write-trimmomatic.py

#! /usr/bin/env python
# This file is part of khmer, https://github.com/dib-lab/khmer/, and is
# Copyright (C) 2013-2015, Michigan State University.
# Copyright (C) 2015, The Regents of the University of California.
#
# Redistribution and use in source and binary forms, with or without
# modification, are permitted provided that the following conditions are
# met:
#
#     * Redistributions of source code must retain the above copyright
#       notice, this list of conditions and the following disclaimer.
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#     * Redistributions in binary form must reproduce the above
#       copyright notice, this list of conditions and the following
#       disclaimer in the documentation and/or other materials provided
#       with the distribution.
#
#     * Neither the name of the Michigan State University nor the names
#       of its contributors may be used to endorse or promote products
#       derived from this software without specific prior written
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# THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS
# "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
# LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR
# A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT
# HOLDER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL,
# SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT
# LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE,
# DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
# THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT
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# Contact: khmer-project@idyll.org

import glob

filelist = glob.glob('*R1*.fastq.gz')

for r1 in filelist:
    r2 = r1.replace('R1', 'R2')
    final_pe = r1[:-9] + '.pe.fq.gz'
    final_se = r1[:-9] + '.se.fq.gz'
    print("""\
mkdir trim
cd trim
java -jar /usr/local/bin/trimmomatic-0.30.jar PE ../%s ../%s s1_pe s1_se s2_pe s2_se ILLUMINACLIP:/usr/local/share/adapters/TruSeq3-PE.fa:2:30:10
/usr/local/share/khmer/scripts/interleave-reads.py s1_pe s2_pe | gzip -9c > ../%s

cat s1_se s2_se | gzip -9c > ../%s
cd ..
rm -r ./trim/

chmod u-w %s %s
""" % (r1, r2, final_pe, final_se, final_pe, final_se))