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Clarifying questions on published materials #2
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Hi
Correct. You can use MultiBac with any organism
MultiBaC has been designed to correct the batch effect across omics types to favor multiomics data integration. You are expected to integrate several omics matrices, not just one. The most simple scenario is to have three different omics types distributed in two batches, with one of the omics types shared between the two batches.
Normalized data are requested because the batch correction process will return corrected values that are no longer counts. Using TPM values is fine. If you see large distribution differences among samples within the same batch, then TMM is also recommended, but if this is not a problem, just TPM would be fine. Hope this helps Ana |
@AnaConesa, thank you for your detailed and quick response! |
Hi In this case you should use the ARSyN function of the MultiBac package. You can use or not the information about the batches. Also it works better when you have a multifactorial design. |
@AnaConesa, thank you! |
Yes, provided that you can normalized data, not count data. |
Thank you! |
Hello! I am very interested in the approach developed by your team, but after reading the available materials, several questions remain:
Thanks!
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