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"Unique reads" higher than "total reads" #21

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kebarr opened this issue Jan 19, 2022 · 0 comments
Open

"Unique reads" higher than "total reads" #21

kebarr opened this issue Jan 19, 2022 · 0 comments

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@kebarr
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kebarr commented Jan 19, 2022

Please see example attached. This is for a simulated read set of a single species, and a kmer collection containing that species amongst others. How is it possible for the number of unique reads in the results table to be greater than the number of reads used for the analysis?

report_ft2.zip

I have tried to use the -o parameter to output the reads that contain kmers from my collection, but it outputs an empty file.

I have run fastv as follows:

fastv -o FT.fastq -w 16 -c unique_kmer/kmercollection.fasta -q 10 -h ./all_pathogens_crs_results/report_ft2.html -j ./all_pathogens_crs_results/report_ft2.json -i ft_lt5k.fastq

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