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avoid prefix in output #15

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rcosentino opened this issue Jan 11, 2023 · 3 comments
Open

avoid prefix in output #15

rcosentino opened this issue Jan 11, 2023 · 3 comments

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@rcosentino
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Hi, is it possible to avoid the additional prefix when demultiplexing fastq files? I would like the output files to have as prefix the name assigned in the index file. When i put a folder name as output, all the files end up starting with underscore.

@grenaud
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grenaud commented Feb 9, 2023

My apologies for the delay in replying. Can you try:
/path/directory/fileprefix
?

@rcosentino
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Thanks for your reply. I don't understand your suggestion. But I think the problem is that i was not clear in my question.
i have and index file like this:

#index1 index2 Name
ACAT GACT Sample1
GACT CTGA Sample2

I would like the demultiplexed fastq files to end up in a folder named "demultiplexed" and the demultiplexed files to be named "Sample1_r1.fq.gz" ; "Sample1_r2.fq.gz" ; "Sample2_r1.fq.gz" and "Sample2_r2.fq.gz". So i run the following deML command:
deML -i index_info.txt -f read1.fq.gz -r read2.fq.gz -if1 iread1.fq.gz -if2 iread2.fq.gz -o ./demultiplexed/

The output files end up in the correct folder but they have and underscore at the beginning (e.g. "_Sample1_r1.fq.gz"). Is there any way to avoid that underscore?

@grenaud
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grenaud commented Mar 28, 2023

My apologies, I should have been more verbose, I means that if you want as output:
/path/directory/fileprefix use
-o /path/directory/fileprefix

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