diff --git a/code/02-bulk_comparisons.Rmd b/code/02-bulk_comparisons.Rmd index 68fc4fc..1af60ac 100644 --- a/code/02-bulk_comparisons.Rmd +++ b/code/02-bulk_comparisons.Rmd @@ -242,6 +242,21 @@ save(data_chip_stats, file = glue("{out}/data_chip_stats.Rda")) ``` +Count samples: + +```{r count_samples} + +data_chip_tidy %>% + left_join(meta %>% distinct(ID_paper, Group), by = "ID_paper") %>% + distinct(ID_paper, Group, Mark) %>% + group_by(Group, Mark) %>% + count() %>% + filter(Group %in% c("HGG-H3.1/2K27M-Pons", + "HGG-H3.3K27M-Pons", + "HGG-H3.3K27M-Thal.")) + +``` + ## Scatterplot for H3.1 vs H3.3 pons Here, we'll combine the DGE data with each of different source of ChIP-seq analysis, diff --git a/code/02-bulk_comparisons.html b/code/02-bulk_comparisons.html index 5c23dc5..2010ecb 100644 --- a/code/02-bulk_comparisons.html +++ b/code/02-bulk_comparisons.html @@ -190,7 +190,7 @@

Selin Jessa []

-

08 September, 2022

+

18 October, 2022

@@ -400,6 +400,20 @@

6.1 Load data

pivot_wider(names_from = statistic, values_from = value) save(data_chip_stats, file = glue("{out}/data_chip_stats.Rda")) +

Count samples:

+
data_chip_tidy %>%
+    left_join(meta %>% distinct(ID_paper, Group), by = "ID_paper") %>%
+    distinct(ID_paper, Group, Mark) %>%
+    group_by(Group, Mark) %>%
+    count() %>% 
+    filter(Group %in% c("HGG-H3.1/2K27M-Pons",
+                        "HGG-H3.3K27M-Pons",
+                        "HGG-H3.3K27M-Thal."))
+
+ +

6.2 Scatterplot for H3.1 vs H3.3 pons

@@ -496,11 +510,11 @@

6.3 Scatterplot for H3.3 pons vs

7 Reproducibility

This document was last rendered on:

-
## 2022-09-08 15:08:33
+
## 2022-10-18 14:17:13

The git repository and last commit:

## Local:    master /lustre06/project/6004736/sjessa/from_narval/HGG-oncohistones/public
 ## Remote:   master @ origin (git@github.com:fungenomics/HGG-oncohistones.git)
-## Head:     [4101e76] 2022-09-08: Update README.md
+## Head: [8082a4d] 2022-10-18: Merge branch 'master' of github.com:fungenomics/HGG-oncohistones

The random seed was set with set.seed(100)

The R session info:

## Error in get(genname, envir = envir) : object 'testthat_print' not found
@@ -514,7 +528,7 @@

7 Reproducibility

## collate en_CA.UTF-8 ## ctype en_CA.UTF-8 ## tz EST5EDT -## date 2022-09-08 +## date 2022-10-18 ## ## ─ Packages ─────────────────────────────────────────────────────────────────── ## ! package * version date lib @@ -539,7 +553,6 @@

7 Reproducibility

## P ellipsis 0.3.2 2021-04-29 [?] ## P evaluate 0.14 2019-05-28 [?] ## P fansi 0.4.2 2021-01-15 [?] -## P farver 2.1.0 2021-02-28 [?] ## P fastmap 1.1.0 2021-01-25 [?] ## P feather * 0.3.5 2019-09-15 [?] ## P fitdistrplus 1.1-3 2020-12-05 [?] @@ -557,7 +570,6 @@

7 Reproducibility

## P gridExtra 2.3 2017-09-09 [?] ## P gtable 0.3.0 2019-03-25 [?] ## P here * 0.1 2017-05-28 [?] -## P highr 0.9 2021-04-16 [?] ## P hms 1.0.0 2021-01-13 [?] ## P htmltools 0.5.1.1 2021-01-22 [?] ## P htmlwidgets 1.5.3 2020-12-10 [?] @@ -571,7 +583,6 @@

7 Reproducibility

## P jsonlite 1.7.2 2020-12-09 [?] ## P KernSmooth 2.23-15 2015-06-29 [?] ## P knitr 1.33 2021-04-24 [?] -## P labeling 0.4.2 2020-10-20 [?] ## P later 1.0.0 2019-10-04 [?] ## P lattice 0.20-44 2021-05-02 [?] ## P lazyeval 0.2.2 2019-03-15 [?] @@ -698,8 +709,6 @@

7 Reproducibility

## CRAN (R 3.6.1) ## CRAN (R 3.6.1) ## CRAN (R 3.6.1) -## CRAN (R 3.6.1) -## CRAN (R 3.6.1) ## Github (fungenomics/icytobox@730e8b8) ## CRAN (R 3.6.1) ## CRAN (R 3.6.1) @@ -772,7 +781,6 @@

7 Reproducibility

## CRAN (R 3.6.1) ## CRAN (R 3.6.1) ## CRAN (R 3.6.1) -## CRAN (R 3.6.1) ## Github (rmflight/testrmd@0735c20) ## CRAN (R 3.6.1) ## CRAN (R 3.6.1) @@ -791,14 +799,14 @@

7 Reproducibility

## CRAN (R 3.6.1) ## ## [1] /lustre06/project/6004736/sjessa/from_narval/HGG-oncohistones/public/renv/library/R-3.6/x86_64-pc-linux-gnu -## [2] /tmp/RtmpPXZ49F/renv-system-library +## [2] /tmp/Rtmp7TyGB4/renv-system-library ## ## P ── Loaded and on-disk path mismatch.

The resources requested when this document was last rendered:

## #SBATCH --time=01:00:00
 ## #SBATCH --cpus-per-task=1
-## #SBATCH --mem=10G
+## #SBATCH --mem=20G
diff --git a/code/02-bulk_comparisons.md b/code/02-bulk_comparisons.md index 9868e5d..93ff260 100644 --- a/code/02-bulk_comparisons.md +++ b/code/02-bulk_comparisons.md @@ -1,7 +1,7 @@ --- title: "02 - Bulk RNAseq/ChIPseq comparisons" author: "Selin Jessa [[selin.jessa@mail.mcgill.ca](mailto:selin.jessa@mail.mcgill.ca)]" -date: "08 September, 2022" +date: "18 October, 2022" params: resources: "NOT SPECIFIED" output: @@ -410,6 +410,27 @@ save(data_chip_stats, file = glue("{out}/data_chip_stats.Rda")) +Count samples: + + + +```r +data_chip_tidy %>% + left_join(meta %>% distinct(ID_paper, Group), by = "ID_paper") %>% + distinct(ID_paper, Group, Mark) %>% + group_by(Group, Mark) %>% + count() %>% + filter(Group %in% c("HGG-H3.1/2K27M-Pons", + "HGG-H3.3K27M-Pons", + "HGG-H3.3K27M-Thal.")) +``` + +
+ +
+ ## Scatterplot for H3.1 vs H3.3 pons Here, we'll combine the DGE data with each of different source of ChIP-seq analysis, @@ -586,7 +607,7 @@ This document was last rendered on: ``` -## 2022-09-08 15:08:33 +## 2022-10-18 14:17:13 ``` @@ -598,7 +619,7 @@ The git repository and last commit: ``` ## Local: master /lustre06/project/6004736/sjessa/from_narval/HGG-oncohistones/public ## Remote: master @ origin (git@github.com:fungenomics/HGG-oncohistones.git) -## Head: [4101e76] 2022-09-08: Update README.md +## Head: [8082a4d] 2022-10-18: Merge branch 'master' of github.com:fungenomics/HGG-oncohistones ``` @@ -627,7 +648,7 @@ The R session info: ## collate en_CA.UTF-8 ## ctype en_CA.UTF-8 ## tz EST5EDT -## date 2022-09-08 +## date 2022-10-18 ## ## ─ Packages ─────────────────────────────────────────────────────────────────── ## ! package * version date lib @@ -652,7 +673,6 @@ The R session info: ## P ellipsis 0.3.2 2021-04-29 [?] ## P evaluate 0.14 2019-05-28 [?] ## P fansi 0.4.2 2021-01-15 [?] -## P farver 2.1.0 2021-02-28 [?] ## P fastmap 1.1.0 2021-01-25 [?] ## P feather * 0.3.5 2019-09-15 [?] ## P fitdistrplus 1.1-3 2020-12-05 [?] @@ -670,7 +690,6 @@ The R session info: ## P gridExtra 2.3 2017-09-09 [?] ## P gtable 0.3.0 2019-03-25 [?] ## P here * 0.1 2017-05-28 [?] -## P highr 0.9 2021-04-16 [?] ## P hms 1.0.0 2021-01-13 [?] ## P htmltools 0.5.1.1 2021-01-22 [?] ## P htmlwidgets 1.5.3 2020-12-10 [?] @@ -684,7 +703,6 @@ The R session info: ## P jsonlite 1.7.2 2020-12-09 [?] ## P KernSmooth 2.23-15 2015-06-29 [?] ## P knitr 1.33 2021-04-24 [?] -## P labeling 0.4.2 2020-10-20 [?] ## P later 1.0.0 2019-10-04 [?] ## P lattice 0.20-44 2021-05-02 [?] ## P lazyeval 0.2.2 2019-03-15 [?] @@ -811,8 +829,6 @@ The R session info: ## CRAN (R 3.6.1) ## CRAN (R 3.6.1) ## CRAN (R 3.6.1) -## CRAN (R 3.6.1) -## CRAN (R 3.6.1) ## Github (fungenomics/icytobox@730e8b8) ## CRAN (R 3.6.1) ## CRAN (R 3.6.1) @@ -885,7 +901,6 @@ The R session info: ## CRAN (R 3.6.1) ## CRAN (R 3.6.1) ## CRAN (R 3.6.1) -## CRAN (R 3.6.1) ## Github (rmflight/testrmd@0735c20) ## CRAN (R 3.6.1) ## CRAN (R 3.6.1) @@ -904,7 +919,7 @@ The R session info: ## CRAN (R 3.6.1) ## ## [1] /lustre06/project/6004736/sjessa/from_narval/HGG-oncohistones/public/renv/library/R-3.6/x86_64-pc-linux-gnu -## [2] /tmp/RtmpPXZ49F/renv-system-library +## [2] /tmp/Rtmp7TyGB4/renv-system-library ## ## P ── Loaded and on-disk path mismatch. ``` @@ -920,7 +935,7 @@ The resources requested when this document was last rendered: ``` ## #SBATCH --time=01:00:00 ## #SBATCH --cpus-per-task=1 -## #SBATCH --mem=10G +## #SBATCH --mem=20G ```