diff --git a/requirements.txt b/requirements.txt
index 41f6656..b631737 100644
--- a/requirements.txt
+++ b/requirements.txt
@@ -6,3 +6,4 @@ pysam==0.21.0
 numpy==1.24.3
 pandas==2.0.1
 pytest
+truvari
\ No newline at end of file
diff --git a/scripts/Spike/2b_SV.sh b/scripts/Spike/2b_SV.sh
index b7527bd..7f88676 100644
--- a/scripts/Spike/2b_SV.sh
+++ b/scripts/Spike/2b_SV.sh
@@ -8,15 +8,15 @@
 
 READ_LENGTH=$1
 VAF=$2
-VCF_A=$3
-VCF_B=$4
-MOD_BAM=$5
-OUTPUT_VCF=$6
-REFERENCE=$7
-OUTPUT_PFX=$8
-
-echo "VCF_A: $VCF_A"
-echo "VCF_B: $VCF_B"
+#$VCF_A=$3
+#VCF_B=$4
+MERGED_VCF=$3
+MOD_BAM=$4
+OUTPUT_VCF=$5
+REFERENCE=$6
+OUTPUT_PFX=$7
+
+echo "MERGED_VCF: $MERGED_VCF"
 echo "MOD_BAM: $MOD_BAM"
 
 if [ "$READ_LENGTH" = "short" ]; then
@@ -28,11 +28,7 @@ if [ "$READ_LENGTH" = "short" ]; then
 
 elif [ "$READ_LENGTH" = "long" ]; then
     echo "Input is 'long' starting Sniffles2"
-    sniffles --input $MOD_BAM --genotype-vcf $VCF_A --sample-id mosaicSim --vcf "${OUTPUT_VCF}.vcf1.vcf"
-    sniffles --input $MOD_BAM --genotype-vcf $VCF_B --sample-id mosaicSim --vcf "${OUTPUT_VCF}.vcf2.vcf"
-
-    # Merge the two VCF files with bcftools
-    bcftools merge --force-samples -O z --write-index -o $OUTPUT_VCF $VCF_A $VCF_B
+    sniffles --input $MOD_BAM --genotype-vcf $MERGED_VCF --sample-id mosaicSim --vcf "${OUTPUT_VCF}"
 
 else
     echo "Error: Invalid input"
diff --git a/scripts/Spike/2b_re-genotyping_main.sh b/scripts/Spike/2b_re-genotyping_main.sh
index e0a2bfa..c8dad1a 100644
--- a/scripts/Spike/2b_re-genotyping_main.sh
+++ b/scripts/Spike/2b_re-genotyping_main.sh
@@ -20,7 +20,9 @@ MOD_BAM=$5
 OUTPUT_DIR=$6
 READ_LENGTH=$7
 MERGED_VCF="${OUTPUT_DIR}/merged.vcf.gz"
-OUTPUT_VCF="${OUTPUT_DIR}/output_genotypes.vcf"
+COLLAPSED_MERGED_VCF="${OUTPUT_DIR}/collapsed_merged.vcf.gz"
+COLLAPSED_VARIANTS_VCF="${OUTPUT_DIR}/collapsed_variants.vcf.gz"
+OUTPUT_VCF="${OUTPUT_DIR}/output_regenotyped.vcf.gz"
 OUTPUT_VCF_FILTERED="${OUTPUT_DIR}/output_genotypes_filtered.vcf"
 REFERENCE=${8}
 
@@ -28,13 +30,15 @@ mkdir -p $OUTPUT_DIR
 
 # Merge the two VCF files with bcf tools
 bcftools merge --force-samples -O z --write-index -o $MERGED_VCF $VCF_A $VCF_B
+# Collapse variants with truvari
+truvari collapse -i $MERGED_VCF -o $COLLAPSED_MERGED_VCF -c $COLLAPSED_VARIANTS_VCF
 
 if [ "$VARIANT" = "SNV" ]; then
     echo "Input is 'SNV'"
-    ./2b_SNV.sh $READ_LENGTH $VAF $MERGED_VCF $MOD_BAM $OUTPUT_VCF $REFERENCE
+    ./2b_SNV.sh $READ_LENGTH $VAF $COLLAPSED_MERGED_VCF $MOD_BAM $OUTPUT_VCF $REFERENCE
 elif [ "$VARIANT" = "SV" ]; then
     echo "Input is 'SV'"
-    ./2b_SV2.sh $READ_LENGTH $VAF $VCF_A $VCF_B $OUTPUT_VCF "${OUTPUT_DIR}/output_genotypes" $REFERENCE "Spike_out"
+    ./2b_SV2.sh $READ_LENGTH $VAF $COLLAPSED_MERGED_VCF $OUTPUT_VCF "${OUTPUT_DIR}/output_genotypes" $REFERENCE "Spike_out"
 else
     echo "Error: Invalid input"
 fi