From 15a430ddfa9292c2498592f832d888bad76022f8 Mon Sep 17 00:00:00 2001 From: Jun Date: Tue, 27 Dec 2022 10:00:28 +0800 Subject: [PATCH 1/2] 2022-12-27nothing updates --- public/archives/index.html | 4 - public/categories/category/index.html | 46 ----- public/categories/category/index.xml | 12 -- public/categories/index.html | 49 +---- public/categories/subcategory/index.html | 46 ----- public/categories/subcategory/index.xml | 12 -- public/index.html | 46 ----- public/index.xml | 12 -- public/resource/index.html | 46 ----- public/resource/index.xml | 12 -- public/sitemap.xml | 3 - public/tags/drosophila/index.html | 46 ----- public/tags/drosophila/index.xml | 12 -- public/tags/gentics/index.html | 46 ----- public/tags/gentics/index.xml | 12 -- public/tags/index.html | 228 ++++++++++------------- public/tags/mouse/index.html | 46 ----- public/tags/mouse/index.xml | 12 -- public/tags/zebrafish/index.html | 46 ----- public/tags/zebrafish/index.xml | 12 -- 20 files changed, 98 insertions(+), 650 deletions(-) diff --git a/public/archives/index.html b/public/archives/index.html index 21a17bd..256b61c 100644 --- a/public/archives/index.html +++ b/public/archives/index.html @@ -327,10 +327,6 @@
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content/resource/Imagingtools.md | 25 +++++++++--- content/resource/cellularmetabolism.md | 5 ++- content/resource/molecularbiology.md | 22 +++++++++-- public/2018/09/25/index.en-us/index.html | 2 +- .../2019/05/01/r-plot-2d-density/index.html | 2 +- .../index.html | 2 +- .../index.html | 2 +- public/2022/08/15/index.en-us/index.html | 2 +- .../index.html | 2 +- public/categories/by-jun-liszt-li/index.html | 2 +- public/categories/category/index.html | 2 +- public/categories/cibr-talks/index.html | 2 +- public/categories/cibr/index.html | 2 +- public/categories/index.html | 2 +- public/categories/pku/index.html | 2 +- public/categories/r/index.html | 2 +- public/categories/subcategory/index.html | 2 +- public/index.html | 2 +- public/page/about/index.html | 2 +- public/page/contact/index.html | 2 +- public/page/index.html | 2 +- public/page/publication/index.html | 2 +- public/page/research/index.html | 2 +- public/posts/index.html | 2 +- public/resource/biochemistrytools/index.html | 2 +- public/resource/cellularmetabolism/index.html | 6 ++- public/resource/clearingmethod/index.html | 2 +- public/resource/geneticstools/index.html | 2 +- public/resource/genomicsngs/index.html | 2 +- public/resource/imagingtools/index.html | 38 +++++++++--------- public/resource/index.html | 2 +- public/resource/massspectrometry/index.html | 2 +- public/resource/molecularbiology/index.html | 39 ++++++++++++------- public/resource/programing/index.html | 2 +- public/tags/2d-density/index.html | 2 +- public/tags/aav-engineering/index.html | 2 +- public/tags/atlas/index.html | 2 +- public/tags/brain-atlas/index.html | 2 +- public/tags/brain-metabolic-system/index.html | 2 +- public/tags/brain-oscillation/index.html | 2 +- .../tags/brain-vasculature-system/index.html | 2 +- public/tags/calcium-imaging/index.html | 2 +- public/tags/cell-biology/index.html | 2 +- public/tags/cell-signaling/index.html | 2 +- public/tags/cibr-talk/index.html | 2 +- public/tags/cibr/index.html | 2 +- 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inverted confocal microscope. The system is equipped with 7 laser lines 405nm, 458nm, 488nm, 514 nm, 561nm, 594nm and 633 nm. The newly designed excitation beam is elongated in y which acquires four lines of image information instead of only one with just one horizontal scanner movement. [[Standard Operation Protocol](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/ES8YWy6g61VJpaYUmIcrrG4Bq9dhrJ25WxYgI4cMiapkXQ?e=dLf0sO) | [Detailed Configuration](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EfIoB6QWG2xBglZICiWEwR0BYDtYKGV1VDXC8iwCIuNMeQ?e=duyppQ)] + +#### Information about Microscope Objectives +- Please refer to the following inventory for all of the objectives available at the [Janelia imaging core facility](https://www.janelia.org/support-team/light-microscopy/resources). [[Inventory list](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EX0KqlaSJAhHhH-vXt23DrQBmQ-MBuEJ_ELtydJhkT-9VA?e=JJdRZq) | [Zeiss confocal objectives](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EWUOJGSGpkdKgNuo0bpumCcB-o_zM3hhNK39CEPT-Fc_3A?e=kAh7z0)] +- Manufacturer brochures for objective types can also be viewed: [Zeiss Objectives](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EWtf_iuyHhFPvd8YoaBEJO8BLWgJ5C_Fqbl1vakkVVi2ag?e=BKLcaB), [Nikon Objectives](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EVhT7WvjQLRMsDdFnJ7Je9wBwNQtOV1XTq7fxCTMJ59MVA?e=NnomWS), and [Olympus Objectives](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EbWytDbHvWVBnvz9W484lvUB8eWBxy98abCpNoJtETaJOA?e=NYHfoF). + + +#### SpectraViewers +- [Fluorescence SpectraViewer](https://www.thermofisher.cn/order/fluorescence-spectraviewer#!/), ThermoFisher [[Manual](https://www.thermofisher.cn/cn/en/home/life-science/cell-analysis/fluorophores/guide-fluorescence-spectraviewer.html)] +- [SearchLight](https://searchlight.semrock.com/), Semrock [[Manual](https://searchlight.semrock.com/Help.aspx)] +- [Fluorescence Spectrum Viewer](https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer), BD Biosciences: The BD Spectrum Viewer is a tool that depicts the excitation and emission curves of fluorochromes common to flow cytometry. This tool can be used to determine appropriate filters to detect a fluorochrome as well as fluorochrome compatibility and fluorescent spillover. + + +#### Staining +- [Fluorescence Dye and Filter Database](https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/dyes/), Zeiss +- [Cell Staining Simulation Tool](https://www.thermofisher.cn/cn/en/home/life-science/lab-data-management-analysis-software/lab-apps/cell-staining-tool.html?adobe_mc=MCMID%7C32915283128660043822078872448289181953%7CMCAID%3D318B7E039CF16E3C-60001D2314A987EE%7CMCORGID%3D5B135A0C5370E6B40A490D44%40AdobeOrg%7CTS=1614293705), Life Technologies + + --- # Two-Photon Microscopy ![2pobjective25x](/img/IMG_20191125_015442_edited.jpg) @@ -74,6 +91,7 @@ In this talk, two-photon microscopy-which uses strong pulsed infrared lasers to --- + # Light-sheet Microscopy ![lightsheet001](/img/536fbb_e905706e774b47b19e8802138342a844_mv2.webp)\ ![tlsm002](/img/Dingtalk_20220903141016.jpg) @@ -148,10 +166,6 @@ During 20 years of friendship, Betzig and Hess collaborated to create the first Thomas Reese studies synaptic structure and function using advanced light and electron microscopy techniques. John Heuser developed and spent his career using quick-freeze, deep-etch electron microscopy to study all aspects of cell biology.\ - **Imaging Synaptic Vesicle Transmission**: Two pioneering electron microscopists, John Heuser and Tom Reese, reminisce about their early attempts to image synaptic vesicle transmission. (**iBiology interview**, recorded in July 2015: [[Part 1: Imaging synaptic vesicle transmission](https://pkueducn-my.sharepoint.com/:v:/g/personal/lijun0705_pku_edu_cn/EQITWJRLtSFKg5tqfCGYYC0BWzaz7CQK-HwYTtVZLXM9QA?e=I3wBVq) | [Part 2: The Future of Electron Microscopy](https://pkueducn-my.sharepoint.com/:v:/g/personal/lijun0705_pku_edu_cn/EVsGt6E9HN5Cuvfk9zGInF0BCLrcb1P8cZMgwN9UZDU_Xw?e=bm14gg) | [Part 3: Why Collaborate?](https://pkueducn-my.sharepoint.com/:v:/g/personal/lijun0705_pku_edu_cn/ES-O26Hl429Ilc5a9L6KqAUB7F86M2nlDTxsq1KlX6qNqw?e=OcweNL) | [Source](https://www.ibiology.org/neuroscience/imaging-synaptic-vesicle-transmission/#part-1)]) -======= -**Ref labs**: [Thomas Reese Lab, National Institutes of Health]()\ -[John Heuser Lab, Washington University in St. Louis]()\ ->>>>>>> Stashed changes @@ -194,7 +208,8 @@ Thomas Reese studies synaptic structure and function using advanced light and el 3. Cellfinder: https://github.com/SainsburyWellcomeCentre/cellfinder 4. BrainRender: https://github.com/BrancoLab/BrainRender 5. Allen Developing Brain atlas: https://developingmouse.brain-map.org/static/atlas - +6. NeuronStudio: A free platform that provides tools for 2D/3D visualization as well as manual and automatic detection/tracing of dendritic structures. [Link](http://research.mssm.edu/cnic/tools-ns.html) +7. yEd: A free, open-source, cross-platform graph editor used to quickly and effectively generate high-quality diagrams. --- diff --git a/content/resource/cellularmetabolism.md b/content/resource/cellularmetabolism.md index f5e0f60..2222113 100644 --- a/content/resource/cellularmetabolism.md +++ b/content/resource/cellularmetabolism.md @@ -35,8 +35,10 @@ The citric acid cycle is at the center of cellular metabolism. It plays a starri - Goldstein, Joseph L., and Michael S. Brown. “A Century of Cholesterol and Coronaries: From Plaques to Genes and Statins.” Cell 161, no. 1 (2015): 161–72. [[PDF](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EVmM9yqeT0lPuIKaqQlzvkwB0cx7RREjxtfaEAEvtxji5A?e=gxGANA) | [PMID: 25815993](https://pubmed.ncbi.nlm.nih.gov/25815993/) | [DOI Link](https://doi.org/10.1016/j.cell.2015.01.036)] -3. **Mitochondria role in Energy metabolism** +3. **Regulation of Energy metabolism** - Review in 1979: [METABOLITE TRANSPORT IN MITOCHONDRIA](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EeWcvgdnEsJHqnu77uAAWGoBq6d7dUWpyr1Kromjrev6Rg?e=ylv6xV) + - internal energy stress sensing and response program in brain ? + 4. **Lysosome role in metabolic signalling** - Review in 2019: **The lysosome as a cellular centre for signalling, metabolism and quality control** (Rosalie and Roberto, 2019) [[PDF](https://pkueducn-my.sharepoint.com/:b:/g/personal/lijun0705_pku_edu_cn/EcboMO4gKndOlJOzXWV8ohEBy6mrjgr8mpQGztgPqgYttw?e=yOvXBj) | [PMID: 30602725](https://pubmed.ncbi.nlm.nih.gov/30602725/) | [DOI Link](https://doi.org/10.1038/s41556-018-0244-7)] @@ -66,6 +68,7 @@ Sensors for the mTORC1 Pathway** (Wolfson and Sabatini, 2017) [[PDF](https://pku - How amino acids were sensed? More amino acid sensors? - How different or similar the amino acid and nutrient inputs are that drive mTORC1/TORC1 signaling in diverse organisms? - Is GATOR2 the nutrient sensing hub complex ? How does it work to integrate multiple nutrient inputs? + - How does brain sense the stress of energy supply and initiate its response program? --- diff --git a/content/resource/molecularbiology.md b/content/resource/molecularbiology.md index 94fb2a8..76dee2c 100644 --- a/content/resource/molecularbiology.md +++ b/content/resource/molecularbiology.md @@ -163,6 +163,12 @@ fuoresces in mammalian cells and tissues. miRFP718nano has maximal emission at 7 - dLight[[dLight1.2](https://doi.org/10.1126/science.aat4422), [addgene](https://www.addgene.org/111068/)] - **Histamine**: - **eCB**: + +**| Engineered neuropeptide sensors** +- **Oxytocin (OT)**: + - GRAB-OT[[GRAB-OT1.0](https://doi.org/10.1038/s41587-022-01561-2)] + + - **Glucose**: - iGlucoSnFR: A genetically encoded single-wavelength sensor for imaging cytosolic glucose [[PMID: 34161775](https://pubmed.ncbi.nlm.nih.gov/34161775/) | [DOI Link](https://doi.org/10.1016/j.celrep.2021.109284)] - **ATP**: @@ -225,11 +231,19 @@ fuoresces in mammalian cells and tissues. miRFP718nano has maximal emission at 7 --- ### DNA target Cas9 based -- **Knock out**: SpCas9, SaCas9 -- DNA base editor: dCas9-xxx -- Transcriptional activator: +> Because of the simplicity with which gRNAs may be designed and the capacity to change practically any genomic locus, CRISPR is a powerful tool for genetic screening investigations. +--- -- Cas9 based whole-genome scale pool screen +- **Knock out**: SpCas9, SaCas9 +- **DNA base editor**: dCas9-xxx +- **Transcriptional activator**: dCas9-Suntag system + +- **Cas9 based whole-genome scale pool screen** + - **CRISPR Pooled Libraries**: Pooled CRISPR libraries are made up of hundreds of plasmids, each holding several gRNAs for each target gene. Target cells are treated with the pooled library in a CRISPR screening experiment to generate a population of mutant cells, which are then screened for a phenotypic of interest. Experiments with a pooled CRISPR library are significantly more complicated than utilizing CRISPR to change a single genetic region. [[Addgene Link](https://www.addgene.org/crispr/libraries/)] + - Human Activity-Optimized CRISPR Knockout Library (3 sub-libraries in lentiCRISPRv1) [[Addgene:#1000000100](https://www.addgene.org/pooled-library/sabatini-crispr-human-high-activity-3-sublibraries/) | [DOI Link](https://doi.org/10.1126/science.aac7041) | [PMID: 26472758](https://pubmed.ncbi.nlm.nih.gov/26472758/) | Sabatini/Lander lab] + - Human CRISPR Knockout Pooled Library (GeCKO v2) [Addgene:#1000000048,#1000000049]() + - Human CRISPR Metabolic Gene Knockout Library [[Addgene:#110066](https://www.addgene.org/pooled-library/sabatini-human-crispr-metabolic-knockout/) | [DOI Link](https://doi.org/10.1016/j.cell.2015.07.016) | [PMID: 26232224](https://pubmed.ncbi.nlm.nih.gov/26232224/) | Sabatini lab] + - Drosophila Cell CRISPR Knockout Library [[Addgene:#134582,#134583,#134584](https://www.addgene.org/pooled-library/perrimon-drosophila-crispr-knockout/) | [DOI Link](https://doi.org/10.7554/elife.36333) | [PMID: 30051818](https://pubmed.ncbi.nlm.nih.gov/30051818/) | Norbert Perrimon lab] ### RNA target Cas13 based diff --git a/public/2018/09/25/index.en-us/index.html b/public/2018/09/25/index.en-us/index.html index 9ab1ac2..33bbdc5 100644 --- a/public/2018/09/25/index.en-us/index.html +++ b/public/2018/09/25/index.en-us/index.html @@ -670,7 +670,7 @@
    - © 2022 Jun-Liszt Li. All Rights Reserved + © 2023 Jun-Liszt Li. All Rights Reserved
    diff --git a/public/2019/05/01/r-plot-2d-density/index.html b/public/2019/05/01/r-plot-2d-density/index.html index 424dee3..66eeb24 100644 --- a/public/2019/05/01/r-plot-2d-density/index.html +++ b/public/2019/05/01/r-plot-2d-density/index.html @@ -603,7 +603,7 @@

    Reference
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    diff --git a/public/2019/06/14/the-origin-and-function-of-brain-oscillation/index.html b/public/2019/06/14/the-origin-and-function-of-brain-oscillation/index.html index fedda6b..5205db2 100644 --- a/public/2019/06/14/the-origin-and-function-of-brain-oscillation/index.html +++ b/public/2019/06/14/the-origin-and-function-of-brain-oscillation/index.html @@ -684,7 +684,7 @@

    References

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    diff --git a/public/2020/04/28/tailoring-the-brain-connectivity-atlas-reconstruction-and-revision-of-large-scale-microscopic-brain-atlas/index.html b/public/2020/04/28/tailoring-the-brain-connectivity-atlas-reconstruction-and-revision-of-large-scale-microscopic-brain-atlas/index.html index a48866a..d370739 100644 --- a/public/2020/04/28/tailoring-the-brain-connectivity-atlas-reconstruction-and-revision-of-large-scale-microscopic-brain-atlas/index.html +++ b/public/2020/04/28/tailoring-the-brain-connectivity-atlas-reconstruction-and-revision-of-large-scale-microscopic-brain-atlas/index.html @@ -534,7 +534,7 @@

    References

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    diff --git a/public/2022/08/15/index.en-us/index.html b/public/2022/08/15/index.en-us/index.html index 97c47bf..2a944ed 100644 --- a/public/2022/08/15/index.en-us/index.html +++ b/public/2022/08/15/index.en-us/index.html @@ -666,7 +666,7 @@

    Reference
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    diff --git a/public/2022/12/22/protocol-for-calcium-imaging-of-free-moving-mice/index.html b/public/2022/12/22/protocol-for-calcium-imaging-of-free-moving-mice/index.html index 3cc8764..0aaf40d 100644 --- a/public/2022/12/22/protocol-for-calcium-imaging-of-free-moving-mice/index.html +++ b/public/2022/12/22/protocol-for-calcium-imaging-of-free-moving-mice/index.html @@ -498,7 +498,7 @@

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    Hello, My name is Jun-Liszt Li. my science inter
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    Hello, My name is Jun-Liszt Li. my science inter
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    diff --git a/public/categories/cibr/index.html b/public/categories/cibr/index.html index d408111..5ee1717 100644 --- a/public/categories/cibr/index.html +++ b/public/categories/cibr/index.html @@ -314,7 +314,7 @@
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    Academic Publications

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    Molecular engineering

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    diff --git a/public/posts/index.html b/public/posts/index.html index 8b6050a..83dde63 100644 --- a/public/posts/index.html +++ b/public/posts/index.html @@ -718,7 +718,7 @@

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    Nobel laureates

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    diff --git a/public/resource/cellularmetabolism/index.html b/public/resource/cellularmetabolism/index.html index fb7b486..b47b8fc 100644 --- a/public/resource/cellularmetabolism/index.html +++ b/public/resource/cellularmetabolism/index.html @@ -378,9 +378,10 @@

  • -

    Mitochondria role in Energy metabolism

    +

    Regulation of Energy metabolism

  • @@ -422,6 +423,7 @@

  • How amino acids were sensed? More amino acid sensors?
  • How different or similar the amino acid and nutrient inputs are that drive mTORC1/TORC1 signaling in diverse organisms?
  • Is GATOR2 the nutrient sensing hub complex ? How does it work to integrate multiple nutrient inputs?
    • +
  • How does brain sense the stress of energy supply and initiate its response program?

    • @@ -625,7 +627,7 @@

    Ref. Database

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    diff --git a/public/resource/clearingmethod/index.html b/public/resource/clearingmethod/index.html index b6db3fb..2bfaf78 100644 --- a/public/resource/clearingmethod/index.html +++ b/public/resource/clearingmethod/index.html @@ -501,7 +501,7 @@

    Reference

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    diff --git a/public/resource/geneticstools/index.html b/public/resource/geneticstools/index.html index 0d596df..e454850 100644 --- a/public/resource/geneticstools/index.html +++ b/public/resource/geneticstools/index.html @@ -688,7 +688,7 @@

    Nobel laureates

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    diff --git a/public/resource/genomicsngs/index.html b/public/resource/genomicsngs/index.html index 7614cda..8a25284 100644 --- a/public/resource/genomicsngs/index.html +++ b/public/resource/genomicsngs/index.html @@ -584,7 +584,7 @@

    Nobel laureates

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    diff --git a/public/resource/imagingtools/index.html b/public/resource/imagingtools/index.html index a6fdf34..cf79c70 100644 --- a/public/resource/imagingtools/index.html +++ b/public/resource/imagingtools/index.html @@ -367,6 +367,22 @@

    Confocal Microscopy

    Commercial

    Leica: Leica-SP8 (Archived Product Replaced by STELLARIS 5 & STELLARIS 8)
    Carl Zeiss: LSM 880, with Airyscan: The Zeiss LSM880 with Ariyscan 2 is an inverted confocal microscope. The system is equipped with 7 laser lines 405nm, 458nm, 488nm, 514 nm, 561nm, 594nm and 633 nm. The newly designed excitation beam is elongated in y which acquires four lines of image information instead of only one with just one horizontal scanner movement. [Standard Operation Protocol | Detailed Configuration]

    +

    Information about Microscope Objectives

    + +

    SpectraViewers

    +
      +
    • Fluorescence SpectraViewer, ThermoFisher [Manual]
      • +
    • SearchLight, Semrock [Manual]
      • +
    • Fluorescence Spectrum Viewer, BD Biosciences: The BD Spectrum Viewer is a tool that depicts the excitation and emission curves of fluorochromes common to flow cytometry. This tool can be used to determine appropriate filters to detect a fluorochrome as well as fluorochrome compatibility and fluorescent spillover.
      • +
    +

    Staining

    +

    Two-Photon Microscopy

    2pobjective25x

    @@ -470,24 +486,6 @@

    Electron Microscopy

    -

    ======= -Ref labs: Thomas Reese Lab, National Institutes of Health
    -John Heuser Lab, Washington University in St. Louis\

    -
    -
    -
    -
    -
    -
    -
    -

    Stashed changes

    -
    -
    -
    -
    -
    -
    -

    Croy-Electron Microscopy(cryo-EM)

    Ref labs:

      @@ -537,6 +535,8 @@

      Open Source Softwares

    1. Cellfinder: https://github.com/SainsburyWellcomeCentre/cellfinder
    2. BrainRender: https://github.com/BrancoLab/BrainRender
    3. Allen Developing Brain atlas: https://developingmouse.brain-map.org/static/atlas
      4. +
    4. NeuronStudio: A free platform that provides tools for 2D/3D visualization as well as manual and automatic detection/tracing of dendritic structures. Link
      5. +
    5. yEd: A free, open-source, cross-platform graph editor used to quickly and effectively generate high-quality diagrams.

    Nobel laureates

    @@ -633,7 +633,7 @@

    Nobel laureates

    - © 2022 Jun-Liszt Li. All Rights Reserved + © 2023 Jun-Liszt Li. All Rights Reserved
    diff --git a/public/resource/index.html b/public/resource/index.html index 08e3d56..868b663 100644 --- a/public/resource/index.html +++ b/public/resource/index.html @@ -725,7 +725,7 @@

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    diff --git a/public/resource/massspectrometry/index.html b/public/resource/massspectrometry/index.html index bf9cb40..0356285 100644 --- a/public/resource/massspectrometry/index.html +++ b/public/resource/massspectrometry/index.html @@ -605,7 +605,7 @@

    Online courses

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    diff --git a/public/resource/molecularbiology/index.html b/public/resource/molecularbiology/index.html index c125c0a..e8bc14f 100644 --- a/public/resource/molecularbiology/index.html +++ b/public/resource/molecularbiology/index.html @@ -535,21 +535,23 @@

    Genetically encoded indicators

    | Engineered neurotransmitter sensors

    +

    | Engineered neuropeptide sensors

    +
    • -

      Histamine:

      -
      • -
    • -

      eCB:

      +

      Oxytocin (OT):

      +

    • Glucose:

      @@ -637,18 +639,29 @@

      CRISPR systems

      DNA target Cas9 based

      +
      +

      Because of the simplicity with which gRNAs may be designed and the capacity to change practically any genomic locus, CRISPR is a powerful tool for genetic screening investigations.

      +
      +

      • Knock out: SpCas9, SaCas9

      • -

        DNA base editor: dCas9-xxx

        +

        DNA base editor: dCas9-xxx

      • -

        Transcriptional activator:

        +

        Transcriptional activator: dCas9-Suntag system

      • -

        Cas9 based whole-genome scale pool screen

        +

        Cas9 based whole-genome scale pool screen

        +
          +
        • CRISPR Pooled Libraries: Pooled CRISPR libraries are made up of hundreds of plasmids, each holding several gRNAs for each target gene. Target cells are treated with the pooled library in a CRISPR screening experiment to generate a population of mutant cells, which are then screened for a phenotypic of interest. Experiments with a pooled CRISPR library are significantly more complicated than utilizing CRISPR to change a single genetic region. [Addgene Link]
          • +
        • Human Activity-Optimized CRISPR Knockout Library (3 sub-libraries in lentiCRISPRv1) [Addgene:#1000000100 | DOI Link | PMID: 26472758 | Sabatini/Lander lab]
          • +
        • Human CRISPR Knockout Pooled Library (GeCKO v2) Addgene:#1000000048,#1000000049
          • +
        • Human CRISPR Metabolic Gene Knockout Library [Addgene:#110066 | DOI Link | PMID: 26232224 | Sabatini lab]
          • +
        • Drosophila Cell CRISPR Knockout Library [Addgene:#134582,#134583,#134584 | DOI Link | PMID: 30051818 | Norbert Perrimon lab]
          • +

      RNA target Cas13 based

      @@ -1062,7 +1075,7 @@

      Nobel laureates

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      sc-ATACseq datasets

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      References

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      Vendor instruments