diff --git a/MAESTRO/Snakemake/multi_scATAC/rules/sc_atac_peak_count_batch.smk b/MAESTRO/Snakemake/multi_scATAC/rules/sc_atac_peak_count_batch.smk index 3195ea58..92ef792e 100644 --- a/MAESTRO/Snakemake/multi_scATAC/rules/sc_atac_peak_count_batch.smk +++ b/MAESTRO/Snakemake/multi_scATAC/rules/sc_atac_peak_count_batch.smk @@ -38,7 +38,7 @@ rule scatac_countpeak_batch: input: finalpeak = "Result/Analysis/Batch/all_samples_peaks.bed", validbarcode = "Result/QC/{sample}/{sample}_scATAC_validcells.txt", - frag = get_fragments + frag = "Result/minimap2/{sample}/fragments_corrected_dedup_count.tsv" output: counts = "Result/Analysis/Batch/{sample}/{sample}_peak_count.h5" params: diff --git a/README.md b/README.md index 041dc3dc..a12c1f85 100644 --- a/README.md +++ b/README.md @@ -88,7 +88,7 @@ $ conda config --add channels bioconda $ conda config --add channels conda-forge # To make the installation faster, we recommend using mamba $ conda install mamba -c conda-forge -$ mamba create -n MAESTRO maestro=1.3.1 -c liulab-dfci +$ mamba create -n MAESTRO maestro=1.3.2 -c liulab-dfci # Activate the environment $ conda activate MAESTRO ``` diff --git a/conda/MAESTRO/meta.yaml b/conda/MAESTRO/meta.yaml index 37902613..58f5ae15 100644 --- a/conda/MAESTRO/meta.yaml +++ b/conda/MAESTRO/meta.yaml @@ -19,12 +19,12 @@ requirements: - snakemake >=5.14 - rseqc >=3.0.0 - star =2.7.6a - - minimap2 >=2.17 - - samtools >=1.9 - - bedtools >=2.29.2 + - minimap2 =2.17 + - samtools =1.10 + - bedtools =2.29.2 - rsem >=1.3.2 - picard >=2.22.0 - - macs2 >=2.2.6 + - macs2 =2.2.7.1 - lisa2 =2.2.2 - umap-learn - pysam # this is needed by sinto @@ -86,13 +86,13 @@ requirements: - snakemake >=5.14 - rseqc >=3.0.0 - star =2.7.6a - - minimap2 >=2.17 - - samtools >=1.9 - - bedtools >=2.29.2 + - minimap2 =2.17 + - samtools =1.10 + - bedtools =2.29.2 - rsem >=1.3.2 - picard >=2.22.0 - - macs2 >=2.2.6 - - lisa2 >=2.1.0 + - macs2 =2.2.7.1 + - lisa2 =2.2.2 - umap-learn - pysam # this is needed by sinto - r-base >=4.0.2