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FreedomScope is an umbrella project for several customizable and scaleable single-photon fluorescent imaging microscope systems that take advantage of developing open-source landscape to provide a modular in-vivo all optical neurophysiology rigs with a transparent hardware, and acquisition and analysis software.
You can read more about the project here.
The project was developed by Will Liberti in the Gardner Lab, for multi-month imaging of neural activity in songbirds [1] [2] [3] bats, and rodents. This effort would not have been possible without the critical advice and support at the outset of the project by Daniel Aharoni and Peyman Golshani, of the UCLA Miniscope project
The FinchScope is a lightweight wireless-capable microscope for biological imaging- Designed specifically for monitoring the neural activity (via genetically encoded calcium indicators) of zebra finches while they sing their courtship songs:
You can find the Construction and Assembly Guide for the Microscope, As well as a for the Data Acquisition Box (DAQ). Also, we have a Guide Through a Basic Analysis Pipeline
The FinchScope is wireless capable and is being used in bats with the Yartsev lab:
Additional resources related to this project can be found (here.)https://github.com/WALIII/ImBat
The 1P2C Miniscope is a variant of the FinchScope that incorporates a second excitation path for dual color imaging, or for widefield photo- stimulation. You can find the Construction and Assembly Guide for the Microscope, As well as a for the Data Acquisition Box (DAQ).
The WideField miniature microscope weighs 4g and gives a 4x3mm field of view. You can find the Construction and Assembly Guide for the Microscope, As well as a for the Data Acquisition Box (DAQ).
The Active Commutator is a low cost, low noise, active (driven by a motor and sensor) electrical rotary joint designed for electrophysiology (single and multichannel micro-electrode arrays) and optophysiology ( optogenetics, miniature microscopes, and fiber photometry).
If you use any part of this project in your work, please cite our Journal of Neural Engineering Paper:
- Liberti III, William A., et al. "An open source, wireless capable miniature microscope system." Journal of neural engineering 14.4 (2017): 045001.
- Liberti, William A., et al. "Unstable neurons underlie a stable learned behavior." Nature neuroscience 19.12 (2016): 1665-1671.
- Yanny, K., Antipa, N., Liberti, W., et al. (2020). Miniscope3D: optimized single-shot miniature 3D fluorescence microscopy. Light: Science & Applications, 9(1), 1-13.
- Leman, Daniel P., et al. "Large-scale cellular-resolution imaging of neural activity in freely behaving mice." (bioRxiv) (2021).
- Cohen, Yarden, et al. "Hidden neural states underlie canary song syntax." Nature 582.7813 (2020): 539-544.
- Will Liberti ( U.C. Berkeley)
- Nathan Perkins ( Apple )
- Daniel Leman ( Brandeis)
- Jasmine Clevenger ( B.U.)
- Will Yen (B.U.)