diff --git a/Src/Common/W3C/W3CConstants.cs b/Src/Common/W3C/W3CConstants.cs
index 1688e2783..580855654 100644
--- a/Src/Common/W3C/W3CConstants.cs
+++ b/Src/Common/W3C/W3CConstants.cs
@@ -63,7 +63,7 @@ public static class W3CConstants
         /// <summary>
         /// Default sampled flag value.
         /// </summary>
-        internal const string DefaultSampled = "01";
+        internal const string DefaultSampled = "00";
 
         /// <summary>
         /// Name of the environment variable that controls W3C distributed tracing support.
diff --git a/Src/DependencyCollector/Net45.Tests/DependencyTrackingTelemetryModuleHttpTest.cs b/Src/DependencyCollector/Net45.Tests/DependencyTrackingTelemetryModuleHttpTest.cs
index 1f80abbc3..82e0decf7 100644
--- a/Src/DependencyCollector/Net45.Tests/DependencyTrackingTelemetryModuleHttpTest.cs
+++ b/Src/DependencyCollector/Net45.Tests/DependencyTrackingTelemetryModuleHttpTest.cs
@@ -353,7 +353,7 @@ public void TestDependencyCollectionWithW3CHeadersDiagnosticSource()
                 Assert.AreEqual(expectedTraceId, dependency.Context.Operation.Id);
                 Assert.AreEqual(expectedParentId, dependency.Context.Operation.ParentId);
 
-                Assert.AreEqual($"00-{expectedTraceId}-{dependency.Id}-01", request.Headers[W3CConstants.TraceParentHeader]);
+                Assert.AreEqual($"00-{expectedTraceId}-{dependency.Id}-00", request.Headers[W3CConstants.TraceParentHeader]);
 
                 Assert.AreEqual($"{W3CConstants.ApplicationIdTraceStateField}={expectedAppId},state=some", request.Headers[W3CConstants.TraceStateHeader]);
 
diff --git a/Src/DependencyCollector/NetCore.Tests/DependencyTrackingTelemetryModuleTestNetCore.cs b/Src/DependencyCollector/NetCore.Tests/DependencyTrackingTelemetryModuleTestNetCore.cs
index d1a6bd53c..0e6811789 100644
--- a/Src/DependencyCollector/NetCore.Tests/DependencyTrackingTelemetryModuleTestNetCore.cs
+++ b/Src/DependencyCollector/NetCore.Tests/DependencyTrackingTelemetryModuleTestNetCore.cs
@@ -246,7 +246,7 @@ public async Task TestDependencyCollectionWithW3CHeadersAndRequestId()
                 Assert.AreEqual(expectedParentId, dependency.Context.Operation.ParentId);
 
                 Assert.IsTrue(request.Headers.Contains(W3CConstants.TraceParentHeader));
-                Assert.AreEqual($"00-{expectedTraceId}-{dependency.Id}-01", request.Headers.GetValues(W3CConstants.TraceParentHeader).Single());
+                Assert.AreEqual($"00-{expectedTraceId}-{dependency.Id}-00", request.Headers.GetValues(W3CConstants.TraceParentHeader).Single());
 
                 Assert.IsTrue(request.Headers.Contains(W3CConstants.TraceStateHeader));
                 Assert.AreEqual($"{W3CConstants.ApplicationIdTraceStateField}={expectedAppId},state=some", request.Headers.GetValues(W3CConstants.TraceStateHeader).Single());
@@ -296,7 +296,7 @@ public async Task TestDependencyCollectionWithW3CHeadersAndNoParentContext()
                 Assert.AreEqual(expectedParentId, dependency.Context.Operation.ParentId);
 
                 Assert.IsTrue(request.Headers.Contains(W3CConstants.TraceParentHeader));
-                Assert.AreEqual($"00-{expectedTraceId}-{dependency.Id}-01", request.Headers.GetValues(W3CConstants.TraceParentHeader).Single());
+                Assert.AreEqual($"00-{expectedTraceId}-{dependency.Id}-00", request.Headers.GetValues(W3CConstants.TraceParentHeader).Single());
 
                 Assert.IsTrue(request.Headers.Contains(W3CConstants.TraceStateHeader));
                 Assert.AreEqual($"{W3CConstants.ApplicationIdTraceStateField}={expectedAppId}", request.Headers.GetValues(W3CConstants.TraceStateHeader).Single());