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Installation instructions for levioSAM2

levioSAM2 supports a variety of methods for installation:

  • Conda
  • Docker
  • Singularity
  • CMake

Install levioSAM2 via Conda

conda install -c conda-forge -c bioconda leviosam2

Use a levioSAM2 Docker image

You can obtain a Docker image of the latest version from Docker hub:

docker pull naechyun/leviosam2:latest

Use a levioSAM2 Singularity image

singularity pull docker://naechyun/leviosam2:latest

Intall levioSAM2 from scratch using CMake

Dependencies

Make sure the following prerequisite libraries are installed on your system.

The dependent libraries can be installed through the following package manager options, or built from scratch:

# Conda
conda install -c conda-forge sdsl-lite
conda install -c bioconda htslib

# Debian/Ubuntu
apt-get install libhts-dev libsdsl-dev 

# MacOS
brew tap brewsci/bio
brew install htslib sdsl-lite

# RedHat or Fedora
yum install htslib
# sdsl-lite needs to be installed manually

CMake

Command:

mkdir build
cd build
cmake ..
make
# make install
# or 
# make install DESTDIR=/path/to/install

If you installed the dependencies manually, you might need to provide the path of the dependent libraries to cmake by using the following command:

cmake -D CMAKE_LIBRARY_PATH="/path/to/libsdsl/;/path/to/libhts/" \
      -D CMAKE_INCLUDE_PATH="/path/to/libsdsl/include/;/path/to/libhts/include/" ..

Testing

We provide an end-to-end test and a set of unit tests for levioSAM.

  • The end-to-end test can be run with python leviosam-test.py. This test includes running levioSAM on several test files in testdata. We also use picard to validate the lifted results.

  • The unit test can be run with the command cd build; ctest --verbose.

ARM64

LevioSAM2 supports the arm64 architecture. Note that the distributed libraries of the dependencies (namely htslib and sdsl-lite) on Conda or other package managers might not support arm64. Thus, you might need to build the dependent libraries from source. Both htslib and sdsl-lite can be built under the arm64 architecture.

Notes:

  1. sdsl-lite==v2.1.1 also installs gtest and this can result in errors when building levioSAM2 due to duplicated declarations. A work-around is to remove the gtest headers installed along with sdsl-lite.

  2. There can be dynamic library linking errors when executing levioSAM2. This can be solved by export DYLD_LIBRARY_PATH=/path/to/libsdsl/:/path/to/libhts/:$DYLD_LIBRARY_PATH.