Query Sequences (Probes)

A subset of sequences from the RSL should be selected as probes. The entire reference library can be used - in which case there is no need to create a separate file – but it is often sufficient to use only a subset of sequences from the reference sequence library, in which case, a separate file containing this subset should be created. The path to this file is specified in the DIGS control file.

NOTE: DIGS can utilize both polypeptide and nucleotide sequences within the same project and database, but separate probe and reference files should be created for nucleotide and polypeptide sequences, and separate screens using distinct control file parameters should be performed for each.