One of the critical steps in a genome sequencing project is to assess the completeness of the predicted gene set. The standard workflow starts with the identification of a set of core genes for the taxonomic group, in which the target species belongs to. The fraction of missing core genes serves then as a proxy of the target gene set completeness.
fCAT is a feature-aware Completeness Assessment Tool, that helps to answer the question "How complete is my gene set?". In particularly, fCAT checks for the presence of conserved genes (the core genes) of a specific taxonomy clade in the target gene set using feature-aware directed ortholog search fDOG. In addition to the length criteria for classifying the found orthologs (as same as BUSCO), fCAT utilizes the domain architecture similarity FAS scores to further validate the orthologs. The later gives an alternative view on the accuracy of the target gene models, which shows how different the target orthologs in comparison to the core genes in their domain architecture.
fCAT outputs both the summary result in a tabular text file and the phylogenetic profile of the core genes, which can be visualized using the tool PhyloProfile. By analyzing the profiles of the entire orthologous groups within a specific taxonomy clade, we can further identify and ultimately correct erroneous gene annotations.
Click here for the full PDF version of the ECCB2022 poster
fCAT tool is distributed as a python package called fcat. It is compatible with Python ≥ v3.9.
You can install fcat using pip:
python3 -m pip install fcat
or, in case you do not have admin rights, and don't use package systems like Anaconda to manage environments you need to use the --user option:
python3 -m pip install --user fcat
and then add the following line to the end of your ~/.bashrc or ~/.bash_profile file, restart the current terminal to apply the change (or type source ~/.bashrc):
export PATH=$HOME/.local/bin:$PATH
Note: fCAT requires R to be present! Please make sure that you have R installed on your computer.
The complete process of fCAT can be done using one function fcat
fcat --coreDir /path/to/fcat_data --coreSet eukaryota --refspecList "HOMSA@9606@2" --querySpecies /path/to/query.fa [--annoQuery /path/to/query.json] [--outDir /path/to/fcat/output]
where eukaryota is name of the fCAT core set (equivalent to BUSCO set); HOMSA@9606@2 is the reference species from that core set that will be used for the ortholog search; query is the name of species of interest. If --annoQuery not specified, fCAT fill do the feature annotation for the query proteins using FAS tool.
You will find the output in the /path/to/fcat/output/fcatOutput/eukaryota/query/ folder, where /path/to/fcat/output/ could be your current directory if you not specified --outDir when running fcat. The following important output files/folders can be found:
- all_summary.txt: summary of the completeness assessment using all 4 score modes
- all_full.txt: the complete assessment of 4 score modes in tab delimited file
- fdogOutput.tar.gz: a zipped file of the ortholog search result
- mode_1, mode_2, mode_3 and mode_4: detailed output for each score mode
- phyloprofileOutput: folder contains output phylogenetic profile data that can be used with PhyloProfile tool
Besides, if you have already run fCAT for several query taxa with the same fCAT core set, you can find the merged phylogentic profiles for all of those taxa within the corresponding core set output (e.g. /path/to/fcat/output/fcatOutput/eukaryota/*.phyloprofile).
The table below explains how the specific ortholog group cutoffs for each fCAT core set were calculated, and which value of the query ortholog is used to assess its completeness, or more precisely, its functional equivalence to the ortholog group it belongs to. If the value of a query ortholog is not less than its ortholog group cutoff, that group will be evaluated as similar or complete. In case co-orthologs have been predicted, the assessment for the core group will be duplicated. Depending on the value of each single ortholog, a duplicated group can be seen as duplicated (similar) or duplicated (dissimilar) in the full report (e.g. all_full.txt).
| Score mode | Cutoff | Value used for comparing |
|---|---|---|
| Mode 1 - Strict mode | Mean of FAS scores between all core orthologs | Mean of FAS scores between query ortholog and all core proteins |
| Mode 2 - Reference mode | Mean of FAS scores between refspec and all other core orthologs | Mean of FAS scores between query ortholog and refspec protein |
| Mode 3 - Relaxed mode | The lower bound of the confidence interval calculated by the distribution of all-vs-all FAS score in a core group | Mean of FAS scores between query ortholog and all core proteins |
| Mode 4 - Length mode | Mean and standard deviation of all core protein lengths | Length of query ortholog |
Note: FAS scores are bidirectional FAS scors; core protein or core ortholog is protein in the core ortholog groups; query protein or query ortholog is ortholog protein of query species; refspec is the specified reference species
Any bug reports or comments, suggestions are highly appreciated. Please open an issue on GitHub or be in touch via email.
Tran V and Ebersberger I. fCAT: Assessing gene set completeness using domain-architecture aware targeted ortholog searches. F1000Research 2022, 11:1091 (poster) (doi: 10.7490/f1000research.1119126.1)
For further support or bug reports please contact: tran@bio.uni-frankfurt.de