SEGUL

SEGUL simplifies complex, tedious, and error-prone data wrangling and summarization for genomics and Sanger datasets. We develop it to be easy for beginners while providing advanced features for experienced users. SEGUL is also a high performance and memory efficient genomic tool. In our tests, it consistently offers a faster and more efficient (low memory footprint) alternative to existing applications for various genomic tasks (see benchmark).

SEGUL runs on many software platforms, from mobile devices and personal computers to high-performance computing clusters. It is available as a command-line interface (CLI), a graphical user interface (GUI) application, and a Rust library and Python package (see platform support below). SEGUL is part of our ongoing effort to ensure that genomic software is accessible to everyone, regardless of their bioinformatic skills and computing resources.

Learn more about SEGUL in the documentation. We welcome feedback if you find any issues, difficulties or have ideas to improve the app and its documentation (details below).

Citation

Handika, H., and J. A. Esselstyn. 2024. SEGUL: Ultrafast, memory-efficient and mobile-friendly software for manipulating and summarizing phylogenomic datasets. Molecular Ecology Resources. https://doi.org/10.1111/1755-0998.13964.

Links

• App Documentation: [EN]
• API Documentation: [Rust]
• GUI: [Source code]
• Python API: [Source code] [PyPI]

Supported File Formats

Sequence formats:

1. NEXUS
2. Relaxed PHYLIP
3. FASTA
4. FASTQ (gzipped and uncompressed)
5. Multiple Alignment Format (MAF) (In development)
6. Variant Call Format (VCF) (In development)

All formats are supported in interleave and sequential versions. The app supports DNA and amino acid sequences, except for FASTQ, MAF, and VCF (DNA only).

Alignment partition formats:

1. RaXML
2. NEXUS

The NEXUS partition can be written as a charset block embedded in NEXUS formatted sequences or a separate file.

Installation

GUI Version

Desktop

Mobile

Learn more about device requirements and GUI app installation in the documentation.

CLI Version

The CLI app may work in any Rust-supported platform. However, we only tested and officially support the following platforms:

• Linux
• MacOS
• Windows
• Windows Subsystem for Linux (WSL)

CLI Installation Methods

• Pre-compiled binaries: [Releases] [Docs]
• Conda: [Package] [Docs]
• Package manager: [Docs]
• From source: [Docs]
• Beta version: [Docs]

API version

The API version is available for Rust and other programming languages. For Rust users, you can install it via Cargo:

cargo add segul

Python

We provide binding for Python (called pysegul). Use SEGUL just like any other Python package:

pip install pysegul

Learn more about using SEGUL API in the documentation.

Features

NOTES: To try beta features, follow the installation instruction for the beta version.

Features	Supported Input Formats	Guideline Quick Links
Alignment concatenation	FASTA, NEXUS, PHYLIP	CLI / GUI / Python
Alignment conversion	FASTA, NEXUS, PHYLIP	CLI / GUI / Python
Alignment filtering	FASTA, NEXUS, PHYLIP	CLI / GUI / Python
Alignment splitting	FASTA, NEXUS, PHYLIP	CLI / GUI / Python
Alignment partition conversion	RaXML, NEXUS	CLI / GUI / Python
Alignment summary statistics	FASTA, NEXUS, PHYLIP	CLI / GUI / Python
Alignment trimming	FASTA, NEXUS, PHYLIP	CLI (Beta) / Coming soon
Genomic summary statistics	FASTQ, FASTA (contigs)	CLI / GUI / Python
Multiple alignment conversion	MAF	CLI (Beta) / Coming soon
Sequence addition	FASTA, NEXUS, PHYLIP	CLI (Beta) / Coming soon
Sequence extraction	FASTA, NEXUS, PHYLIP	CLI / GUI / Python
Sequence filtering	FASTA, NEXUS, PHYLIP	CLI / Coming soon
Sequence ID extraction	FASTA, NEXUS, PHYLIP	CLI / GUI / Python
Sequence ID mapping	FASTA, NEXUS, PHYLIP	CLI / GUI / Python
Sequence ID renaming	FASTA, NEXUS, PHYLIP	CLI / GUI / Coming soon
Sequence removal	FASTA, NEXUS, PHYLIP	CLI / GUI / Python
Sequence translation	FASTA, NEXUS, PHYLIP	CLI / GUI / Python

Contribution

We welcome any contribution, from issue reporting and ideas to improve the app and documentation to code contribution. For ideas and issue reporting, please post on the Github issues page. For code contribution, please fork the repository and send pull requests to this repository.