diff --git a/testing/buildbot/gn_isolate_map.pyl b/testing/buildbot/gn_isolate_map.pyl
index 6da7d05103965f..6176156d673eeb 100644
--- a/testing/buildbot/gn_isolate_map.pyl
+++ b/testing/buildbot/gn_isolate_map.pyl
@@ -1767,7 +1767,6 @@
     "label": "//chrome/test:telemetry_unittests",
     "script": "//testing/scripts/run_telemetry_as_googletest.py",
     "type": "script",
-    "python3": True,
   },
   "telemetry_weblayer_apks": {
     "label": "//tools/perf/chrome_telemetry_build:telemetry_weblayer_apks",