diff --git a/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/common.cfg b/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/common.cfg
new file mode 100644
index 000000000..5bb4e1a2c
--- /dev/null
+++ b/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/common.cfg
@@ -0,0 +1,51 @@
+# Format: //devtools/kokoro/config/proto/build.proto
+
+# Build logs will be here
+action {
+  define_artifacts {
+    regex: "**/*sponge_log.xml"
+  }
+}
+
+# Specify which tests to run
+env_vars: {
+    key: "RUN_TESTS_SESSION"
+    value: "py-3.10"
+}
+
+# Declare build specific Cloud project.
+env_vars: {
+    key: "BUILD_SPECIFIC_GCLOUD_PROJECT"
+    value: "python-docs-samples-tests-310"
+}
+
+env_vars: {
+    key: "TRAMPOLINE_BUILD_FILE"
+    value: "github/{{ metadata['repo']['repo'].split('/')[1] }}/.kokoro/test-samples.sh"
+}
+{% if custom_samples_dockerfile %}
+env_vars: {
+    key: "TRAMPOLINE_IMAGE"
+    value: "gcr.io/cloud-devrel-kokoro-resources/{{ metadata['repo']['repo'].split('/')[1] }}-samples-docker"
+}
+
+# Configure the docker image for kokoro-trampoline.
+env_vars: {
+    key: "TRAMPOLINE_DOCKERFILE"
+    value: ".kokoro/docker/samples/Dockerfile"
+}
+{% else %}
+# Configure the docker image for kokoro-trampoline.
+env_vars: {
+    key: "TRAMPOLINE_IMAGE"
+    value: "gcr.io/cloud-devrel-kokoro-resources/python-samples-testing-docker"
+}
+{% endif %}
+# Download secrets for samples
+gfile_resources: "/bigstore/cloud-devrel-kokoro-resources/python-docs-samples"
+
+# Download trampoline resources.
+gfile_resources: "/bigstore/cloud-devrel-kokoro-resources/trampoline"
+
+# Use the trampoline script to run in docker.
+build_file: "{{ metadata['repo']['repo'].split('/')[1] }}/.kokoro/trampoline_v2.sh"
\ No newline at end of file
diff --git a/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/continuous.cfg b/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/continuous.cfg
new file mode 100644
index 000000000..a1c8d9759
--- /dev/null
+++ b/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/continuous.cfg
@@ -0,0 +1,6 @@
+# Format: //devtools/kokoro/config/proto/build.proto
+
+env_vars: {
+    key: "INSTALL_LIBRARY_FROM_SOURCE"
+    value: "True"
+}
\ No newline at end of file
diff --git a/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/periodic-head.cfg b/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/periodic-head.cfg
new file mode 100644
index 000000000..da31f1cdf
--- /dev/null
+++ b/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/periodic-head.cfg
@@ -0,0 +1,18 @@
+# Format: //devtools/kokoro/config/proto/build.proto
+
+env_vars: {
+    key: "INSTALL_LIBRARY_FROM_SOURCE"
+    value: "True"
+}
+
+env_vars: {
+    key: "TRAMPOLINE_BUILD_FILE"
+    value: "github/{{ metadata['repo']['repo'].split('/')[1] }}/.kokoro/test-samples-against-head.sh"
+}
+{% if custom_samples_dockerfile %}
+# Upload the docker image after successful builds.
+env_vars: {
+    key: "TRAMPOLINE_IMAGE_UPLOAD"
+    value: "true"
+}
+{% endif %}
\ No newline at end of file
diff --git a/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/periodic.cfg b/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/periodic.cfg
new file mode 100644
index 000000000..f7ea9d9b9
--- /dev/null
+++ b/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/periodic.cfg
@@ -0,0 +1,13 @@
+# Format: //devtools/kokoro/config/proto/build.proto
+
+env_vars: {
+    key: "INSTALL_LIBRARY_FROM_SOURCE"
+    value: "False"
+}
+{% if custom_samples_dockerfile %}
+# Upload the docker image after successful builds.
+env_vars: {
+    key: "TRAMPOLINE_IMAGE_UPLOAD"
+    value: "true"
+}
+{% endif %}
\ No newline at end of file
diff --git a/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/presubmit.cfg b/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/presubmit.cfg
new file mode 100644
index 000000000..a1c8d9759
--- /dev/null
+++ b/synthtool/gcp/templates/python_library/.kokoro/samples/python3.10/presubmit.cfg
@@ -0,0 +1,6 @@
+# Format: //devtools/kokoro/config/proto/build.proto
+
+env_vars: {
+    key: "INSTALL_LIBRARY_FROM_SOURCE"
+    value: "True"
+}
\ No newline at end of file
diff --git a/synthtool/gcp/templates/python_samples/noxfile.py.j2 b/synthtool/gcp/templates/python_samples/noxfile.py.j2
index 1fd8956fb..93a9122cc 100644
--- a/synthtool/gcp/templates/python_samples/noxfile.py.j2
+++ b/synthtool/gcp/templates/python_samples/noxfile.py.j2
@@ -87,7 +87,7 @@ def get_pytest_env_vars() -> Dict[str, str]:
 
 # DO NOT EDIT - automatically generated.
 # All versions used to test samples.
-ALL_VERSIONS = ["3.6", "3.7", "3.8", "3.9"]
+ALL_VERSIONS = ["3.6", "3.7", "3.8", "3.9", "3.10"]
 
 # Any default versions that should be ignored.
 IGNORED_VERSIONS = TEST_CONFIG["ignored_versions"]