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NEWS
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CHANGES IN VERSION 1.5.2
-----------------------
SIGNIFICANT USER-VISIBLE CHANGES
o assignSpecies has been reimplemented to be much, much faster (>100x faster with enough query sequences).
o The allowOneOff flag has been set to FALSE by default in the xxxBimeraDenovo functions.
BUG FIXES
o The compress option is now respected by trimAndFilter.
CHANGES IN VERSION 1.5.1
-----------------------
SIGNIFICANT USER-VISIBLE CHANGES
o The parsing functions to create the training fastas compatible with assignTaxonomy and assignSpecies from the Silva and RDP databases are now included as private functions in the taxonomy.R file. In parallel, updated versions of both RDP (trainset 16) and Silva (v128) training fastas were released on Zenodo.
CHANGES IN VERSION 1.3.5
-----------------------
SIGNIFICANT USER-VISIBLE CHANGES
o The default chimera removal method is now "consensus" in the removeBimeraDenovo function (method="consensus").
o The filtering functions now remove phiX by default (rm.phix=TRUE).
CHANGES IN VERSION 1.3.4
-----------------------
NEW FEATURES
o filterAndTrim is the new multithreaded interface for filtering and trimming. It is R-vectorized, in that it accepts vectors of input/output files. Supports both single-read and paired-read filtering.
SIGNIFICANT USER-VISIBLE CHANGES
o Consensus chimera removal now supports multithreading.
o The new trimOverhang option in mergePairs allows the portion of paired reads that overhang the start of the other read to be removed when merging.
o Faster filtering by not enforcing redundant filtering conditions.
CHANGES IN VERSION 1.3.3
-----------------------
NEW FEATURES
o ITS support. The dada method now handles variable-length amplicons, which allows common ITS sequencing strategies to be processed. Taxonomic assignment against the UNITE database is also now available.
SIGNIFICANT USER-VISIBLE CHANGES
o The xxxFilter methods now have maxLen and minLen filtering parameters.
CHANGES IN VERSION 1.3.2
-----------------------
NEW FEATURES
o learnErrors is a new method for learning error rates from a targeted number of reads. This wraps pre-existing functionality -- dada(..., selfConsist=TRUE) -- in a much easier to use form.
o assignTaxonomy is multithreaded (multithread=TRUE) and can individually test reverse-complemented sequences and use them to classify if they better match the reference (tryRC=TRUE).
CHANGES IN VERSION 1.3.1
-----------------------
SIGNIFICANT USER-VISIBLE CHANGES
o The xxxFilter methods now (invisibily) return the number of reads that went in and that passed the filter.
o fastqFilter now has a maxLen parameter, primarily for filtering/trimming 454 data.
BUG FIXES
o trimLeft is now enforced before any filtering is performed.
o Rscript invocations should now work without requiring an initial library(methods) call.
CHANGES IN VERSION 1.1.7
-----------------------
SIGNIFICANT USER-VISIBLE CHANGES
o The isBimeraDenovoTable method is now available through the convenience interface removeBimeraDenovo(..., tableMethod="consensus").
o The vectorized Needleman-Wunsch algorithm is now available through nwalign(..., vec=TRUE). This aligner is very fast for short sequences, but performs no overflow checking so use on longer sequences (2kb+) with care.
BUG FIXES
o It is now more difficult to mess up the internal structure of dada-class and derep-class objects.
CHANGES IN VERSION 1.1.6
-----------------------
NEW FEATURES
o isBimeraDenovoTable is a new function for detecting chimeras on multi-sample sequence tables. It first identifies bimeras on a per-sample basis, and then uses a consensus approach to identify bimeric sequences. This improves the specificity of bimera classification for datasets with many samples.
SIGNIFICANT USER-VISIBLE CHANGES
o The filenames is now included in the plotQualityProfile plot.
BUG FIXES
o assignTaxonomy now works for sequences containg Ns.
o Bimera detection now correctly handles non-unique sequences in the input.
o Fixed a memory leak when using the HOMOPOLYMER_GAP_PENALTY option.
o Warnings and errors during error estimation from small samples in the dada(…) function are now handled appropriately.
CHANGES IN VERSION 1.1.4
-----------------------
NEW FEATURES
o Multithreaded chimera detection. The isBimeraDenovo(...) function is now multithreaded. This behavior is controlled by the multithread argument to the function, and is FALSE by default.
CHANGES IN VERSION 1.1.3
-----------------------
SIGNIFICANT USER-VISIBLE CHANGES
o The vectorized aligner has been updated. It is now more flexible, handling different length sequences and variable end-gap penalties. It is also faster: On default settings the dada(...) function is now 15-25% faster, and isBimeraDenovo is 2-4x faster.
CHANGES IN VERSION 1.1.2
-----------------------
NEW FEATURES
o Species-level taxonomic assignment. The new assignSpecies(...) function uses exact matching to assign sequences to the species level. Currently a valid species training fasta is only available for the RDP taxonomic database.
CHANGES IN VERSION 1.1.1
-----------------------
BUG FIXES
o Fixed the memory leak in isBimeraDenovo and friends.
o Sequence tables are now integer rather than numeric.
CHANGES IN VERSION 1.1
-----------------------
NEW FEATURES
o Multithreading. The dada(...) function is now multithreaded. This behavior is controlled by the multithread argument to the function, and is FALSE by default.
SIGNIFICANT USER-VISIBLE CHANGES
o The phiX removal functionality in fastqFilter/fastqPairedFilter is now substantially faster.
BUG FIXES
o Certain diagnostic return values of dada(..., pool=TRUE) are now appropriately constructed.
o A 2-5% speedup from switching to C++11 hashed containers.
CHANGES IN VERSION 1.0
-----------------------
NEW FEATURES
o The dada2 package is now available from Bioconductor (http://bioconductor.org)
CHANGES IN VERSION 0.99.9
-----------------------
NEW FEATURES
o fastqFilter and fastqPairedFilter can now remove phiX contamination if rm.phix argument set to TRUE
CHANGES IN VERSION 0.99.8
-----------------------
SIGNIFICANT USER-VISIBLE CHANGES
o Banding in nwalign is now turned off by default
BUG FIXES
o Graceful handling of sequences which are not classified at any level by assignTaxonomy
CHANGES IN VERSION 0.99.7
-----------------------
SIGNIFICANT USER-VISIBLE CHANGES
o isBimera was rewritten in C, significantly increasing speed
BUG FIXES
o An edge case bimera detection bug was fixed
CHANGES IN VERSION 0.99.6
-----------------------
SIGNIFICANT USER-VISIBLE CHANGES
o Function documentation was reviewed and revised throughout the package
CHANGES IN VERSION 0.99.5
-----------------------
NEW FEATURES
o plotQualityProfile displays a visual summary of the quality scores over sequences in a fastq file
o removeBimeraDenovo conveniently identifies and removes chimeras from the input unique sequences
SIGNIFICANT USER-VISIBLE CHANGES
o The dada2 package is now part of the devel branch of Bioconductor (http://bioconductor.org)
BUG FIXES
o assignTaxonomy now handles varying levels of taxonomic classification in the training data
CHANGES IN VERSION 0.10.7
-----------------------
NEW FEATURES
o dada2 now supports 454 pyrosequencing. When calling the dada function on 454 data, we recommend
using the parameters USE_QUALS = FALSE, HOMOPOLYMER_GAP_PENALTY = -1, BAND_SIZE = 32
SIGNIFICANT USER-VISIBLE CHANGES
o mergePairs is now "vectorized" over lists of input dada-class and derep-class objects
o Added a homo_gap argument to nwalign which sets the homopolymer gap penalty in the N-W alignment
BUG FIXES
o assignTaxonomy now uses less memory