diff --git a/.changeset/olive-loops-look.md b/.changeset/olive-loops-look.md
new file mode 100644
index 0000000000000..c38de2373ec78
--- /dev/null
+++ b/.changeset/olive-loops-look.md
@@ -0,0 +1,5 @@
+---
+"gradio": patch
+---
+
+fix:Allow `gr.Request` to work with ZeroGPU
diff --git a/gradio/route_utils.py b/gradio/route_utils.py
index 4aa70f6491c20..f5da757b2a36a 100644
--- a/gradio/route_utils.py
+++ b/gradio/route_utils.py
@@ -6,6 +6,7 @@
 import hmac
 import json
 import os
+import pickle
 import re
 import shutil
 import sys
@@ -18,6 +19,7 @@
 from tempfile import NamedTemporaryFile, _TemporaryFileWrapper
 from typing import (
     TYPE_CHECKING,
+    Any,
     AsyncContextManager,
     AsyncGenerator,
     BinaryIO,
@@ -125,15 +127,18 @@ class Request:
     query parameters and other information about the request from within the prediction
     function. The class is a thin wrapper around the fastapi.Request class. Attributes
     of this class include: `headers`, `client`, `query_params`, `session_hash`, and `path_params`. If
-    auth is enabled, the `username` attribute can be used to get the logged in user.
+    auth is enabled, the `username` attribute can be used to get the logged in user. In some environments,
+    the dict-like attributes (e.g. `requests.headers`, `requests.query_params`) of this class are automatically
+    converted to to dictionaries, so we recommend converting them to dictionaries before accessing
+    attributes for consistent behavior in different environments.
     Example:
         import gradio as gr
         def echo(text, request: gr.Request):
             if request:
-                print("Request headers dictionary:", request.headers)
-                print("IP address:", request.client.host)
+                print("Request headers dictionary:", dict(request.headers))
                 print("Query parameters:", dict(request.query_params))
-                print("Session hash:", request.session_hash)
+                print("IP address:", request.client.host)
+                print("Gradio session hash:", request.session_hash)
             return text
         io = gr.Interface(echo, "textbox", "textbox").launch()
     Demos: request_ip_headers
@@ -156,8 +161,8 @@ def __init__(
         """
         self.request = request
         self.username = username
-        self.session_hash = session_hash
-        self.kwargs: dict = kwargs
+        self.session_hash: str | None = session_hash
+        self.kwargs: dict[str, Any] = kwargs
 
     def dict_to_obj(self, d):
         if isinstance(d, dict):
@@ -165,7 +170,7 @@ def dict_to_obj(self, d):
         else:
             return d
 
-    def __getattr__(self, name):
+    def __getattr__(self, name: str):
         if self.request:
             return self.dict_to_obj(getattr(self.request, name))
         else:
@@ -177,6 +182,34 @@ def __getattr__(self, name):
                 ) from ke
             return self.dict_to_obj(obj)
 
+    def __getstate__(self) -> dict[str, Any]:
+        self.kwargs.update(
+            {
+                "headers": dict(getattr(self, "headers", {})),
+                "query_params": dict(getattr(self, "query_params", {})),
+                "cookies": dict(getattr(self, "cookies", {})),
+                "path_params": dict(getattr(self, "path_params", {})),
+                "client": {
+                    "host": getattr(self, "client", {}) and self.client.host,
+                    "port": getattr(self, "client", {}) and self.client.port,
+                },
+                "url": getattr(self, "url", ""),
+            }
+        )
+        if request_state := hasattr(self, "state"):
+            try:
+                pickle.dumps(request_state)
+                self.kwargs["request_state"] = request_state
+            except pickle.PicklingError:
+                pass
+        self.request = None
+        return self.__dict__
+
+    def __setstate__(self, state: dict[str, Any]):
+        if request_state := state.pop("request_state", None):
+            self.state = request_state
+        self.__dict__ = state
+
 
 class FnIndexInferError(Exception):
     pass
diff --git a/test/test_routes.py b/test/test_routes.py
index 8de8f7d38995e..0a01e37d5c8ad 100644
--- a/test/test_routes.py
+++ b/test/test_routes.py
@@ -2,6 +2,7 @@
 
 import functools
 import os
+import pickle
 import tempfile
 import time
 from contextlib import asynccontextmanager, closing
@@ -818,6 +819,32 @@ def identity(name, request: gr.Request):
         output = dict(response.json())
         assert output["data"] == ["test"]
 
+    def test_request_is_pickleable(self):
+        """
+        For ZeroGPU, we need to ensure that the gr.Request object is pickle-able.
+        """
+
+        def identity(name, request: gr.Request):
+            pickled = pickle.dumps(request)
+            unpickled = pickle.loads(pickled)
+            assert request.client.host == unpickled.client.host
+            assert request.client.port == unpickled.client.port
+            assert dict(request.query_params) == dict(unpickled.query_params)
+            assert request.query_params["a"] == unpickled.query_params["a"]
+            assert dict(request.headers) == dict(unpickled.headers)
+            assert request.username == unpickled.username
+            return name
+
+        app, _, _ = gr.Interface(identity, "textbox", "textbox").launch(
+            prevent_thread_lock=True,
+        )
+        client = TestClient(app)
+
+        response = client.post("/api/predict?a=b", json={"data": ["test"]})
+        assert response.status_code == 200
+        output = dict(response.json())
+        assert output["data"] == ["test"]
+
 
 def test_predict_route_is_blocked_if_api_open_false():
     io = Interface(lambda x: x, "text", "text", examples=[["freddy"]]).queue(