engine = new groovy.text.SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html.toString() +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf new file mode 100644 index 00000000..ac31f28f --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -0,0 +1,126 @@ +// +// Subworkflow with functionality that may be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NEXTFLOW_PIPELINE { + + take: + print_version // boolean: print version + dump_parameters // boolean: dump parameters + outdir // path: base directory used to publish pipeline results + check_conda_channels // boolean: check conda channels + + main: + + // + // Print workflow version and exit on --version + // + if (print_version) { + log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + System.exit(0) + } + + // + // Dump pipeline parameters to a JSON file + // + if (dump_parameters && outdir) { + dumpParametersToJSON(outdir) + } + + // + // When running with Conda, warn if channels have not been set-up appropriately + // + if (check_conda_channels) { + checkCondaChannels() + } + + emit: + dummy_emit = true +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Generate version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Dump pipeline parameters to a JSON file +// +def dumpParametersToJSON(outdir) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() +} + +// +// When running with -profile conda, warn if channels have not been set-up appropriately +// +def checkCondaChannels() { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml new file mode 100644 index 00000000..e5c3a0a8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml @@ -0,0 +1,38 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NEXTFLOW_PIPELINE" +description: Subworkflow with functionality that may be useful for any Nextflow pipeline +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - print_version: + type: boolean + description: | + Print the version of the pipeline and exit + - dump_parameters: + type: boolean + description: | + Dump the parameters of the pipeline to a JSON file + - output_directory: + type: directory + description: Path to output dir to write JSON file to. + pattern: "results/" + - check_conda_channel: + type: boolean + description: | + Check if the conda channel priority is correct. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" + - "@drpatelh" +maintainers: + - "@adamrtalbot" + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..8ed4310c --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -0,0 +1,54 @@ + +nextflow_function { + + name "Test Functions" + script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Test Function getWorkflowVersion") { + + function "getWorkflowVersion" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dumpParametersToJSON") { + + function "dumpParametersToJSON" + + when { + function { + """ + // define inputs of the function here. Example: + input[0] = "$outputDir" + """.stripIndent() + } + } + + then { + assertAll( + { assert function.success } + ) + } + } + + test("Test Function checkCondaChannels") { + + function "checkCondaChannels" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..db2030f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,12 @@ +{ + "Test Function getWorkflowVersion": { + "content": [ + "v9.9.9" + ], + "timestamp": "2024-01-19T11:32:36.031083" + }, + "Test Function checkCondaChannels": { + "content": null, + "timestamp": "2024-01-19T11:32:50.456" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..f7c54bc6 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,123 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NEXTFLOW_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + workflow "UTILS_NEXTFLOW_PIPELINE" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Should run no inputs") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = false + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should print version") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = true + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } + ) + } + } + + test("Should dump params") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = params.outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should not create params JSON if no output directory") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = null + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config new file mode 100644 index 00000000..53574ffe --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml new file mode 100644 index 00000000..f8476112 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nextflow_pipeline: + - subworkflows/nf-core/utils_nextflow_pipeline/** diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf new file mode 100644 index 00000000..a8b55d6f --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -0,0 +1,440 @@ +// +// Subworkflow with utility functions specific to the nf-core pipeline template +// + +import org.yaml.snakeyaml.Yaml +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFCORE_PIPELINE { + + take: + nextflow_cli_args + + main: + valid_config = checkConfigProvided() + checkProfileProvided(nextflow_cli_args) + + emit: + valid_config +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Warn if a -profile or Nextflow config has not been provided to run the pipeline +// +def checkConfigProvided() { + valid_config = true + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + valid_config = false + } + return valid_config +} + +// +// Exit pipeline if --profile contains spaces +// +def checkProfileProvided(nextflow_cli_args) { + if (workflow.profile.endsWith(',')) { + error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } + if (nextflow_cli_args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } +} + +// +// Citation string for pipeline +// +def workflowCitation() { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " ${workflow.manifest.doi}\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" +} + +// +// Generate workflow version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Get software versions for pipeline +// +def processVersionsFromYAML(yaml_file) { + Yaml yaml = new Yaml() + versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + return yaml.dumpAsMap(versions).trim() +} + +// +// Get workflow version for pipeline +// +def workflowVersionToYAML() { + return """ + Workflow: + $workflow.manifest.name: ${getWorkflowVersion()} + Nextflow: $workflow.nextflow.version + """.stripIndent().trim() +} + +// +// Get channel of software versions used in pipeline in YAML format +// +def softwareVersionsToYAML(ch_versions) { + return ch_versions + .unique() + .map { processVersionsFromYAML(it) } + .unique() + .mix(Channel.of(workflowVersionToYAML())) +} + +// +// Get workflow summary for MultiQC +// +def paramsSummaryMultiqc(summary_params) { + def summary_section = '' + for (group in summary_params.keySet()) { + def group_params = summary_params.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + + return yaml_file_text +} + +// +// nf-core logo +// +def nfCoreLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + +// +// Return dashed line +// +def dashedLine(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" +} + +// +// ANSII colours used for terminal logging +// +def logColours(monochrome_logs=true) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes +} + +// +// Attach the multiqc report to email +// +def attachMultiqcReport(multiqc_report) { + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + return mqc_report +} + +// +// Construct and send completion email +// +def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = getWorkflowVersion() + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = attachMultiqcReport(multiqc_report) + + // Check if we are only sending emails on failure + def email_address = email + if (!email && email_on_fail && !workflow.success) { + email_address = email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("${workflow.projectDir}/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("${workflow.projectDir}/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(monochrome_logs) + if (email_address) { + try { + if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() +} + +// +// Print pipeline summary on completion +// +def completionSummary(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } +} + +// +// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack +// +def imNotification(summary_params, hook_url) { + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = getWorkflowVersion() + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("${workflow.projectDir}/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml new file mode 100644 index 00000000..d08d2434 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml @@ -0,0 +1,24 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFCORE_PIPELINE" +description: Subworkflow with utility functions specific to the nf-core pipeline template +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - nextflow_cli_args: + type: list + description: | + Nextflow CLI positional arguments +output: + - success: + type: boolean + description: | + Dummy output to indicate success +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..1dc317f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -0,0 +1,134 @@ + +nextflow_function { + + name "Test Functions" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Test Function checkConfigProvided") { + + function "checkConfigProvided" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function checkProfileProvided") { + + function "checkProfileProvided" + + when { + function { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function workflowCitation") { + + function "workflowCitation" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function nfCoreLogo") { + + function "nfCoreLogo" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dashedLine") { + + function "dashedLine" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function without logColours") { + + function "logColours" + + when { + function { + """ + input[0] = true + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function with logColours") { + function "logColours" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..10f948e6 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,138 @@ +{ + "Test Function checkProfileProvided": { + "content": null, + "timestamp": "2024-02-09T15:43:55.145717" + }, + "Test Function checkConfigProvided": { + "content": [ + true + ], + "timestamp": "2024-01-19T11:34:13.548431224" + }, + "Test Function nfCoreLogo": { + "content": [ + "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" + ], + "timestamp": "2024-01-19T11:34:38.840454873" + }, + "Test Function workflowCitation": { + "content": [ + "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" + ], + "timestamp": "2024-01-19T11:34:22.24352016" + }, + "Test Function without logColours": { + "content": [ + { + "reset": "", + "bold": "", + "dim": "", + "underlined": "", + "blink": "", + "reverse": "", + "hidden": "", + "black": "", + "red": "", + "green": "", + "yellow": "", + "blue": "", + "purple": "", + "cyan": "", + "white": "", + "bblack": "", + "bred": "", + "bgreen": "", + "byellow": "", + "bblue": "", + "bpurple": "", + "bcyan": "", + "bwhite": "", + "ublack": "", + "ured": "", + "ugreen": "", + "uyellow": "", + "ublue": "", + "upurple": "", + "ucyan": "", + "uwhite": "", + "iblack": "", + "ired": "", + "igreen": "", + "iyellow": "", + "iblue": "", + "ipurple": "", + "icyan": "", + "iwhite": "", + "biblack": "", + "bired": "", + "bigreen": "", + "biyellow": "", + "biblue": "", + "bipurple": "", + "bicyan": "", + "biwhite": "" + } + ], + "timestamp": "2024-01-19T11:35:04.418416984" + }, + "Test Function dashedLine": { + "content": [ + "-\u001b[2m----------------------------------------------------\u001b[0m-" + ], + "timestamp": "2024-01-19T11:34:55.420000755" + }, + "Test Function with logColours": { + "content": [ + { + "reset": "\u001b[0m", + "bold": "\u001b[1m", + "dim": "\u001b[2m", + "underlined": "\u001b[4m", + "blink": "\u001b[5m", + "reverse": "\u001b[7m", + "hidden": "\u001b[8m", + "black": "\u001b[0;30m", + "red": "\u001b[0;31m", + "green": "\u001b[0;32m", + "yellow": "\u001b[0;33m", + "blue": "\u001b[0;34m", + "purple": "\u001b[0;35m", + "cyan": "\u001b[0;36m", + "white": "\u001b[0;37m", + "bblack": "\u001b[1;30m", + "bred": "\u001b[1;31m", + "bgreen": "\u001b[1;32m", + "byellow": "\u001b[1;33m", + "bblue": "\u001b[1;34m", + "bpurple": "\u001b[1;35m", + "bcyan": "\u001b[1;36m", + "bwhite": "\u001b[1;37m", + "ublack": "\u001b[4;30m", + "ured": "\u001b[4;31m", + "ugreen": "\u001b[4;32m", + "uyellow": "\u001b[4;33m", + "ublue": "\u001b[4;34m", + "upurple": "\u001b[4;35m", + "ucyan": "\u001b[4;36m", + "uwhite": "\u001b[4;37m", + "iblack": "\u001b[0;90m", + "ired": "\u001b[0;91m", + "igreen": "\u001b[0;92m", + "iyellow": "\u001b[0;93m", + "iblue": "\u001b[0;94m", + "ipurple": "\u001b[0;95m", + "icyan": "\u001b[0;96m", + "iwhite": "\u001b[0;97m", + "biblack": "\u001b[1;90m", + "bired": "\u001b[1;91m", + "bigreen": "\u001b[1;92m", + "biyellow": "\u001b[1;93m", + "biblue": "\u001b[1;94m", + "bipurple": "\u001b[1;95m", + "bicyan": "\u001b[1;96m", + "biwhite": "\u001b[1;97m" + } + ], + "timestamp": "2024-01-19T11:35:13.436366565" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..8940d32d --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,29 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFCORE_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + workflow "UTILS_NFCORE_PIPELINE" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Should run without failures") { + + when { + workflow { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap new file mode 100644 index 00000000..d07ce54c --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -0,0 +1,15 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + true + ], + "valid_config": [ + true + ] + } + ], + "timestamp": "2024-01-19T11:35:22.538940073" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config new file mode 100644 index 00000000..d0a926bf --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml new file mode 100644 index 00000000..ac8523c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfcore_pipeline: + - subworkflows/nf-core/utils_nfcore_pipeline/** diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf new file mode 100644 index 00000000..2585b65d --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf @@ -0,0 +1,62 @@ +// +// Subworkflow that uses the nf-validation plugin to render help text and parameter summary +// + +/* +======================================================================================== + IMPORT NF-VALIDATION PLUGIN +======================================================================================== +*/ + +include { paramsHelp } from 'plugin/nf-validation' +include { paramsSummaryLog } from 'plugin/nf-validation' +include { validateParameters } from 'plugin/nf-validation' + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFVALIDATION_PLUGIN { + + take: + print_help // boolean: print help + workflow_command // string: default commmand used to run pipeline + pre_help_text // string: string to be printed before help text and summary log + post_help_text // string: string to be printed after help text and summary log + validate_params // boolean: validate parameters + schema_filename // path: JSON schema file, null to use default value + + main: + + log.debug "Using schema file: ${schema_filename}" + + // Default values for strings + pre_help_text = pre_help_text ?: '' + post_help_text = post_help_text ?: '' + workflow_command = workflow_command ?: '' + + // + // Print help message if needed + // + if (print_help) { + log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text + System.exit(0) + } + + // + // Print parameter summary to stdout + // + log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text + + // + // Validate parameters relative to the parameter JSON schema + // + if (validate_params){ + validateParameters(parameters_schema: schema_filename) + } + + emit: + dummy_emit = true +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml new file mode 100644 index 00000000..3d4a6b04 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFVALIDATION_PLUGIN" +description: Use nf-validation to initiate and validate a pipeline +keywords: + - utility + - pipeline + - initialise + - validation +components: [] +input: + - print_help: + type: boolean + description: | + Print help message and exit + - workflow_command: + type: string + description: | + The command to run the workflow e.g. "nextflow run main.nf" + - pre_help_text: + type: string + description: | + Text to print before the help message + - post_help_text: + type: string + description: | + Text to print after the help message + - validate_params: + type: boolean + description: | + Validate the parameters and error if invalid. + - schema_filename: + type: string + description: | + The filename of the schema to validate against. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test new file mode 100644 index 00000000..517ee54e --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -0,0 +1,200 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFVALIDATION_PLUGIN" + script "../main.nf" + workflow "UTILS_NFVALIDATION_PLUGIN" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "plugin/nf-validation" + tag "'plugin/nf-validation'" + tag "utils_nfvalidation_plugin" + tag "subworkflows/utils_nfvalidation_plugin" + + test("Should run nothing") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should run help") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with command") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with extra text") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = "pre-help-text" + post_help_text = "post-help-text" + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('pre-help-text') } }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } }, + { assert workflow.stdout.any { it.contains('post-help-text') } } + ) + } + } + + test("Should validate params") { + + when { + + params { + monochrome_logs = true + test_data = '' + outdir = 1 + } + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = true + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json new file mode 100644 index 00000000..7626c1c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json @@ -0,0 +1,96 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", + "title": ". pipeline parameters", + "description": "", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["outdir"], + "properties": { + "validate_params": { + "type": "boolean", + "description": "Validate parameters?", + "default": true, + "hidden": true + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules", + "description": "Base for test data directory", + "hidden": true + }, + "test_data": { + "type": "string", + "description": "Fake test data param", + "hidden": true + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "logo": { + "type": "boolean", + "default": true, + "description": "Display nf-core logo in console output.", + "fa_icon": "fas fa-image", + "hidden": true + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Pull Singularity container from Docker?", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Use monochrome_logs", + "hidden": true + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml new file mode 100644 index 00000000..60b1cfff --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfvalidation_plugin: + - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/workflows/pangenome.nf b/workflows/pangenome.nf index 41ceb16e..40da5b37 100644 --- a/workflows/pangenome.nf +++ b/workflows/pangenome.nf @@ -1,54 +1,15 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - PRINT PARAMS SUMMARY + IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' - -def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) -def citation = '\n' + WorkflowMain.citation(workflow) + '\n' -def summary_params = paramsSummaryMap(workflow) - -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - -WorkflowPangenome.initialise(params, log) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT LOCAL MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -include { INPUT_CHECK } from '../subworkflows/local/input_check' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT NF-CORE MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// MODULE: Installed directly from nf-core/modules -// -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_pangenome_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -56,50 +17,45 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// Info required for completion email and summary -def multiqc_report = [] - workflow PANGENOME { - ch_versions = Channel.empty() + take: + ch_samplesheet // channel: samplesheet read in from --input - // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files - // - INPUT_CHECK ( - file(params.input) - ) - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema + main: + + ch_versions = Channel.empty() + ch_multiqc_files = Channel.empty() // // MODULE: Run FastQC // FASTQC ( - INPUT_CHECK.out.reads + ch_samplesheet ) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique().collectFile(name: 'collated_versions.yml') - ) + // + // Collate and save software versions + // + softwareVersionsToYAML(ch_versions) + .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) + .set { ch_collated_versions } // // MODULE: MultiQC // - workflow_summary = WorkflowPangenome.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowPangenome.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) MULTIQC ( ch_multiqc_files.collect(), @@ -107,31 +63,10 @@ workflow PANGENOME { ch_multiqc_custom_config.toList(), ch_multiqc_logo.toList() ) - multiqc_report = MULTIQC.out.report.toList() -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.dump_parameters(workflow, params) - NfcoreTemplate.summary(workflow, params, log) - if (params.hook_url) { - NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) - } -} -workflow.onError { - if (workflow.errorReport.contains("Process requirement exceeds available memory")) { - println("🛑 Default resources exceed availability 🛑 ") - println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") - } + emit: + multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [ path(versions.yml) ] } /* From 49a9fd716cccf31b766aacb20d113ccbe44ae5d1 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Fri, 23 Feb 2024 11:33:08 +0100 Subject: [PATCH 53/83] update schema for better clarity --- nextflow_schema.json | 16 ++++++++-------- 1 file changed, 8 insertions(+), 8 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 95e9cd2b..533d940b 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -54,13 +54,13 @@ "properties": { "wfmash_map_pct_id": { "type": "number", - "default": 90, + "default": 90.0, "description": "Percent identity in the wfmash mashmap step.", "help_text": "Use `mash dist` or `mash triangle` to explore the typical level of divergence between the sequences in your input (see https://pggb.readthedocs.io/en/latest/rst/tutorials/divergence_estimation.html#divergence-estimation for more information). Convert this to an approximate percent identity and provide it as --wfmash_map_pct_id . A list of examples can be found at https://github.com/pangenome/pggb#example-builds-for-diverse-species." }, "wfmash_segment_length": { - "type": "string", - "default": "5000", + "type": "integer", + "default": 5000, "description": "Segment length for mapping.", "help_text": "Crucially, --wfmash_segment_length provides a kind of minimum alignment length filter. The `mashmap3` step in `wfmash` will only consider segments of this size. For small pangenome graphs, or where there are few repeats, --wfmash_segment_length can be set low (for example 500 when building a MHC pangenome graph). However, for larger contexts, with repeats, it can be very important to set this high (for instance 50k in the case of human genomes). A long segment length ensures that we represent long collinear regions of the input sequences in the structure of the graph. In general, this should at least be larger than transposons and other common repeats in your pangenome. A list of examples can be found at https://github.com/pangenome/pggb#example-builds-for-diverse-species.", "pattern": "^([1-9]\\d*[kKmMgGtT]?|0)$" @@ -98,8 +98,7 @@ }, "wfmash_exclude_delim": { "type": "string", - "description": "Skip mappings between sequences with the same name prefix before the given delimiter character. [default: all-vs-all and !self].", - "hidden": true + "description": "Skip mappings between sequences with the same name prefix before the given delimiter character. [default: all-vs-all and !self]." }, "wfmash_temp_dir": { "type": "string", @@ -131,8 +130,8 @@ "help_text": "Graph induction with seqwish often works better when we filter very short matches out of the input alignments. In practice, these often occur in regions of low alignment quality, which are typical of areas with large INDELs and structural variations in the wfmash alignments. This underalignment is then resolved in the smoothxg step. Removing short matches can simplify the graph and remove spurious relationships caused by short repeated homologies.\nA setting of --seqwish_min_match_length 47 is optimal for around 5% divergence, and we suggest lowering it for higher divergence and increasing it for lower divergence. Values up to --seqwish_min_match_length 311 work well for human haplotypes. In effect, setting --seqwish_min_match_length to N means that we can tolerate a local pairwise difference rate of no more than 1/N. Thus, INDELs which may be represented by complex series of edit operations will be opened into bubbles in the induced graph, and alignment regions with very low identity will be ignored. Using affine-gapped alignment (such as with minimap2) may reduce the impact of this step by representing large indels more precisely in the input alignments. However, it remains important due to local inconsistency in alignments in low-complexity sequence." }, "seqwish_transclose_batch": { - "type": "string", - "default": "10000000", + "type": "integer", + "default": 10000000, "description": "Number of base pairs to use for transitive closure batch.", "help_text": "If you run out of memory during the seqwish step, you can lower this value. It will take longer, but it will use less memory.", "pattern": "^([1-9]\\d*[kKmMgGtT]?|0)$" @@ -459,7 +458,8 @@ "fa_icon": "far fa-check-circle", "description": "Validation of parameters in lenient more.", "hidden": true, - "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." + "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode).", + "default": true }, "show_hidden_params": { "type": "boolean", From bc43903c4ad59980fe5ed2a1ea2a4dfea080306b Mon Sep 17 00:00:00 2001 From: subwaystation Date: Fri, 23 Feb 2024 11:35:48 +0100 Subject: [PATCH 54/83] a bit stronger seqwish match filterings --- nextflow.config | 2 +- nextflow_schema.json | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow.config b/nextflow.config index 8c034fd2..10c4d044 100644 --- a/nextflow.config +++ b/nextflow.config @@ -29,7 +29,7 @@ params { // Seqwish options seqwish_paf = null - seqwish_min_match_length = 19 + seqwish_min_match_length = 23 seqwish_transclose_batch = 10000000 seqwish_sparse_factor = 0.0 seqwish_temp_dir = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 533d940b..2dc58003 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -125,7 +125,7 @@ "properties": { "seqwish_min_match_length": { "type": "integer", - "default": 19, + "default": 23, "description": "Ignores exact matches below this length.", "help_text": "Graph induction with seqwish often works better when we filter very short matches out of the input alignments. In practice, these often occur in regions of low alignment quality, which are typical of areas with large INDELs and structural variations in the wfmash alignments. This underalignment is then resolved in the smoothxg step. Removing short matches can simplify the graph and remove spurious relationships caused by short repeated homologies.\nA setting of --seqwish_min_match_length 47 is optimal for around 5% divergence, and we suggest lowering it for higher divergence and increasing it for lower divergence. Values up to --seqwish_min_match_length 311 work well for human haplotypes. In effect, setting --seqwish_min_match_length to N means that we can tolerate a local pairwise difference rate of no more than 1/N. Thus, INDELs which may be represented by complex series of edit operations will be opened into bubbles in the induced graph, and alignment regions with very low identity will be ignored. Using affine-gapped alignment (such as with minimap2) may reduce the impact of this step by representing large indels more precisely in the input alignments. However, it remains important due to local inconsistency in alignments in low-complexity sequence." }, From 6d248e30c5c352fb20492e4940bdb694324c2b97 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Fri, 23 Feb 2024 11:41:36 +0100 Subject: [PATCH 55/83] it is complicated... --- nextflow_schema.json | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 2dc58003..87a60ffd 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -59,7 +59,7 @@ "help_text": "Use `mash dist` or `mash triangle` to explore the typical level of divergence between the sequences in your input (see https://pggb.readthedocs.io/en/latest/rst/tutorials/divergence_estimation.html#divergence-estimation for more information). Convert this to an approximate percent identity and provide it as --wfmash_map_pct_id . A list of examples can be found at https://github.com/pangenome/pggb#example-builds-for-diverse-species." }, "wfmash_segment_length": { - "type": "integer", + "type": "string", "default": 5000, "description": "Segment length for mapping.", "help_text": "Crucially, --wfmash_segment_length provides a kind of minimum alignment length filter. The `mashmap3` step in `wfmash` will only consider segments of this size. For small pangenome graphs, or where there are few repeats, --wfmash_segment_length can be set low (for example 500 when building a MHC pangenome graph). However, for larger contexts, with repeats, it can be very important to set this high (for instance 50k in the case of human genomes). A long segment length ensures that we represent long collinear regions of the input sequences in the structure of the graph. In general, this should at least be larger than transposons and other common repeats in your pangenome. A list of examples can be found at https://github.com/pangenome/pggb#example-builds-for-diverse-species.", @@ -130,7 +130,7 @@ "help_text": "Graph induction with seqwish often works better when we filter very short matches out of the input alignments. In practice, these often occur in regions of low alignment quality, which are typical of areas with large INDELs and structural variations in the wfmash alignments. This underalignment is then resolved in the smoothxg step. Removing short matches can simplify the graph and remove spurious relationships caused by short repeated homologies.\nA setting of --seqwish_min_match_length 47 is optimal for around 5% divergence, and we suggest lowering it for higher divergence and increasing it for lower divergence. Values up to --seqwish_min_match_length 311 work well for human haplotypes. In effect, setting --seqwish_min_match_length to N means that we can tolerate a local pairwise difference rate of no more than 1/N. Thus, INDELs which may be represented by complex series of edit operations will be opened into bubbles in the induced graph, and alignment regions with very low identity will be ignored. Using affine-gapped alignment (such as with minimap2) may reduce the impact of this step by representing large indels more precisely in the input alignments. However, it remains important due to local inconsistency in alignments in low-complexity sequence." }, "seqwish_transclose_batch": { - "type": "integer", + "type": "string", "default": 10000000, "description": "Number of base pairs to use for transitive closure batch.", "help_text": "If you run out of memory during the seqwish step, you can lower this value. It will take longer, but it will use less memory.", From cf5393c1c9fef755934c78d6b3f2565a5de0eceb Mon Sep 17 00:00:00 2001 From: subwaystation Date: Fri, 23 Feb 2024 11:52:12 +0100 Subject: [PATCH 56/83] new param wfmash_hg_filter_ani_diff --- conf/modules.config | 4 ++++ nextflow.config | 1 + nextflow_schema.json | 12 ++++++++---- 3 files changed, 13 insertions(+), 4 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index f9bd2857..a69540b5 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -113,6 +113,7 @@ process { "-H ${params.wfmash_mash_kmer_thres}", "${wfmash_sparse_map_cmd}", params.wfmash_temp_dir ? "-B ${wfmash_temp_dir}" : "", + "-2 ${params.wfmash_hg_filter_ani_diff}", ].join(" ").trim() } publishDir = [ @@ -137,6 +138,7 @@ process { "${wfmash_sparse_map_cmd}", params.wfmash_temp_dir ? "-B ${wfmash_temp_dir}" : "", "-m", + "-2 ${params.wfmash_hg_filter_ani_diff}", ].join(" ").trim() } publishDir = [ @@ -161,6 +163,7 @@ process { "${wfmash_sparse_map_cmd}", params.wfmash_temp_dir ? "-B ${wfmash_temp_dir}" : "", "-m", + "-2 ${params.wfmash_hg_filter_ani_diff}", ].join(" ").trim() } publishDir = [ @@ -194,6 +197,7 @@ process { "${wfmash_sparse_map_cmd}", params.wfmash_temp_dir ? "-B ${wfmash_temp_dir}" : "", "--invert-filtering", + "-2 ${params.wfmash_hg_filter_ani_diff}", ].join(" ").trim() } publishDir = [ diff --git a/nextflow.config b/nextflow.config index 10c4d044..9b9b42c3 100644 --- a/nextflow.config +++ b/nextflow.config @@ -26,6 +26,7 @@ params { wfmash_chunks = 1 wfmash_only = false wfmash_temp_dir = null + wfmash_hg_filter_ani_diff = 30 // Seqwish options seqwish_paf = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 87a60ffd..56eb9926 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -54,13 +54,13 @@ "properties": { "wfmash_map_pct_id": { "type": "number", - "default": 90.0, + "default": 90, "description": "Percent identity in the wfmash mashmap step.", "help_text": "Use `mash dist` or `mash triangle` to explore the typical level of divergence between the sequences in your input (see https://pggb.readthedocs.io/en/latest/rst/tutorials/divergence_estimation.html#divergence-estimation for more information). Convert this to an approximate percent identity and provide it as --wfmash_map_pct_id . A list of examples can be found at https://github.com/pangenome/pggb#example-builds-for-diverse-species." }, "wfmash_segment_length": { "type": "string", - "default": 5000, + "default": "5000", "description": "Segment length for mapping.", "help_text": "Crucially, --wfmash_segment_length provides a kind of minimum alignment length filter. The `mashmap3` step in `wfmash` will only consider segments of this size. For small pangenome graphs, or where there are few repeats, --wfmash_segment_length can be set low (for example 500 when building a MHC pangenome graph). However, for larger contexts, with repeats, it can be very important to set this high (for instance 50k in the case of human genomes). A long segment length ensures that we represent long collinear regions of the input sequences in the structure of the graph. In general, this should at least be larger than transposons and other common repeats in your pangenome. A list of examples can be found at https://github.com/pangenome/pggb#example-builds-for-diverse-species.", "pattern": "^([1-9]\\d*[kKmMgGtT]?|0)$" @@ -114,6 +114,10 @@ "wfmash_only": { "type": "boolean", "description": "If this parameter is set, only the wfmash alignment step of the pipeline is executed. This option is offered for users who want to run wfmash on a cluster." + }, + "wfmash_hg_filter_ani_diff": { + "type": "integer", + "default": 30 } } }, @@ -131,14 +135,14 @@ }, "seqwish_transclose_batch": { "type": "string", - "default": 10000000, + "default": "10000000", "description": "Number of base pairs to use for transitive closure batch.", "help_text": "If you run out of memory during the seqwish step, you can lower this value. It will take longer, but it will use less memory.", "pattern": "^([1-9]\\d*[kKmMgGtT]?|0)$" }, "seqwish_sparse_factor": { "type": "number", - "default": 0.0, + "default": 0, "description": "Keep this randomly selected fraction of input matches." }, "seqwish_temp_dir": { From b63c73cec9bf7a3d367507ea3018fb949d425e7d Mon Sep 17 00:00:00 2001 From: subwaystation Date: Fri, 23 Feb 2024 12:04:15 +0100 Subject: [PATCH 57/83] update vg deconstruct --- modules/local/vg_deconstruct/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/vg_deconstruct/main.nf b/modules/local/vg_deconstruct/main.nf index ece466eb..830723f5 100644 --- a/modules/local/vg_deconstruct/main.nf +++ b/modules/local/vg_deconstruct/main.nf @@ -65,7 +65,7 @@ process VG_DECONSTRUCT { #TODO: to remove when vcfwave will be bug-free # The TYPE info sometimes is wrong/missing # There are variants without the ALT allele - bcftools annotate -x INFO/TYPE \$vcf_decomposed_tmp | awk '\$5 != "."' > \$vcf_decomposed + bcftools sort -T "$temp_dir" "$vcf_decomposed_tmp" | bcftools annotate -x INFO/TYPE \$vcf_decomposed_tmp | awk '\$5 != "."' > \$vcf_decomposed rm \$vcf_decomposed_tmp \$vcf.gz bcftools stats \$vcf_decomposed > \$vcf_decomposed.stats fi From 4f4ae873aef6ea55002f3c4003ac8b782d841083 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Fri, 23 Feb 2024 13:58:56 +0100 Subject: [PATCH 58/83] fix vg deconstruct2 --- modules/local/vg_deconstruct/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/vg_deconstruct/main.nf b/modules/local/vg_deconstruct/main.nf index 830723f5..d2d65ee3 100644 --- a/modules/local/vg_deconstruct/main.nf +++ b/modules/local/vg_deconstruct/main.nf @@ -65,7 +65,7 @@ process VG_DECONSTRUCT { #TODO: to remove when vcfwave will be bug-free # The TYPE info sometimes is wrong/missing # There are variants without the ALT allele - bcftools sort -T "$temp_dir" "$vcf_decomposed_tmp" | bcftools annotate -x INFO/TYPE \$vcf_decomposed_tmp | awk '\$5 != "."' > \$vcf_decomposed + bcftools sort "$vcf_decomposed_tmp" | bcftools annotate -x INFO/TYPE \$vcf_decomposed_tmp | awk '\$5 != "."' > \$vcf_decomposed rm \$vcf_decomposed_tmp \$vcf.gz bcftools stats \$vcf_decomposed > \$vcf_decomposed.stats fi From d4168fb8528c8ff4ef171dfd07e1e4f7c7372f44 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Fri, 23 Feb 2024 14:02:10 +0100 Subject: [PATCH 59/83] fix vg deconstruct2 --- modules/local/vg_deconstruct/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/vg_deconstruct/main.nf b/modules/local/vg_deconstruct/main.nf index d2d65ee3..ae6092f0 100644 --- a/modules/local/vg_deconstruct/main.nf +++ b/modules/local/vg_deconstruct/main.nf @@ -65,7 +65,7 @@ process VG_DECONSTRUCT { #TODO: to remove when vcfwave will be bug-free # The TYPE info sometimes is wrong/missing # There are variants without the ALT allele - bcftools sort "$vcf_decomposed_tmp" | bcftools annotate -x INFO/TYPE \$vcf_decomposed_tmp | awk '\$5 != "."' > \$vcf_decomposed + bcftools sort \$vcf_decomposed_tmp | bcftools annotate -x INFO/TYPE \$vcf_decomposed_tmp | awk '\$5 != "."' > \$vcf_decomposed rm \$vcf_decomposed_tmp \$vcf.gz bcftools stats \$vcf_decomposed > \$vcf_decomposed.stats fi From a881e60b1f6b0988dcc9234c5820986bad2dcf07 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 26 Feb 2024 14:57:54 +0100 Subject: [PATCH 60/83] 1st step of the blog post --- main.nf | 79 +-- modules.json | 18 +- .../utils_nfcore_pangenome_pipeline/main.nf | 472 ++++++++++++++++++ .../nf-core/utils_nextflow_pipeline/main.nf | 126 +++++ .../nf-core/utils_nextflow_pipeline/meta.yml | 38 ++ .../tests/main.function.nf.test | 54 ++ .../tests/main.function.nf.test.snap | 12 + .../tests/main.workflow.nf.test | 123 +++++ .../tests/nextflow.config | 9 + .../utils_nextflow_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfcore_pipeline/main.nf | 440 ++++++++++++++++ .../nf-core/utils_nfcore_pipeline/meta.yml | 24 + .../tests/main.function.nf.test | 134 +++++ .../tests/main.function.nf.test.snap | 138 +++++ .../tests/main.workflow.nf.test | 29 ++ .../tests/main.workflow.nf.test.snap | 15 + .../tests/nextflow.config | 9 + .../utils_nfcore_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfvalidation_plugin/main.nf | 62 +++ .../utils_nfvalidation_plugin/meta.yml | 44 ++ .../tests/main.nf.test | 200 ++++++++ .../tests/nextflow_schema.json | 96 ++++ .../utils_nfvalidation_plugin/tests/tags.yml | 2 + workflows/pangenome.nf | 91 +--- 24 files changed, 2113 insertions(+), 106 deletions(-) create mode 100644 subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/main.nf b/main.nf index da551fad..474ac7d8 100644 --- a/main.nf +++ b/main.nf @@ -13,50 +13,30 @@ nextflow.enable.dsl = 2 /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// We dont't need this parameter in this pipeline -// params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE & PRINT PARAMETER SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -include { validateParameters; paramsHelp } from 'plugin/nf-validation' - -// Print help message if needed -if (params.help) { - def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) - def citation = '\n' + WorkflowMain.citation(workflow) + '\n' - def String command = "nextflow run ${workflow.manifest.name} --input --n_haplotypes --outdir -profile docker" - log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs) - System.exit(0) -} - -// Validate input parameters -if (params.validate_params) { - validateParameters() -} - -WorkflowMain.initialise(workflow, params, log, args) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - NAMED WORKFLOW FOR PIPELINE -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -include { PANGENOME } from './workflows/pangenome' +include { PANGENOME } from './workflows/pangenome' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_pangenome_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_pangenome_pipeline' // // WORKFLOW: Run main nf-core/pangenome analysis pipeline // workflow NFCORE_PANGENOME { + + main: + + ch_versions = Channel.empty() + PANGENOME () + + ch_versions = ch_versions.mix(PANGENOME.out.versions) + + emit: + multiqc_report = PANGENOME.out.multiqc_report // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [version1, version2, ...] } /* @@ -70,7 +50,36 @@ workflow NFCORE_PANGENOME { // See: https://github.com/nf-core/rnaseq/issues/619 // workflow { + + main: + + + // + // SUBWORKFLOW: Run initialisation tasks + // + PIPELINE_INITIALISATION ( + params.version, + params.help, + params.validate_params, + params.monochrome_logs, + args, + params.outdir + ) + NFCORE_PANGENOME () + + // + // SUBWORKFLOW: Run completion tasks + // + PIPELINE_COMPLETION ( + params.email, + params.email_on_fail, + params.plaintext_email, + params.outdir, + params.monochrome_logs, + params.hook_url, + NFCORE_PANGENOME.out.multiqc_report + ) } /* diff --git a/modules.json b/modules.json index 07b78a01..5a0b94a9 100644 --- a/modules.json +++ b/modules.json @@ -93,7 +93,23 @@ } }, "subworkflows": { - "nf-core": {} + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + } + } } } } diff --git a/subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf b/subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf new file mode 100644 index 00000000..fc6e3f4b --- /dev/null +++ b/subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf @@ -0,0 +1,472 @@ +// +// Subworkflow with functionality specific to the nf-core/rnaseq pipeline +// + +import groovy.json.JsonSlurper + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' +include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' +include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' +include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' +include { imNotification } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' +include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE +======================================================================================== +*/ + +workflow PIPELINE_INITIALISATION { + + take: + version // boolean: Display version and exit + help // boolean: Display help text + validate_params // boolean: Boolean whether to validate parameters against the schema at runtime + monochrome_logs // boolean: Do not use coloured log outputs + nextflow_cli_args // array: List of positional nextflow CLI args + outdir // string: The output directory where the results will be saved + + main: + + // + // Print version and exit if required and dump pipeline parameters to JSON file + // + UTILS_NEXTFLOW_PIPELINE ( + version, + true, + outdir, + workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 + ) + + // + // Validate parameters and generate parameter summary to stdout + // + pre_help_text = nfCoreLogo(monochrome_logs) + post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) + def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input --n_haplotypes --outdir " + UTILS_NFVALIDATION_PLUGIN ( + help, + workflow_command, + pre_help_text, + post_help_text, + validate_params, + "nextflow_schema.json" + ) + + // + // Check config provided to the pipeline + // + UTILS_NFCORE_PIPELINE ( + nextflow_cli_args + ) + + // + // Custom validation for pipeline parameters + // + validateInputParameters() + +} + +/* +======================================================================================== + SUBWORKFLOW FOR PIPELINE COMPLETION +======================================================================================== +*/ + +workflow PIPELINE_COMPLETION { + + take: + email // string: email address + email_on_fail // string: email address sent on pipeline failure + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output + hook_url // string: hook URL for notifications + multiqc_report // string: Path to MultiQC report + + main: + + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + + // + // Completion email and summary + // + workflow.onComplete { + if (email || email_on_fail) { + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + } + + completionSummary(monochrome_logs) + + if (hook_url) { + imNotification(summary_params, hook_url) + } + } +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Function to validate channels from input samplesheet +// +def validateInputSamplesheet(input) { + def (metas, fastqs) = input[1..2] + + // Check that multiple runs of the same sample are of the same strandedness + def strandedness_ok = metas.collect{ it.strandedness }.unique().size == 1 + if (!strandedness_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must have the same strandedness!: ${metas[0].id}") + } + + // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + if (!endedness_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") + } + + return [ metas[0], fastqs ] +} + +// +// Check and validate pipeline parameters +// +def validateInputParameters() { + // Check mandatory parameters + if (!params.input) { + exit 1, 'Input FASTA not specified!' + } +} + +// +// Get attribute from genome config file e.g. fasta +// +def getGenomeAttribute(attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null +} + +// +// Exit pipeline if incorrect --genome key provided +// +def genomeExistsError() { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } +} + +// +// Generate methods description for MultiQC +// +def toolCitationText() { + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text +} + +def toolBibliographyText() { + // TODO nf-core: Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text +} + +def methodsDescriptionText(mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = workflow.toMap() + meta["manifest_map"] = workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + // meta["tool_bibliography"] = toolBibliographyText() + def methods_text = mqc_methods_yaml.text + + def engine = new groovy.text.SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html.toString() +} + +// +// Print a warning if both GTF and GFF have been provided +// +def gtfGffWarn() { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Both '--gtf' and '--gff' parameters have been provided.\n" + + " Using GTF file as priority.\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" +} + +// +// Print a warning if using GRCh38 assembly from igenomes.config +// +def ncbiGenomeWarn() { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " When using '--genome GRCh38' the assembly is from the NCBI and NOT Ensembl.\n" + + " Biotype QC will be skipped to circumvent the issue below:\n" + + " https://github.com/nf-core/rnaseq/issues/460\n\n" + + " If you would like to use the soft-masked Ensembl assembly instead please see:\n" + + " https://github.com/nf-core/rnaseq/issues/159#issuecomment-501184312\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" +} + +// +// Print a warning if using a UCSC assembly from igenomes.config +// +def ucscGenomeWarn() { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " When using UCSC assemblies the 'gene_biotype' field is absent from the GTF file.\n" + + " Biotype QC will be skipped to circumvent the issue below:\n" + + " https://github.com/nf-core/rnaseq/issues/460\n\n" + + " If you would like to use the soft-masked Ensembl assembly instead please see:\n" + + " https://github.com/nf-core/rnaseq/issues/159#issuecomment-501184312\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" +} + +// +// Print a warning if using '--transcript_fasta' +// +def transcriptsFastaWarn() { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " '--transcript_fasta' parameter has been provided.\n" + + " Make sure transcript names in this file match those in the GFF/GTF file.\n\n" + + " Please see:\n" + + " https://github.com/nf-core/rnaseq/issues/753\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" +} + +// +// Print a warning if --skip_alignment has been provided +// +def skipAlignmentWarn() { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " '--skip_alignment' parameter has been provided.\n" + + " Skipping alignment, genome-based quantification and all downstream QC processes.\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" +} + +// +// Print a warning if using '--aligner star_rsem' and '--with_umi' +// +def rsemUmiError() { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " When using '--aligner star_rsem', STAR is run by RSEM itself and so it is\n" + + " not possible to remove UMIs before the quantification.\n\n" + + " If you would like to remove UMI barcodes using the '--with_umi' option\n" + + " please use either '--aligner star_salmon' or '--aligner hisat2'.\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) +} + +// +// Print a warning if using '--aligner star_rsem' and providing both '--rsem_index' and '--star_index' +// +def rsemStarIndexWarn() { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " When using '--aligner star_rsem', both the STAR and RSEM indices should\n" + + " be present in the path specified by '--rsem_index'.\n\n" + + " This warning has been generated because you have provided both\n" + + " '--rsem_index' and '--star_index'. The pipeline will ignore the latter.\n\n" + + " Please see:\n" + + " https://github.com/nf-core/rnaseq/issues/568\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" +} + +// +// Print a warning if using '--aligner star_rsem' and providing '--star_extra_alignment_args' +// +def rsemStarExtraArgumentsWarn() { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " No additional arguments can be passed to STAR when using RSEM.\n" + + " Because RSEM enforces its own parameters for STAR, any extra arguments\n" + + " to STAR will be ignored. Alternatively, choose the STAR+Salmon route.\n\n" + + " This warning has been generated because you have provided both\n" + + " '--aligner star_rsem' and '--extra_star_align_args'.\n\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" +} + +// +// Print a warning if using '--additional_fasta' and '--_index' +// +def additionaFastaIndexWarn(index) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " When using '--additional_fasta ' the aligner index will not\n" + + " be re-built with the transgenes incorporated by default since you have \n" + + " already provided an index via '--${index}_index '.\n\n" + + " Set '--additional_fasta --${index}_index false --gene_bed false --save_reference'\n" + + " to re-build the index with transgenes included and the index and gene BED file will be saved in\n" + + " 'results/genome/index/${index}/' for re-use with '--${index}_index'.\n\n" + + " Ignore this warning if you know that the index already contains transgenes.\n\n" + + " Please see:\n" + + " https://github.com/nf-core/rnaseq/issues/556\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" +} + +// +// Function to generate an error if contigs in genome fasta file > 512 Mbp +// +def checkMaxContigSize(fai_file) { + def max_size = 512000000 + fai_file.eachLine { line -> + def lspl = line.split('\t') + def chrom = lspl[0] + def size = lspl[1] + if (size.toInteger() > max_size) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Contig longer than ${max_size}bp found in reference genome!\n\n" + + " ${chrom}: ${size}\n\n" + + " Provide the '--bam_csi_index' parameter to use a CSI instead of BAI index.\n\n" + + " Please see:\n" + + " https://github.com/nf-core/rnaseq/issues/744\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } + } +} + +// +// Create MultiQC tsv custom content from a list of values +// +def multiqcTsvFromList(tsv_data, header) { + def tsv_string = "" + if (tsv_data.size() > 0) { + tsv_string += "${header.join('\t')}\n" + tsv_string += tsv_data.join('\n') + } + return tsv_string +} + +// +// Function that parses Salmon quant 'meta_info.json' output file to get inferred strandedness +// +def getSalmonInferredStrandedness(json_file) { + def lib_type = new JsonSlurper().parseText(json_file.text).get('library_types')[0] + def strandedness = 'reverse' + if (lib_type) { + if (lib_type in ['U', 'IU']) { + strandedness = 'unstranded' + } else if (lib_type in ['SF', 'ISF']) { + strandedness = 'forward' + } else if (lib_type in ['SR', 'ISR']) { + strandedness = 'reverse' + } + } + return strandedness +} + +// +// Function that parses and returns the alignment rate from the STAR log output +// +def getStarPercentMapped(params, align_log) { + def percent_aligned = 0 + def pattern = /Uniquely mapped reads %\s*\|\s*([\d\.]+)%/ + align_log.eachLine { line -> + def matcher = line =~ pattern + if (matcher) { + percent_aligned = matcher[0][1].toFloat() + } + } + + def pass = false + if (percent_aligned >= params.min_mapped_reads.toFloat()) { + pass = true + } + return [ percent_aligned, pass ] +} + +// +// Function to check whether biotype field exists in GTF file +// +def biotypeInGtf(gtf_file, biotype) { + def hits = 0 + gtf_file.eachLine { line -> + def attributes = line.split('\t')[-1].split() + if (attributes.contains(biotype)) { + hits += 1 + } + } + if (hits) { + return true + } else { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Biotype attribute '${biotype}' not found in the last column of the GTF file!\n\n" + + " Biotype QC will be skipped to circumvent the issue below:\n" + + " https://github.com/nf-core/rnaseq/issues/460\n\n" + + " Amend '--featurecounts_group_type' to change this behaviour.\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + return false + } +} + +// +// Function that parses and returns the predicted strandedness from the RSeQC infer_experiment.py output +// +def getInferexperimentStrandedness(inferexperiment_file, cutoff=30) { + def sense = 0 + def antisense = 0 + def undetermined = 0 + inferexperiment_file.eachLine { line -> + def undetermined_matcher = line =~ /Fraction of reads failed to determine:\s([\d\.]+)/ + def se_sense_matcher = line =~ /Fraction of reads explained by "\++,--":\s([\d\.]+)/ + def se_antisense_matcher = line =~ /Fraction of reads explained by "\+-,-\+":\s([\d\.]+)/ + def pe_sense_matcher = line =~ /Fraction of reads explained by "1\++,1--,2\+-,2-\+":\s([\d\.]+)/ + def pe_antisense_matcher = line =~ /Fraction of reads explained by "1\+-,1-\+,2\+\+,2--":\s([\d\.]+)/ + if (undetermined_matcher) undetermined = undetermined_matcher[0][1].toFloat() * 100 + if (se_sense_matcher) sense = se_sense_matcher[0][1].toFloat() * 100 + if (se_antisense_matcher) antisense = se_antisense_matcher[0][1].toFloat() * 100 + if (pe_sense_matcher) sense = pe_sense_matcher[0][1].toFloat() * 100 + if (pe_antisense_matcher) antisense = pe_antisense_matcher[0][1].toFloat() * 100 + } + def strandedness = 'unstranded' + if (sense >= 100-cutoff) { + strandedness = 'forward' + } else if (antisense >= 100-cutoff) { + strandedness = 'reverse' + } + return [ strandedness, sense, antisense, undetermined ] +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf new file mode 100644 index 00000000..ac31f28f --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -0,0 +1,126 @@ +// +// Subworkflow with functionality that may be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NEXTFLOW_PIPELINE { + + take: + print_version // boolean: print version + dump_parameters // boolean: dump parameters + outdir // path: base directory used to publish pipeline results + check_conda_channels // boolean: check conda channels + + main: + + // + // Print workflow version and exit on --version + // + if (print_version) { + log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + System.exit(0) + } + + // + // Dump pipeline parameters to a JSON file + // + if (dump_parameters && outdir) { + dumpParametersToJSON(outdir) + } + + // + // When running with Conda, warn if channels have not been set-up appropriately + // + if (check_conda_channels) { + checkCondaChannels() + } + + emit: + dummy_emit = true +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Generate version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Dump pipeline parameters to a JSON file +// +def dumpParametersToJSON(outdir) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() +} + +// +// When running with -profile conda, warn if channels have not been set-up appropriately +// +def checkCondaChannels() { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml new file mode 100644 index 00000000..e5c3a0a8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml @@ -0,0 +1,38 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NEXTFLOW_PIPELINE" +description: Subworkflow with functionality that may be useful for any Nextflow pipeline +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - print_version: + type: boolean + description: | + Print the version of the pipeline and exit + - dump_parameters: + type: boolean + description: | + Dump the parameters of the pipeline to a JSON file + - output_directory: + type: directory + description: Path to output dir to write JSON file to. + pattern: "results/" + - check_conda_channel: + type: boolean + description: | + Check if the conda channel priority is correct. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" + - "@drpatelh" +maintainers: + - "@adamrtalbot" + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..68718e4f --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -0,0 +1,54 @@ + +nextflow_function { + + name "Test Functions" + script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Test Function getWorkflowVersion") { + + function "getWorkflowVersion" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dumpParametersToJSON") { + + function "dumpParametersToJSON" + + when { + function { + """ + // define inputs of the function here. Example: + input[0] = "$outputDir" + """.stripIndent() + } + } + + then { + assertAll( + { assert function.success } + ) + } + } + + test("Test Function checkCondaChannels") { + + function "checkCondaChannels" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..db2030f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,12 @@ +{ + "Test Function getWorkflowVersion": { + "content": [ + "v9.9.9" + ], + "timestamp": "2024-01-19T11:32:36.031083" + }, + "Test Function checkCondaChannels": { + "content": null, + "timestamp": "2024-01-19T11:32:50.456" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..f7c54bc6 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,123 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NEXTFLOW_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + workflow "UTILS_NEXTFLOW_PIPELINE" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Should run no inputs") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = false + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should print version") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = true + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } + ) + } + } + + test("Should dump params") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = params.outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should not create params JSON if no output directory") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = null + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config new file mode 100644 index 00000000..d0a926bf --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml new file mode 100644 index 00000000..f8476112 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nextflow_pipeline: + - subworkflows/nf-core/utils_nextflow_pipeline/** diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf new file mode 100644 index 00000000..a8b55d6f --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -0,0 +1,440 @@ +// +// Subworkflow with utility functions specific to the nf-core pipeline template +// + +import org.yaml.snakeyaml.Yaml +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFCORE_PIPELINE { + + take: + nextflow_cli_args + + main: + valid_config = checkConfigProvided() + checkProfileProvided(nextflow_cli_args) + + emit: + valid_config +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Warn if a -profile or Nextflow config has not been provided to run the pipeline +// +def checkConfigProvided() { + valid_config = true + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + valid_config = false + } + return valid_config +} + +// +// Exit pipeline if --profile contains spaces +// +def checkProfileProvided(nextflow_cli_args) { + if (workflow.profile.endsWith(',')) { + error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } + if (nextflow_cli_args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } +} + +// +// Citation string for pipeline +// +def workflowCitation() { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " ${workflow.manifest.doi}\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" +} + +// +// Generate workflow version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Get software versions for pipeline +// +def processVersionsFromYAML(yaml_file) { + Yaml yaml = new Yaml() + versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + return yaml.dumpAsMap(versions).trim() +} + +// +// Get workflow version for pipeline +// +def workflowVersionToYAML() { + return """ + Workflow: + $workflow.manifest.name: ${getWorkflowVersion()} + Nextflow: $workflow.nextflow.version + """.stripIndent().trim() +} + +// +// Get channel of software versions used in pipeline in YAML format +// +def softwareVersionsToYAML(ch_versions) { + return ch_versions + .unique() + .map { processVersionsFromYAML(it) } + .unique() + .mix(Channel.of(workflowVersionToYAML())) +} + +// +// Get workflow summary for MultiQC +// +def paramsSummaryMultiqc(summary_params) { + def summary_section = '' + for (group in summary_params.keySet()) { + def group_params = summary_params.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + + return yaml_file_text +} + +// +// nf-core logo +// +def nfCoreLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + +// +// Return dashed line +// +def dashedLine(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" +} + +// +// ANSII colours used for terminal logging +// +def logColours(monochrome_logs=true) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes +} + +// +// Attach the multiqc report to email +// +def attachMultiqcReport(multiqc_report) { + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + return mqc_report +} + +// +// Construct and send completion email +// +def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = getWorkflowVersion() + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = attachMultiqcReport(multiqc_report) + + // Check if we are only sending emails on failure + def email_address = email + if (!email && email_on_fail && !workflow.success) { + email_address = email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("${workflow.projectDir}/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("${workflow.projectDir}/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(monochrome_logs) + if (email_address) { + try { + if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() +} + +// +// Print pipeline summary on completion +// +def completionSummary(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } +} + +// +// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack +// +def imNotification(summary_params, hook_url) { + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = getWorkflowVersion() + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("${workflow.projectDir}/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml new file mode 100644 index 00000000..d08d2434 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml @@ -0,0 +1,24 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFCORE_PIPELINE" +description: Subworkflow with utility functions specific to the nf-core pipeline template +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - nextflow_cli_args: + type: list + description: | + Nextflow CLI positional arguments +output: + - success: + type: boolean + description: | + Dummy output to indicate success +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..1dc317f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -0,0 +1,134 @@ + +nextflow_function { + + name "Test Functions" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Test Function checkConfigProvided") { + + function "checkConfigProvided" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function checkProfileProvided") { + + function "checkProfileProvided" + + when { + function { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function workflowCitation") { + + function "workflowCitation" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function nfCoreLogo") { + + function "nfCoreLogo" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dashedLine") { + + function "dashedLine" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function without logColours") { + + function "logColours" + + when { + function { + """ + input[0] = true + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function with logColours") { + function "logColours" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..10f948e6 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,138 @@ +{ + "Test Function checkProfileProvided": { + "content": null, + "timestamp": "2024-02-09T15:43:55.145717" + }, + "Test Function checkConfigProvided": { + "content": [ + true + ], + "timestamp": "2024-01-19T11:34:13.548431224" + }, + "Test Function nfCoreLogo": { + "content": [ + "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" + ], + "timestamp": "2024-01-19T11:34:38.840454873" + }, + "Test Function workflowCitation": { + "content": [ + "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" + ], + "timestamp": "2024-01-19T11:34:22.24352016" + }, + "Test Function without logColours": { + "content": [ + { + "reset": "", + "bold": "", + "dim": "", + "underlined": "", + "blink": "", + "reverse": "", + "hidden": "", + "black": "", + "red": "", + "green": "", + "yellow": "", + "blue": "", + "purple": "", + "cyan": "", + "white": "", + "bblack": "", + "bred": "", + "bgreen": "", + "byellow": "", + "bblue": "", + "bpurple": "", + "bcyan": "", + "bwhite": "", + "ublack": "", + "ured": "", + "ugreen": "", + "uyellow": "", + "ublue": "", + "upurple": "", + "ucyan": "", + "uwhite": "", + "iblack": "", + "ired": "", + "igreen": "", + "iyellow": "", + "iblue": "", + "ipurple": "", + "icyan": "", + "iwhite": "", + "biblack": "", + "bired": "", + "bigreen": "", + "biyellow": "", + "biblue": "", + "bipurple": "", + "bicyan": "", + "biwhite": "" + } + ], + "timestamp": "2024-01-19T11:35:04.418416984" + }, + "Test Function dashedLine": { + "content": [ + "-\u001b[2m----------------------------------------------------\u001b[0m-" + ], + "timestamp": "2024-01-19T11:34:55.420000755" + }, + "Test Function with logColours": { + "content": [ + { + "reset": "\u001b[0m", + "bold": "\u001b[1m", + "dim": "\u001b[2m", + "underlined": "\u001b[4m", + "blink": "\u001b[5m", + "reverse": "\u001b[7m", + "hidden": "\u001b[8m", + "black": "\u001b[0;30m", + "red": "\u001b[0;31m", + "green": "\u001b[0;32m", + "yellow": "\u001b[0;33m", + "blue": "\u001b[0;34m", + "purple": "\u001b[0;35m", + "cyan": "\u001b[0;36m", + "white": "\u001b[0;37m", + "bblack": "\u001b[1;30m", + "bred": "\u001b[1;31m", + "bgreen": "\u001b[1;32m", + "byellow": "\u001b[1;33m", + "bblue": "\u001b[1;34m", + "bpurple": "\u001b[1;35m", + "bcyan": "\u001b[1;36m", + "bwhite": "\u001b[1;37m", + "ublack": "\u001b[4;30m", + "ured": "\u001b[4;31m", + "ugreen": "\u001b[4;32m", + "uyellow": "\u001b[4;33m", + "ublue": "\u001b[4;34m", + "upurple": "\u001b[4;35m", + "ucyan": "\u001b[4;36m", + "uwhite": "\u001b[4;37m", + "iblack": "\u001b[0;90m", + "ired": "\u001b[0;91m", + "igreen": "\u001b[0;92m", + "iyellow": "\u001b[0;93m", + "iblue": "\u001b[0;94m", + "ipurple": "\u001b[0;95m", + "icyan": "\u001b[0;96m", + "iwhite": "\u001b[0;97m", + "biblack": "\u001b[1;90m", + "bired": "\u001b[1;91m", + "bigreen": "\u001b[1;92m", + "biyellow": "\u001b[1;93m", + "biblue": "\u001b[1;94m", + "bipurple": "\u001b[1;95m", + "bicyan": "\u001b[1;96m", + "biwhite": "\u001b[1;97m" + } + ], + "timestamp": "2024-01-19T11:35:13.436366565" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..8940d32d --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,29 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFCORE_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + workflow "UTILS_NFCORE_PIPELINE" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Should run without failures") { + + when { + workflow { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap new file mode 100644 index 00000000..d07ce54c --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -0,0 +1,15 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + true + ], + "valid_config": [ + true + ] + } + ], + "timestamp": "2024-01-19T11:35:22.538940073" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config new file mode 100644 index 00000000..d0a926bf --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml new file mode 100644 index 00000000..ac8523c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfcore_pipeline: + - subworkflows/nf-core/utils_nfcore_pipeline/** diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf new file mode 100644 index 00000000..2585b65d --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf @@ -0,0 +1,62 @@ +// +// Subworkflow that uses the nf-validation plugin to render help text and parameter summary +// + +/* +======================================================================================== + IMPORT NF-VALIDATION PLUGIN +======================================================================================== +*/ + +include { paramsHelp } from 'plugin/nf-validation' +include { paramsSummaryLog } from 'plugin/nf-validation' +include { validateParameters } from 'plugin/nf-validation' + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFVALIDATION_PLUGIN { + + take: + print_help // boolean: print help + workflow_command // string: default commmand used to run pipeline + pre_help_text // string: string to be printed before help text and summary log + post_help_text // string: string to be printed after help text and summary log + validate_params // boolean: validate parameters + schema_filename // path: JSON schema file, null to use default value + + main: + + log.debug "Using schema file: ${schema_filename}" + + // Default values for strings + pre_help_text = pre_help_text ?: '' + post_help_text = post_help_text ?: '' + workflow_command = workflow_command ?: '' + + // + // Print help message if needed + // + if (print_help) { + log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text + System.exit(0) + } + + // + // Print parameter summary to stdout + // + log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text + + // + // Validate parameters relative to the parameter JSON schema + // + if (validate_params){ + validateParameters(parameters_schema: schema_filename) + } + + emit: + dummy_emit = true +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml new file mode 100644 index 00000000..3d4a6b04 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFVALIDATION_PLUGIN" +description: Use nf-validation to initiate and validate a pipeline +keywords: + - utility + - pipeline + - initialise + - validation +components: [] +input: + - print_help: + type: boolean + description: | + Print help message and exit + - workflow_command: + type: string + description: | + The command to run the workflow e.g. "nextflow run main.nf" + - pre_help_text: + type: string + description: | + Text to print before the help message + - post_help_text: + type: string + description: | + Text to print after the help message + - validate_params: + type: boolean + description: | + Validate the parameters and error if invalid. + - schema_filename: + type: string + description: | + The filename of the schema to validate against. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test new file mode 100644 index 00000000..5784a33f --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -0,0 +1,200 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFVALIDATION_PLUGIN" + script "../main.nf" + workflow "UTILS_NFVALIDATION_PLUGIN" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "plugin/nf-validation" + tag "'plugin/nf-validation'" + tag "utils_nfvalidation_plugin" + tag "subworkflows/utils_nfvalidation_plugin" + + test("Should run nothing") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should run help") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with command") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with extra text") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = "pre-help-text" + post_help_text = "post-help-text" + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('pre-help-text') } }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } }, + { assert workflow.stdout.any { it.contains('post-help-text') } } + ) + } + } + + test("Should validate params") { + + when { + + params { + monochrome_logs = true + test_data = '' + outdir = 1 + } + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = true + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json new file mode 100644 index 00000000..7626c1c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json @@ -0,0 +1,96 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", + "title": ". pipeline parameters", + "description": "", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["outdir"], + "properties": { + "validate_params": { + "type": "boolean", + "description": "Validate parameters?", + "default": true, + "hidden": true + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules", + "description": "Base for test data directory", + "hidden": true + }, + "test_data": { + "type": "string", + "description": "Fake test data param", + "hidden": true + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "logo": { + "type": "boolean", + "default": true, + "description": "Display nf-core logo in console output.", + "fa_icon": "fas fa-image", + "hidden": true + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Pull Singularity container from Docker?", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Use monochrome_logs", + "hidden": true + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml new file mode 100644 index 00000000..60b1cfff --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfvalidation_plugin: + - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/workflows/pangenome.nf b/workflows/pangenome.nf index 5ebc16fa..525fa506 100644 --- a/workflows/pangenome.nf +++ b/workflows/pangenome.nf @@ -4,32 +4,9 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' - -def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) -def citation = '\n' + WorkflowMain.citation(workflow) + '\n' -def summary_params = paramsSummaryMap(workflow) - -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - -// Check input path parameters to see if they exist -def checkPathParamList = [ params.input, params.multiqc_config ] -for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } - -// Check mandatory parameters -if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input FASTA not specified!' } - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -56,7 +33,6 @@ include { ODGI_QC } from '../subworkflows/local/odgi_qc' // MODULE: Installed directly from nf-core/modules // include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' include { ODGI_SQUEEZE } from '../modules/nf-core/odgi/squeeze/main' include { ODGI_VIEW } from '../modules/nf-core/odgi/view/main' @@ -72,23 +48,20 @@ include { MULTIQC_COMMUNITY } from '../modules/local/multiqc_community ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// Info required for completion email and summary -def multiqc_report = [] - workflow PANGENOME { + main: + ch_versions = Channel.empty() + ch_multiqc_report = Channel.empty() // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files + // SUBWORKFLOW: Read in FASTA, validate and generate appropriate indices // INPUT_CHECK ( - file(params.input) + file(params.input, checkIfExists: true) ) ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema if (params.communities) { COMMUNITY ( @@ -140,23 +113,21 @@ workflow PANGENOME { } - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique().collectFile(name: 'collated_versions.yml') - ) + softwareVersionsToYAML(ch_versions) + .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pangenome_software_mqc_versions.yml', sort: true, newLine: true) + .set { ch_collated_versions } // // MODULE: MultiQC // - workflow_summary = WorkflowPangenome.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowPangenome.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) - + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo) : Channel.empty() + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) ch_multiqc_files = Channel.empty() ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) if (!params.communities) { if (!params.wfmash_only) { ch_multiqc_files = ch_multiqc_files.mix(PGGB.out.qc.map{return it[1..8]}) @@ -175,31 +146,11 @@ workflow PANGENOME { ch_multiqc_custom_config.toList(), ch_multiqc_logo.toList() ) - multiqc_report = MULTIQC.out.report.toList() -} + ch_multiqc_report = MULTIQC.out.report -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.dump_parameters(workflow, params) - NfcoreTemplate.summary(workflow, params, log) - if (params.hook_url) { - NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) - } -} - -workflow.onError { - if (workflow.errorReport.contains("Process requirement exceeds available memory")) { - println("🛑 Default resources exceed availability 🛑 ") - println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") - } + emit: + multiqc_report = ch_multiqc_report // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [version1, version2, ...] } /* From 53b3fb363a2ba5ab6cb644527aa0b98ba7fe241e Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 26 Feb 2024 14:59:36 +0100 Subject: [PATCH 61/83] deleted all lib files --- lib/NfcoreTemplate.groovy | 356 ----------------------------------- lib/Utils.groovy | 47 ----- lib/WorkflowMain.groovy | 64 ------- lib/WorkflowPangenome.groovy | 103 ---------- 4 files changed, 570 deletions(-) delete mode 100755 lib/NfcoreTemplate.groovy delete mode 100644 lib/Utils.groovy delete mode 100755 lib/WorkflowMain.groovy delete mode 100755 lib/WorkflowPangenome.groovy diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index e248e4c3..00000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,356 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Warn if a -profile or Nextflow config has not been provided to run the pipeline - // - public static void checkConfigProvided(workflow, log) { - if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " - } - } - - // - // Generate version string - // - public static String version(workflow) { - String version_string = "" - - if (workflow.manifest.version) { - def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' - version_string += "${prefix_v}${workflow.manifest.version}" - } - - if (workflow.commitId) { - def git_shortsha = workflow.commitId.substring(0, 7) - version_string += "-g${git_shortsha}" - } - - return version_string - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = NfcoreTemplate.version(workflow) - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" - } - mqc_report = mqc_report[0] - } - } - } catch (all) { - if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" - } - } - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") - sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); - output_hf.delete() - - // Write summary e-mail TXT to a file - def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); - output_tf.delete() - } - - // - // Construct and send a notification to a web server as JSON - // e.g. Microsoft Teams and Slack - // - public static void IM_notification(workflow, params, summary_params, projectDir, log) { - def hook_url = params.hook_url - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp - - def msg_fields = [:] - msg_fields['version'] = NfcoreTemplate.version(workflow) - msg_fields['runName'] = workflow.runName - msg_fields['success'] = workflow.success - msg_fields['dateComplete'] = workflow.complete - msg_fields['duration'] = workflow.duration - msg_fields['exitStatus'] = workflow.exitStatus - msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") - msg_fields['projectDir'] = workflow.projectDir - msg_fields['summary'] = summary << misc_fields - - // Render the JSON template - def engine = new groovy.text.GStringTemplateEngine() - // Different JSON depending on the service provider - // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format - def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" - def hf = new File("$projectDir/assets/${json_path}") - def json_template = engine.createTemplate(hf).make(msg_fields) - def json_message = json_template.toString() - - // POST - def post = new URL(hook_url).openConnection(); - post.setRequestMethod("POST") - post.setDoOutput(true) - post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); - } - } - - // - // Dump pipeline parameters in a json file - // - public static void dump_parameters(workflow, params) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) - - FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") - temp_pf.delete() - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // nf-core logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String workflow_version = NfcoreTemplate.version(workflow) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100644 index 8d030f4e..00000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] - def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean - - // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } - - if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - } - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index d9afc927..00000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,64 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the nf-core/pangenome pipeline -// - -import nextflow.Nextflow - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - "* The pipeline\n" + - " https://doi.org/10.5281/zenodo.8202636\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log, args) { - - // Print workflow version and exit on --version - if (params.version) { - String workflow_version = NfcoreTemplate.version(workflow) - log.info "${workflow.manifest.name} ${workflow_version}" - System.exit(0) - } - - // Check that a -profile or Nextflow config has been provided to run the pipeline - NfcoreTemplate.checkConfigProvided(workflow, log) - // Check that the profile doesn't contain spaces and doesn't end with a trailing comma - checkProfile(workflow.profile, args, log) - - // Check that conda channels are set-up correctly - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - - // Check input has been provided - if (!params.input) { - error("Please provide an input FASTA to the pipeline e.g. '--input sequences.fa.gz'") - } - } - - // - // Exit pipeline if --profile contains spaces - // - private static void checkProfile(profile, args, log) { - if (profile.endsWith(',')) { - Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - if (args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - } -} diff --git a/lib/WorkflowPangenome.groovy b/lib/WorkflowPangenome.groovy deleted file mode 100755 index f85a06fb..00000000 --- a/lib/WorkflowPangenome.groovy +++ /dev/null @@ -1,103 +0,0 @@ -// -// This file holds several functions specific to the workflow/pangenome.nf in the nf-core/pangenome pipeline -// - -import nextflow.Nextflow -import groovy.text.SimpleTemplateEngine - -class WorkflowPangenome { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - - // This function is currently not in use - } - - // - // Get workflow summary for MultiQC - // - public static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

    $group

    \n" - summary_section += "
    \n" - for (param in group_params.keySet()) { - summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" - } - summary_section += "
    \n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } - - // - // Generate methods description for MultiQC - // - - public static String toolCitationText(params) { - - // TODO nf-core: Optionally add in-text citation tools to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def citation_text = [ - "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." - ].join(' ').trim() - - return citation_text - } - - public static String toolBibliographyText(params) { - - // TODO Optionally add bibliographic entries to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " - ].join(' ').trim() - - return reference_text - } - - public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { - // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file - def meta = [:] - meta.workflow = run_workflow.toMap() - meta["manifest_map"] = run_workflow.manifest.toMap() - - // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " - - // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - //meta["tool_bibliography"] = toolBibliographyText(params) - - - def methods_text = mqc_methods_yaml.text - - def engine = new SimpleTemplateEngine() - def description_html = engine.createTemplate(methods_text).make(meta) - - return description_html - } -} From 28f2a11d23b645e4f30e2c1dcdff8feb3e912d6e Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 26 Feb 2024 15:12:20 +0100 Subject: [PATCH 62/83] fix seqwish multiqc --- subworkflows/local/odgi_qc.nf | 5 ++++- 1 file changed, 4 insertions(+), 1 deletion(-) diff --git a/subworkflows/local/odgi_qc.nf b/subworkflows/local/odgi_qc.nf index 967f5111..86d34062 100644 --- a/subworkflows/local/odgi_qc.nf +++ b/subworkflows/local/odgi_qc.nf @@ -25,6 +25,7 @@ workflow ODGI_QC { ch_graph_qc = Channel.empty() // we collect all graph quality control PNGs here ODGI_STATS(seqwish.mix(sorted_graph)) + ODGI_STATS.out.yaml.collect().view() ch_versions = ch_versions.mix(ODGI_STATS.out.versions) // prepare all inputs ch_odgi_viz = sorted_graph.map{meta, gfa -> [ [ id: meta.id + ".viz" ], gfa ]} @@ -63,7 +64,7 @@ workflow ODGI_QC { ch_graph_qc = ch_graph_qc.join(ODGI_VIZ_UNCALLED.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".gfaffix.*", "") ], png ]}) ch_graph_qc = ch_graph_qc.join(ODGI_DRAW_MULTIQC.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".gfaffix.*", "") ], png ]}) } else { - ch_graph_qc = ODGI_STATS.out.yaml + ch_graph_qc = ODGI_STATS.out.yaml.collect().map{seqwish_id, seqwish_qc, gfaffix_id, gfaffix_qc -> [ gfaffix_id, gfaffix_qc, seqwish_qc ]} ch_graph_qc = ch_graph_qc.join(ODGI_VIZ_COLOR.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".viz", "") ], png ]}) ch_graph_qc = ch_graph_qc.join(ODGI_VIZ_POS.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".viz_pos", "") ], png ]}) ch_graph_qc = ch_graph_qc.join(ODGI_VIZ_DEPTH.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".viz_depth", "") ], png ]}) @@ -73,6 +74,8 @@ workflow ODGI_QC { ch_graph_qc = ch_graph_qc.join(ODGI_DRAW_MULTIQC.out.png) } + ch_graph_qc.view() + emit: qc = ch_graph_qc // [ seqwish.og.stats.yaml , gfaffix.og.stats.yaml, odgi_viz_multiqc.png, odgi_viz_pos_multiqc.png, odgi_viz_depth_multiqc.png, odgi_viz_inv_multiqc.png, odgi_viz_compr_multiqc.png, odgi_viz_uncalled_multiqc.png, odgi_draw_multiqc.png ] versions = ch_versions // channel: [ versions.yml ] From e61e23017097ca35ed124b4466a8be4a0e7876f6 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 26 Feb 2024 15:15:39 +0100 Subject: [PATCH 63/83] add Peter Heringer to the contributors --- README.md | 1 + 1 file changed, 1 insertion(+) diff --git a/README.md b/README.md index a0fa477c..26cc9686 100644 --- a/README.md +++ b/README.md @@ -81,6 +81,7 @@ Many thanks to all who have helped out and contributed along the way, including | [Erik Garrison](https://github.com/ekg) | [The University of Tennessee Health Science Center, Memphis, Tennessee, TN, USA](https://uthsc.edu/) | | [Andrea Guarracino](https://github.com/AndreaGuarracino) | [Genomics Research Centre, Human Technopole, Milan, Italy](https://humantechnopole.it/en/)
    [The University of Tennessee Health Science Center, Memphis, Tennessee, TN, USA](https://uthsc.edu/) | | [Friederike Hanssen](https://github.com/FriederikeHanssen) | [Quantitative Biology Center (QBiC) Tübingen, University of Tübingen, Germany](https://uni-tuebingen.de/en/research/research-infrastructure/quantitative-biology-center-qbic/)
    [Biomedical Data Science, Department of Computer Science, University of Tübingen, Germany](https://uni-tuebingen.de/en/faculties/faculty-of-science/departments/computer-science/department/) | +| [Peter Heringer](https://github.com/heringerp) | [Quantitative Biology Center (QBiC) Tübingen, University of Tübingen, Germany](https://uni-tuebingen.de/en/research/research-infrastructure/quantitative-biology-center-qbic/)
    [Biomedical Data Science, Department of Computer Science, University of Tübingen, Germany](https://uni-tuebingen.de/en/faculties/faculty-of-science/departments/computer-science/department/) | | [Michael Heuer](https://github.com/heuermh) | [Mammoth Biosciences, Inc., San Francisco, CA, USA](https://mammoth.bio) | | [Lukas Heumos](https://github.com/zethson) | [Institute of Computational Biology, Helmholtz Zentrum München, Munich, Germany](https://www.helmholtz-muenchen.de/icb/index.html)
    [Institute of Lung Biology and Disease and Comprehensive Pneumology Center, Helmholtz Zentrum München, Munich, Germany](https://www.helmholtz-muenchen.de/ilbd/the-institute/cpc/index.html) | | [Simon Heumos](https://github.com/subwaystation) | [Quantitative Biology Center (QBiC) Tübingen, University of Tübingen, Germany](https://uni-tuebingen.de/en/research/research-infrastructure/quantitative-biology-center-qbic/)
    [Biomedical Data Science, Department of Computer Science, University of Tübingen, Germany](https://uni-tuebingen.de/en/faculties/faculty-of-science/departments/computer-science/department/) | From ce913173468548d12c4f8dbc99712b606ee1a658 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 26 Feb 2024 15:19:24 +0100 Subject: [PATCH 64/83] fix bugs --- workflows/pangenome.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/pangenome.nf b/workflows/pangenome.nf index 525fa506..69fcfc51 100644 --- a/workflows/pangenome.nf +++ b/workflows/pangenome.nf @@ -62,6 +62,7 @@ workflow PANGENOME { file(params.input, checkIfExists: true) ) ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) if (params.communities) { COMMUNITY ( @@ -120,7 +121,6 @@ workflow PANGENOME { // // MODULE: MultiQC // - ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo) : Channel.empty() summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") From 6e62f72eaa57c4a410801953b6de8ec92e0f200e Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 26 Feb 2024 15:21:37 +0100 Subject: [PATCH 65/83] fix more bugs --- workflows/pangenome.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/workflows/pangenome.nf b/workflows/pangenome.nf index 69fcfc51..b7e20c6e 100644 --- a/workflows/pangenome.nf +++ b/workflows/pangenome.nf @@ -63,6 +63,8 @@ workflow PANGENOME { ) ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo) : Channel.empty() if (params.communities) { COMMUNITY ( @@ -121,8 +123,6 @@ workflow PANGENOME { // // MODULE: MultiQC // - ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() - ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo) : Channel.empty() summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) ch_multiqc_files = Channel.empty() From 0cf6fc0e6673052bd6d26486bdda61cfd6563629 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Mon, 26 Feb 2024 16:05:10 +0100 Subject: [PATCH 66/83] fix me, I am a bug --- subworkflows/local/odgi_qc.nf | 17 ++++++++++------- subworkflows/local/pggb.nf | 3 ++- workflows/pangenome.nf | 6 ++++-- 3 files changed, 16 insertions(+), 10 deletions(-) diff --git a/subworkflows/local/odgi_qc.nf b/subworkflows/local/odgi_qc.nf index 86d34062..48720552 100644 --- a/subworkflows/local/odgi_qc.nf +++ b/subworkflows/local/odgi_qc.nf @@ -15,9 +15,10 @@ include { ODGI_DRAW as ODGI_DRAW_HEIGHT } from '../../modules/nf-core/odgi/dra workflow ODGI_QC { take: - seqwish // file: /path/to/seqwish.og - sorted_graph // file: /path/to/sorted_graph.og - community_mode // val : determines how to handly meta identifiers + seqwish // file: /path/to/seqwish.og + sorted_graph // file: /path/to/sorted_graph.og + community_mode // val : determines how to handly meta identifiers + no_seqwish_input // val : determiens how we will sort the input for MULTIQC main: @@ -25,7 +26,6 @@ workflow ODGI_QC { ch_graph_qc = Channel.empty() // we collect all graph quality control PNGs here ODGI_STATS(seqwish.mix(sorted_graph)) - ODGI_STATS.out.yaml.collect().view() ch_versions = ch_versions.mix(ODGI_STATS.out.versions) // prepare all inputs ch_odgi_viz = sorted_graph.map{meta, gfa -> [ [ id: meta.id + ".viz" ], gfa ]} @@ -64,7 +64,12 @@ workflow ODGI_QC { ch_graph_qc = ch_graph_qc.join(ODGI_VIZ_UNCALLED.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".gfaffix.*", "") ], png ]}) ch_graph_qc = ch_graph_qc.join(ODGI_DRAW_MULTIQC.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".gfaffix.*", "") ], png ]}) } else { - ch_graph_qc = ODGI_STATS.out.yaml.collect().map{seqwish_id, seqwish_qc, gfaffix_id, gfaffix_qc -> [ gfaffix_id, gfaffix_qc, seqwish_qc ]} + // + if (no_seqwish_input) { + ch_graph_qc = ODGI_STATS.out.yaml + } else { + ch_graph_qc = ODGI_STATS.out.yaml.collect().map{seqwish_id, seqwish_qc, gfaffix_id, gfaffix_qc -> [ gfaffix_id, gfaffix_qc, seqwish_qc ]} + } ch_graph_qc = ch_graph_qc.join(ODGI_VIZ_COLOR.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".viz", "") ], png ]}) ch_graph_qc = ch_graph_qc.join(ODGI_VIZ_POS.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".viz_pos", "") ], png ]}) ch_graph_qc = ch_graph_qc.join(ODGI_VIZ_DEPTH.out.png.map{meta, png -> [ [ id: meta.id.replaceFirst(".viz_depth", "") ], png ]}) @@ -74,8 +79,6 @@ workflow ODGI_QC { ch_graph_qc = ch_graph_qc.join(ODGI_DRAW_MULTIQC.out.png) } - ch_graph_qc.view() - emit: qc = ch_graph_qc // [ seqwish.og.stats.yaml , gfaffix.og.stats.yaml, odgi_viz_multiqc.png, odgi_viz_pos_multiqc.png, odgi_viz_depth_multiqc.png, odgi_viz_inv_multiqc.png, odgi_viz_compr_multiqc.png, odgi_viz_uncalled_multiqc.png, odgi_draw_multiqc.png ] versions = ch_versions // channel: [ versions.yml ] diff --git a/subworkflows/local/pggb.nf b/subworkflows/local/pggb.nf index 4ba4b932..20991fea 100644 --- a/subworkflows/local/pggb.nf +++ b/subworkflows/local/pggb.nf @@ -22,6 +22,7 @@ workflow PGGB { fai // file: /path/to/sequences.fasta.fai gzi // file: /path/to/sequences.fasta.gzi community_mode // val: determines how we will build our meta identifiers of ODGI_QC + no_seqwish_input // val : determiens how we will sort the input for MULTIQC main: @@ -129,7 +130,7 @@ workflow PGGB { ch_sorted_graph = ODGI_SORT.out.sorted_graph - ODGI_QC(ch_odgi_build_seqwish, ODGI_SORT.out.sorted_graph, community_mode) + ODGI_QC(ch_odgi_build_seqwish, ODGI_SORT.out.sorted_graph, community_mode, no_seqwish_input) ch_graph_qc = ODGI_QC.out.qc ch_versions = ch_versions.mix(ODGI_QC.out.versions) } diff --git a/workflows/pangenome.nf b/workflows/pangenome.nf index b7e20c6e..c7ed386f 100644 --- a/workflows/pangenome.nf +++ b/workflows/pangenome.nf @@ -78,13 +78,14 @@ workflow PANGENOME { ch_community_join.map{meta, fasta, gzi, fai -> [ meta, fasta ]}, ch_community_join.map{meta, fasta, gzi, fai -> [ meta, fai ]}, ch_community_join.map{meta, fasta, gzi, fai -> [ meta, gzi ]}, - true + true, + false ) ch_versions = ch_versions.mix(PGGB.out.versions) ch_squeeze_in = PGGB.out.og.map{meta, og -> [ [ id:meta.id.replaceFirst(".community.*", "") ], og ]}.groupTuple(by:0) ODGI_SQUEEZE(ch_squeeze_in) ch_odgi_qc_in = ODGI_SQUEEZE.out.graph.map{meta, ogs -> [ [ id:meta.id + ".squeeze" ], ogs ]} - ODGI_QC(Channel.empty(), ch_odgi_qc_in, false) + ODGI_QC(Channel.empty(), ch_odgi_qc_in, false, true) ch_versions = ch_versions.mix(ODGI_QC.out.versions) ch_versions = ch_versions.mix(ODGI_SQUEEZE.out.versions) ODGI_VIEW(ch_odgi_qc_in) @@ -99,6 +100,7 @@ workflow PANGENOME { INPUT_CHECK.out.fasta, INPUT_CHECK.out.fai, INPUT_CHECK.out.gzi, + false, false ) ch_versions = ch_versions.mix(PGGB.out.versions) From 5f2c926ea757738ffc57ab8ac34911f2cde83d66 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 27 Feb 2024 10:56:57 +0100 Subject: [PATCH 67/83] bug fix --- subworkflows/local/input_check.nf | 81 +++++++++++++++++++------------ 1 file changed, 51 insertions(+), 30 deletions(-) diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 0aecf87f..a0cfeb56 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -1,44 +1,65 @@ // -// Check input samplesheet and get read channels +// Check input FASTA and prepare indices // -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' +include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip/main.nf' +include { SAMTOOLS_FAIDX } from '../../modules/nf-core/samtools/faidx/main.nf' workflow INPUT_CHECK { take: - samplesheet // file: /path/to/samplesheet.csv + fasta // file: /path/to/sequences.fasta main: - SAMPLESHEET_CHECK ( samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } - emit: - reads // channel: [ val(meta), [ reads ] ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] -} + ch_versions = Channel.empty() // we collect all versions here + ch_fasta = Channel.empty() // final output channel [ val(meta) , [ fasta ] ] -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] + fai_path = file("${params.input}.fai") + gzi_path = file("${params.input}.gzi") + + fai = Channel.empty() // we store the .fai index here [ fai ] + gzi = Channel.empty() // we store the .gzi index here [ gzi ] + + meta_fasta = Channel.empty() // intermediate channel where we build our [ val(meta) , [ fasta ] ] + fasta_file_name = fasta.getName() + + if (params.input.endsWith(".gz")) { + meta_fasta = tuple([ id:fasta_file_name ], fasta) + // TODO We want to check, if the input file was actually compressed with bgzip with the upcoming grabix module. + // For now we assume it was bgzip. If not WFMASH will complain instantly anyhow. + if (!fai_path.exists() || !gzi_path.exists()) { // the assumption is that none of these files exist if only one does not exist + SAMTOOLS_FAIDX(meta_fasta, [[],[]]) + fai = SAMTOOLS_FAIDX.out.fai + gzi = SAMTOOLS_FAIDX.out.gzi + ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions) + } else { + fai = Channel.of([ [ id:fasta_file_name ], fai_path ]) + gzi = Channel.of([ [ id:fasta_file_name ], gzi_path ]) + } + ch_fasta = meta_fasta } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" + if (params.input.endsWith("fa")) { + fasta_file_name = fasta_file_name.substring(0, fasta_file_name.length() - 3) + } else { + if (params.input.endswith("fasta")) { + fasta_file_name = fasta_file_name.substring(0, fasta_file_name.length() - 6) + } else { // we assume "fna" here + fasta_file_name = fasta_file_name.substring(0, fasta_file_name.length() - 4) + } } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] + meta_fasta = tuple([ id:fasta_file_name ], fasta) + TABIX_BGZIP(meta_fasta) + ch_fasta = TABIX_BGZIP.out.output + SAMTOOLS_FAIDX(ch_fasta, [[],[]]) + gzi = SAMTOOLS_FAIDX.out.gzi + fai = SAMTOOLS_FAIDX.out.fai + ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions) + ch_versions = ch_versions.mix(TABIX_BGZIP.out.versions) } - return fastq_meta + + emit: + fasta = ch_fasta // channel: [ val(meta), [ fasta ] ] + fai = fai // channel: [ val(meta), fasta.fai ] + gzi = gzi // channel: [ val(meta), fasta.gzi ] + versions = ch_versions // channel: [ versions.yml ] } From d2c276b328c4eee1eb44e3235b9440bd4edca255 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 27 Feb 2024 10:57:58 +0100 Subject: [PATCH 68/83] remove customdumpsoftwareversions --- modules.json | 5 ----- 1 file changed, 5 deletions(-) diff --git a/modules.json b/modules.json index ba0eb1a8..814a1538 100644 --- a/modules.json +++ b/modules.json @@ -5,11 +5,6 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - }, "gfaffix": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", From c0386758112b0eefbb40f99b65725c38f57522b1 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 27 Feb 2024 11:00:59 +0100 Subject: [PATCH 69/83] hopefully final conflict resolved --- modules/nf-core/multiqc/tests/main.nf.test.snap | 12 ------------ 1 file changed, 12 deletions(-) diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index c2708200..c204b488 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -24,30 +24,18 @@ "nf-test": "0.8.4", "nextflow": "23.10.1" }, -<<<<<<< HEAD "timestamp": "2024-02-14T09:29:28.847433492" -======= - "timestamp": "2024-01-31T17:45:09.605359" ->>>>>>> dev }, "multiqc_versions_config": { "content": [ [ -<<<<<<< HEAD "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" -======= - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" ->>>>>>> dev ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, -<<<<<<< HEAD "timestamp": "2024-02-14T09:29:13.223621555" -======= - "timestamp": "2024-01-31T17:44:53.535994" ->>>>>>> dev } } \ No newline at end of file From f3a2da636d600241175ceb289eba75feb057cd59 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 27 Feb 2024 11:54:39 +0100 Subject: [PATCH 70/83] prepare 1.1.0 release --- CHANGELOG.md | 9 +++++++- assets/multiqc_config.yml | 4 ++-- modules.json | 23 ++----------------- modules/nf-core/gfaffix/main.nf | 4 ++-- .../nf-core/samtools/faidx/environment.yml | 4 ++-- modules/nf-core/samtools/faidx/main.nf | 4 ++-- nextflow.config | 2 +- 7 files changed, 19 insertions(+), 31 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 04673be8..5596a54e 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -2,7 +2,14 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 1.1.0dev - Schmuddlweddr +## 1.1.0 - Schmuddlweddr + +This release mostly contains updates of the respective modules. Functional updates in the following: + +- Added parameter `wfmash_hg_filter_ani_diff` +- `seqwish_min_match_length` new default now is 23. +- Fixed a bug with `wfmash_exclude_delim` where `#` was interpreted as a comment instead of a string. +- @heringerp added functionality so that very large integer values can be written in short form: For example 5000 would become 5k. ## 1.0.0 - Ratzupaltuff diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index e3d29f8d..8bd71204 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/pangenome + This report has been generated by the nf-core/pangenome analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-pangenome-methods-description": order: -1000 diff --git a/modules.json b/modules.json index 814a1538..82adcadf 100644 --- a/modules.json +++ b/modules.json @@ -7,7 +7,7 @@ "nf-core": { "gfaffix": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "c10d9fe2fb278db19840b150cc6b06d7e87960b5", "installed_by": ["modules"] }, "multiqc": { @@ -62,7 +62,7 @@ }, "samtools/faidx": { "branch": "master", - "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", + "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", "installed_by": ["modules"] }, "seqwish/induce": { @@ -87,25 +87,6 @@ } } }, - "subworkflows": { - "nf-core": { - "utils_nextflow_pipeline": { - "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", - "installed_by": ["subworkflows"] - }, - "utils_nfcore_pipeline": { - "branch": "master", - "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", - "installed_by": ["subworkflows"] - }, - "utils_nfvalidation_plugin": { - "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", - "installed_by": ["subworkflows"] - } - } - }, "subworkflows": { "nf-core": { "utils_nextflow_pipeline": { diff --git a/modules/nf-core/gfaffix/main.nf b/modules/nf-core/gfaffix/main.nf index 561e02d8..efbfe28f 100644 --- a/modules/nf-core/gfaffix/main.nf +++ b/modules/nf-core/gfaffix/main.nf @@ -5,8 +5,8 @@ process GFAFFIX { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gfaffix:0.1.5--h031d066_0 ' : - 'biocontainers/gfaffix:0.1.5--h031d066_0' }" + 'https://depot.galaxyproject.org/singularity/gfaffix:0.1.5b--h031d066_0' : + 'biocontainers/gfaffix:0.1.5b--h031d066_0' }" input: tuple val(meta), path(gfa) diff --git a/modules/nf-core/samtools/faidx/environment.yml b/modules/nf-core/samtools/faidx/environment.yml index 4807ba5f..3e95dd71 100644 --- a/modules/nf-core/samtools/faidx/environment.yml +++ b/modules/nf-core/samtools/faidx/environment.yml @@ -4,5 +4,5 @@ channels: - bioconda - defaults dependencies: - - bioconda::samtools=1.18 - - bioconda::htslib=1.18 + - bioconda::samtools=1.19.2 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf index d3461627..cfe7ad95 100644 --- a/modules/nf-core/samtools/faidx/main.nf +++ b/modules/nf-core/samtools/faidx/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_FAIDX { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : - 'biocontainers/samtools:1.18--h50ea8bc_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : + 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" input: tuple val(meta), path(fasta) diff --git a/nextflow.config b/nextflow.config index 37d322c8..f7a5ab74 100644 --- a/nextflow.config +++ b/nextflow.config @@ -276,7 +276,7 @@ manifest { description = """The pangenome graph construction pipeline renders a collection of sequences into a pangenome graph. Its goal is to build a graph that is locally directed and acyclic while preserving large-scale variation. Maintaining local linearity is important for interpretation, visualization, mapping, comparative genomics, and reuse of pangenome graphs""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.1.0dev' + version = '1.1.0' doi = '10.5281/zenodo.8202636' } From 0627dcd7a25a82e0aabb6142a429b06ed0f828e3 Mon Sep 17 00:00:00 2001 From: Simon Heumos Date: Tue, 27 Feb 2024 14:02:15 +0100 Subject: [PATCH 71/83] Update CHANGELOG.md MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 5596a54e..3ed11685 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -7,7 +7,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) This release mostly contains updates of the respective modules. Functional updates in the following: - Added parameter `wfmash_hg_filter_ani_diff` -- `seqwish_min_match_length` new default now is 23. +- `seqwish_min_match_length` new default value is `23` now. - Fixed a bug with `wfmash_exclude_delim` where `#` was interpreted as a comment instead of a string. - @heringerp added functionality so that very large integer values can be written in short form: For example 5000 would become 5k. From 3a99032f596e286b966d9a9a402f6cde9cd95937 Mon Sep 17 00:00:00 2001 From: Simon Heumos Date: Tue, 27 Feb 2024 14:02:39 +0100 Subject: [PATCH 72/83] Update CHANGELOG.md MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 3ed11685..a58fe11f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -4,7 +4,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) ## 1.1.0 - Schmuddlweddr -This release mostly contains updates of the respective modules. Functional updates in the following: +This release mostly contains updates of the respective modules. Functional updates are the following: - Added parameter `wfmash_hg_filter_ani_diff` - `seqwish_min_match_length` new default value is `23` now. From bc1f113b982d162aace74e122ea75d7b667a6a76 Mon Sep 17 00:00:00 2001 From: Simon Heumos Date: Tue, 27 Feb 2024 14:05:00 +0100 Subject: [PATCH 73/83] Update nextflow_schema.json MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 56eb9926..cf00963c 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -83,7 +83,7 @@ }, "wfmash_sparse_map": { "default": "1.0", - "description": "Keep this fraction of mappings (\"auto\" for giant component heuristic).", + "description": "Keep this fraction of mappings (`auto` for giant component heuristic).", "type": "string", "pattern": "(auto|[01]\\.\\d+)" }, From 6db3921ddfd8fb19ff031d494e97f5982ee14857 Mon Sep 17 00:00:00 2001 From: Simon Heumos Date: Tue, 27 Feb 2024 14:05:23 +0100 Subject: [PATCH 74/83] Update CHANGELOG.md MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index a58fe11f..ed43cb56 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -9,7 +9,7 @@ This release mostly contains updates of the respective modules. Functional updat - Added parameter `wfmash_hg_filter_ani_diff` - `seqwish_min_match_length` new default value is `23` now. - Fixed a bug with `wfmash_exclude_delim` where `#` was interpreted as a comment instead of a string. -- @heringerp added functionality so that very large integer values can be written in short form: For example 5000 would become 5k. +- @heringerp added functionality so that very large integer values can be written in short form: For example `5000` would become `5k`. ## 1.0.0 - Ratzupaltuff From 2a659eef6e54b23bc66054f3b7c27e0bf2efb791 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 27 Feb 2024 14:10:31 +0100 Subject: [PATCH 75/83] more detailed wfmash_hg_filter_ani_diff explanation --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ed43cb56..fac25536 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -6,7 +6,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) This release mostly contains updates of the respective modules. Functional updates are the following: -- Added parameter `wfmash_hg_filter_ani_diff` +- Added parameter `wfmash_hg_filter_ani_diff` mirroring https://github.com/pangenome/pggb/pull/351: Filter out mappings unlikely to be this Average Nuclotide Identitiy (ANI) less than the best mapping. The default values is `30`. - `seqwish_min_match_length` new default value is `23` now. - Fixed a bug with `wfmash_exclude_delim` where `#` was interpreted as a comment instead of a string. - @heringerp added functionality so that very large integer values can be written in short form: For example `5000` would become `5k`. From e91f751b0bae61237e8fef58bb5abc48a80b9e55 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 27 Feb 2024 14:18:49 +0100 Subject: [PATCH 76/83] description for wfmash_hg_filter_ani_diff --- nextflow_schema.json | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index cf00963c..d042da1d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -117,7 +117,8 @@ }, "wfmash_hg_filter_ani_diff": { "type": "integer", - "default": 30 + "default": 30, + "description": "Filter out mappings unlikely to be this Average Nucleotide Identity (ANI) less than the best mapping." } } }, From b986c4a7d9215c575cb80d522b11d6b64d010486 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 27 Feb 2024 14:34:37 +0100 Subject: [PATCH 77/83] delete assets/schema_input.json --- assets/schema_input.json | 33 --------------------------------- 1 file changed, 33 deletions(-) delete mode 100644 assets/schema_input.json diff --git a/assets/schema_input.json b/assets/schema_input.json deleted file mode 100644 index 596a8b62..00000000 --- a/assets/schema_input.json +++ /dev/null @@ -1,33 +0,0 @@ -{ - "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/pangenome/master/assets/schema_input.json", - "title": "nf-core/pangenome pipeline - params.input schema", - "description": "Schema for the file provided with params.input", - "type": "array", - "items": { - "type": "object", - "properties": { - "sample": { - "type": "string", - "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces", - "meta": ["id"] - }, - "fastq_1": { - "type": "string", - "format": "file-path", - "exists": true, - "pattern": "^\\S+\\.f(ast)?q\\.gz$", - "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" - }, - "fastq_2": { - "type": "string", - "format": "file-path", - "exists": true, - "pattern": "^\\S+\\.f(ast)?q\\.gz$", - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" - } - }, - "required": ["sample", "fastq_1"] - } -} From 8d35f156cb85288891b53787ac2c4f846d46abf7 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 27 Feb 2024 14:57:04 +0100 Subject: [PATCH 78/83] update toolCitationText() --- CITATIONS.md | 2 +- .../utils_nfcore_pangenome_pipeline/main.nf | 19 +++++++++++++++++-- 2 files changed, 18 insertions(+), 3 deletions(-) diff --git a/CITATIONS.md b/CITATIONS.md index 82f86f42..9e503d03 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -58,7 +58,7 @@ - [WFMASH](https://github.com/waveygang/wfmash) - > Guarracino A, Mwaniki N, Marco-Sola S, Garrison E. Wfmash: whole-chromosome pairwise alignment using the hierarchical wavefront algorithm. 2023. https://github.com/waveygang/wfmash. + > Guarracino A, Mwaniki N, Marco-Sola S, Garrison E. Wfmash: whole-chromosome pairwise alignment using the hierarchical wavefront algorithm. 2024. https://github.com/waveygang/wfmash. ## Software packaging/containerisation tools diff --git a/subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf b/subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf index 9fc5022f..4760e0e5 100644 --- a/subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf @@ -186,8 +186,24 @@ def toolCitationText() { // Uncomment function in methodsDescriptionText to render in MultiQC report def citation_text = [ "Tools used in the workflow included:", - "FastQC (Andrews 2010),", + "NF-CORE (Ewels et al. 2020)", + "Nextflow (Di Tommaso et al. 2017)", + "GFAFFIX (Liao et al. 2023)", "MultiQC (Ewels et al. 2016)", + "NET2COMMUNITIES (Traag et al. 2019)", + "ODGI (Guarracino, Heumos et al. 2022; Heumos, Guarracino et al. 2023)", + "PGGB (Garrison, Guarracino et al. 2023; Guarracino et al. 2023; Liao et al. 2023)", + "SAMTOOLS (Li et al. 2009)", + "SEQWISH (Garrison and Guarracino 2023)", + "SMOOTHXG (Garrison, Guarracino et al. 2023)", + "VCFLIB (Garrison et al. 2022)", + "VG (Garrison et al. 2018)", + "WFMASH (Guarracino et al. 2024)", + "Anaconda (Anaconda Software Distribution 2016)", + "Bioconda (Grüning et al. 2018)", + "BioContainers (da Veiga Leprevost et al. 2017)", + "Docker (Merkel 2014)", + "Singularity (Kurtzer et al. 2017)", "." ].join(' ').trim() @@ -199,7 +215,6 @@ def toolBibliographyText() { // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", // Uncomment function in methodsDescriptionText to render in MultiQC report def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " ].join(' ').trim() From 4fafe05f15ce2bec5d1d4f48f0891fda2757eb9b Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 27 Feb 2024 15:00:04 +0100 Subject: [PATCH 79/83] better help text for wfmash_exclude_delim --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index d042da1d..568c8c47 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -98,7 +98,7 @@ }, "wfmash_exclude_delim": { "type": "string", - "description": "Skip mappings between sequences with the same name prefix before the given delimiter character. [default: all-vs-all and !self]." + "description": "Skip mappings between sequences with the same name prefix before the given delimiter character. This can be helpful if several sequences originate from the same chromosome. It is recommended that the sequence names respect the https://github.com/pangenome/PanSN-spec." }, "wfmash_temp_dir": { "type": "string", From 2876894c767d2a1c09c3f34f746a0757bfe3362d Mon Sep 17 00:00:00 2001 From: subwaystation Date: Tue, 27 Feb 2024 15:09:15 +0100 Subject: [PATCH 80/83] update toolBibliographyText() --- CITATIONS.md | 2 +- .../utils_nfcore_pangenome_pipeline/main.nf | 20 ++++++++++++++++++- 2 files changed, 20 insertions(+), 2 deletions(-) diff --git a/CITATIONS.md b/CITATIONS.md index 9e503d03..209db82b 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -54,7 +54,7 @@ - [VG](https://pubmed.ncbi.nlm.nih.gov/30125266/) - > Garrison E et al. Variation graph toolkit improves read mapping by representing genetic variation in the reference. Nature biotechnology vol. 36,9 (2018): 875-879. doi: https://10.1038/nbt.4227 + > Garrison E et al. Variation graph toolkit improves read mapping by representing genetic variation in the reference. Nature biotechnology vol. 36,9 (2018): 875-879. doi: https://10.1038/nbt.4227. - [WFMASH](https://github.com/waveygang/wfmash) diff --git a/subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf b/subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf index 4760e0e5..994c1234 100644 --- a/subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_pangenome_pipeline/main.nf @@ -215,7 +215,25 @@ def toolBibliographyText() { // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", // Uncomment function in methodsDescriptionText to render in MultiQC report def reference_text = [ - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + "
  • Ewels PA, Peltzer A, Fillinger S, Patel H, Alneberg J, Wilm A, Garcia MU, Di Tommaso P, Nahnsen S. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020 Mar;38(3):276-278. doi: https://10.1038/s41587-020-0439-x. PubMed PMID: 32055031.
    • ", + "
  • Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: https://10.1038/nbt.3820. PubMed PMID: 28398311.
    • ", + "
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    • ", + "
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    • " ].join(' ').trim() return reference_text From 203c65face5997364c235115a0fdf379749150f5 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Wed, 28 Feb 2024 10:05:01 +0100 Subject: [PATCH 81/83] proper doi for the pipeline --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index f7a5ab74..5acdc2e7 100644 --- a/nextflow.config +++ b/nextflow.config @@ -277,7 +277,7 @@ manifest { mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' version = '1.1.0' - doi = '10.5281/zenodo.8202636' + doi = 'https://doi.org/10.5281/zenodo.8202636' } // Load modules.config for DSL2 module specific options From 02725ae5b26dea688e551c0b0a2d7b771a7e437d Mon Sep 17 00:00:00 2001 From: subwaystation Date: Wed, 28 Feb 2024 10:08:29 +0100 Subject: [PATCH 82/83] fix typos --- subworkflows/local/odgi_qc.nf | 2 +- subworkflows/local/pggb.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/odgi_qc.nf b/subworkflows/local/odgi_qc.nf index 48720552..afdce1e3 100644 --- a/subworkflows/local/odgi_qc.nf +++ b/subworkflows/local/odgi_qc.nf @@ -18,7 +18,7 @@ workflow ODGI_QC { seqwish // file: /path/to/seqwish.og sorted_graph // file: /path/to/sorted_graph.og community_mode // val : determines how to handly meta identifiers - no_seqwish_input // val : determiens how we will sort the input for MULTIQC + no_seqwish_input // val : determines how we will sort the input for MULTIQC main: diff --git a/subworkflows/local/pggb.nf b/subworkflows/local/pggb.nf index 20991fea..d70b8680 100644 --- a/subworkflows/local/pggb.nf +++ b/subworkflows/local/pggb.nf @@ -22,7 +22,7 @@ workflow PGGB { fai // file: /path/to/sequences.fasta.fai gzi // file: /path/to/sequences.fasta.gzi community_mode // val: determines how we will build our meta identifiers of ODGI_QC - no_seqwish_input // val : determiens how we will sort the input for MULTIQC + no_seqwish_input // val : determines how we will sort the input for MULTIQC main: From d51fa7a7e1137bc72b3f5fff082e3237990610f5 Mon Sep 17 00:00:00 2001 From: subwaystation Date: Wed, 28 Feb 2024 10:14:23 +0100 Subject: [PATCH 83/83] tell users that in the future the PanSN spec will be mandatory --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 568c8c47..2b6a4a07 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -98,7 +98,7 @@ }, "wfmash_exclude_delim": { "type": "string", - "description": "Skip mappings between sequences with the same name prefix before the given delimiter character. This can be helpful if several sequences originate from the same chromosome. It is recommended that the sequence names respect the https://github.com/pangenome/PanSN-spec." + "description": "Skip mappings between sequences with the same name prefix before the given delimiter character. This can be helpful if several sequences originate from the same chromosome. It is recommended that the sequence names respect the https://github.com/pangenome/PanSN-spec. In future versions of the pipeline it will be required that the sequence names follow this specification." }, "wfmash_temp_dir": { "type": "string",
    Process Name \\", - " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls