diff --git a/lotus2 b/lotus2 index cf26c7a..4f5658b 100755 --- a/lotus2 +++ b/lotus2 @@ -3331,7 +3331,7 @@ my %workflow_options = ( my $taxonomy_heading = "Taxonomy Options"; my %taxonomy_options = ( - '-refDB ', '(SLV) Silva LSU (23/28S) or SSU (16/18S), (GG greengenes (only SSU available), (HITdb) (SSU, human gut specific), (PR2) LSU spezialized on Ocean environmentas, (UNITE) ITS fungi specific, (beetax) bee gut specific database and tax names. Given that \"-amplicon_type \" was set to SSU or LSU, the appropriate DB in SLV would be used. \nDecide which reference DB will be used for a similarity based taxonomy annotation. Databases can be combined, with the first having the highest priority. E.g. "HITdb,SLV" would priority assign OTUs to PR2 taxonomy, but hits with a higher %id to SLV would be assigned to SLV. Can also be a custom fasta formatted database: in this case provide the path to the fasta file as well as the path to the taxonomy for the sequences using -tax4refDB. For custom databases QIIME2 file formats are compatible if the delimiter in the QIIME2 taxonomy file is changed from semicolon to tab. See also online help on how to create a custom DB. WARNING: combining databases with incompatible tax levels (e.g. PR2,SLV) will result in non sensical tax levels. (Default: SLV)', + '-refDB ', '(SLV) Silva LSU (23/28S) or SSU (16/18S), (GG2) GreenGenes2 (only SSU available), (HITdb) (SSU, human gut specific), (PR2) LSU spezialized on Ocean environmentas, (UNITE) ITS fungi specific, (beetax) bee gut specific database and tax names. Given that \"-amplicon_type \" was set to SSU or LSU, the appropriate DB in SLV would be used. \nDecide which reference DB will be used for a similarity based taxonomy annotation. Databases can be combined, with the first having the highest priority. E.g. "HITdb,SLV" would priority assign OTUs to PR2 taxonomy, but hits with a higher %id to SLV would be assigned to SLV. Can also be a custom fasta formatted database: in this case provide the path to the fasta file as well as the path to the taxonomy for the sequences using -tax4refDB. For custom databases QIIME2 file formats are compatible if the delimiter in the QIIME2 taxonomy file is changed from semicolon to tab. See also online help on how to create a custom DB. WARNING: combining databases with incompatible tax levels (e.g. PR2,SLV) will result in non sensical tax levels. (Default: SLV)', '-tax4refDB ', 'In conjunction with a custom fasta file provided to argument -refDB, this file contains for each fasta entry in the reference DB a taxonomic annotation string, with the same number of taxonomic levels for each, tab separated.', '-amplicon_type ', '(SSU) small subunit (16S/18S), (LSU) large subunit (23S/28S) or internal transcribed spacer (ITS|ITS1|ITS2), (custom) for custom marker genes. These options will change default read qual filter parameters and activate ITS specific postfiltering steps. (Default: SSU)', '-tax_group ', '(bacteria) bacterial 16S rDNA annnotation, (fungi) fungal 18S/23S/ITS annotation, (eukarya) eukaryotic (18S/23S) annotation. (Default: bacteria)',