diff --git a/content/resource/biochemistrytools.md b/content/resource/biochemistrytools.md index 084fce4..6810ed3 100644 --- a/content/resource/biochemistrytools.md +++ b/content/resource/biochemistrytools.md @@ -24,8 +24,13 @@ thumbnailImagePosition: left ![randyschekman1](/img/randy_cell_enzymes_bag.jpg) --- -## Foudamental techniques -- Western blot +## Foundational techniques + +- **Protein quantification**\ +[ ] Western blot + +- **Protein purification**\ +[ ] Purifying Challenging Proteins, Principles and Methods [[PDF](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/ERJk71FTHSNElA3GMam-l3IBX6v_i_oCQeLQLG1Hb6bBiA?e=mAkBNn)] --- ## Vendor companies @@ -42,24 +47,43 @@ thumbnailImagePosition: left #### **Signaling pathway and basic science** 1. [Zhijian-James Chen lab, UTSW](https://www.james-zhijian-chen-lab.org/)\ [ ] **Discovery of cGAS-cGAMP pathway and its role in immune defense** -- Sun, Lijun, et al. "Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway." Science 339.6121 (2013): 786-791. [ [DOI Link](https://www.doi.org/10.1126/science.1232458)] +- Sun, Lijun, et al. "Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway." Science 339.6121 (2013): 786-791. [[PDF](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EWZWjSjbymdFsI777Dv2EYsBAlzY_hKLXIg1JZQzN8XxeQ?e=y4CBrs) | [PMID: 23258413](https://pubmed.ncbi.nlm.nih.gov/23258413/) | [DOI Link](https://www.doi.org/10.1126/science.1232458)] - Wu, Jiaxi, et al. "Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA." Science 339.6121 (2013): 826-830. [[PDF](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EcEtj2d-iKlGuWeCOcAUFisBiju-HRGaphC-v8jjZmznkA?e=SsslUO) | [DOI Link](https://www.doi.org/10.1126/science.1229963)] 2. [David M. Sabatini lab, WIBR, MIT](https://www.davidmsabatini.com/)\ -[ ] **The discovery of mTOR**\ -Sabatini et al. "RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs." Cell 78.1 (1994): 35-43. [[PDF]() | [DOI Link](https://doi.org/10.1016/0092-8674(94)90570-3)]\ -[ ] Systematic characterzation of basic mechanisms that regulate growth including nutrient sensing pathways. [[CASTOR](https://doi.org/10.1016/j.cell.2016.02.035) | [Sestrin2](https://doi.org/10.1126/science.aad2087) | [SAMTOR](https://doi.org/10.1126/science.aao3265)] - - - +[ ] **The discovery of mTOR** +- Sabatini et al. "RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs." Cell 78.1 (1994): 35-43. [[PDF]() | [DOI Link](https://doi.org/10.1016/0092-8674(94)90570-3)]\ +[ ] **Systematic characterzation of basic mechanisms that regulate growth including nutrient sensing pathways** [[CASTOR](https://doi.org/10.1016/j.cell.2016.02.035) | [Sestrin2](https://doi.org/10.1126/science.aad2087) | [SAMTOR](https://doi.org/10.1126/science.aao3265)] 3. [Xiaodong Wang lab, UTSW, NIBS](http://www.nibs.ac.cn/en/yjsjyimgshow.php?cid=5&sid=6&id=779)\ [ ] The discovery of mitochondria CytoC mediated apoptosis pathway +- Zou, Hua, et al. "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c–dependent activation of caspase-3." Cell 90.3 (1997): 405-413. +[[PDF](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/ERv446uXYcxCuPrmRHgWvgYBgN04_ogoRRKU8jTIB-WTEw?e=JvdhVY) | [PMID: 9267021](https://pubmed.ncbi.nlm.nih.gov/9267021/) | [DOI Link](https://doi.org/10.1016/s0092-8674(00)80501-2)] 4. [Feng Shao lab, NIBS](http://www.nibs.ac.cn/en/yjsjyimgshow.php?cid=5&sid=6&id=777)\ [ ] **The discovery of GSDMD mediated pyroptosis pathway** - Shi, Jianjin, et al. "Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death." Nature 526.7575 (2015): 660-665. [[PDF](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/ER85jPeKCyVIj24uQEX75W4BDUoOxtQY5Ap89wOXnDH6pw?e=8pOlMx) | [DOI Link](https://doi.org/10.1038/nature15514)] + +#### **Transcriptional regulation and epigenetics** +1. [Robert Tjian, University of California, Berkeley/HHMI](https://www.tjian-darzacq.mcb.berkeley.edu/tjian/)\ +[ ] **The discovery of the SV40 large T antigen** +- Tjian, Robert. "The binding site on SV40 DNA for a T antigen-related protein." Cell 13.1 (1978): 165-179. [[PMID: 202398](https://pubmed.ncbi.nlm.nih.gov/202398/) | [DOI Link](https://doi.org/10.1016/0092-8674(78)90147-2)] + +2. [Yi Zhang lab, University of North Carolina at Chapel Hill/HMS/HHMI](https://www.zhanglab.tch.harvard.edu/) + + +3. [Yang Shi lab, HMS/HHMI/University of Oxford](https://www.harvardshilab.org/)\ +[ ] **The discovery of first histone demethylase, LSD1** +- Shi, Yujiang, et al. "Histone demethylation mediated by the nuclear amine oxidase homolog LSD1." Cell 119.7 (2004): 941-953. [[PDF](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EYPS2hk_U1VCm-bMyCGIu-QBg92LUK47Nw4J3vIEapyRCQ?e=EF25wj) | [DOI Link](https://doi.org/10.1016/j.cell.2004.12.012)] + + +4. [Chuan He lab, University of Chicago/HHMI](https://he-group.uchicago.edu/He%20research.html)\ +[ ] **The discovery of first RNA demethylase that oxidatively reverses N6-methyladenosine (m6A) methylation in mammalian messenger RNA** +- Jia, Guifang, et al. "N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO." Nature chemical biology 7.12 (2011): 885-887. [[PDF](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/ETNp772T_4VNnY3909X_7lUB_8CJ36WsacqULe7ApUAzSA?e=QWFN4P) | [DOI Link](https://doi.org/10.1038/nchembio.687)] + + + #### **Tool development** 1. [Alice Ting lab, Stanford](http://www.tinglab.org/) diff --git a/content/resource/geneticstools.md b/content/resource/geneticstools.md index c6b443b..b7b2f4b 100644 --- a/content/resource/geneticstools.md +++ b/content/resource/geneticstools.md @@ -104,14 +104,12 @@ Park, Yongmin Mason, et al. "Astrocyte specificity and coverage of hGFAP-CreERT2 ---- ### Ref. public websites 1. [The Jackson Laborary](https://www.jax.org/): The laboratory is also the world's source for more than 8,000 strains of genetically defined mice, and serves as a worldwide center for scientific courses, conferences, training, and education. 2. [Mouse Genome Informatics](http://www.informatics.jax.org/): MGI is the international database resource for the laboratory mouse, providing integrated genetic, genomic, and biological data to facilitate the study of human health and disease. ---- ### Ref Labs 1. [Allen Institute for Brain Research](http://connectivity.brain-map.org/transgenic/): 2. [Susumu Tonegawa Lab](https://tonegawalab.mit.edu/susumu-tonegawa/), MIT @@ -126,29 +124,40 @@ Park, Yongmin Mason, et al. "Astrocyte specificity and coverage of hGFAP-CreERT2 + + + --- # Drosophila melanogaster +![seymour_benzer1](/img/Seymour_and_thefly.png) +Seymour Benzer with mega-Drosophila, 1974. +### Ref. Websites +1. [FlyBase](https://flybase.org/): A Database of Drosophila Genes & Genomes --- # Zebrafish +### Ref. Websites +1. [ZFIN](https://zfin.org/): The Zebrafish Information Network (ZFIN) is the database of genetic and genomic data for the zebrafish (Danio rerio) as a model organism. ZFIN provides a wide array of expertly curated, organized and cross-referenced zebrafish research data. - +--- ## Nobel laureates -- Sydney Brenner(1927-2019), The Molecular Sciences Institute, Berkeley, CA\ +- **Michael W. Young**, Rockefeller University, New York, NY, USA\ +Leading pioneer: **Seymour Benzer(1921-2007)**, California Institute of Technology [[Public Interview](https://pkueducn-my.sharepoint.com/:v:/g/personal/lijun0705_pku_edu_cn/ESTLH4bQeIVEobzIprKewX0BtTvgmcitTEOq1JAt_DVJBw?e=peh8MA) | [Read the Interview](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EZVmcpluPh9Pkayf_EYVUugB8Q9QW5Y5qCPsVW00Of3z7w?e=faAcdk)]\ +The Nobel Prize in Physiology or Medicine 2017\ +**Time Travels: A 40 Year Journey from Drosophila’s Clock Mutants to Human Circadian Disorders** [[Nobel Lecture video](https://www.youtube.com/watch?v=Blm3QwT-rRk&ab_channel=NobelPrize) | [Lecture Slides](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EQ3Lh8WjagRFoiNVbUsIbk0BbkzwPzSe2gswYWApBQQSoA?e=70flib) | [Read the Lecture](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/ERsnFw1YSGpKmK-x6I-OcY8B_SnYvmbc9TuU_qTaM_bA4Q?e=GYkHk2) | [Source](https://www.nobelprize.org/prizes/medicine/2017/young/facts/)] + +- **Sydney Brenner(1927-2019)**, The Molecular Sciences Institute, Berkeley, CA\ The Nobel Prize in Physiology or Medicine 2002\ **Nature's Gift to Science** [[Nobel Lecture video](https://pkueducn-my.sharepoint.com/:v:/g/personal/lijun0705_pku_edu_cn/Ec3SUiYnkIZAnC5VfbVEuQcBCIB9298kRxmK85J5uhUt0Q?e=Lh7u48) | [Read the Lecture](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/ERaf-DRSrGVOkrs3delRx44B9TsHkEVqe2q1iZhgTmUdgg?e=8xBNmp) | [Source](https://www.nobelprize.org/prizes/medicine/2002/brenner/biographical/)] -- Barbara McClintock(1902-1992), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA\ +- **Barbara McClintock(1902-1992)**, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA\ The Nobel Prize in Physiology or Medicine 1983\ **The Significance of Responses of the Genome to Challenge** [[Nobel Lecture video](https://pkueducn-my.sharepoint.com/:v:/g/personal/lijun0705_pku_edu_cn/Ec3SUiYnkIZAnC5VfbVEuQcBCIB9298kRxmK85J5uhUt0Q?e=Lh7u48) | [Read the Lecture](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/ERsnFw1YSGpKmK-x6I-OcY8B_SnYvmbc9TuU_qTaM_bA4Q?e=GYkHk2) | [Source](https://www.nobelprize.org/prizes/medicine/1983/mcclintock/facts/)] -- Michael W. Young, Rockefeller University, New York, NY, USA\ -Leading pioneer [Seymour Benzer](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EZVmcpluPh9Pkayf_EYVUugB8Q9QW5Y5qCPsVW00Of3z7w?e=faAcdk)\ -The Nobel Prize in Physiology or Medicine 2017\ -**Time Travels: A 40 Year Journey from Drosophila’s Clock Mutants to Human Circadian Disorders** [[Nobel Lecture video](https://www.youtube.com/watch?v=Blm3QwT-rRk&ab_channel=NobelPrize) | [Lecture Slides](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EQ3Lh8WjagRFoiNVbUsIbk0BbkzwPzSe2gswYWApBQQSoA?e=70flib) | [Read the Lecture](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/ERsnFw1YSGpKmK-x6I-OcY8B_SnYvmbc9TuU_qTaM_bA4Q?e=GYkHk2) | [Source](https://www.nobelprize.org/prizes/medicine/2017/young/facts/)] + diff --git a/content/resource/molecularbiology.md b/content/resource/molecularbiology.md index fa674e2..c5306d1 100644 --- a/content/resource/molecularbiology.md +++ b/content/resource/molecularbiology.md @@ -29,10 +29,10 @@ thumbnailImagePosition: left > --- -Brain organ specific: BR1\ +Brain organ specific: AAV-BR1[PDF | PMID: | DOI Link]\ Liver specific: -Brain endothelial cell specific: AAV-BI30 +Brain endothelial cell specific: AAV-BI30[PDF | PMID: | DOI Link] @@ -139,9 +139,10 @@ ChR2(Channelrhodopsin-2)[hChR2(H134R) | ChR2(C128A/C128S)] ## Ref Labs 1. [Feng Zhang lab, MIT](https://zlab.bio/) -2. [Karl Deisseroth lab, Stanford](https://web.stanford.edu/group/dlab/about_pi.html) -3. [Yulong Li lab, PKU](http://www.yulonglilab.org/) -4. [David Liu lab, Harvard](https://www.liugroup.us/) +2. [David Liu lab, Harvard](https://www.liugroup.us/) +3. [Karl Deisseroth lab, Stanford](https://web.stanford.edu/group/dlab/about_pi.html) +4. [Yulong Li lab, PKU](http://www.yulonglilab.org/) +5. [Jin Zhang lab, UCSD](http://jinzhanglab.ucsd.edu/) --- ## Nobel laureates @@ -165,6 +166,13 @@ The Nobel Prize in Chemistry 2008\ The Nobel Prize in Chemistry 2008\ **Constructing and Exploiting the Fluorescent Protein Paintbox** [[Nobel Lecture video](https://pkueducn-my.sharepoint.com/:v:/g/personal/lijun0705_pku_edu_cn/EX_KX-KLPsFBhgk9pOovOzIBqp8mjNiJMFrchuwG72LKxg?e=9C9yCR) | [Lecture Slides](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EbXNqpxXkjpIrhgjOkKCvzAB_K3sN2tHir1mLW5t61ea4w?e=q1npQ2) | [Read the Lecture](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EVZYQT1jreBBrSaWJ-MMmU8Bja7PbbZo16NCLwFhcSoJRg?e=QwVmgh) | [Source](https://www.nobelprize.org/prizes/chemistry/2008/tsien/facts/)] +- Craig C. Mello, University of Massachusetts Medical School, Worcester, MA, USA\ +The Nobel Prize in Physiology or Medicine 2006\ +**Return to the RNAi World: Rethinking Gene Expression and Evolution** [[Nobel Lecture video](https://pkueducn-my.sharepoint.com/:v:/g/personal/lijun0705_pku_edu_cn/ERJCKx_Rxm5DqAj_FwjVRQoB514JTZqibAK96j2bjrkALg?e=z9fTmu) | [Lecture Slides](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/Eao2Yc7A65xEkWg53nQmKd8B-cRBCjfUSdwCn0yOD2cEZg?e=JcBHKl) | [Read the Lecture](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EXIuNu-X9aFBmESlD6lhERcBVK6IZ-6J-OKB5-nAM0OJfQ?e=ekQ3KO) | [Source](https://www.nobelprize.org/prizes/medicine/2006/mello/facts/)] + +- Andrew Z. Fire, Stanford University School of Medicine, Stanford, CA, USA\ +The Nobel Prize in Physiology or Medicine 2006\ +**Gene Silencing by Double Stranded RNA** [[Nobel Lecture video](https://pkueducn-my.sharepoint.com/:v:/g/personal/lijun0705_pku_edu_cn/ERJCKx_Rxm5DqAj_FwjVRQoB514JTZqibAK96j2bjrkALg?e=z9fTmu) | [Lecture Slides](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/Eao2Yc7A65xEkWg53nQmKd8B-cRBCjfUSdwCn0yOD2cEZg?e=JcBHKl) | [Read the Lecture](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EXIuNu-X9aFBmESlD6lhERcBVK6IZ-6J-OKB5-nAM0OJfQ?e=ekQ3KO) | [Source](https://www.nobelprize.org/prizes/medicine/2006/fire/facts/)] - Phillip A. Sharp, Massachusetts Institute of Technology (MIT), Center for Cancer Research, Cambridge, MA, USA\ The Nobel Prize in Physiology or Medicine 1993\ diff --git a/public/categories/category/index.html b/public/categories/category/index.html index d1b41e7..8b1e31a 100644 --- a/public/categories/category/index.html +++ b/public/categories/category/index.html @@ -365,7 +365,9 @@

- Vendor companies Abcam ThermoFisher R&D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange +
+

Our cells engage in protein production, and many of those proteins are enzymes responsible for the chemistry of life. — Randy Schekman

+

@@ -408,15 +410,9 @@

- Fluorescence Proteins -iRFP: miRFPnano670 -RFPs: mRuby3, mCherry -GFPs: mGreenLantern, EGFP -BFPs: TagBFP2, TagBFP -Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1 -Liver specific: -Brain endothelial cell specific: AAV-BI30 -Tissue/Cell type specific promoter Pan-neuron: hSyn -Kügler, S., E. Kilic, and M. Bähr. “Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. +
+

Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. — Sydney Brenner

+

diff --git a/public/categories/category/index.xml b/public/categories/category/index.xml index d5eb411..7e2fa0d 100644 --- a/public/categories/category/index.xml +++ b/public/categories/category/index.xml @@ -23,7 +23,9 @@ Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC Thu, 25 Aug 2022 17:16:39 +0800 https://leoj.xyz/resource/biochemistrytools/ - Vendor companies Abcam ThermoFisher R&amp;D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange + <blockquote> +<p>Our cells engage in protein production, and many of those proteins are enzymes responsible for the chemistry of life. &mdash; Randy Schekman</p> +</blockquote> @@ -32,15 +34,9 @@ Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC Sun, 21 Aug 2022 20:27:51 +0800 https://leoj.xyz/resource/molecularbiology/ - Fluorescence Proteins -iRFP: miRFPnano670 -RFPs: mRuby3, mCherry -GFPs: mGreenLantern, EGFP -BFPs: TagBFP2, TagBFP -Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1 -Liver specific: -Brain endothelial cell specific: AAV-BI30 -Tissue/Cell type specific promoter Pan-neuron: hSyn -Kügler, S., E. Kilic, and M. Bähr. &ldquo;Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. + <blockquote> +<p>Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. &mdash; Sydney Brenner</p> +</blockquote> @@ -146,9 +142,8 @@ Samuel Lundt#, Nannan Zhang#, Jun-Liszt Li, Zhe Zhang, Xiaowan Wang, Ruisi Bao, Mon, 15 Aug 2022 15:15:42 +0800 https://leoj.xyz/page/about/ - Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. I ask questions in the cutting-edge field of brain vasculature system and brain metabolic system, working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, and next-generation microscopy (Lightsheet, Confocal, 2p), in order to understand the fundamental basis of brain metabolism and brain vasculature system. -Interest Areas Biological Question -Brain vasculature system + Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. My long-term goal is to discover and characterize basic mechanisms underlying brain aging and neuron growth. In addition, I working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, next-generation microscopy (Lightsheet, Confocal, 2p), and next-generation gene therapy (AAV, promoter) in order to apply these technologies to solve specific problems. +Interest Areas Biological Question diff --git a/public/categories/subcategory/index.html b/public/categories/subcategory/index.html index 5b1132c..4352aef 100644 --- a/public/categories/subcategory/index.html +++ b/public/categories/subcategory/index.html @@ -365,7 +365,9 @@

- Vendor companies Abcam ThermoFisher R&D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange +
+

Our cells engage in protein production, and many of those proteins are enzymes responsible for the chemistry of life. — Randy Schekman

+

@@ -408,15 +410,9 @@

- Fluorescence Proteins -iRFP: miRFPnano670 -RFPs: mRuby3, mCherry -GFPs: mGreenLantern, EGFP -BFPs: TagBFP2, TagBFP -Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1 -Liver specific: -Brain endothelial cell specific: AAV-BI30 -Tissue/Cell type specific promoter Pan-neuron: hSyn -Kügler, S., E. Kilic, and M. Bähr. “Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. +
+

Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. — Sydney Brenner

+

diff --git a/public/categories/subcategory/index.xml b/public/categories/subcategory/index.xml index 63a0255..aa1cb19 100644 --- a/public/categories/subcategory/index.xml +++ b/public/categories/subcategory/index.xml @@ -23,7 +23,9 @@ Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC Thu, 25 Aug 2022 17:16:39 +0800 https://leoj.xyz/resource/biochemistrytools/ - Vendor companies Abcam ThermoFisher R&amp;D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange + <blockquote> +<p>Our cells engage in protein production, and many of those proteins are enzymes responsible for the chemistry of life. &mdash; Randy Schekman</p> +</blockquote> @@ -32,15 +34,9 @@ Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC Sun, 21 Aug 2022 20:27:51 +0800 https://leoj.xyz/resource/molecularbiology/ - Fluorescence Proteins -iRFP: miRFPnano670 -RFPs: mRuby3, mCherry -GFPs: mGreenLantern, EGFP -BFPs: TagBFP2, TagBFP -Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1 -Liver specific: -Brain endothelial cell specific: AAV-BI30 -Tissue/Cell type specific promoter Pan-neuron: hSyn -Kügler, S., E. Kilic, and M. Bähr. &ldquo;Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. + <blockquote> +<p>Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. &mdash; Sydney Brenner</p> +</blockquote> @@ -146,9 +142,8 @@ Samuel Lundt#, Nannan Zhang#, Jun-Liszt Li, Zhe Zhang, Xiaowan Wang, Ruisi Bao, Mon, 15 Aug 2022 15:15:42 +0800 https://leoj.xyz/page/about/ - Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. I ask questions in the cutting-edge field of brain vasculature system and brain metabolic system, working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, and next-generation microscopy (Lightsheet, Confocal, 2p), in order to understand the fundamental basis of brain metabolism and brain vasculature system. -Interest Areas Biological Question -Brain vasculature system + Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. My long-term goal is to discover and characterize basic mechanisms underlying brain aging and neuron growth. In addition, I working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, next-generation microscopy (Lightsheet, Confocal, 2p), and next-generation gene therapy (AAV, promoter) in order to apply these technologies to solve specific problems. +Interest Areas Biological Question diff --git a/public/img/Seymour_and_thefly.png b/public/img/Seymour_and_thefly.png new file mode 100644 index 0000000..1006e1f Binary files /dev/null and b/public/img/Seymour_and_thefly.png differ diff --git a/public/img/quotes_sydney_brenner.jpg b/public/img/quotes_sydney_brenner.jpg new file mode 100644 index 0000000..b723481 Binary files /dev/null and b/public/img/quotes_sydney_brenner.jpg differ diff --git a/public/img/randy_cell_enzymes_bag.jpg b/public/img/randy_cell_enzymes_bag.jpg new file mode 100644 index 0000000..d178c94 Binary files /dev/null and b/public/img/randy_cell_enzymes_bag.jpg differ diff --git a/public/index.html b/public/index.html index 35a0d05..fc9d821 100644 --- a/public/index.html +++ b/public/index.html @@ -362,7 +362,9 @@

- Vendor companies Abcam ThermoFisher R&D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange +
+

Our cells engage in protein production, and many of those proteins are enzymes responsible for the chemistry of life. — Randy Schekman

+

@@ -405,15 +407,9 @@

- Fluorescence Proteins -iRFP: miRFPnano670 -RFPs: mRuby3, mCherry -GFPs: mGreenLantern, EGFP -BFPs: TagBFP2, TagBFP -Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1 -Liver specific: -Brain endothelial cell specific: AAV-BI30 -Tissue/Cell type specific promoter Pan-neuron: hSyn -Kügler, S., E. Kilic, and M. Bähr. “Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. +
+

Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. — Sydney Brenner

+

diff --git a/public/index.xml b/public/index.xml index f48e0eb..0b48600 100644 --- a/public/index.xml +++ b/public/index.xml @@ -23,7 +23,9 @@ Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC Thu, 25 Aug 2022 17:16:39 +0800 https://leoj.xyz/resource/biochemistrytools/ - Vendor companies Abcam ThermoFisher R&amp;D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange + <blockquote> +<p>Our cells engage in protein production, and many of those proteins are enzymes responsible for the chemistry of life. &mdash; Randy Schekman</p> +</blockquote> @@ -32,15 +34,9 @@ Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC Sun, 21 Aug 2022 20:27:51 +0800 https://leoj.xyz/resource/molecularbiology/ - Fluorescence Proteins -iRFP: miRFPnano670 -RFPs: mRuby3, mCherry -GFPs: mGreenLantern, EGFP -BFPs: TagBFP2, TagBFP -Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1 -Liver specific: -Brain endothelial cell specific: AAV-BI30 -Tissue/Cell type specific promoter Pan-neuron: hSyn -Kügler, S., E. Kilic, and M. Bähr. &ldquo;Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. + <blockquote> +<p>Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. &mdash; Sydney Brenner</p> +</blockquote> @@ -146,9 +142,8 @@ Samuel Lundt#, Nannan Zhang#, Jun-Liszt Li, Zhe Zhang, Xiaowan Wang, Ruisi Bao, Mon, 15 Aug 2022 15:15:42 +0800 https://leoj.xyz/page/about/ - Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. I ask questions in the cutting-edge field of brain vasculature system and brain metabolic system, working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, and next-generation microscopy (Lightsheet, Confocal, 2p), in order to understand the fundamental basis of brain metabolism and brain vasculature system. -Interest Areas Biological Question -Brain vasculature system + Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. My long-term goal is to discover and characterize basic mechanisms underlying brain aging and neuron growth. In addition, I working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, next-generation microscopy (Lightsheet, Confocal, 2p), and next-generation gene therapy (AAV, promoter) in order to apply these technologies to solve specific problems. +Interest Areas Biological Question diff --git a/public/page/about/index.html b/public/page/about/index.html index 5de3f92..af06ad3 100644 --- a/public/page/about/index.html +++ b/public/page/about/index.html @@ -16,7 +16,7 @@ }, "name":"Jun-Liszt Li", - "description":"Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. I ask questions in the cutting-edge field of brain vasculature system and brain metabolic system, working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, and next-generation microscopy (Lightsheet, Confocal, 2p), in order to understand the fundamental basis of brain metabolism and brain vasculature system.\nInterest Areas Biological Question\nBrain vasculature system", + "description":"Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. My long-term goal is to discover and characterize basic mechanisms underlying brain aging and neuron growth. In addition, I working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, next-generation microscopy (Lightsheet, Confocal, 2p), and next-generation gene therapy (AAV, promoter) in order to apply these technologies to solve specific problems.\nInterest Areas Biological Question", "url":"https:\/\/leoj.xyz\/page\/about\/", "keywords":"[tech]" } @@ -28,26 +28,22 @@ - + - + - - + + @@ -337,7 +333,7 @@

leo1erefdfxc

-

Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. I ask questions in the cutting-edge field of brain vasculature system and brain metabolic system, working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, and next-generation microscopy (Lightsheet, Confocal, 2p), in order to understand the fundamental basis of brain metabolism and brain vasculature system.

+

Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. My long-term goal is to discover and characterize basic mechanisms underlying brain aging and neuron growth. In addition, I working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, next-generation microscopy (Lightsheet, Confocal, 2p), and next-generation gene therapy (AAV, promoter) in order to apply these technologies to solve specific problems.

Interest Areas

  • @@ -416,7 +412,7 @@

    Research Experiences

    HZAU-iGEM team member, Inovation Lab, College of Life science and technology, HZAU
    FEBRUARY 2015 - OCTOBER 2016
    -2016 HZAU-iGEM team member with instructor Dr.BingGuang Ma, PhD

    +2016 HZAU-iGEM team member with instructor Dr.Bin-Guang Ma, PhD

    • Systems and Synthetic Biology learning: student presentation, case-based learning, and discussion
    • During the Competition and in our project, I am responsible for the Optical control part which uses light switchable circuit (LSC) to control the expression of bacteria movement related gene CheZ.
      • diff --git a/public/page/index.html b/public/page/index.html index c4ab53c..f7573da 100644 --- a/public/page/index.html +++ b/public/page/index.html @@ -409,9 +409,8 @@

- Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. I ask questions in the cutting-edge field of brain vasculature system and brain metabolic system, working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, and next-generation microscopy (Lightsheet, Confocal, 2p), in order to understand the fundamental basis of brain metabolism and brain vasculature system. + Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. My long-term goal is to discover and characterize basic mechanisms underlying brain aging and neuron growth. In addition, I working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, next-generation microscopy (Lightsheet, Confocal, 2p), and next-generation gene therapy (AAV, promoter) in order to apply these technologies to solve specific problems. Interest Areas Biological Question -Brain vasculature system

diff --git a/public/page/index.xml b/public/page/index.xml index a3e42f8..45f65d9 100644 --- a/public/page/index.xml +++ b/public/page/index.xml @@ -35,9 +35,8 @@ Samuel Lundt#, Nannan Zhang#, Jun-Liszt Li, Zhe Zhang, Xiaowan Wang, Ruisi Bao, Mon, 15 Aug 2022 15:15:42 +0800 https://leoj.xyz/page/about/ - Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. I ask questions in the cutting-edge field of brain vasculature system and brain metabolic system, working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, and next-generation microscopy (Lightsheet, Confocal, 2p), in order to understand the fundamental basis of brain metabolism and brain vasculature system. -Interest Areas Biological Question -Brain vasculature system + Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. My long-term goal is to discover and characterize basic mechanisms underlying brain aging and neuron growth. In addition, I working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, next-generation microscopy (Lightsheet, Confocal, 2p), and next-generation gene therapy (AAV, promoter) in order to apply these technologies to solve specific problems. +Interest Areas Biological Question diff --git a/public/page/publication/index.html b/public/page/publication/index.html index 1051d4d..3b50dcb 100644 --- a/public/page/publication/index.html +++ b/public/page/publication/index.html @@ -356,7 +356,7 @@

Journal Publications

Gao X#, Li JL, Chen X, Ci B, Chen F, Lu N, Shen B, Zheng L, Jia J, Yi Y, Zhang S, Shi Y, Shi K, Propson NE, Huang Y, Poinsatte K, Zhang Z, Yue Y, Bosco DB, Lu YM, Yang SB, Adams RF, Lindner V, Huang F, Wu LJ, Zheng H, Han F, Hippenmeyer S, Stowe AM, Peng B, Margeta M, Wang X, Liu Q, Korbelin J, Trepel M, Lu H, Zhou BO, Zhao H, Sun W, Bachoo RM, Ge WP✝ (9/15/2020) Reduction of neuronal activity mediated by blood-vessel regression in the brain. BioRxiv [PDF | Link] Second author

  • -

    Wentong Hong# , Zhonggan Ren# , Pifang Gong# , Jun-Liszt Li. Wenzhi Sun, Woo-PingGe, Chun-Li Zhang, Shumin Duan, Song Qin✝ (2020) Temporal-spatial generation of astrocytes in the developing diencephalon; J. Neurosci, under review; Second author

    +

    Wentong Hong# , Zhonggan Ren# , Pifang Gong# , Jun-Liszt Li. Wenzhi Sun, Woo-Ping Ge, Chun-Li Zhang, Shumin Duan, Song Qin✝ (2020) Temporal-spatial generation of astrocytes in the developing diencephalon; J. Neurosci, under review; Second author

  • Beal J, Haddock-Angelli T, Gershater M, de Mora K, Lizarazo M, Hollenhorst J, Randy Rettberg✝,iGEM interlab Study Contributors (2016) Reproducibility of Fluorescent Expression from Engineered Biological Constructs in E. coli. PLoS 7NE 11(3): e0150182. doiV10.13•1vjournal.pone.0150182 2015 iGEM Interlab Study Contributors: HZAU-China: Pan Chu, Jun Li(Jun-Liszt Li), Keji Yan [PDF | DOI Link]

    diff --git a/public/resource/biochemistrytools/index.html b/public/resource/biochemistrytools/index.html index 6e19cde..d13e807 100644 --- a/public/resource/biochemistrytools/index.html +++ b/public/resource/biochemistrytools/index.html @@ -16,7 +16,7 @@ }, "name":"Jun-Liszt Li", - "description":"Vendor companies Abcam ThermoFisher R\u0026amp;D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange ", + "description":"\u003cblockquote\u003e\n\u003cp\u003eOur cells engage in protein production, and many of those proteins are enzymes responsible for the chemistry of life. \u0026mdash; Randy Schekman\u003c\/p\u003e\n\u003c\/blockquote\u003e", "url":"https:\/\/leoj.xyz\/resource\/biochemistrytools\/", "keywords":"[tech]" } @@ -28,18 +28,26 @@ - + - + - - + + @@ -58,11 +66,11 @@ - + - + - + @@ -331,35 +339,121 @@

    -

    Vendor companies

    +
    +

    Our cells engage in protein production, and many of those proteins are enzymes responsible for the chemistry of life. — Randy Schekman

    +
    +

    randyschekman1

    +
    +

    Foundational techniques

    +
      +
    • +

      Protein quantification
      + Western blot

      +
      • +
    • +

      Protein purification
      + Purifying Challenging Proteins, Principles and Methods [PDF]

      +
      • +
    +
    +

    Vendor companies

      +
    1. Cell Signaling Technology
    2. Abcam
    3. ThermoFisher
    4. R&D Systems
    5. BioRad
    -

    Ref Labs

    +
    +

    Ref. Labs

    +

    Signaling pathway and basic science

    +
      +
    1. Zhijian-James Chen lab, UTSW
      + Discovery of cGAS-cGAMP pathway and its role in immune defense
      2. +
    +
      +
    • Sun, Lijun, et al. “Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway.” Science 339.6121 (2013): 786-791. [PDF | PMID: 23258413 | DOI Link]
      • +
    • Wu, Jiaxi, et al. “Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA.” Science 339.6121 (2013): 826-830. [PDF | DOI Link]
      • +
    +
      +
    1. David M. Sabatini lab, WIBR, MIT
      + The discovery of mTOR
      2. +
    +
      +
    • Sabatini et al. “RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs.” Cell 78.1 (1994): 35-43. [PDF | DOI Link]
      + Systematic characterzation of basic mechanisms that regulate growth including nutrient sensing pathways [CASTOR | Sestrin2 | SAMTOR]
      • +
    +
      +
    1. Xiaodong Wang lab, UTSW, NIBS
      + The discovery of mitochondria CytoC mediated apoptosis pathway
      2. +
    +
      +
    • Zou, Hua, et al. “Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c–dependent activation of caspase-3.” Cell 90.3 (1997): 405-413. +[PDF | PMID: 9267021 | DOI Link]
      • +
    +
      +
    1. Feng Shao lab, NIBS
      + The discovery of GSDMD mediated pyroptosis pathway
      2. +
      -
    • Signaling pathway and basic science
      • +
    • Shi, Jianjin, et al. “Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.” Nature 526.7575 (2015): 660-665. [PDF | DOI Link]
    +

    Transcriptional regulation and epigenetics

      -
    1. Zhijian-James Chen lab, UTSW
      2. -
    2. David M. Sabatini lab, MIT
      3. -
    3. Xiaodong Wang lab, UTSW, NIBS
      4. -
    4. Feng Shao lab, NIBS
      5. +
    5. Robert Tjian, University of California, Berkeley/HHMI
      + The discovery of the SV40 large T antigen
      6. +
    +
      +
    • Tjian, Robert. “The binding site on SV40 DNA for a T antigen-related protein.” Cell 13.1 (1978): 165-179. [PMID: 202398 | DOI Link]
      • +
    +
      +
    1. +

      Yi Zhang lab, University of North Carolina at Chapel Hill/HMS/HHMI

      +
      2. +
    2. +

      Yang Shi lab, HMS/HHMI/University of Oxford
      + The discovery of first histone demethylase, LSD1

      +
      -
    • Tool development
      • +
    • Shi, Yujiang, et al. “Histone demethylation mediated by the nuclear amine oxidase homolog LSD1.” Cell 119.7 (2004): 941-953. [PDF | DOI Link]
    +
      +
    1. Chuan He lab, University of Chicago/HHMI
      + The discovery of first RNA demethylase that oxidatively reverses N6-methyladenosine (m6A) methylation in mammalian messenger RNA
      2. +
    +
      +
    • Jia, Guifang, et al. “N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO.” Nature chemical biology 7.12 (2011): 885-887. [PDF | DOI Link]
      • +
    +

    Tool development

    1. Alice Ting lab, Stanford
    +

    Ref. Protocols

    1. Bio-protocol
    2. Protocol Exchange
    - +
    +

    Nobel laureates

    +
      +
    1. +

      Randy Schekman, University of California, Berkeley
      +The Nobel Prize in Physiology or Medicine 2013
      +Genes and Proteins That Control the Secretory Pathway [Nobel Lecture video | Lecture Slides | Read the Lecture | Source]

      +
      2. +
    2. +

      Thomas C. Südhof, Stanford University
      +The Nobel Prize in Physiology or Medicine 2013
      +The Molecular Machine of Neurotransmitter Release [Nobel Lecture video | Lecture Slides | Read the Lecture | Source]

      +
      3. +
    3. +

      Yoshinori Ohsumi, Tokyo Institute of Technology, Tokyo, Japan
      +The Nobel Prize in Physiology or Medicine 2016
      +Molecular Mechanisms of Autophagy in Yeast [Nobel Lecture video | Lecture Slides | Read the Lecture | Source]

      +
      4. +
    @@ -373,11 +467,11 @@

    Ref. Protocols

    TAGGED IN
    - PEGASOS + Cell biology - CUBIC + Metabolism - Clarity + Cell signaling
    diff --git a/public/resource/geneticstools/index.html b/public/resource/geneticstools/index.html index 71da493..09d7b12 100644 --- a/public/resource/geneticstools/index.html +++ b/public/resource/geneticstools/index.html @@ -62,11 +62,13 @@ - + - + - + + + @@ -340,7 +342,7 @@

    Mus musculus

    mus129s1 129S1 c57bl6jx1 C57BL/6J balbcjx1 BALB/cJ

    -

    Mouse genetics tools in brain vasculature system field

    +

    Mouse genetics tools in brain vasculature system field

    1. NG2DsRedBAC: NG2DsRedBAC transgenic mice is suitable for pericyte and Artery SMC labeling in central nervous system. NG2DsRedBAC transgenic mice express an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer and may be useful for fluorescent labeling of NG2 cells (oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs, as well as for isolating NG2 cell populations via FACS. [JAX Strain #:008241 | Genotyping Protocol] | DOI Link]

      @@ -349,6 +351,9 @@

      Mouse genetics t

      Tek-Cre: The Tie2-Cre (Tek-Cre) transgene has the mouse endothelial-specific receptor tyrosine kinase (Tek or Tie2) promoter directing expression of Cre recombinase. These Tek-Cre transgenic mice are a Cre-lox tool useful for deletion of floxed sequences in endothelial cells during embryogenesis and adulthood. Cre recombinase activity is also detected in microglia in central nervous system. [JAX Strain #:008863 | Genotyping Protocol | DOI Link]

    2. +

      Cdh5-PAC-CreERT2: [Genotyping Protocol | DOI Link]

      +
      3. +

    3. Pdgfrb-cre: Pericyte labeling. DOI Link

    4. @@ -362,15 +367,29 @@

      Mouse genetics t

    -

    Astrocyte labeling

    +

    Astrocyte labeling

      -
    1. Aldh1l1-EGFP
      2. -
    2. Aldh1l1-CreERT2
      3. -
    3. mGFAP-Cre
      4. -
    4. hGFAP-Cre: -5.
      5. +
    5. +

      Aldh1l1-EGFP

      +
      6. +
    6. +

      Aldh1l1-CreERT2

      +
      7. +
    7. +

      mGFAP-Cre

      +
      8. +
    8. +

      hGfap-CreERT2:
      +Park, Yongmin Mason, et al. “Astrocyte specificity and coverage of hGFAP-CreERT2 [Tg (GFAP-Cre/ERT2) 13Kdmc] mouse line in various brain regions.” Experimental Neurobiology 27.6 (2018): 508. [PMID: 30636902 | DOI Link]

      +
      9. +
    9. +

      Glast-CreERT2

      +
      10. +
    10. +

      Fgfr3-iCreERT2

      +
    -

    Neuron labeling

    +

    Neuron labeling

    1. Thy1-YFP-M: These Thy1-GFP-M transgenic mice may be useful in neurobiological studies for fluorescent labeling of neural tissues, especially for mossy fibers in the cerebellum and intense, yet sparse, labeling of a variety of neuronal subsets. [JAX Strain #:007788 | Genotyping Protocol]

      @@ -396,23 +415,47 @@

      Neuron labeling

    2. Vglut1-Cre: Excitatory neuron labeling.

      3. -
    3. +
    4. +

      CamKII-Cre: Expression pattern in brain(Throughout the cortex, hippocampus, striatum, and other structures) | [Susumu Tonegawa]

      +
    -

    Microglia labeling

    +

    Microglia labeling

    1. CX3CR1-EGFP: [JAX Strain #:005582 | Genotyping Protocol]
    2. CX3CR1-iCreERT2:
    3. Tmem119-P2A-iCreERT2: [JAX Strain #:031820 | Genotyping Protocol | DOI Link]
    -
    +

    Oligodendrocytes

    +
      +
    1. Oligo2-Cre
      2. +
    +

    Reporter Lines

    +
      +
    1. Ai14(Rosa26-CAG-LSL-tdTomato)
      2. +
    2. Ai47(Rosa26-CAG-LSL-3x-GFP)
      3. +
    3. Ai140(TRE-)
      4. +
    4. +
    +

    Transgenic line for Calcium imaging

    +
      +
    1. Thy1-GCaMP6f
      2. +
    2. Thy1-JRGECO1a
      3. +
    3. Ai96(Rosa26-CAG-LSL-GCaMP6s)
      4. +
    +

    Transgenic line for genome editing

    +
      +
    1. Rosa26-CAG-LSL-SpCas9-P2A-EGFP
      2. +
    2. Rosa26-CAG-SpCas9-P2A-EGFP
      3. +

    Ref. public websites

    1. The Jackson Laborary: The laboratory is also the world’s source for more than 8,000 strains of genetically defined mice, and serves as a worldwide center for scientific courses, conferences, training, and education.
    2. Mouse Genome Informatics: MGI is the international database resource for the laboratory mouse, providing integrated genetic, genomic, and biological data to facilitate the study of human health and disease.
    -

    Ref Labs

      +
    1. Allen Institute for Brain Research:
      2. +
    2. Susumu Tonegawa Lab, MIT
    3. Masashi Yanagisawa Lab, The International Institute for Integrative Sleep(IIIS)
    4. Z. Josh Huang Lab, Duke University
    @@ -420,8 +463,38 @@

    Ref Labs

    Rat

    Drosophila melanogaster

    +

    seymour_benzer1 +Seymour Benzer with mega-Drosophila, 1974.

    +

    Ref. Websites

    +
      +
    1. FlyBase: A Database of Drosophila Genes & Genomes
      2. +

    Zebrafish

    +

    Ref. Websites

    +
      +
    1. ZFIN: The Zebrafish Information Network (ZFIN) is the database of genetic and genomic data for the zebrafish (Danio rerio) as a model organism. ZFIN provides a wide array of expertly curated, organized and cross-referenced zebrafish research data.
      2. +
    +
    +

    Nobel laureates

    + @@ -436,11 +509,13 @@

    Zebrafish

    TAGGED IN
    - PEGASOS + Gentics + + Mouse - CUBIC + Drosophila - Clarity + Zebrafish
    diff --git a/public/resource/genomicsngs/index.html b/public/resource/genomicsngs/index.html index cd24b9c..db9965d 100644 --- a/public/resource/genomicsngs/index.html +++ b/public/resource/genomicsngs/index.html @@ -422,6 +422,21 @@

    Ref labs

    Single cell genomics: Satija lab, NYU
    Spatial Transcriptomics: Rongfan lab, Yale

    +

    Nobel laureates

    +
      +
    1. +

      Frederick Sanger(1918-2013), Cambridge University, Great Britain
      +The Nobel Prize in Chemistry 1980
      +Determination of Nucleotide Sequences in DNA +[Public talk | Read the Lecture | Source]

      +
      2. +
    2. +

      Walter Gilbert(1932-), Harvard University, Biological Laboratories, Cambridge, MA, USA
      +The Nobel Prize in Chemistry 1980
      +DNA Sequencing and Gene Structure +[iBiology interview | Read the Lecture | Source]

      +
      3. +
    diff --git a/public/resource/index.html b/public/resource/index.html index 75fe0c4..4faf003 100644 --- a/public/resource/index.html +++ b/public/resource/index.html @@ -363,7 +363,9 @@

    - Vendor companies Abcam ThermoFisher R&D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange +
    +

    Our cells engage in protein production, and many of those proteins are enzymes responsible for the chemistry of life. — Randy Schekman

    +

    @@ -406,15 +408,9 @@

    - Fluorescence Proteins -iRFP: miRFPnano670 -RFPs: mRuby3, mCherry -GFPs: mGreenLantern, EGFP -BFPs: TagBFP2, TagBFP -Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1 -Liver specific: -Brain endothelial cell specific: AAV-BI30 -Tissue/Cell type specific promoter Pan-neuron: hSyn -Kügler, S., E. Kilic, and M. Bähr. “Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. +
    +

    Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. — Sydney Brenner

    +

    diff --git a/public/resource/index.xml b/public/resource/index.xml index 146c71b..637aa1f 100644 --- a/public/resource/index.xml +++ b/public/resource/index.xml @@ -23,7 +23,9 @@ Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC Thu, 25 Aug 2022 17:16:39 +0800 https://leoj.xyz/resource/biochemistrytools/ - Vendor companies Abcam ThermoFisher R&amp;D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange + <blockquote> +<p>Our cells engage in protein production, and many of those proteins are enzymes responsible for the chemistry of life. &mdash; Randy Schekman</p> +</blockquote> @@ -32,15 +34,9 @@ Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC Sun, 21 Aug 2022 20:27:51 +0800 https://leoj.xyz/resource/molecularbiology/ - Fluorescence Proteins -iRFP: miRFPnano670 -RFPs: mRuby3, mCherry -GFPs: mGreenLantern, EGFP -BFPs: TagBFP2, TagBFP -Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1 -Liver specific: -Brain endothelial cell specific: AAV-BI30 -Tissue/Cell type specific promoter Pan-neuron: hSyn -Kügler, S., E. Kilic, and M. Bähr. &ldquo;Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. + <blockquote> +<p>Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. &mdash; Sydney Brenner</p> +</blockquote> diff --git a/public/resource/molecularbiology/index.html b/public/resource/molecularbiology/index.html index 3c63eba..e4233f7 100644 --- a/public/resource/molecularbiology/index.html +++ b/public/resource/molecularbiology/index.html @@ -16,7 +16,7 @@ }, "name":"Jun-Liszt Li", - "description":"Fluorescence Proteins -iRFP: miRFPnano670\nRFPs: mRuby3, mCherry\nGFPs: mGreenLantern, EGFP\nBFPs: TagBFP2, TagBFP\nReference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue\/Cell type specific AAVs Brain organ specific: BR1\nLiver specific:\nBrain endothelial cell specific: AAV-BI30\nTissue\/Cell type specific promoter Pan-neuron: hSyn\nKügler, S., E. Kilic, and M. Bähr. \u0026ldquo;Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area.", + "description":"\u003cblockquote\u003e\n\u003cp\u003eProgress in science depends on new techniques, new discoveries and new ideas, probably in that order. \u0026mdash; Sydney Brenner\u003c\/p\u003e\n\u003c\/blockquote\u003e", "url":"https:\/\/leoj.xyz\/resource\/molecularbiology\/", "keywords":"[tech]" } @@ -28,50 +28,26 @@ - - - - + + + + - - + + @@ -363,31 +339,17 @@

    -

    Fluorescence Proteins

    -

    -iRFP: miRFPnano670

    -
      -
    • -

      RFPs: mRuby3, mCherry

      -
      • -
    • -

      GFPs: mGreenLantern, EGFP

      -
      • -
    • -

      BFPs: TagBFP2, TagBFP

      -
      • -
    -
    -

    Reference

    -
      -
    1. FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists.
      2. -
    +
    +

    Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. — Sydney Brenner

    +
    +

    sydneybrenner1

    Tissue/Cell type specific AAVs

    -

    Brain organ specific: BR1
    +

    Brain organ specific: AAV-BR1[PDF | PMID: | DOI Link]
    Liver specific:

    -

    Brain endothelial cell specific: AAV-BI30

    +

    Brain endothelial cell specific: AAV-BI30[PDF | PMID: | DOI Link]

    Tissue/Cell type specific promoter

    @@ -401,6 +363,24 @@

    Tissue/Cell type specific promoterConstitutive expression: EF1a, CAG

    Drug induced: Tet-on system

    Activity depedent: c-Fos promoter

    +
    +

    Fluorescence Proteins

    +

    -iRFP: miRFPnano670

    +
      +
    • +

      RFPs: mRuby3, mCherry

      +
      • +
    • +

      GFPs: mGreenLantern, EGFP

      +
      • +
    • +

      BFPs: TagBFP2, TagBFP

      +
      • +
    +

    Ref. websites

    +
      +
    1. FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists.
      2. +

    Genetically encoded indicators

    DNA base editor

    +

    Optogenetics tools

    • @@ -446,7 +427,7 @@

      Optogenetics tools

      Light-activated Cyclases: PAC & Cyclop

    -

    Reference

    +

    Reference

    1. Resource website of Optogenetics in neuroscience, Karl Deisseroth lab
    2. Georg Nagel lab, University of Würzburg
      3. @@ -466,11 +447,85 @@

      Vendor company

      Ref Labs

      1. Feng Zhang lab, MIT
        2. +
      2. David Liu lab, Harvard
      3. Karl Deisseroth lab, Stanford
      4. Yulong Li lab, PKU
        5. -
      5. David Liu lab, Harvard
        6. +
      6. Jin Zhang lab, UCSD
      - +
      +

      Nobel laureates

      +
        +
      • +

        Emmanuelle Charpentier, Max Planck Unit for the Science of Pathogens, Berlin, Germany
        +The Nobel Prize in Chemistry 2020
        +For the development of a method for genome editing [Nobel Lecture video]

        +
        • +
      • +

        Jennifer A. Doudna, University of California, Berkeley, CA, USA
        +The Nobel Prize in Chemistry 2020
        +The Chemistry of CRISPR: Editing the Code of Life [Nobel Lecture video]

        +
        • +
      • +

        Osamu Shimomura, Marine Biological Laboratory (MBL), Woods Hole, MA, USA; Boston University Medical School, Massachusetts, MA, USA
        +The Nobel Prize in Chemistry 2008
        +Discovery of Green Fluorescent Protein, GFP [Nobel Lecture video | Lecture Slides | Read the Lecture | Source]

        +
        • +
      • +

        Martin Chalfie, Columbia University, New York, NY, USA
        +The Nobel Prize in Chemistry 2008
        +GFP: Lighting Up Life [Nobel Lecture video | Lecture Slides | Source]

        +
        • +
      • +

        Roger Y. Tsien, University of California, San Diego, CA, USA
        +The Nobel Prize in Chemistry 2008
        +Constructing and Exploiting the Fluorescent Protein Paintbox [Nobel Lecture video | Lecture Slides | Read the Lecture | Source]

        +
        • +
      • +

        Craig C. Mello, University of Massachusetts Medical School, Worcester, MA, USA
        +The Nobel Prize in Physiology or Medicine 2006
        +Return to the RNAi World: Rethinking Gene Expression and Evolution [Nobel Lecture video | Lecture Slides | Read the Lecture | Source]

        +
        • +
      • +

        Andrew Z. Fire, Stanford University School of Medicine, Stanford, CA, USA
        +The Nobel Prize in Physiology or Medicine 2006
        +Gene Silencing by Double Stranded RNA [Nobel Lecture video | Lecture Slides | Read the Lecture | Source]

        +
        • +
      • +

        Phillip A. Sharp, Massachusetts Institute of Technology (MIT), Center for Cancer Research, Cambridge, MA, USA
        +The Nobel Prize in Physiology or Medicine 1993
        +Split Genes and RNA Splicing [Nobel Lecture video | Read the Lecture | Source]

        +
        • +
      • +

        Richard Roberts, New England Biolabs, Beverly, MA, USA
        +The Nobel Prize in Physiology or Medicine 1993
        +An Amazing Distortion in DNA Induced by a Methyltransferase [Nobel Lecture video | Read the Lecture | Source]

        +
        • +
      • +

        Susumu Tonegawa, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA
        +The Nobel Prize in Physiology or Medicine 1987
        +Somatic Generation of Immune Diversity [[Nobel Lecture video | Read the Lecture | Source]

        +
        • +
      • +

        Paul Berg, Stanford University, Stanford, CA, USA
        +The Nobel Prize in Chemistry 1980
        +Dissections and Reconstructions of Genes and Chromosomes [Read the Lecture | Source]

        +
        • +
      • +

        Daniel Nathans, Johns Hopkins University School of Medicine, Baltimore, MD, USA
        +The Nobel Prize in Physiology or Medicine 1978
        +Restriction Endonucleases, Simian Virus 40, and the New Genetics [Read the Lecture | Source]

        +
        • +
      • +

        Werner Arber, Biozentrum der Universität, Basel, Switzerland
        +The Nobel Prize in Physiology or Medicine 1978
        +Promotion and Limitation of Genetic Exchange [Read the Lecture | Source]

        +
        • +
      • +

        Hamilton O. Smith, Johns Hopkins University School of Medicine, Baltimore, MD, USA
        +The Nobel Prize in Physiology or Medicine 1978
        +Nucleotide Sequence Specificity of Restriction Endonucleases [Read the Lecture | Source]

        +
        • +
      diff --git a/public/sitemap.xml b/public/sitemap.xml index 7c511cb..731685f 100644 --- a/public/sitemap.xml +++ b/public/sitemap.xml @@ -8,19 +8,19 @@ https://leoj.xyz/categories/category/ 2022-08-26T16:50:25+08:00 - https://leoj.xyz/tags/clarity/ + https://leoj.xyz/tags/drosophila/ 2022-08-26T16:50:25+08:00 - https://leoj.xyz/tags/cubic/ + https://leoj.xyz/resource/geneticstools/ 2022-08-26T16:50:25+08:00 - https://leoj.xyz/resource/geneticstools/ + https://leoj.xyz/tags/gentics/ 2022-08-26T16:50:25+08:00 https://leoj.xyz/ 2022-08-26T16:50:25+08:00 - https://leoj.xyz/tags/pegasos/ + https://leoj.xyz/tags/mouse/ 2022-08-26T16:50:25+08:00 https://leoj.xyz/resource/ @@ -31,9 +31,21 @@ https://leoj.xyz/tags/ 2022-08-26T16:50:25+08:00 + + https://leoj.xyz/tags/zebrafish/ + 2022-08-26T16:50:25+08:00 https://leoj.xyz/resource/biochemistrytools/ 2022-08-25T17:16:39+08:00 + + https://leoj.xyz/tags/cell-biology/ + 2022-08-25T17:16:39+08:00 + + https://leoj.xyz/tags/cell-signaling/ + 2022-08-25T17:16:39+08:00 + + https://leoj.xyz/tags/metabolism/ + 2022-08-25T17:16:39+08:00 https://leoj.xyz/tags/crispr/ 2022-08-21T20:27:51+08:00 @@ -58,6 +70,15 @@ https://leoj.xyz/tags/orbitrap/ 2022-08-21T13:24:59+08:00 + + https://leoj.xyz/tags/clarity/ + 2022-08-21T12:23:57+08:00 + + https://leoj.xyz/tags/cubic/ + 2022-08-21T12:23:57+08:00 + + https://leoj.xyz/tags/pegasos/ + 2022-08-21T12:23:57+08:00 https://leoj.xyz/resource/clearingmethod/ 2022-08-21T12:23:57+08:00 diff --git a/public/tags/cell-biology/index.html b/public/tags/cell-biology/index.html new file mode 100644 index 0000000..0d50e02 --- /dev/null +++ b/public/tags/cell-biology/index.html @@ -0,0 +1,468 @@ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + Cell biology + + + + + + + + + + + + + + + + + + + + + + + + + + + +
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      Jun-Liszt Li

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      Hello, My name is Jun-Liszt Li. my science interest is to understand the fundamental basis of brain metabolic system and brain vasculature system.
      + + +
      + +
      + Ph.D. Candidate +
      + + +
      + +
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      Hello, My name is Jun-Liszt Li. my science inter -
      -
      - -
      -

      - - - - Genetics tools - -

      - -
      -
      - - Mus musculus 129S1 C57BL/6J BALB/cJ -Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC transgenic mice is suitable for pericyte and Artery SMC labeling in central nervous system. NG2DsRedBAC transgenic mice express an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer and may be useful for fluorescent labeling of NG2 cells (oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs, as well as for isolating NG2 cell populations via FACS. - -

      - - Continue reading - - -

      -
      -
      - - -
      - -
      -       - -
      - - - - - - - - - - -
      -
      - -
      -

      - - - - Biochemistry tools - -

      - -
      -
      - - Vendor companies Abcam ThermoFisher R&D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange - -

      - - Continue reading - - -

      -
      -
      - - -
      - -
      -       - -
      - - - - - - - - - -
      diff --git a/public/tags/clarity/index.xml b/public/tags/clarity/index.xml index 9146ba3..732b9ac 100644 --- a/public/tags/clarity/index.xml +++ b/public/tags/clarity/index.xml @@ -1,31 +1,12 @@ - Clarity on Jun-Liszt Li + ClARITY on Jun-Liszt Li https://leoj.xyz/tags/clarity/ - Recent content in Clarity on Jun-Liszt Li + Recent content in ClARITY on Jun-Liszt Li Hugo -- gohugo.io en-us - Fri, 26 Aug 2022 16:50:25 +0800 - - Genetics tools - https://leoj.xyz/resource/geneticstools/ - Fri, 26 Aug 2022 16:50:25 +0800 - - https://leoj.xyz/resource/geneticstools/ - Mus musculus 129S1 C57BL/6J BALB/cJ -Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC transgenic mice is suitable for pericyte and Artery SMC labeling in central nervous system. NG2DsRedBAC transgenic mice express an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer and may be useful for fluorescent labeling of NG2 cells (oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs, as well as for isolating NG2 cell populations via FACS. - - - - Biochemistry tools - https://leoj.xyz/resource/biochemistrytools/ - Thu, 25 Aug 2022 17:16:39 +0800 - - https://leoj.xyz/resource/biochemistrytools/ - Vendor companies Abcam ThermoFisher R&amp;D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange - - + Sun, 21 Aug 2022 12:23:57 +0800 Tissue clearing methods https://leoj.xyz/resource/clearingmethod/ diff --git a/public/tags/crispr/index.html b/public/tags/crispr/index.html index c2e0e82..c3fc350 100644 --- a/public/tags/crispr/index.html +++ b/public/tags/crispr/index.html @@ -321,15 +321,9 @@

      - Fluorescence Proteins -iRFP: miRFPnano670 -RFPs: mRuby3, mCherry -GFPs: mGreenLantern, EGFP -BFPs: TagBFP2, TagBFP -Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1 -Liver specific: -Brain endothelial cell specific: AAV-BI30 -Tissue/Cell type specific promoter Pan-neuron: hSyn -Kügler, S., E. Kilic, and M. Bähr. “Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. +
      +

      Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. — Sydney Brenner

      +

      diff --git a/public/tags/crispr/index.xml b/public/tags/crispr/index.xml index 3f46c48..fffbea9 100644 --- a/public/tags/crispr/index.xml +++ b/public/tags/crispr/index.xml @@ -13,15 +13,9 @@ Sun, 21 Aug 2022 20:27:51 +0800 https://leoj.xyz/resource/molecularbiology/ - Fluorescence Proteins -iRFP: miRFPnano670 -RFPs: mRuby3, mCherry -GFPs: mGreenLantern, EGFP -BFPs: TagBFP2, TagBFP -Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1 -Liver specific: -Brain endothelial cell specific: AAV-BI30 -Tissue/Cell type specific promoter Pan-neuron: hSyn -Kügler, S., E. Kilic, and M. Bähr. &ldquo;Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. + <blockquote> +<p>Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. &mdash; Sydney Brenner</p> +</blockquote> diff --git a/public/tags/cubic/index.html b/public/tags/cubic/index.html index 6a2e309..61c1b9d 100644 --- a/public/tags/cubic/index.html +++ b/public/tags/cubic/index.html @@ -306,93 +306,6 @@

      Hello, My name is Jun-Liszt Li. my science inter -
      -
      - -
      -

      - - - - Genetics tools - -

      - -
      -
      - - Mus musculus 129S1 C57BL/6J BALB/cJ -Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC transgenic mice is suitable for pericyte and Artery SMC labeling in central nervous system. NG2DsRedBAC transgenic mice express an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer and may be useful for fluorescent labeling of NG2 cells (oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs, as well as for isolating NG2 cell populations via FACS. - -

      - - Continue reading - - -

      -
      -
      - - -
      - -
      -       - -
      - - - - - - - - - - -
      -
      - -
      -

      - - - - Biochemistry tools - -

      - -
      -
      - - Vendor companies Abcam ThermoFisher R&D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange - -

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      Hello, My name is Jun-Liszt Li. my science interest is to understand the fundamental basis of brain metabolic system and brain vasculature system.
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      - Fluorescence Proteins -iRFP: miRFPnano670 -RFPs: mRuby3, mCherry -GFPs: mGreenLantern, EGFP -BFPs: TagBFP2, TagBFP -Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1 -Liver specific: -Brain endothelial cell specific: AAV-BI30 -Tissue/Cell type specific promoter Pan-neuron: hSyn -Kügler, S., E. Kilic, and M. Bähr. “Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. +
      +

      Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. — Sydney Brenner

      +

      diff --git a/public/tags/fp/index.xml b/public/tags/fp/index.xml index ea6fa30..36bac07 100644 --- a/public/tags/fp/index.xml +++ b/public/tags/fp/index.xml @@ -13,15 +13,9 @@ Sun, 21 Aug 2022 20:27:51 +0800 https://leoj.xyz/resource/molecularbiology/ - Fluorescence Proteins -iRFP: miRFPnano670 -RFPs: mRuby3, mCherry -GFPs: mGreenLantern, EGFP -BFPs: TagBFP2, TagBFP -Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1 -Liver specific: -Brain endothelial cell specific: AAV-BI30 -Tissue/Cell type specific promoter Pan-neuron: hSyn -Kügler, S., E. Kilic, and M. Bähr. &ldquo;Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. + <blockquote> +<p>Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. &mdash; Sydney Brenner</p> +</blockquote> diff --git a/public/tags/gentics/index.html b/public/tags/gentics/index.html new file mode 100644 index 0000000..dbebc7c --- /dev/null +++ b/public/tags/gentics/index.html @@ -0,0 +1,467 @@ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + Gentics + + + + + + + + + + + + + + + + + + + + + + + + + + + +

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      + + Mus musculus 129S1 C57BL/6J BALB/cJ +Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC transgenic mice is suitable for pericyte and Artery SMC labeling in central nervous system. NG2DsRedBAC transgenic mice express an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer and may be useful for fluorescent labeling of NG2 cells (oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs, as well as for isolating NG2 cell populations via FACS. + +

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      Jun-Liszt Li

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      Hello, My name is Jun-Liszt Li. my science interest is to understand the fundamental basis of brain metabolic system and brain vasculature system.
      + + +
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      + Ph.D. Candidate +
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      Hello, My name is Jun-Liszt Li. my science inter
      - - clarity - - - - cubic - - - - pegasos - - ggplot2 @@ -344,6 +332,14 @@
      Hello, My name is Jun-Liszt Li. my science inter brain-vasculature-system + + cell-biology + + + + cell-signaling + + cibr @@ -352,6 +348,10 @@
      Hello, My name is Jun-Liszt Li. my science inter cibr-talk + + clarity + + confocal @@ -364,6 +364,14 @@
      Hello, My name is Jun-Liszt Li. my science inter crispr + + cubic + + + + drosophila + + email @@ -372,6 +380,10 @@
      Hello, My name is Jun-Liszt Li. my science inter fp + + gentics + + hzau @@ -396,10 +408,18 @@
      Hello, My name is Jun-Liszt Li. my science inter maldi + + metabolism + + mini-review + + mouse + + ngs @@ -412,6 +432,10 @@
      Hello, My name is Jun-Liszt Li. my science inter paper + + pegasos + + pku @@ -460,123 +484,13 @@
      Hello, My name is Jun-Liszt Li. my science inter two-photon + + zebrafish + +
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      diff --git a/public/tags/index.xml b/public/tags/index.xml index de75337..4ac5c95 100644 --- a/public/tags/index.xml +++ b/public/tags/index.xml @@ -8,29 +8,65 @@ en-us Fri, 26 Aug 2022 16:50:25 +0800 - Clarity - https://leoj.xyz/tags/clarity/ + Drosophila + https://leoj.xyz/tags/drosophila/ Fri, 26 Aug 2022 16:50:25 +0800 - https://leoj.xyz/tags/clarity/ + https://leoj.xyz/tags/drosophila/ - CUBIC - https://leoj.xyz/tags/cubic/ + Gentics + https://leoj.xyz/tags/gentics/ Fri, 26 Aug 2022 16:50:25 +0800 - https://leoj.xyz/tags/cubic/ + https://leoj.xyz/tags/gentics/ - PEGASOS - https://leoj.xyz/tags/pegasos/ + Mouse + https://leoj.xyz/tags/mouse/ Fri, 26 Aug 2022 16:50:25 +0800 - https://leoj.xyz/tags/pegasos/ + https://leoj.xyz/tags/mouse/ + + + + + Zebrafish + https://leoj.xyz/tags/zebrafish/ + Fri, 26 Aug 2022 16:50:25 +0800 + + https://leoj.xyz/tags/zebrafish/ + + + + + Cell biology + https://leoj.xyz/tags/cell-biology/ + Thu, 25 Aug 2022 17:16:39 +0800 + + https://leoj.xyz/tags/cell-biology/ + + + + + Cell signaling + https://leoj.xyz/tags/cell-signaling/ + Thu, 25 Aug 2022 17:16:39 +0800 + + https://leoj.xyz/tags/cell-signaling/ + + + + + Metabolism + https://leoj.xyz/tags/metabolism/ + Thu, 25 Aug 2022 17:16:39 +0800 + + https://leoj.xyz/tags/metabolism/ @@ -88,6 +124,33 @@ + + ClARITY + https://leoj.xyz/tags/clarity/ + Sun, 21 Aug 2022 12:23:57 +0800 + + https://leoj.xyz/tags/clarity/ + + + + + CUBIC + https://leoj.xyz/tags/cubic/ + Sun, 21 Aug 2022 12:23:57 +0800 + + https://leoj.xyz/tags/cubic/ + + + + + PEGASOS + https://leoj.xyz/tags/pegasos/ + Sun, 21 Aug 2022 12:23:57 +0800 + + https://leoj.xyz/tags/pegasos/ + + + NGS https://leoj.xyz/tags/ngs/ diff --git a/public/tags/linux/index.html b/public/tags/linux/index.html index 03546d0..46ce23c 100644 --- a/public/tags/linux/index.html +++ b/public/tags/linux/index.html @@ -321,15 +321,9 @@

      - Fluorescence Proteins -iRFP: miRFPnano670 -RFPs: mRuby3, mCherry -GFPs: mGreenLantern, EGFP -BFPs: TagBFP2, TagBFP -Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1 -Liver specific: -Brain endothelial cell specific: AAV-BI30 -Tissue/Cell type specific promoter Pan-neuron: hSyn -Kügler, S., E. Kilic, and M. Bähr. “Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. +
      +

      Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. — Sydney Brenner

      +

      diff --git a/public/tags/linux/index.xml b/public/tags/linux/index.xml index 7d42f3a..3b9f59d 100644 --- a/public/tags/linux/index.xml +++ b/public/tags/linux/index.xml @@ -13,15 +13,9 @@ Sun, 21 Aug 2022 20:27:51 +0800 https://leoj.xyz/resource/molecularbiology/ - Fluorescence Proteins -iRFP: miRFPnano670 -RFPs: mRuby3, mCherry -GFPs: mGreenLantern, EGFP -BFPs: TagBFP2, TagBFP -Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1 -Liver specific: -Brain endothelial cell specific: AAV-BI30 -Tissue/Cell type specific promoter Pan-neuron: hSyn -Kügler, S., E. Kilic, and M. Bähr. &ldquo;Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. + <blockquote> +<p>Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. &mdash; Sydney Brenner</p> +</blockquote> diff --git a/public/tags/metabolism/index.html b/public/tags/metabolism/index.html new file mode 100644 index 0000000..0ee35fd --- /dev/null +++ b/public/tags/metabolism/index.html @@ -0,0 +1,468 @@ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + Metabolism + + + + + + + + + + + + + + + + + + + + + + + + + + + +

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      + + +
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      + + Mus musculus 129S1 C57BL/6J BALB/cJ +Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC transgenic mice is suitable for pericyte and Artery SMC labeling in central nervous system. NG2DsRedBAC transgenic mice express an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer and may be useful for fluorescent labeling of NG2 cells (oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs, as well as for isolating NG2 cell populations via FACS. + +

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      Hello, My name is Jun-Liszt Li. my science interest is to understand the fundamental basis of brain metabolic system and brain vasculature system.
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      Hello, My name is Jun-Liszt Li. my science inter -
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      - - Mus musculus 129S1 C57BL/6J BALB/cJ -Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC transgenic mice is suitable for pericyte and Artery SMC labeling in central nervous system. NG2DsRedBAC transgenic mice express an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer and may be useful for fluorescent labeling of NG2 cells (oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs, as well as for isolating NG2 cell populations via FACS. - -

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      diff --git a/public/tags/pegasos/index.xml b/public/tags/pegasos/index.xml index 0a64376..5002a38 100644 --- a/public/tags/pegasos/index.xml +++ b/public/tags/pegasos/index.xml @@ -6,26 +6,7 @@ Recent content in PEGASOS on Jun-Liszt Li Hugo -- gohugo.io en-us - Fri, 26 Aug 2022 16:50:25 +0800 - - Genetics tools - https://leoj.xyz/resource/geneticstools/ - Fri, 26 Aug 2022 16:50:25 +0800 - - https://leoj.xyz/resource/geneticstools/ - Mus musculus 129S1 C57BL/6J BALB/cJ -Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC transgenic mice is suitable for pericyte and Artery SMC labeling in central nervous system. NG2DsRedBAC transgenic mice express an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer and may be useful for fluorescent labeling of NG2 cells (oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs, as well as for isolating NG2 cell populations via FACS. - - - - Biochemistry tools - https://leoj.xyz/resource/biochemistrytools/ - Thu, 25 Aug 2022 17:16:39 +0800 - - https://leoj.xyz/resource/biochemistrytools/ - Vendor companies Abcam ThermoFisher R&amp;D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange - - + Sun, 21 Aug 2022 12:23:57 +0800 Tissue clearing methods https://leoj.xyz/resource/clearingmethod/ diff --git a/public/tags/pku/index.xml b/public/tags/pku/index.xml index 76d13b5..93da2cc 100644 --- a/public/tags/pku/index.xml +++ b/public/tags/pku/index.xml @@ -13,9 +13,8 @@ Mon, 15 Aug 2022 15:15:42 +0800 https://leoj.xyz/page/about/ - Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. I ask questions in the cutting-edge field of brain vasculature system and brain metabolic system, working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, and next-generation microscopy (Lightsheet, Confocal, 2p), in order to understand the fundamental basis of brain metabolism and brain vasculature system. -Interest Areas Biological Question -Brain vasculature system + Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. My long-term goal is to discover and characterize basic mechanisms underlying brain aging and neuron growth. In addition, I working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, next-generation microscopy (Lightsheet, Confocal, 2p), and next-generation gene therapy (AAV, promoter) in order to apply these technologies to solve specific problems. +Interest Areas Biological Question diff --git a/public/tags/zebrafish/index.html b/public/tags/zebrafish/index.html new file mode 100644 index 0000000..b11f4d8 --- /dev/null +++ b/public/tags/zebrafish/index.html @@ -0,0 +1,467 @@ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + Zebrafish + + + + + + + + + + + + + + + + + + + + + + + + + + + +
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      Hello, My name is Jun-Liszt Li. my science interest is to understand the fundamental basis of brain metabolic system and brain vasculature system.
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