diff --git a/Marlin/src/inc/Conditionals_adv.h b/Marlin/src/inc/Conditionals_adv.h
index baba95267ecf..b56fa7d3f08f 100644
--- a/Marlin/src/inc/Conditionals_adv.h
+++ b/Marlin/src/inc/Conditionals_adv.h
@@ -1240,7 +1240,7 @@
 #if LCD_IS_SERIAL_HOST && !defined(LCD_SERIAL_PORT)
   #if MB(BTT_SKR_MINI_E3_V1_0, BTT_SKR_MINI_E3_V1_2, BTT_SKR_MINI_E3_V2_0, BTT_SKR_MINI_E3_V3_0, BTT_SKR_E3_TURBO, BTT_OCTOPUS_V1_1)
     #define LCD_SERIAL_PORT 1
-  #elif MB(CREALITY_V24S1_301, CREALITY_V24S1_301F4, CREALITY_V423, MKS_ROBIN, PANOWIN_CUTLASS, KODAMA_BARDO)
+  #elif MB(CREALITY_V24S1_301, CREALITY_V24S1_301F4, CREALITY_F401RE, CREALITY_V423, MKS_ROBIN, PANOWIN_CUTLASS, KODAMA_BARDO)
     #define LCD_SERIAL_PORT 2
   #else
     #define LCD_SERIAL_PORT 3
diff --git a/Marlin/src/pins/pins.h b/Marlin/src/pins/pins.h
index a631c4df99a3..d8fe329761e1 100644
--- a/Marlin/src/pins/pins.h
+++ b/Marlin/src/pins/pins.h
@@ -793,7 +793,7 @@
 #elif MB(ARTILLERY_RUBY)
   #include "stm32f4/pins_ARTILLERY_RUBY.h"          // STM32F4                              env:Artillery_Ruby
 #elif MB(CREALITY_V24S1_301F4)
-  #include "stm32f4/pins_CREALITY_V24S1_301F4.h"    // STM32F4                              env:STM32F401RC_creality env:STM32F401RC_creality_jlink env:STM32F401RC_creality_stlink
+  #include "stm32f4/pins_CREALITY_V24S1_301F4.h"    // STM32F4                              env:STM32F401RC_creality env:STM32F401RC_creality_nobootloader env:STM32F401RC_creality_jlink env:STM32F401RC_creality_stlink
 #elif MB(OPULO_LUMEN_REV4)
   #include "stm32f4/pins_OPULO_LUMEN_REV4.h"        // STM32F4                              env:Opulo_Lumen_REV4
 #elif MB(FYSETC_SPIDER_KING407)
diff --git a/ini/stm32f4.ini b/ini/stm32f4.ini
index 99b2685787e7..5582a6349a78 100644
--- a/ini/stm32f4.ini
+++ b/ini/stm32f4.ini
@@ -681,19 +681,29 @@ extra_scripts = ${common_stm32.extra_scripts}
 #
 # Ender-3 S1 STM32F401RC_creality
 #
-[env:STM32F401RC_creality]
+[STM32F401RC_creality_base]
 extends                     = stm32_variant
 board                       = genericSTM32F401RC
 board_build.variant         = MARLIN_CREALITY_STM32F401RC
-board_build.offset          = 0x10000
-board_upload.offset_address = 0x08010000
-board_build.rename          = firmware-{date}-{time}.bin
 build_flags                 = ${stm32_variant.build_flags} -DMCU_STM32F401RC -DSTM32F4
                               -DSS_TIMER=4 -DTIMER_SERVO=TIM5
                               -DENABLE_HWSERIAL3 -DTRANSFER_CLOCK_DIV=8
 build_unflags               = ${stm32_variant.build_unflags} -DUSBCON -DUSBD_USE_CDC
 monitor_speed               = 115200
 
+[env:STM32F401RC_creality]
+extends                     = STM32F401RC_creality_base
+board_build.offset          = 0x10000
+board_upload.offset_address = 0x08010000
+board_build.rename          = firmware-{date}-{time}.bin
+
+[env:STM32F401RC_creality_nobootloader]
+extends                     = STM32F401RC_creality_base
+board_build.offset          = 0x0000
+board_upload.offset_address = 0x08000000
+debug_tool                  = stlink
+upload_protocol             = stlink
+
 [env:STM32F401RC_creality_jlink]
 extends         = env:STM32F401RC_creality
 debug_tool      = jlink