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setup_qiime2
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setup_qiime2
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#!/usr/bin/env python3
"""Set up Qiime 2 on Google colab.
Do not use this on o local machine, especially not as an admin!
"""
import os
import sys
import shutil
from subprocess import Popen, PIPE
r = Popen(["pip", "install", "rich"])
r.wait()
from rich.console import Console # noqa
con = Console()
has_conda = "conda version" in os.popen("conda info").read()
has_qiime = "QIIME 2 release:" in os.popen("qiime info").read()
MINICONDA_PATH = (
"https://repo.anaconda.com/miniconda/Miniconda3-latest-Linux-x86_64.sh"
)
QIIME_YAML_TEMPLATE = (
"https://data.qiime2.org/distro/core/qiime2-{version}-py{python}-linux-conda.yml"
)
if len(sys.argv) == 2:
version = sys.argv[1]
else:
version = "2022.8"
if tuple(float(v) for v in version.split(".")) < (2021, 4):
pyver = "36"
else:
pyver = "38"
QIIME_YAML_URL = QIIME_YAML_TEMPLATE.format(version=version, python=pyver)
QIIME_YAML = os.path.basename(QIIME_YAML_URL)
def cleanup():
"""Remove downloaded files."""
if os.path.exists("Miniconda3-latest-Linux-x86_64.sh"):
os.remove("Miniconda3-latest-Linux-x86_64.sh")
if os.path.exists(QIIME_YAML):
os.remove(QIIME_YAML)
if os.path.exists("/content/sample_data"):
shutil.rmtree("/content/sample_data")
con.log(":broom: Cleaned up unneeded files.")
def run_and_check(args, check, message, failure, success, console=con):
"""Run a command and check that it worked."""
console.log(message)
r = Popen(args, env=os.environ, stdout=PIPE, stderr=PIPE,
universal_newlines=True)
o, e = r.communicate()
out = o + e
if r.returncode == 0 and check in out:
console.log("[blue]%s[/blue]" % success)
else:
console.log("[red]%s[/red]" % failure, out)
cleanup()
sys.exit(1)
if __name__ == "__main__":
if not has_conda:
run_and_check(
["wget", MINICONDA_PATH],
"saved",
":snake: Downloading miniconda...",
"failed downloading miniconda :sob:",
":snake: Done."
)
run_and_check(
["bash", "Miniconda3-latest-Linux-x86_64.sh", "-bfp", "/usr/local"],
"installation finished.",
":snake: Installing miniconda...",
"could not install miniconda :sob:",
":snake: Installed miniconda to `/usr/local`."
)
else:
con.log(":snake: Miniconda is already installed. Skipped.")
if not has_qiime:
run_and_check(
["wget", QIIME_YAML_URL],
"saved",
":mag: Downloading Qiime 2 package list...",
"could not download package list :sob:",
":mag: Done."
)
run_and_check(
["conda", "env", "update", "-n", "base", "--file", QIIME_YAML],
"To activate this environment, use",
":mag: Installing Qiime 2. This may take a little bit.\n :clock1:",
"could not install Qiime 2 :sob:",
":mag: Done."
)
run_and_check(
["pip", "install", "empress"],
"Successfully installed empress-",
":evergreen_tree: Installing Empress...",
"could not install Empress :sob:",
":evergreen_tree: Done."
)
else:
con.log(":mag: Qiime 2 is already installed. Skipped.")
run_and_check(
["qiime", "info"],
"QIIME 2 release:",
":bar_chart: Checking that Qiime 2 command line works...",
"Qiime 2 command line does not seem to work :sob:",
":bar_chart: Qiime 2 command line looks good :tada:"
)
if sys.version_info[0:2] == (int(pyver[0]), int(pyver[1])):
sys.path.append("/usr/local/lib/python3.{}/site-packages".format(pyver[1]))
con.log(":mag: Fixed import paths to include Qiime 2.")
con.log(":bar_chart: Checking if Qiime 2 import works...")
try:
import qiime2 # noqa
except Exception:
con.log("[red]Qiime 2 can not be imported :sob:[/red]")
sys.exit(1)
con.log("[blue]:bar_chart: Qiime 2 can be imported :tada:[/blue]")
cleanup()
con.log("[green]Everything is A-OK. "
"You can start using Qiime 2 now :thumbs_up:[/green]")