diff --git a/CHANGES.txt b/CHANGES.txt
index a88445e9b..24778c956 100644
--- a/CHANGES.txt
+++ b/CHANGES.txt
@@ -14,6 +14,7 @@ https://mhammond.github.io/pywin32_installers.html.
 Coming in build 309, as yet unreleased
 --------------------------------------
 
+* Fixed `nbios.NCBStruct` packing (#2406, @Avasam)
 * Fail sooner on invalid `win32timezone.TimeZoneInfo` creation (#2338, @Avasam)
 * Removed temporary `win32com.server.policy` reexports hack (#2344, @Avasam)
   Import `DispatcherWin32trace` and `DispatcherTrace` from `win32com.server.dispatcher` instead.
diff --git a/win32/Lib/netbios.py b/win32/Lib/netbios.py
index 3da1291c9..7503db642 100644
--- a/win32/Lib/netbios.py
+++ b/win32/Lib/netbios.py
@@ -1,4 +1,7 @@
+from __future__ import annotations
+
 import struct
+from collections.abc import Iterable
 
 import win32wnet
 
@@ -191,7 +194,7 @@ def Netbios(ncb):
 
 
 class NCBStruct:
-    def __init__(self, items):
+    def __init__(self, items: Iterable[tuple[str, str]]) -> None:
         self._format = "".join([item[0] for item in items])
         self._items = items
         self._buffer_ = win32wnet.NCBBuffer(struct.calcsize(self._format))
@@ -199,20 +202,18 @@ def __init__(self, items):
         for format, name in self._items:
             if len(format) == 1:
                 if format == "c":
-                    val = "\0"
+                    val: bytes | int = b"\0"
                 else:
                     val = 0
             else:
                 l = int(format[:-1])
-                val = "\0" * l
+                val = b"\0" * l
             self.__dict__[name] = val
 
     def _pack(self):
-        vals = []
-        for format, name in self._items:
-            vals.append(self.__dict__.get(name))
+        vals = [self.__dict__.get(name) for format, name in self._items]
 
-        self._buffer_[:] = struct.pack(*(self._format,) + tuple(vals))
+        self._buffer_[:] = struct.pack(self._format, *vals)
 
     def _unpack(self):
         items = struct.unpack(self._format, self._buffer_)