quick calibration for 4x and 20x objectives on cytation1 w/ 1288x964 pixel Blackfly BFLY-U3-13S2M sensor

[ben-ray]

beware not all cytations may be built equally, so if you have a different sensor, it might be worth calculating these values for your particular setup

you can find the mapping between pixels <> microns by exporting an image with scalebar from gen5.exe using your cytation & counting the pixels of the scalebar

[rickwierenga]

what is going on with these denominators?

for the cytation5, it's to convert um to mm

[ben-ray]

The cytation5 sensor is 2448x2048 while the cytation1 is 1288x964. The scaling factor from pixel to micron will be identical for both axis, but why does the image_size conversion not take the sensor size into account? I would expect the X and Y size to be different but the scaling factor is identical in X and Y in the original Cytation5 implementation.

These numbers are my attempt to account for the significantly different x and y size of my sensor (my pixels are still perfectly square)

[rickwierenga]

this is not a scaling factor

[ben-ray]

Then how do I determine these and why are they not different for X and Y on the cytation5?

[rickwierenga]

i took the number from gen5.exe, and then did um -> mm conversion which seems to work

[rickwierenga]

at least it corresponds with their firmware commands.

you can use a firmware command to check