diff --git a/synthtool/gcp/templates/python_samples/noxfile.py.j2 b/synthtool/gcp/templates/python_samples/noxfile.py.j2
index bca0522ec..97bf7da80 100644
--- a/synthtool/gcp/templates/python_samples/noxfile.py.j2
+++ b/synthtool/gcp/templates/python_samples/noxfile.py.j2
@@ -85,7 +85,7 @@ def get_pytest_env_vars() -> Dict[str, str]:
 
 # DO NOT EDIT - automatically generated.
 # All versions used to tested samples.
-ALL_VERSIONS = ["2.7", "3.6", "3.7", "3.8"]
+ALL_VERSIONS = ["2.7", "3.6", "3.7", "3.8", "3.9"]
 
 # Any default versions that should be ignored.
 IGNORED_VERSIONS = TEST_CONFIG['ignored_versions']