diff --git a/PRESUBMIT.py b/PRESUBMIT.py
index 370bca4241e0c0..c49ccd0c4f895c 100644
--- a/PRESUBMIT.py
+++ b/PRESUBMIT.py
@@ -1691,7 +1691,6 @@ def GetTryServerMasterForBot(bot):
   """
   # Potentially ambiguous bot names are listed explicitly.
   master_map = {
-      'win_gpu': 'tryserver.chromium.gpu',
       'chromium_presubmit': 'tryserver.chromium.linux',
       'blink_presubmit': 'tryserver.chromium.linux',
       'tools_build_presubmit': 'tryserver.chromium.linux',
diff --git a/PRESUBMIT_test.py b/PRESUBMIT_test.py
index 872545ebaa7940..5c93d982a76678 100755
--- a/PRESUBMIT_test.py
+++ b/PRESUBMIT_test.py
@@ -691,10 +691,6 @@ def testFailure(self):
 class TryServerMasterTest(unittest.TestCase):
   def testTryServerMasters(self):
     bots = {
-        'tryserver.chromium.gpu': [
-            'win_gpu',
-            'win_gpu_triggered_tests',
-        ],
         'tryserver.chromium.mac': [
             'ios_dbg_simulator',
             'ios_rel_device',
diff --git a/gpu/PRESUBMIT.py b/gpu/PRESUBMIT.py
deleted file mode 100644
index 53fc1535505007..00000000000000
--- a/gpu/PRESUBMIT.py
+++ /dev/null
@@ -1,16 +0,0 @@
-# Copyright (c) 2014 The Chromium Authors. All rights reserved.
-# Use of this source code is governed by a BSD-style license that can be
-# found in the LICENSE file.
-
-"""Top-level presubmit script for gpu/.
-
-See http://dev.chromium.org/developers/how-tos/depottools/presubmit-scripts
-for more details about the presubmit API built into depot_tools.
-"""
-
-def GetPreferredTryMasters(project, change):
-  return {
-    'tryserver.chromium.gpu': {
-      'win_gpu': set(['defaulttests']),
-    }
-  }