diff --git a/e2e/components/Checkbox/Checkbox-test.avt.e2e.js b/e2e/components/Checkbox/Checkbox-test.avt.e2e.js
new file mode 100644
index 000000000000..846c0d6d9777
--- /dev/null
+++ b/e2e/components/Checkbox/Checkbox-test.avt.e2e.js
@@ -0,0 +1,111 @@
+/**
+ * Copyright IBM Corp. 2016, 2023
+ *
+ * This source code is licensed under the Apache-2.0 license found in the
+ * LICENSE file in the root directory of this source tree.
+ */
+
+'use strict';
+
+import { expect, test } from '@playwright/test';
+import { visitStory } from '../../test-utils/storybook';
+
+test.describe('Checkbox @avt', () => {
+  test('@avt-default-state', async ({ page }) => {
+    await visitStory(page, {
+      component: 'Checkbox',
+      id: 'components-checkbox--default',
+      globals: {
+        theme: 'white',
+      },
+    });
+    await expect(page).toHaveNoACViolations('Checkbox');
+  });
+
+  test('@avt-advanced-states single', async ({ page }) => {
+    await visitStory(page, {
+      component: 'Checkbox',
+      id: 'components-checkbox--single',
+      globals: {
+        theme: 'white',
+      },
+    });
+    await expect(page).toHaveNoACViolations('Checkbox-single');
+  });
+
+  test('@avt-advanced-states skeleton', async ({ page }) => {
+    await visitStory(page, {
+      component: 'Checkbox',
+      id: 'components-checkbox--skeleton',
+      globals: {
+        theme: 'white',
+      },
+    });
+    await expect(page).toHaveNoACViolations('Checkbox-skeleton');
+  });
+
+  test('@avt-keyboard-nav default', async ({ page }) => {
+    await visitStory(page, {
+      component: 'Checkbox',
+      id: 'components-checkbox--default',
+      globals: {
+        theme: 'white',
+      },
+    });
+    const checkbox1 = page.locator('input#checkbox-label-1');
+
+    await expect(checkbox1).toBeVisible();
+    await page.keyboard.press('Tab');
+    await expect(checkbox1).toBeFocused();
+
+    // Checking checkbox
+    await page.keyboard.press('Space');
+    await expect(checkbox1).toBeChecked();
+
+    // Unchecking checkbox
+    await page.keyboard.press('Space');
+    await expect(checkbox1).not.toBeChecked();
+  });
+
+  test('@avt-keyboard-nav single', async ({ page }) => {
+    await visitStory(page, {
+      component: 'Checkbox',
+      id: 'components-checkbox--single',
+      globals: {
+        theme: 'white',
+      },
+    });
+    const checkbox3 = page.locator('input#checkbox-3');
+    const checkbox4 = page.locator('input#checkbox-4');
+    const checkbox5 = page.locator('input#checkbox-5');
+    const checkbox6 = page.locator('input#checkbox-6');
+
+    // Check the first checkbox 3
+    await expect(checkbox3).toBeVisible();
+    await page.keyboard.press('Tab');
+    await expect(checkbox3).toBeFocused();
+    await page.keyboard.press('Space');
+    await expect(checkbox3).toBeChecked();
+
+    // Check the first checkbox 4
+    await expect(checkbox4).toBeVisible();
+    await page.keyboard.press('Tab');
+    await expect(checkbox4).toBeFocused();
+    await page.keyboard.press('Space');
+    await expect(checkbox4).toBeChecked();
+
+    // Check the first checkbox 5
+    await expect(checkbox5).toBeVisible();
+    await page.keyboard.press('Tab');
+    await expect(checkbox5).toBeFocused();
+    await page.keyboard.press('Space');
+    await expect(checkbox5).toBeChecked();
+
+    // Checking if the checkbox is readonly
+    await expect(checkbox6).toBeVisible();
+    await page.keyboard.press('Tab');
+    await expect(checkbox6).toBeFocused();
+    await page.keyboard.press('Space');
+    await expect(checkbox6).not.toBeChecked();
+  });
+});
diff --git a/e2e/components/Checkbox/Checkbox-test.e2e.js b/e2e/components/Checkbox/Checkbox-test.e2e.js
index bde867d5c46d..595f10a1fa0b 100644
--- a/e2e/components/Checkbox/Checkbox-test.e2e.js
+++ b/e2e/components/Checkbox/Checkbox-test.e2e.js
@@ -7,9 +7,9 @@
 
 'use strict';
 
-const { expect, test } = require('@playwright/test');
+const { test } = require('@playwright/test');
 const { themes } = require('../../test-utils/env');
-const { snapshotStory, visitStory } = require('../../test-utils/storybook');
+const { snapshotStory } = require('../../test-utils/storybook');
 
 test.describe('Checkbox', () => {
   themes.forEach((theme) => {
@@ -23,15 +23,4 @@ test.describe('Checkbox', () => {
       });
     });
   });
-
-  test('accessibility-checker @avt', async ({ page }) => {
-    await visitStory(page, {
-      component: 'Checkbox',
-      id: 'components-checkbox--default',
-      globals: {
-        theme: 'white',
-      },
-    });
-    await expect(page).toHaveNoACViolations('Checkbox');
-  });
 });